Optimization and intralaboratorial validation of an analytical method by HPLC/UV for identification and quantification of p-phenylenediamine in henna dye hair and eyebrows
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | por eng |
Título da fonte: | Vigilância Sanitária em Debate |
Texto Completo: | https://visaemdebate.incqs.fiocruz.br/index.php/visaemdebate/article/view/1265 |
Resumo: | Introduction: p-Phenylenediamine (PPD), aromatic sensitizing amine, has been added to henna powder to modify its natural color to black, increasing its fixation time, a practice that is prohibited in eyelash and eyebrows dyes. Objective: The objective of this study was to optimize and validate, at an intra-laboratory level, an analytical method by HPLC/UV for identification and quantification of PPD in hen dyes for hair and eyebrows. Method: In the method, C8 reverse phase column, mobile phase 1% triethanolamine (pH 8.4) andacetonitrile (99: 1, v/v), detection 280nm, injection volume: 10uL, flow 1.0 mL/min, column temperature 32°C, run time 10 min, linearity 5-45 μg/mL (n = 5) with correlation coefficient 0.9982, were used. For Cochran test (homoscedasticity): 0.3350 and Critical (0.3934) with 99% confidence. Limits of detection 1.17 μg/mL and quantification 3.54 μg/mL. The coefficient of variation of repeatability was 0.12% and the intermediate precision by F-test yielded p value of 0.283 with 95% of confidence. Accuracy results comprised acceptance criteria of 90%-107%. Results: Of the 19 analyzed samples, 14 presented PPD content between 1.74 and 3.65% w/w, in disagreement with Legislation. Conclusions: The proposed method can contribute to monitoring of quality and safety of use of these products. |
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Optimization and intralaboratorial validation of an analytical method by HPLC/UV for identification and quantification of p-phenylenediamine in henna dye hair and eyebrowsOtimização e validação intralaboratorial de método analítico por CLAE/UV para identificação e quantificação de p-fenilenodiamina em tinturas de hena para cabelos e sobrancelhasp-fenilenodiaminaHennaMethod ValidationPPDHenaValidação de MétodoIntroduction: p-Phenylenediamine (PPD), aromatic sensitizing amine, has been added to henna powder to modify its natural color to black, increasing its fixation time, a practice that is prohibited in eyelash and eyebrows dyes. Objective: The objective of this study was to optimize and validate, at an intra-laboratory level, an analytical method by HPLC/UV for identification and quantification of PPD in hen dyes for hair and eyebrows. Method: In the method, C8 reverse phase column, mobile phase 1% triethanolamine (pH 8.4) andacetonitrile (99: 1, v/v), detection 280nm, injection volume: 10uL, flow 1.0 mL/min, column temperature 32°C, run time 10 min, linearity 5-45 μg/mL (n = 5) with correlation coefficient 0.9982, were used. For Cochran test (homoscedasticity): 0.3350 and Critical (0.3934) with 99% confidence. Limits of detection 1.17 μg/mL and quantification 3.54 μg/mL. The coefficient of variation of repeatability was 0.12% and the intermediate precision by F-test yielded p value of 0.283 with 95% of confidence. Accuracy results comprised acceptance criteria of 90%-107%. Results: Of the 19 analyzed samples, 14 presented PPD content between 1.74 and 3.65% w/w, in disagreement with Legislation. Conclusions: The proposed method can contribute to monitoring of quality and safety of use of these products.Introdução: O p-fenilenodiamina (PPD), amina aromática sensibilizante, vem sendo adicionado ao pó de hena para modificar sua cor natural para preta, aumentando seu tempo de fixação, prática proibida em tinturas para cílios e sobrancelhas. Objetivo: O objetivo deste estudo foi otimizar e validar, em níveis intralaboratoriais, um método analítico por CLAE/UV para identificação e quantificação de PPD em tinturas de hena para cabelos e sobrancelhas. Método: Foi utilizada coluna em fase reversa C8, fase móvel trietanolamina 1% (pH 8,4) e acetonitrila (99:1, v/v), detecção a 280 nm, volume de injeção de 10 μL, fluxo 1,0 mL/min, temperatura da coluna32°C, tempo de corrida 10 min, linearidade 5-45 μg/mL (n = 5) com coeficiente de correlação de 0,9982. Para o teste de Cochran (homocedasticidade), 0,3350 e o Ccrítico (0,3934), com 99% de confiança. Limites de detecção, 1,17 μg/mL e quantificação, 3,54 μg/mL. O coeficiente de variação da repetibilidade 0,12% e na precisão intermediária pelo teste F obteve-se p-valor de 0,283 com 95% de confiança. Resultados: Os resultados da exatidão compreenderam os critérios de aceitação (90%-107%). Das 19 amostras analisadas, 14 apresentaram teor de PPD entre 1,74% a 3,65% p/p, em desacordo com a Legislação. Conclusões: O método proposto poderá contribuir com o monitoramento da qualidade e segurança de uso destes produtos.Instituto Nacional de Controle de Qualidade em Saúde2019-05-31info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion"Peer-reviewed article""Artículo revisado por pares""Artigo avaliado pelos pares"application/pdfapplication/pdfhttps://visaemdebate.incqs.fiocruz.br/index.php/visaemdebate/article/view/126510.22239/2317-269x.01265Health Surveillance under Debate: Society, Science & Technology ; Vol. 7 No. 2 (2019): May; 62-68Vigilancia en Salud en Debate: Sociedad, Ciencia y Tecnología; Vol. 7 Núm. 2 (2019): Puede; 62-68Vigil Sanit Debate, Rio de Janeiro; v. 7 n. 2 (2019): Maio; 62-682317-269Xreponame:Vigilância Sanitária em Debateinstname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZporenghttps://visaemdebate.incqs.fiocruz.br/index.php/visaemdebate/article/view/1265/1013https://visaemdebate.incqs.fiocruz.br/index.php/visaemdebate/article/view/1265/1110Copyright (c) 2019 Vigilância Sanitária em Debate: Sociedade, Ciência & Tecnologia (Health Surveillance under Debate: Society, Science & Technology) – Visa em Debatehttps://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessSanta Barbara, Maria CristinaYano, Helena MiyocoFarias, Fernanda FernandesGasparin, Luiz Fernando OrtizVieira, Edilene AfonsoTrujillo, Luz MarinaPereira Martins, Valeria AdrianaMiyamaru, Lígia LurikoMarkman, Blanca Elena Ortega2023-06-27T15:11:12Zoai:ojs.visaemdebate.incqs.fiocruz.br:article/1265Revistahttps://visaemdebate.incqs.fiocruz.br/index.php/visaemdebatePUBhttps://visaemdebate.incqs.fiocruz.br/index.php/visaemdebate/oaiincqs.visaemdebate@fiocruz.br || gisele.neves@fiocruz.br2317-269X2317-269Xopendoar:2023-06-27T15:11:12Vigilância Sanitária em Debate - Fundação Oswaldo Cruz (FIOCRUZ)false |
dc.title.none.fl_str_mv |
Optimization and intralaboratorial validation of an analytical method by HPLC/UV for identification and quantification of p-phenylenediamine in henna dye hair and eyebrows Otimização e validação intralaboratorial de método analítico por CLAE/UV para identificação e quantificação de p-fenilenodiamina em tinturas de hena para cabelos e sobrancelhas |
title |
Optimization and intralaboratorial validation of an analytical method by HPLC/UV for identification and quantification of p-phenylenediamine in henna dye hair and eyebrows |
spellingShingle |
Optimization and intralaboratorial validation of an analytical method by HPLC/UV for identification and quantification of p-phenylenediamine in henna dye hair and eyebrows Santa Barbara, Maria Cristina p-fenilenodiamina Henna Method Validation PPD Hena Validação de Método |
title_short |
Optimization and intralaboratorial validation of an analytical method by HPLC/UV for identification and quantification of p-phenylenediamine in henna dye hair and eyebrows |
title_full |
Optimization and intralaboratorial validation of an analytical method by HPLC/UV for identification and quantification of p-phenylenediamine in henna dye hair and eyebrows |
title_fullStr |
Optimization and intralaboratorial validation of an analytical method by HPLC/UV for identification and quantification of p-phenylenediamine in henna dye hair and eyebrows |
title_full_unstemmed |
Optimization and intralaboratorial validation of an analytical method by HPLC/UV for identification and quantification of p-phenylenediamine in henna dye hair and eyebrows |
title_sort |
Optimization and intralaboratorial validation of an analytical method by HPLC/UV for identification and quantification of p-phenylenediamine in henna dye hair and eyebrows |
author |
Santa Barbara, Maria Cristina |
author_facet |
Santa Barbara, Maria Cristina Yano, Helena Miyoco Farias, Fernanda Fernandes Gasparin, Luiz Fernando Ortiz Vieira, Edilene Afonso Trujillo, Luz Marina Pereira Martins, Valeria Adriana Miyamaru, Lígia Luriko Markman, Blanca Elena Ortega |
author_role |
author |
author2 |
Yano, Helena Miyoco Farias, Fernanda Fernandes Gasparin, Luiz Fernando Ortiz Vieira, Edilene Afonso Trujillo, Luz Marina Pereira Martins, Valeria Adriana Miyamaru, Lígia Luriko Markman, Blanca Elena Ortega |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Santa Barbara, Maria Cristina Yano, Helena Miyoco Farias, Fernanda Fernandes Gasparin, Luiz Fernando Ortiz Vieira, Edilene Afonso Trujillo, Luz Marina Pereira Martins, Valeria Adriana Miyamaru, Lígia Luriko Markman, Blanca Elena Ortega |
dc.subject.por.fl_str_mv |
p-fenilenodiamina Henna Method Validation PPD Hena Validação de Método |
topic |
p-fenilenodiamina Henna Method Validation PPD Hena Validação de Método |
description |
Introduction: p-Phenylenediamine (PPD), aromatic sensitizing amine, has been added to henna powder to modify its natural color to black, increasing its fixation time, a practice that is prohibited in eyelash and eyebrows dyes. Objective: The objective of this study was to optimize and validate, at an intra-laboratory level, an analytical method by HPLC/UV for identification and quantification of PPD in hen dyes for hair and eyebrows. Method: In the method, C8 reverse phase column, mobile phase 1% triethanolamine (pH 8.4) andacetonitrile (99: 1, v/v), detection 280nm, injection volume: 10uL, flow 1.0 mL/min, column temperature 32°C, run time 10 min, linearity 5-45 μg/mL (n = 5) with correlation coefficient 0.9982, were used. For Cochran test (homoscedasticity): 0.3350 and Critical (0.3934) with 99% confidence. Limits of detection 1.17 μg/mL and quantification 3.54 μg/mL. The coefficient of variation of repeatability was 0.12% and the intermediate precision by F-test yielded p value of 0.283 with 95% of confidence. Accuracy results comprised acceptance criteria of 90%-107%. Results: Of the 19 analyzed samples, 14 presented PPD content between 1.74 and 3.65% w/w, in disagreement with Legislation. Conclusions: The proposed method can contribute to monitoring of quality and safety of use of these products. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-05-31 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion "Peer-reviewed article" "Artículo revisado por pares" "Artigo avaliado pelos pares" |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://visaemdebate.incqs.fiocruz.br/index.php/visaemdebate/article/view/1265 10.22239/2317-269x.01265 |
url |
https://visaemdebate.incqs.fiocruz.br/index.php/visaemdebate/article/view/1265 |
identifier_str_mv |
10.22239/2317-269x.01265 |
dc.language.iso.fl_str_mv |
por eng |
language |
por eng |
dc.relation.none.fl_str_mv |
https://visaemdebate.incqs.fiocruz.br/index.php/visaemdebate/article/view/1265/1013 https://visaemdebate.incqs.fiocruz.br/index.php/visaemdebate/article/view/1265/1110 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Instituto Nacional de Controle de Qualidade em Saúde |
publisher.none.fl_str_mv |
Instituto Nacional de Controle de Qualidade em Saúde |
dc.source.none.fl_str_mv |
Health Surveillance under Debate: Society, Science & Technology ; Vol. 7 No. 2 (2019): May; 62-68 Vigilancia en Salud en Debate: Sociedad, Ciencia y Tecnología; Vol. 7 Núm. 2 (2019): Puede; 62-68 Vigil Sanit Debate, Rio de Janeiro; v. 7 n. 2 (2019): Maio; 62-68 2317-269X reponame:Vigilância Sanitária em Debate instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
instname_str |
Fundação Oswaldo Cruz (FIOCRUZ) |
instacron_str |
FIOCRUZ |
institution |
FIOCRUZ |
reponame_str |
Vigilância Sanitária em Debate |
collection |
Vigilância Sanitária em Debate |
repository.name.fl_str_mv |
Vigilância Sanitária em Debate - Fundação Oswaldo Cruz (FIOCRUZ) |
repository.mail.fl_str_mv |
incqs.visaemdebate@fiocruz.br || gisele.neves@fiocruz.br |
_version_ |
1797042045340090368 |