4D printing of extracellular matrix: pilot project for the development of complex tissues

• Autor(a) principal: Moreira Feijó, Heloisa
• Data de Publicação: 2022
• Outros Autores: Lima de Morais Inocêncio, Mariana, Correia Arruda, Laiz, Benedito Ferreira Freire, Vitor, Eugênio da Silva, Edilson, Duarte de Menezes, Frederico, Stanislaw Michewicz, Jacek, Peixoto da Costa, José Ângelo, Lima Monteiro, Luciana, Leão Silva Lima, Clessio, Barreto Ipiranga, João Victor, de Siqueira Medeiros Filho, Amaury, Eduardo Oliveira Filho, Jorge, Vasconcelos de Albuquerque, Amanda, Lima Monteiro, Leonardo
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Anais da Faculdade de Medicina de Olinda (Online)
• Texto Completo: https://afmo.emnuvens.com.br/afmo/article/view/196
• topic: Bioimpressão; Matriz Extracelular; Medicina Regenerativa; Bioengenharia; Tecidos Suporte; Bioprinting; Extracellular Matrix; Regenerative Medicine; Bioengineering; Scaffolds

Introduction: The shortage of organs and tissues for transplantation to meet the existing demand is one of the great challenges in the medical field. A feasible solution to this problem is the three-dimensional (3D) printing of organs and tissues. The present study presents preliminary results of the first 4D extracellular matrix (ECM) models using a biomaterial. Methods: Experimental, multicenter study carried out at the Simulation and Digital Fabrication Laboratory (SIMUFAB) of the Federal Institute of Pernambuco (IFPE). A 2% sodium alginate solution was used to manufacture the MEC on a Zmorph 3D printer, type FDM. Results: The MEC geometric figure was a square with an area of ​​1cm2 and developed by the Voxelizer software, initially designed in CAD (computer aided design). Next, the biomaterial in gel form was printed with an extrusion speed of 1mm/s and a printing speed of 3mm/s. We succeed in the printing with the biopolymer selected for the manufacture of MEC. There was a uniform deposit of the material during printing, which allowed obtaining the geometric shape designed in CAD. Discussion: This article shows preliminary results of the printing process of a 4D MEC. The achievement of the MEC impression qualifies alginate as one of the main biopolymers to be used in the future phases of this study. Conclusion: The variation of the nozzle area and the extrusion speed have a great influence on the analysis of the final parameters to use the 3D printer.