Porcine circovirus 2 detection in feces and sty samples from an unvaccinated farm using PCR

• Autor(a) principal: Briani, Alessandra Barone
• Data de Publicação: 2016
• Outros Autores: Moraes, Carlos Henrique Quarello de, Alvarenga, Elmar de Azevedo, Silveira, Fernanda Rodrigues da, Quintanilha, Suzana Cristina, Baldissera Jr., Flavio A., Castro, Alessandra Marnie Martins Gomes de
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Atas de Saúde Ambiental
• Texto Completo: https://revistaseletronicas.fmu.br/index.php/ASA/article/view/946
• topic: circovirus; PCV2; PCR; porcine; circovirus; PCV2; PCR; suí­nos.

The circovirosis caused by porcine circovirus 2 (PCV2) is responsible for generating economic losses in swine production in Brazil. Infected animals can develop post weaning multisystemic wasting syndrome (PMWS), reproductive disorders and respiratory diseases. An important subclinical manifestation leads to arrested development. Feces are the main form of contamination. Vaccination is the most effective way to decrease the viral circulation, because it minimizes viral shedding in feces. Unfortunately, the high cost prevents the use of the vaccine for many producers. The objective of this study was to detect PCV2 in stool samples collected from piglets and from the sty of a pig farm, unvaccinated for the agent. A total of 16 samples were collected using swabs, six from the rectum of animals and 10 from the sty. DNA extraction was performed using the Qiagen QIAamp kit, followed by PCR amplification. The amplicons were checked on agarose gel electrophoresis. Our results showed that 87.5% of the samples were positive for PCV2 (14/16), 83.3% of tested animals were infected (5/6) as well as 90% of the sty samples (9/10), indicating a high spread of the virus in the environment. These results were similar to those described in the literature for farms that also did not use vaccination to control PCV2 infection.