Evaluation of Violet Red Bile Glucose agar specificity for Enterobacteriaceae isolation in raw goat milk
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | por eng |
Título da fonte: | Vigilância Sanitária em Debate |
Texto Completo: | https://visaemdebate.incqs.fiocruz.br/index.php/visaemdebate/article/view/1340 |
Resumo: | Introduction: Coliforms have been used over the years as indicator microorganisms for microbiological quality. However, several studies report the lack of direct correlation between their presence and that of pathogens in the evaluation of food safety, leading to the suggestion of new analysis proposals, such as the search for total enterobacteria, which includes all members of the Enterobacteriaceae family. Objective: This study aimed to determine the population of enterobacteria in raw goat milk samples based on the microbiological criteria suggested by the National Health Surveillance Agency - Ministry of Health (Anvisa - MS) - Public Consultation Noº 542 of July 17, 2018 for Pasteurized milk samples. Method: 21 samples from small producers from different regions of the state of Rio de Janeiro were used. Isolates were obtained from inoculation on VRBG agar according to ISO 21528-2 and identified by Matrix Associated Laser Desorption-Ionization – Time of Flight (MALDI/TOF) mass spectrometry. Results: Of the 222 isolates identified, only 26.6% belonged to the Enterobacteriaceae family. Another 61.3% belonged to the Pseudomonas genus, 4.5% to the Acinetobacter genus and 7.6% were Stenotrophomonas maltophilia. Although there are no microbiological parameters in Anvisa-MS RDC Nº 12 of January 2, 2001 for raw milk, the results of isolation on VBRG agar led to the investigation of other studies with similar findings regarding isolation of VRBG bacteria from goat and bovine milk samples. Conclusions: Detection in VRBG agar of non-Enterobacteriaceae-negative oxidase bacteria, such as those of the genus Acinetobacter, suggests the need for a debate on the methodology indicated by ISO 21528-2 for enumeration of enterobacteria, as the new regulation on standard microbiological data for food will no longer use the coliform group, but the Enterobacteriaceae/g or mL count, as an indicator. |
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Evaluation of Violet Red Bile Glucose agar specificity for Enterobacteriaceae isolation in raw goat milkAvaliação da especificidade do ágar Violeta Vermelho Bile Glicose para o isolamento de Enterobacteriaceae em leite de cabra cruEnterobacteriaceaePseudomonasMilkAcinetobacterLegislationEnterobacteriaceaePseudomonasLeiteAcinetobacterLegislaçãoIntroduction: Coliforms have been used over the years as indicator microorganisms for microbiological quality. However, several studies report the lack of direct correlation between their presence and that of pathogens in the evaluation of food safety, leading to the suggestion of new analysis proposals, such as the search for total enterobacteria, which includes all members of the Enterobacteriaceae family. Objective: This study aimed to determine the population of enterobacteria in raw goat milk samples based on the microbiological criteria suggested by the National Health Surveillance Agency - Ministry of Health (Anvisa - MS) - Public Consultation Noº 542 of July 17, 2018 for Pasteurized milk samples. Method: 21 samples from small producers from different regions of the state of Rio de Janeiro were used. Isolates were obtained from inoculation on VRBG agar according to ISO 21528-2 and identified by Matrix Associated Laser Desorption-Ionization – Time of Flight (MALDI/TOF) mass spectrometry. Results: Of the 222 isolates identified, only 26.6% belonged to the Enterobacteriaceae family. Another 61.3% belonged to the Pseudomonas genus, 4.5% to the Acinetobacter genus and 7.6% were Stenotrophomonas maltophilia. Although there are no microbiological parameters in Anvisa-MS RDC Nº 12 of January 2, 2001 for raw milk, the results of isolation on VBRG agar led to the investigation of other studies with similar findings regarding isolation of VRBG bacteria from goat and bovine milk samples. Conclusions: Detection in VRBG agar of non-Enterobacteriaceae-negative oxidase bacteria, such as those of the genus Acinetobacter, suggests the need for a debate on the methodology indicated by ISO 21528-2 for enumeration of enterobacteria, as the new regulation on standard microbiological data for food will no longer use the coliform group, but the Enterobacteriaceae/g or mL count, as an indicator.Introdução: Bactérias do grupo dos coliformes têm sido empregadas ao longo dos anos como micro-organismos indicadores da qualidade microbiológica. No entanto, diversos estudos relatam a falta de correlação direta entre sua presença e a de patógenos na avaliação da segurança de alimentos, levando a sugestão de novas propostas de análise, como a pesquisa de enterobactérias totais, que compreende todos os membros da família Enterobacteriaceae. Objetivo: Determinar a população de enterobactérias em amostras de leite caprino cru com base nos critérios microbiológicos sugeridos pela Consulta Pública nº 542, de 17 de julho de 2018, da Agência Nacional de Vigilância Sanitária - Ministério da Saúde (Anvisa - MS), para amostras de leite pasteurizado. Método: Foram utilizadas 21 amostras de pequenos produtores de diversas regiões do estado do Rio de Janeiro. Os isolados foram obtidos a partir de inoculação em ágar VRBG, de acordo com a ISO 21528-2, e identificados por espectrometria de massas por Matrix Associated Laser Desorption-Ionization – Time of Flight (MALDI/TOF). Resultados: Dos 222 isolados identificados, apenas 26,6% pertenciam à família Enterobacteriaceae. Outros 61,3% pertenciam ao gênero Pseudomonas, 4,5% ao gênero Acinetobacter e 7,6% eram Stenotrophomonas maltophilia. Apesar de não haver parâmetros microbiológicos na RDC nº 12, de 2 de janeiro de 2001, da Anvisa-MS, para leite cru, os resultados do isolamento em ágar VBRG levaram à investigação de outros estudos com achados semelhantes referentes ao isolamento de bactérias VRBG a partir de amostras de leite caprino e bovino. Conclusões: A detecção em ágar VRBG de bactérias oxidase negativas não pertencentes à família Enterobacteriaceae, como as do gênero Acinetobacter, sugere a necessidade de um debate acerca da metodologia indicada pela ISO 21528-2 para enumeração de enterobactérias, visto que o novo regulamento sobre padrões microbiológicos para alimentos não mais utilizará o grupo coliforme como indicador, e sim a contagem de Enterobacteriaceae/g ou mL.Instituto Nacional de Controle de Qualidade em Saúde2020-02-27info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion"Peer-reviewed article""Artículo revisado por pares""Artigo avaliado pelos pares"application/pdfapplication/pdfhttps://visaemdebate.incqs.fiocruz.br/index.php/visaemdebate/article/view/134010.22239/2317-269X.01340Health Surveillance under Debate: Society, Science & Technology ; Vol. 8 No. 1 (2020): February; 91-96Vigilancia en Salud en Debate: Sociedad, Ciencia y Tecnología; Vol. 8 Núm. 1 (2020): Febrero; 91-96Vigil Sanit Debate, Rio de Janeiro; v. 8 n. 1 (2020): Fevereiro; 91-962317-269Xreponame:Vigilância Sanitária em Debateinstname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZporenghttps://visaemdebate.incqs.fiocruz.br/index.php/visaemdebate/article/view/1340/1133https://visaemdebate.incqs.fiocruz.br/index.php/visaemdebate/article/view/1340/1182Copyright (c) 2020 Vigilância Sanitária em Debate: Sociedade, Ciência & Tecnologia (Health Surveillance under Debate: Society, Science & Technology) – Visa em Debatehttps://creativecommons.org/licenses/by-nc-nd/4.0info:eu-repo/semantics/openAccessRamos, Gustavo Luis de Paiva AnciensNascimento, Janaína dos Santos2023-06-27T15:12:10Zoai:ojs.visaemdebate.incqs.fiocruz.br:article/1340Revistahttps://visaemdebate.incqs.fiocruz.br/index.php/visaemdebatePUBhttps://visaemdebate.incqs.fiocruz.br/index.php/visaemdebate/oaiincqs.visaemdebate@fiocruz.br || gisele.neves@fiocruz.br2317-269X2317-269Xopendoar:2023-06-27T15:12:10Vigilância Sanitária em Debate - Fundação Oswaldo Cruz (FIOCRUZ)false |
dc.title.none.fl_str_mv |
Evaluation of Violet Red Bile Glucose agar specificity for Enterobacteriaceae isolation in raw goat milk Avaliação da especificidade do ágar Violeta Vermelho Bile Glicose para o isolamento de Enterobacteriaceae em leite de cabra cru |
title |
Evaluation of Violet Red Bile Glucose agar specificity for Enterobacteriaceae isolation in raw goat milk |
spellingShingle |
Evaluation of Violet Red Bile Glucose agar specificity for Enterobacteriaceae isolation in raw goat milk Ramos, Gustavo Luis de Paiva Anciens Enterobacteriaceae Pseudomonas Milk Acinetobacter Legislation Enterobacteriaceae Pseudomonas Leite Acinetobacter Legislação |
title_short |
Evaluation of Violet Red Bile Glucose agar specificity for Enterobacteriaceae isolation in raw goat milk |
title_full |
Evaluation of Violet Red Bile Glucose agar specificity for Enterobacteriaceae isolation in raw goat milk |
title_fullStr |
Evaluation of Violet Red Bile Glucose agar specificity for Enterobacteriaceae isolation in raw goat milk |
title_full_unstemmed |
Evaluation of Violet Red Bile Glucose agar specificity for Enterobacteriaceae isolation in raw goat milk |
title_sort |
Evaluation of Violet Red Bile Glucose agar specificity for Enterobacteriaceae isolation in raw goat milk |
author |
Ramos, Gustavo Luis de Paiva Anciens |
author_facet |
Ramos, Gustavo Luis de Paiva Anciens Nascimento, Janaína dos Santos |
author_role |
author |
author2 |
Nascimento, Janaína dos Santos |
author2_role |
author |
dc.contributor.author.fl_str_mv |
Ramos, Gustavo Luis de Paiva Anciens Nascimento, Janaína dos Santos |
dc.subject.por.fl_str_mv |
Enterobacteriaceae Pseudomonas Milk Acinetobacter Legislation Enterobacteriaceae Pseudomonas Leite Acinetobacter Legislação |
topic |
Enterobacteriaceae Pseudomonas Milk Acinetobacter Legislation Enterobacteriaceae Pseudomonas Leite Acinetobacter Legislação |
description |
Introduction: Coliforms have been used over the years as indicator microorganisms for microbiological quality. However, several studies report the lack of direct correlation between their presence and that of pathogens in the evaluation of food safety, leading to the suggestion of new analysis proposals, such as the search for total enterobacteria, which includes all members of the Enterobacteriaceae family. Objective: This study aimed to determine the population of enterobacteria in raw goat milk samples based on the microbiological criteria suggested by the National Health Surveillance Agency - Ministry of Health (Anvisa - MS) - Public Consultation Noº 542 of July 17, 2018 for Pasteurized milk samples. Method: 21 samples from small producers from different regions of the state of Rio de Janeiro were used. Isolates were obtained from inoculation on VRBG agar according to ISO 21528-2 and identified by Matrix Associated Laser Desorption-Ionization – Time of Flight (MALDI/TOF) mass spectrometry. Results: Of the 222 isolates identified, only 26.6% belonged to the Enterobacteriaceae family. Another 61.3% belonged to the Pseudomonas genus, 4.5% to the Acinetobacter genus and 7.6% were Stenotrophomonas maltophilia. Although there are no microbiological parameters in Anvisa-MS RDC Nº 12 of January 2, 2001 for raw milk, the results of isolation on VBRG agar led to the investigation of other studies with similar findings regarding isolation of VRBG bacteria from goat and bovine milk samples. Conclusions: Detection in VRBG agar of non-Enterobacteriaceae-negative oxidase bacteria, such as those of the genus Acinetobacter, suggests the need for a debate on the methodology indicated by ISO 21528-2 for enumeration of enterobacteria, as the new regulation on standard microbiological data for food will no longer use the coliform group, but the Enterobacteriaceae/g or mL count, as an indicator. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-02-27 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion "Peer-reviewed article" "Artículo revisado por pares" "Artigo avaliado pelos pares" |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://visaemdebate.incqs.fiocruz.br/index.php/visaemdebate/article/view/1340 10.22239/2317-269X.01340 |
url |
https://visaemdebate.incqs.fiocruz.br/index.php/visaemdebate/article/view/1340 |
identifier_str_mv |
10.22239/2317-269X.01340 |
dc.language.iso.fl_str_mv |
por eng |
language |
por eng |
dc.relation.none.fl_str_mv |
https://visaemdebate.incqs.fiocruz.br/index.php/visaemdebate/article/view/1340/1133 https://visaemdebate.incqs.fiocruz.br/index.php/visaemdebate/article/view/1340/1182 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by-nc-nd/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-nd/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Instituto Nacional de Controle de Qualidade em Saúde |
publisher.none.fl_str_mv |
Instituto Nacional de Controle de Qualidade em Saúde |
dc.source.none.fl_str_mv |
Health Surveillance under Debate: Society, Science & Technology ; Vol. 8 No. 1 (2020): February; 91-96 Vigilancia en Salud en Debate: Sociedad, Ciencia y Tecnología; Vol. 8 Núm. 1 (2020): Febrero; 91-96 Vigil Sanit Debate, Rio de Janeiro; v. 8 n. 1 (2020): Fevereiro; 91-96 2317-269X reponame:Vigilância Sanitária em Debate instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
instname_str |
Fundação Oswaldo Cruz (FIOCRUZ) |
instacron_str |
FIOCRUZ |
institution |
FIOCRUZ |
reponame_str |
Vigilância Sanitária em Debate |
collection |
Vigilância Sanitária em Debate |
repository.name.fl_str_mv |
Vigilância Sanitária em Debate - Fundação Oswaldo Cruz (FIOCRUZ) |
repository.mail.fl_str_mv |
incqs.visaemdebate@fiocruz.br || gisele.neves@fiocruz.br |
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1797042045421879296 |