The Effects of Experimental Intratympanic Steroid Administration on Organ of Corti Type 1 Spiral Ganglion
Autor(a) principal: | |
---|---|
Data de Publicação: | 2018 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | International Archives of Otorhinolaryngology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1809-48642018000200171 |
Resumo: | Abstract Introduction It is unclear how effective is the intratympanic (IT) steroid treatment on organ of Corti type 1 spiral ganglion, its optimal dosage and frequency of administration. The effect of dexamethasone on cochlear functions in individuals with a normal hearing ability is also unknown. Objective The aim of this study was to evaluate, at the electrophysiological and ultrastructural levels, the effect of IT dexamethasone administration in guinea pigs with normal hearing on organ of Corti type 1 spiral ganglion. Methods A total of 20 guinea pigs (n = 40 ears) whose hearing was detected to be normal by electrophysiological tests were included in the study and randomly divided into 6 groups. Four groups were considered study groups, while 2 groups were considered control groups. Dexamethasone was administered intratympanically in doses of 2 mg/mL and 4 mg/mL in the guinea pigs in the study groups. The animals in the control groups received physiological saline in equal doses as the study groups. All interventions were performed under general anesthesia, and the electrophysiological tests were repeated following the IT injections. Results No statistically significant differences were found among the groups when the IT injections were evaluated in terms of the electrophysiological measurements (p > 0.05). The ultrastructural evaluation showed a cellular mitochondrial increase in the spiral ganglions of the cochlea in the groups in which dexamethasone was administered in a dose of 4 mg/mL. Conclusion According to the findings of this study, it can be suggested that the IT injection of dexamethasone is safe, and when applied in a dose of 4mg/mL, it increases metabolic activity at the cellular level. |
id |
FORL-1_74b54333e4112dbad193b288f0b5fab2 |
---|---|
oai_identifier_str |
oai:scielo:S1809-48642018000200171 |
network_acronym_str |
FORL-1 |
network_name_str |
International Archives of Otorhinolaryngology |
repository_id_str |
|
spelling |
The Effects of Experimental Intratympanic Steroid Administration on Organ of Corti Type 1 Spiral Ganglionintratympanicspiral ganglionguinea pigmicroscopydexamethasoneAbstract Introduction It is unclear how effective is the intratympanic (IT) steroid treatment on organ of Corti type 1 spiral ganglion, its optimal dosage and frequency of administration. The effect of dexamethasone on cochlear functions in individuals with a normal hearing ability is also unknown. Objective The aim of this study was to evaluate, at the electrophysiological and ultrastructural levels, the effect of IT dexamethasone administration in guinea pigs with normal hearing on organ of Corti type 1 spiral ganglion. Methods A total of 20 guinea pigs (n = 40 ears) whose hearing was detected to be normal by electrophysiological tests were included in the study and randomly divided into 6 groups. Four groups were considered study groups, while 2 groups were considered control groups. Dexamethasone was administered intratympanically in doses of 2 mg/mL and 4 mg/mL in the guinea pigs in the study groups. The animals in the control groups received physiological saline in equal doses as the study groups. All interventions were performed under general anesthesia, and the electrophysiological tests were repeated following the IT injections. Results No statistically significant differences were found among the groups when the IT injections were evaluated in terms of the electrophysiological measurements (p > 0.05). The ultrastructural evaluation showed a cellular mitochondrial increase in the spiral ganglions of the cochlea in the groups in which dexamethasone was administered in a dose of 4 mg/mL. Conclusion According to the findings of this study, it can be suggested that the IT injection of dexamethasone is safe, and when applied in a dose of 4mg/mL, it increases metabolic activity at the cellular level.Fundação Otorrinolaringologia2018-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1809-48642018000200171International Archives of Otorhinolaryngology v.22 n.2 2018reponame:International Archives of Otorhinolaryngologyinstname:Fundação Otorrinolaringologia (FORL)instacron:FORL10.1055/s-0037-1604067info:eu-repo/semantics/openAccessTopcuoglu,TurgayKocyıgıt,MuratBulut,ErdoganOrtekın,Safiye G.Kanter,MehmetYagız,Recepeng2018-06-28T00:00:00Zoai:scielo:S1809-48642018000200171Revistahttps://www.scielo.br/j/iao/https://old.scielo.br/oai/scielo-oai.php||iaorl@iaorl.org||archives@internationalarchivesent.org||arquivos@forl.org.br1809-48641809-4864opendoar:2018-06-28T00:00International Archives of Otorhinolaryngology - Fundação Otorrinolaringologia (FORL)false |
dc.title.none.fl_str_mv |
The Effects of Experimental Intratympanic Steroid Administration on Organ of Corti Type 1 Spiral Ganglion |
title |
The Effects of Experimental Intratympanic Steroid Administration on Organ of Corti Type 1 Spiral Ganglion |
spellingShingle |
The Effects of Experimental Intratympanic Steroid Administration on Organ of Corti Type 1 Spiral Ganglion Topcuoglu,Turgay intratympanic spiral ganglion guinea pig microscopy dexamethasone |
title_short |
The Effects of Experimental Intratympanic Steroid Administration on Organ of Corti Type 1 Spiral Ganglion |
title_full |
The Effects of Experimental Intratympanic Steroid Administration on Organ of Corti Type 1 Spiral Ganglion |
title_fullStr |
The Effects of Experimental Intratympanic Steroid Administration on Organ of Corti Type 1 Spiral Ganglion |
title_full_unstemmed |
The Effects of Experimental Intratympanic Steroid Administration on Organ of Corti Type 1 Spiral Ganglion |
title_sort |
The Effects of Experimental Intratympanic Steroid Administration on Organ of Corti Type 1 Spiral Ganglion |
author |
Topcuoglu,Turgay |
author_facet |
Topcuoglu,Turgay Kocyıgıt,Murat Bulut,Erdogan Ortekın,Safiye G. Kanter,Mehmet Yagız,Recep |
author_role |
author |
author2 |
Kocyıgıt,Murat Bulut,Erdogan Ortekın,Safiye G. Kanter,Mehmet Yagız,Recep |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Topcuoglu,Turgay Kocyıgıt,Murat Bulut,Erdogan Ortekın,Safiye G. Kanter,Mehmet Yagız,Recep |
dc.subject.por.fl_str_mv |
intratympanic spiral ganglion guinea pig microscopy dexamethasone |
topic |
intratympanic spiral ganglion guinea pig microscopy dexamethasone |
description |
Abstract Introduction It is unclear how effective is the intratympanic (IT) steroid treatment on organ of Corti type 1 spiral ganglion, its optimal dosage and frequency of administration. The effect of dexamethasone on cochlear functions in individuals with a normal hearing ability is also unknown. Objective The aim of this study was to evaluate, at the electrophysiological and ultrastructural levels, the effect of IT dexamethasone administration in guinea pigs with normal hearing on organ of Corti type 1 spiral ganglion. Methods A total of 20 guinea pigs (n = 40 ears) whose hearing was detected to be normal by electrophysiological tests were included in the study and randomly divided into 6 groups. Four groups were considered study groups, while 2 groups were considered control groups. Dexamethasone was administered intratympanically in doses of 2 mg/mL and 4 mg/mL in the guinea pigs in the study groups. The animals in the control groups received physiological saline in equal doses as the study groups. All interventions were performed under general anesthesia, and the electrophysiological tests were repeated following the IT injections. Results No statistically significant differences were found among the groups when the IT injections were evaluated in terms of the electrophysiological measurements (p > 0.05). The ultrastructural evaluation showed a cellular mitochondrial increase in the spiral ganglions of the cochlea in the groups in which dexamethasone was administered in a dose of 4 mg/mL. Conclusion According to the findings of this study, it can be suggested that the IT injection of dexamethasone is safe, and when applied in a dose of 4mg/mL, it increases metabolic activity at the cellular level. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1809-48642018000200171 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1809-48642018000200171 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1055/s-0037-1604067 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Fundação Otorrinolaringologia |
publisher.none.fl_str_mv |
Fundação Otorrinolaringologia |
dc.source.none.fl_str_mv |
International Archives of Otorhinolaryngology v.22 n.2 2018 reponame:International Archives of Otorhinolaryngology instname:Fundação Otorrinolaringologia (FORL) instacron:FORL |
instname_str |
Fundação Otorrinolaringologia (FORL) |
instacron_str |
FORL |
institution |
FORL |
reponame_str |
International Archives of Otorhinolaryngology |
collection |
International Archives of Otorhinolaryngology |
repository.name.fl_str_mv |
International Archives of Otorhinolaryngology - Fundação Otorrinolaringologia (FORL) |
repository.mail.fl_str_mv |
||iaorl@iaorl.org||archives@internationalarchivesent.org||arquivos@forl.org.br |
_version_ |
1754203976086061056 |