Efeito das células derivadas da medula óssea no tratamento da insuficiência renal crônica experimental
Autor(a) principal: | |
---|---|
Data de Publicação: | 2011 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da FAMERP |
Texto Completo: | http://bdtd.famerp.br/handle/tede/104 |
Resumo: | Chronic renal failure (CRF) is characterized by progressive and irreversible loss of renal function and its treatment generates significant public spending for maintenance and care of patients on dialysis. Stem cell (SC) therapy, in its potential for treatment of chronic diseases, may be a promising strategy for repairing the damage from or slowing the progression of CRF. There are questions about cell type, quantity of cells, method and ideal place for deployment of SC and the role it plays in the repair of renal parenchyma. Objective: 1) To evaluate the effect of infusion of bone marrow derived cells (BMDC) in the treatment of experimental CRF; 2) Evaluate the combined effect of SC and biomaterial (BM) in the progression of CRF and study the effect of this therapy in different stages of CRF; 3) Evaluate the development of techniques for isolation and cultivation of human umbilical cord blood (HUCB) mesenchymal cells Methods: Article 1: We used the 5/6 mass reduction model to induce experimental CRF. Kidney function was measured at the beginning of the experiment and 60 and 120 days after the surgery; Article 2: Animals were subdivided as to the amount of renal parenchyma injured (5/6 or 2/3), the use of BM as a scaffold to cell implantation, and cell type used (mononuclear or mesenchymal cells). Renal function was evaluated on days 0, 45, and 90 after surgery. Histological and immunohistochemical analyses were done in all groups at the end of the study; Article 3: Ten samples of HUCB were used and two different procedures for cultivation of mesenchymal stem cells (MSC) were tested: without Ficoll-Paque density gradient, to obtain nucleated cells; with Ficoll-Paque density gradient, for obtaining mononuclear cells. Results: Article 1: CRF progression analysis showed that treatment with BMDC significantly reduced the rate of decline of creatinine clearance (Clcr) when compared with the control group; Article 2:Treated animals showed significantly lower increases in serum creatinine and 24 hour proteinuria, and higher increases in Clcr after 90 days when compared to control animals in both models of CRF; Article 3: The MSC in culture from the method without Ficoll-Paque density gradient maintained growth forming confluent cell foci. Conclusions: Article 1: Progression of CRF can be delayed by injection of BMDC in the renal parenchyma; Article 2: a) Use of SC combined with BM can be an alternative way to administer BMDC; b) Cell therapy seems to be most effective when administered in less severe stages of CRF; Article 3: Nucleated cells without using Ficoll-Paque density gradient showed more efficiency in the cultivation of MSC from HUCB when compared with the procedure employing Ficoll-Paque density gradient. |
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Abbud Filho, MarioCPF:74478478872http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4781535Y4Braile, Domingo MarcolinoCPF:01172786887http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4767966J3&dataRevisao=nullGoloni-bertollo, Eny MariaCPF:04643634898http://lattes.cnpq.br/9176636696202692Lima-oliveira, Ana Paula Marques deCPF:00000000168http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4792585P6Goissis, GilbertoCPF:00000000072http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4727294D8CPF:21304383857http://lattes.cnpq.br/0925801342254577Caldas, Heloisa Cristina2016-01-26T12:51:30Z2012-02-092011-07-26CALDAS, Heloisa Cristina. Efeito das células derivadas da medula óssea no tratamento da insuficiência renal crônica experimental. 2011. 81 f. Tese (Doutorado em Medicina Interna; Medicina e Ciências Correlatas) - Faculdade de Medicina de São José do Rio Preto, São José do Rio Preto, 2011.http://bdtd.famerp.br/handle/tede/104Chronic renal failure (CRF) is characterized by progressive and irreversible loss of renal function and its treatment generates significant public spending for maintenance and care of patients on dialysis. Stem cell (SC) therapy, in its potential for treatment of chronic diseases, may be a promising strategy for repairing the damage from or slowing the progression of CRF. There are questions about cell type, quantity of cells, method and ideal place for deployment of SC and the role it plays in the repair of renal parenchyma. Objective: 1) To evaluate the effect of infusion of bone marrow derived cells (BMDC) in the treatment of experimental CRF; 2) Evaluate the combined effect of SC and biomaterial (BM) in the progression of CRF and study the effect of this therapy in different stages of CRF; 3) Evaluate the development of techniques for isolation and cultivation of human umbilical cord blood (HUCB) mesenchymal cells Methods: Article 1: We used the 5/6 mass reduction model to induce experimental CRF. Kidney function was measured at the beginning of the experiment and 60 and 120 days after the surgery; Article 2: Animals were subdivided as to the amount of renal parenchyma injured (5/6 or 2/3), the use of BM as a scaffold to cell implantation, and cell type used (mononuclear or mesenchymal cells). Renal function was evaluated on days 0, 45, and 90 after surgery. Histological and immunohistochemical analyses were done in all groups at the end of the study; Article 3: Ten samples of HUCB were used and two different procedures for cultivation of mesenchymal stem cells (MSC) were tested: without Ficoll-Paque density gradient, to obtain nucleated cells; with Ficoll-Paque density gradient, for obtaining mononuclear cells. Results: Article 1: CRF progression analysis showed that treatment with BMDC significantly reduced the rate of decline of creatinine clearance (Clcr) when compared with the control group; Article 2:Treated animals showed significantly lower increases in serum creatinine and 24 hour proteinuria, and higher increases in Clcr after 90 days when compared to control animals in both models of CRF; Article 3: The MSC in culture from the method without Ficoll-Paque density gradient maintained growth forming confluent cell foci. Conclusions: Article 1: Progression of CRF can be delayed by injection of BMDC in the renal parenchyma; Article 2: a) Use of SC combined with BM can be an alternative way to administer BMDC; b) Cell therapy seems to be most effective when administered in less severe stages of CRF; Article 3: Nucleated cells without using Ficoll-Paque density gradient showed more efficiency in the cultivation of MSC from HUCB when compared with the procedure employing Ficoll-Paque density gradient.A insuficiência renal crônica (IRC) é caracterizada pela perda progressiva e irreversível da função renal e seu tratamento gera um gasto público significativo para manutenção de pacientes em tratamento dialítico. A terapia com células-tronco (CT), pelo seu potencial de tratamento das doenças crônicas, pode ser uma estratégia promissora para reparar ou retardar a progressão da IRC. Existem dúvidas sobre o tipo celular, a quantidade de células, o método e local ideal para implantação das CT e o papel por elas desempenhado na reparação do parênquima renal. Objetivos: 1) avaliar o efeito da infusão de células derivadas da medula óssea (CDMO) no tratamento da IRC experimental; 2) avaliar o efeito combinado das CT e biomaterial (BM) na progressão da IRC e estudar o efeito dessa terapia em diferentes estágios da IRC; 3) Avaliar o desenvolvimento de técnicas de isolamento e cultivo de células mesenquimais do sangue de cordão umbilical humano (SCU). Métodos: artigo 1: usamos o modelo de redução de massa 5/6 para induzir a IRC experimental. Função renal foi medida no início do experimento e 60 e 120 dias depois da cirurgia; artigo 2: animais foram subdivididos conforme a quantidade de parênquima renal lesado (5/6 ou 2/3), o uso de BM como arcabouço para o implante celular e o tipo de células utilizado (célula mononuclear ou mesenquimal). A função renal foi avaliada nos dias 0, 45 e 90 após cirurgia. Análise histológica e imunohistoquimica foram realizadas em todos os grupos ao final do estudo; artigo 3: Foram utilizadas dez amostras de SCU e testados dois diferentes procedimentos para cultivo de células-tronco mesenquimal (CTM): sem gradiente de densidade Ficoll-Paque, para obtenção de células nucleadas; por gradiente de densidade Ficoll-Paque, para obtenção de células mononucleares. Resultados: artigo 1: Análises da progressão da IRC mostraram que o tratamento com CDMO reduziu significativamente a taxa de declínio do clearance ( Clcr) quando comparados com o grupo controle; artigo 2: animais tratados apresentaram aumentos significativamente menores de creatinina sérica, proteinúria e Clcr maiores após 90 dias, quando comparado aos animais controles em ambos os modelos de IRC; artigo 3: as CTM em cultura provenientes do método sem gradiente de densidade Ficoll- Paque mantiveram o crescimento formando focos confluentes de células. Conclusões: artigo 1: a progressão da IRC pode ser retardada pela injeção de CDMO no parênquima renal; artigo 2: a) utilização da CT combinada com o BM pode ser uma via alternativa para administrar a CTMO; b) terapia celular parece ser mais eficaz quando administrada em estágios menos graves da IRC; artigo 3: As células nucleadas sem uso do gradiente de densidade Ficoll-Paque mostraram mais eficiente para o cultivo de CTM do SCU quando comparado ao procedimento com gradiente de densidade Ficoll-Paque.Made available in DSpace on 2016-01-26T12:51:30Z (GMT). No. of bitstreams: 1 heloisacaldas_tese.pdf: 13961449 bytes, checksum: adc041656d053c466bd2f0851119388c (MD5) Previous issue date: 2011-07-26application/pdfporFaculdade de Medicina de São José do Rio PretoPrograma de Pós-Graduação em Ciências da Saúde::123123::600FAMERPBRMedicina Interna; Medicina e Ciências Correlatas::123123::600Cellular TherapyStem cellsTissue engineeringChronic renal failureUmbilical cord bloodTerapia celularCélulas-troncoEngenharia tecidualInsuficiência renal crônicaSangue de cordão umbilicalCNPQ::CIENCIAS DA SAUDE::MEDICINA::CLINICA MEDICA::NEFROLOGIA::123123::600Efeito das células derivadas da medula óssea no tratamento da insuficiência renal crônica experimentalinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da FAMERPinstname:Faculdade de Medicina de São José do Rio Preto (FAMERP)instacron:FAMERPORIGINALheloisacaldas_tese.pdfapplication/pdf13961449adc041656d053c466bd2f0851119388cMD51http://bdtd.famerp.br/bitstream/tede/104/1/heloisacaldas_tese.pdftede/1042019-02-04 11:06:05.076oai:localhost:tede/104Biblioteca Digital de Teses e Dissertaçõeshttp://bdtd.famerp.br/PUBhttps://bdtd.famerp.br/oai/requestsbdc@famerp.br||joao.junior@famerp.bropendoar:47112019-02-04T13:06:05Biblioteca Digital de Teses e Dissertações da FAMERP - Faculdade de Medicina de São José do Rio Preto (FAMERP)false |
dc.title.por.fl_str_mv |
Efeito das células derivadas da medula óssea no tratamento da insuficiência renal crônica experimental |
title |
Efeito das células derivadas da medula óssea no tratamento da insuficiência renal crônica experimental |
spellingShingle |
Efeito das células derivadas da medula óssea no tratamento da insuficiência renal crônica experimental Caldas, Heloisa Cristina Cellular Therapy Stem cells Tissue engineering Chronic renal failure Umbilical cord blood Terapia celular Células-tronco Engenharia tecidual Insuficiência renal crônica Sangue de cordão umbilical CNPQ::CIENCIAS DA SAUDE::MEDICINA::CLINICA MEDICA::NEFROLOGIA::123123::600 |
title_short |
Efeito das células derivadas da medula óssea no tratamento da insuficiência renal crônica experimental |
title_full |
Efeito das células derivadas da medula óssea no tratamento da insuficiência renal crônica experimental |
title_fullStr |
Efeito das células derivadas da medula óssea no tratamento da insuficiência renal crônica experimental |
title_full_unstemmed |
Efeito das células derivadas da medula óssea no tratamento da insuficiência renal crônica experimental |
title_sort |
Efeito das células derivadas da medula óssea no tratamento da insuficiência renal crônica experimental |
author |
Caldas, Heloisa Cristina |
author_facet |
Caldas, Heloisa Cristina |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Abbud Filho, Mario |
dc.contributor.advisor1ID.fl_str_mv |
CPF:74478478872 |
dc.contributor.advisor1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4781535Y4 |
dc.contributor.referee1.fl_str_mv |
Braile, Domingo Marcolino |
dc.contributor.referee1ID.fl_str_mv |
CPF:01172786887 |
dc.contributor.referee1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4767966J3&dataRevisao=null |
dc.contributor.referee2.fl_str_mv |
Goloni-bertollo, Eny Maria |
dc.contributor.referee2ID.fl_str_mv |
CPF:04643634898 |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/9176636696202692 |
dc.contributor.referee3.fl_str_mv |
Lima-oliveira, Ana Paula Marques de |
dc.contributor.referee3ID.fl_str_mv |
CPF:00000000168 |
dc.contributor.referee3Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4792585P6 |
dc.contributor.referee4.fl_str_mv |
Goissis, Gilberto |
dc.contributor.referee4ID.fl_str_mv |
CPF:00000000072 |
dc.contributor.referee4Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4727294D8 |
dc.contributor.authorID.fl_str_mv |
CPF:21304383857 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/0925801342254577 |
dc.contributor.author.fl_str_mv |
Caldas, Heloisa Cristina |
contributor_str_mv |
Abbud Filho, Mario Braile, Domingo Marcolino Goloni-bertollo, Eny Maria Lima-oliveira, Ana Paula Marques de Goissis, Gilberto |
dc.subject.eng.fl_str_mv |
Cellular Therapy Stem cells Tissue engineering Chronic renal failure Umbilical cord blood |
topic |
Cellular Therapy Stem cells Tissue engineering Chronic renal failure Umbilical cord blood Terapia celular Células-tronco Engenharia tecidual Insuficiência renal crônica Sangue de cordão umbilical CNPQ::CIENCIAS DA SAUDE::MEDICINA::CLINICA MEDICA::NEFROLOGIA::123123::600 |
dc.subject.por.fl_str_mv |
Terapia celular Células-tronco Engenharia tecidual Insuficiência renal crônica Sangue de cordão umbilical |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS DA SAUDE::MEDICINA::CLINICA MEDICA::NEFROLOGIA::123123::600 |
description |
Chronic renal failure (CRF) is characterized by progressive and irreversible loss of renal function and its treatment generates significant public spending for maintenance and care of patients on dialysis. Stem cell (SC) therapy, in its potential for treatment of chronic diseases, may be a promising strategy for repairing the damage from or slowing the progression of CRF. There are questions about cell type, quantity of cells, method and ideal place for deployment of SC and the role it plays in the repair of renal parenchyma. Objective: 1) To evaluate the effect of infusion of bone marrow derived cells (BMDC) in the treatment of experimental CRF; 2) Evaluate the combined effect of SC and biomaterial (BM) in the progression of CRF and study the effect of this therapy in different stages of CRF; 3) Evaluate the development of techniques for isolation and cultivation of human umbilical cord blood (HUCB) mesenchymal cells Methods: Article 1: We used the 5/6 mass reduction model to induce experimental CRF. Kidney function was measured at the beginning of the experiment and 60 and 120 days after the surgery; Article 2: Animals were subdivided as to the amount of renal parenchyma injured (5/6 or 2/3), the use of BM as a scaffold to cell implantation, and cell type used (mononuclear or mesenchymal cells). Renal function was evaluated on days 0, 45, and 90 after surgery. Histological and immunohistochemical analyses were done in all groups at the end of the study; Article 3: Ten samples of HUCB were used and two different procedures for cultivation of mesenchymal stem cells (MSC) were tested: without Ficoll-Paque density gradient, to obtain nucleated cells; with Ficoll-Paque density gradient, for obtaining mononuclear cells. Results: Article 1: CRF progression analysis showed that treatment with BMDC significantly reduced the rate of decline of creatinine clearance (Clcr) when compared with the control group; Article 2:Treated animals showed significantly lower increases in serum creatinine and 24 hour proteinuria, and higher increases in Clcr after 90 days when compared to control animals in both models of CRF; Article 3: The MSC in culture from the method without Ficoll-Paque density gradient maintained growth forming confluent cell foci. Conclusions: Article 1: Progression of CRF can be delayed by injection of BMDC in the renal parenchyma; Article 2: a) Use of SC combined with BM can be an alternative way to administer BMDC; b) Cell therapy seems to be most effective when administered in less severe stages of CRF; Article 3: Nucleated cells without using Ficoll-Paque density gradient showed more efficiency in the cultivation of MSC from HUCB when compared with the procedure employing Ficoll-Paque density gradient. |
publishDate |
2011 |
dc.date.issued.fl_str_mv |
2011-07-26 |
dc.date.available.fl_str_mv |
2012-02-09 |
dc.date.accessioned.fl_str_mv |
2016-01-26T12:51:30Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/doctoralThesis |
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doctoralThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
CALDAS, Heloisa Cristina. Efeito das células derivadas da medula óssea no tratamento da insuficiência renal crônica experimental. 2011. 81 f. Tese (Doutorado em Medicina Interna; Medicina e Ciências Correlatas) - Faculdade de Medicina de São José do Rio Preto, São José do Rio Preto, 2011. |
dc.identifier.uri.fl_str_mv |
http://bdtd.famerp.br/handle/tede/104 |
identifier_str_mv |
CALDAS, Heloisa Cristina. Efeito das células derivadas da medula óssea no tratamento da insuficiência renal crônica experimental. 2011. 81 f. Tese (Doutorado em Medicina Interna; Medicina e Ciências Correlatas) - Faculdade de Medicina de São José do Rio Preto, São José do Rio Preto, 2011. |
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http://bdtd.famerp.br/handle/tede/104 |
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por |
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Faculdade de Medicina de São José do Rio Preto |
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BR |
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Medicina Interna; Medicina e Ciências Correlatas::123123::600 |
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Faculdade de Medicina de São José do Rio Preto |
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