Effect of chitosan on the mycelial growth of Fusarium oxysporum, Rhizoctonia solani and Trichoderma spp.

Detalhes bibliográficos
Autor(a) principal: Siqueira, Amanda Keller
Data de Publicação: 2020
Outros Autores: Levandoski Junior, Osmar da Rocha, Bertan, Gabriela Aparecida Pereira, de Brito, Jeislane Camila Sampietro Klack, da Silva, Vanessa Gomes
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Atas de Saúde Ambiental
Texto Completo: https://revistaseletronicas.fmu.br/index.php/ASA/article/view/2107
Resumo: Fungicides, often used in conventional farming systems, can harm humans, animals and damage the environment. Chitosan, which is extracted from the crustacean shell, has shown to be a harmless and cheap alternative to fungicides. This study aimed to test the inhibitory effect of chitosan on the mycelial growth of Fusarium oxysporum, Rhizoctonia solani and Trichoderma spp. Different concentrations of chitosan (0, 1, 2, 4, 8 and 16%) were supplemented to 1% acetic acid Potato Dextrose Agar (PDA). The fungi were inoculated in the center of the plates, incubated at 25 °C, with or without a 12-hour photoperiod. Daily measurements were performed in two transverse diameters until the mycelium reached the edges of the Petri dish. Fusarium oxysporum mycelial growth was significantly inhibited by chitosan at concentrations 1, 2, 4, and 8%, on the 4th, 5th and 6th evaluation days, in a 12-hour photoperiod. However, for Rhizoctonia solani, there was no reduction on mycelial growth. Trichoderma spp. presented the greatest mycelial reduction, in both light schemes, in all the concentrations evaluated, on days one, two and three. The results indicate that the mycelial growth of Fusarium oxysporum and Trichoderma spp. were inhibited by chitosan. Although, the combined use of chitosan and Trichoderma spp. as biocontrol agents of phytopathogens is not indicated.
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spelling Effect of chitosan on the mycelial growth of Fusarium oxysporum, Rhizoctonia solani and Trichoderma spp.EFEITO DA QUITOSANA NO CRESCIMENTO MICELIAL DE FUSARIUM OXYSPORUM, RHIZOCTONIA SOLANI E TRICHODERMA SPP.biofungicidecontrolfungishrimpbiofungicidacamarãocontrolefungos.Fungicides, often used in conventional farming systems, can harm humans, animals and damage the environment. Chitosan, which is extracted from the crustacean shell, has shown to be a harmless and cheap alternative to fungicides. This study aimed to test the inhibitory effect of chitosan on the mycelial growth of Fusarium oxysporum, Rhizoctonia solani and Trichoderma spp. Different concentrations of chitosan (0, 1, 2, 4, 8 and 16%) were supplemented to 1% acetic acid Potato Dextrose Agar (PDA). The fungi were inoculated in the center of the plates, incubated at 25 °C, with or without a 12-hour photoperiod. Daily measurements were performed in two transverse diameters until the mycelium reached the edges of the Petri dish. Fusarium oxysporum mycelial growth was significantly inhibited by chitosan at concentrations 1, 2, 4, and 8%, on the 4th, 5th and 6th evaluation days, in a 12-hour photoperiod. However, for Rhizoctonia solani, there was no reduction on mycelial growth. Trichoderma spp. presented the greatest mycelial reduction, in both light schemes, in all the concentrations evaluated, on days one, two and three. The results indicate that the mycelial growth of Fusarium oxysporum and Trichoderma spp. were inhibited by chitosan. Although, the combined use of chitosan and Trichoderma spp. as biocontrol agents of phytopathogens is not indicated.Os fungicidas, frequentemente utilizados em sistemas agrí­colas convencionais, podem prejudicar seres humanos, animais e danificar o meio ambiente. A quitosana, extraí­da da casca do crustáceo, tem se mostrado uma alternativa inofensiva e barata aos fungicidas. Este trabalho teve como objetivo testar o efeito inibitório da quitosana sobre o crescimento micelial de Fusarium oxysporum, Rhizoctonia solani e Trichoderma spp. Diferentes concentrações de quitosana (0, 1, 2, 4, 8 e 16%) foram adicionadas em Batata Dextrose Agar (BDA) preparado em 1% de ácido acético. Os fungos foram inoculados no centro das placas, incubados a 25 °C, com ou sem fotoperí­odo de 12 horas. Medições diárias foram realizadas em dois diâmetros transversais até o micélio atingir as bordas da placa de Petri. O crescimento micelial de Fusarium oxysporum foi significativamente inibido pela quitosana nas concentrações 1, 2, 4 e 8%, nos dias 4, 5 e 6 de avaliação, em fotoperí­odo de 12 horas. No entanto, para Rhizoctonia solani não houve redução no crescimento micelial. Trichoderma spp. apresentou a maior redução micelial, em ambos os esquemas de luz, em todas as concentrações avaliadas, nos dias um, dois e três. Os resultados indicam que o crescimento micelial de Fusarium oxysporum e Trichoderma spp. foram inibidos pela quitosana. Assim, o uso combinado de quitosana e Trichoderma spp. como agentes de biocontrole de fitopatógenos não é indicado.Atas de Saúde Ambiental - ASA (ISSN 2357-7614)2020-03-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revistaseletronicas.fmu.br/index.php/ASA/article/view/2107Atas de Saúde Ambiental - ASA (ISSN 2357-7614); v. 8 (2020); 342357-7614reponame:Atas de Saúde Ambientalinstname:Faculdades Metropolitanas Unidas (FMU)instacron:FMUenghttps://revistaseletronicas.fmu.br/index.php/ASA/article/view/2107/1547Copyright (c) 2020 Amanda Keller Siqueira, Osmar da Rocha Levandoski Junior, Gabriela Aparecida Pereira Bertan, Jeislane Camila Sampietro Klack de Brito, Vanessa Gomes da Silvainfo:eu-repo/semantics/openAccessSiqueira, Amanda KellerLevandoski Junior, Osmar da RochaBertan, Gabriela Aparecida Pereirade Brito, Jeislane Camila Sampietro Klackda Silva, Vanessa Gomes2020-03-25T17:04:29Zoai:ojs.fmu.br:article/2107Revistahttps://revistaseletronicas.fmu.br/index.php/ASA/indexPRIhttps://revistaseletronicas.fmu.br/index.php/ASA/oai||asa.revista@gmail.com2357-76142357-7614opendoar:2020-03-25T17:04:29Atas de Saúde Ambiental - Faculdades Metropolitanas Unidas (FMU)false
dc.title.none.fl_str_mv Effect of chitosan on the mycelial growth of Fusarium oxysporum, Rhizoctonia solani and Trichoderma spp.
EFEITO DA QUITOSANA NO CRESCIMENTO MICELIAL DE FUSARIUM OXYSPORUM, RHIZOCTONIA SOLANI E TRICHODERMA SPP.
title Effect of chitosan on the mycelial growth of Fusarium oxysporum, Rhizoctonia solani and Trichoderma spp.
spellingShingle Effect of chitosan on the mycelial growth of Fusarium oxysporum, Rhizoctonia solani and Trichoderma spp.
Siqueira, Amanda Keller
biofungicide
control
fungi
shrimp
biofungicida
camarão
controle
fungos.
title_short Effect of chitosan on the mycelial growth of Fusarium oxysporum, Rhizoctonia solani and Trichoderma spp.
title_full Effect of chitosan on the mycelial growth of Fusarium oxysporum, Rhizoctonia solani and Trichoderma spp.
title_fullStr Effect of chitosan on the mycelial growth of Fusarium oxysporum, Rhizoctonia solani and Trichoderma spp.
title_full_unstemmed Effect of chitosan on the mycelial growth of Fusarium oxysporum, Rhizoctonia solani and Trichoderma spp.
title_sort Effect of chitosan on the mycelial growth of Fusarium oxysporum, Rhizoctonia solani and Trichoderma spp.
author Siqueira, Amanda Keller
author_facet Siqueira, Amanda Keller
Levandoski Junior, Osmar da Rocha
Bertan, Gabriela Aparecida Pereira
de Brito, Jeislane Camila Sampietro Klack
da Silva, Vanessa Gomes
author_role author
author2 Levandoski Junior, Osmar da Rocha
Bertan, Gabriela Aparecida Pereira
de Brito, Jeislane Camila Sampietro Klack
da Silva, Vanessa Gomes
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Siqueira, Amanda Keller
Levandoski Junior, Osmar da Rocha
Bertan, Gabriela Aparecida Pereira
de Brito, Jeislane Camila Sampietro Klack
da Silva, Vanessa Gomes
dc.subject.por.fl_str_mv biofungicide
control
fungi
shrimp
biofungicida
camarão
controle
fungos.
topic biofungicide
control
fungi
shrimp
biofungicida
camarão
controle
fungos.
description Fungicides, often used in conventional farming systems, can harm humans, animals and damage the environment. Chitosan, which is extracted from the crustacean shell, has shown to be a harmless and cheap alternative to fungicides. This study aimed to test the inhibitory effect of chitosan on the mycelial growth of Fusarium oxysporum, Rhizoctonia solani and Trichoderma spp. Different concentrations of chitosan (0, 1, 2, 4, 8 and 16%) were supplemented to 1% acetic acid Potato Dextrose Agar (PDA). The fungi were inoculated in the center of the plates, incubated at 25 °C, with or without a 12-hour photoperiod. Daily measurements were performed in two transverse diameters until the mycelium reached the edges of the Petri dish. Fusarium oxysporum mycelial growth was significantly inhibited by chitosan at concentrations 1, 2, 4, and 8%, on the 4th, 5th and 6th evaluation days, in a 12-hour photoperiod. However, for Rhizoctonia solani, there was no reduction on mycelial growth. Trichoderma spp. presented the greatest mycelial reduction, in both light schemes, in all the concentrations evaluated, on days one, two and three. The results indicate that the mycelial growth of Fusarium oxysporum and Trichoderma spp. were inhibited by chitosan. Although, the combined use of chitosan and Trichoderma spp. as biocontrol agents of phytopathogens is not indicated.
publishDate 2020
dc.date.none.fl_str_mv 2020-03-12
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revistaseletronicas.fmu.br/index.php/ASA/article/view/2107
url https://revistaseletronicas.fmu.br/index.php/ASA/article/view/2107
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://revistaseletronicas.fmu.br/index.php/ASA/article/view/2107/1547
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Atas de Saúde Ambiental - ASA (ISSN 2357-7614)
publisher.none.fl_str_mv Atas de Saúde Ambiental - ASA (ISSN 2357-7614)
dc.source.none.fl_str_mv Atas de Saúde Ambiental - ASA (ISSN 2357-7614); v. 8 (2020); 34
2357-7614
reponame:Atas de Saúde Ambiental
instname:Faculdades Metropolitanas Unidas (FMU)
instacron:FMU
instname_str Faculdades Metropolitanas Unidas (FMU)
instacron_str FMU
institution FMU
reponame_str Atas de Saúde Ambiental
collection Atas de Saúde Ambiental
repository.name.fl_str_mv Atas de Saúde Ambiental - Faculdades Metropolitanas Unidas (FMU)
repository.mail.fl_str_mv ||asa.revista@gmail.com
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