Cytotoxicity analysis of electrostatically applied epoxy coating onto CO-CR alloy

Detalhes bibliográficos
Autor(a) principal: RAGONHA,Êrica Wehmuth
Data de Publicação: 2015
Outros Autores: MARTINEZ,Elizabeth Ferreira, MUZILLI,Carlos Alberto, MIRANDA,Milton Edson, OLIVIERI,Karina Andrea Novaes
Tipo de documento: Artigo
Idioma: eng
Título da fonte: RGO - Revista Gaúcha de Odontologia (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1981-86372015000300257
Resumo: Objective: To investigate the biological effect of a new method to camouflage the cobalt-chromium (CoCr) metal structure of an RPD, onto which an electrostatic paint was applied. Methods: In vitro cytotoxicity of epoxy Politherm NOBAC30C (Weg Industries SA, Santa Catarina, Brazil) in combination with polished CoCr was tested by placing it in contact with cultured human fibroblasts and comparing it with polystyrene (control surface). The cells were cultured in the presence of the test surfaces for 24, 48, 72, 94 and 120 hours. The number of viable and non-viable cells was established by manual counting. The Tukey test was used to statistically analyze cell counts between the groups. Results: The results showed that cell proliferation was similar between the groups (p =0.2174). It was observed that at 24, 48 and 72 h, there was no significant increase in cell proliferation in all groups. From 96 to 120 h, an increase in cell proliferation was observed in all groups, with no significant difference between them (p>0.05). Conclusion: The epoxy paint studied showed no cytotoxicity in vitro.
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spelling Cytotoxicity analysis of electrostatically applied epoxy coating onto CO-CR alloyDenture partial removableEpoxy compoundsEsthetics dentalToxicity tests Objective: To investigate the biological effect of a new method to camouflage the cobalt-chromium (CoCr) metal structure of an RPD, onto which an electrostatic paint was applied. Methods: In vitro cytotoxicity of epoxy Politherm NOBAC30C (Weg Industries SA, Santa Catarina, Brazil) in combination with polished CoCr was tested by placing it in contact with cultured human fibroblasts and comparing it with polystyrene (control surface). The cells were cultured in the presence of the test surfaces for 24, 48, 72, 94 and 120 hours. The number of viable and non-viable cells was established by manual counting. The Tukey test was used to statistically analyze cell counts between the groups. Results: The results showed that cell proliferation was similar between the groups (p =0.2174). It was observed that at 24, 48 and 72 h, there was no significant increase in cell proliferation in all groups. From 96 to 120 h, an increase in cell proliferation was observed in all groups, with no significant difference between them (p>0.05). Conclusion: The epoxy paint studied showed no cytotoxicity in vitro.Faculdade São Leopoldo Mandic2015-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1981-86372015000300257RGO - Revista Gaúcha de Odontologia v.63 n.3 2015reponame:RGO - Revista Gaúcha de Odontologia (Online)instname:Faculdade São Leopoldo Mandic (FSLM)instacron:FSLM10.1590/1981-863720150003000012838info:eu-repo/semantics/openAccessRAGONHA,Êrica WehmuthMARTINEZ,Elizabeth FerreiraMUZILLI,Carlos AlbertoMIRANDA,Milton EdsonOLIVIERI,Karina Andrea Novaeseng2019-08-06T00:00:00Zoai:scielo:S1981-86372015000300257Revistahttp://revodonto.bvsalud.org/scielo.php?script=sci_serial&pid=1981-8637&lng=pt&nrm=isohttps://old.scielo.br/oai/scielo-oai.php||contato@revistargo.com.br1981-86370103-6971opendoar:2019-08-06T00:00RGO - Revista Gaúcha de Odontologia (Online) - Faculdade São Leopoldo Mandic (FSLM)false
dc.title.none.fl_str_mv Cytotoxicity analysis of electrostatically applied epoxy coating onto CO-CR alloy
title Cytotoxicity analysis of electrostatically applied epoxy coating onto CO-CR alloy
spellingShingle Cytotoxicity analysis of electrostatically applied epoxy coating onto CO-CR alloy
RAGONHA,Êrica Wehmuth
Denture partial removable
Epoxy compounds
Esthetics dental
Toxicity tests
title_short Cytotoxicity analysis of electrostatically applied epoxy coating onto CO-CR alloy
title_full Cytotoxicity analysis of electrostatically applied epoxy coating onto CO-CR alloy
title_fullStr Cytotoxicity analysis of electrostatically applied epoxy coating onto CO-CR alloy
title_full_unstemmed Cytotoxicity analysis of electrostatically applied epoxy coating onto CO-CR alloy
title_sort Cytotoxicity analysis of electrostatically applied epoxy coating onto CO-CR alloy
author RAGONHA,Êrica Wehmuth
author_facet RAGONHA,Êrica Wehmuth
MARTINEZ,Elizabeth Ferreira
MUZILLI,Carlos Alberto
MIRANDA,Milton Edson
OLIVIERI,Karina Andrea Novaes
author_role author
author2 MARTINEZ,Elizabeth Ferreira
MUZILLI,Carlos Alberto
MIRANDA,Milton Edson
OLIVIERI,Karina Andrea Novaes
author2_role author
author
author
author
dc.contributor.author.fl_str_mv RAGONHA,Êrica Wehmuth
MARTINEZ,Elizabeth Ferreira
MUZILLI,Carlos Alberto
MIRANDA,Milton Edson
OLIVIERI,Karina Andrea Novaes
dc.subject.por.fl_str_mv Denture partial removable
Epoxy compounds
Esthetics dental
Toxicity tests
topic Denture partial removable
Epoxy compounds
Esthetics dental
Toxicity tests
description Objective: To investigate the biological effect of a new method to camouflage the cobalt-chromium (CoCr) metal structure of an RPD, onto which an electrostatic paint was applied. Methods: In vitro cytotoxicity of epoxy Politherm NOBAC30C (Weg Industries SA, Santa Catarina, Brazil) in combination with polished CoCr was tested by placing it in contact with cultured human fibroblasts and comparing it with polystyrene (control surface). The cells were cultured in the presence of the test surfaces for 24, 48, 72, 94 and 120 hours. The number of viable and non-viable cells was established by manual counting. The Tukey test was used to statistically analyze cell counts between the groups. Results: The results showed that cell proliferation was similar between the groups (p =0.2174). It was observed that at 24, 48 and 72 h, there was no significant increase in cell proliferation in all groups. From 96 to 120 h, an increase in cell proliferation was observed in all groups, with no significant difference between them (p>0.05). Conclusion: The epoxy paint studied showed no cytotoxicity in vitro.
publishDate 2015
dc.date.none.fl_str_mv 2015-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1981-86372015000300257
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1981-86372015000300257
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1981-863720150003000012838
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dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Faculdade São Leopoldo Mandic
publisher.none.fl_str_mv Faculdade São Leopoldo Mandic
dc.source.none.fl_str_mv RGO - Revista Gaúcha de Odontologia v.63 n.3 2015
reponame:RGO - Revista Gaúcha de Odontologia (Online)
instname:Faculdade São Leopoldo Mandic (FSLM)
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instname_str Faculdade São Leopoldo Mandic (FSLM)
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reponame_str RGO - Revista Gaúcha de Odontologia (Online)
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