Cytotoxicity analysis of electrostatically applied epoxy coating onto CO-CR alloy
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | RGO - Revista Gaúcha de Odontologia (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1981-86372015000300257 |
Resumo: | Objective: To investigate the biological effect of a new method to camouflage the cobalt-chromium (CoCr) metal structure of an RPD, onto which an electrostatic paint was applied. Methods: In vitro cytotoxicity of epoxy Politherm NOBAC30C (Weg Industries SA, Santa Catarina, Brazil) in combination with polished CoCr was tested by placing it in contact with cultured human fibroblasts and comparing it with polystyrene (control surface). The cells were cultured in the presence of the test surfaces for 24, 48, 72, 94 and 120 hours. The number of viable and non-viable cells was established by manual counting. The Tukey test was used to statistically analyze cell counts between the groups. Results: The results showed that cell proliferation was similar between the groups (p =0.2174). It was observed that at 24, 48 and 72 h, there was no significant increase in cell proliferation in all groups. From 96 to 120 h, an increase in cell proliferation was observed in all groups, with no significant difference between them (p>0.05). Conclusion: The epoxy paint studied showed no cytotoxicity in vitro. |
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Cytotoxicity analysis of electrostatically applied epoxy coating onto CO-CR alloyDenture partial removableEpoxy compoundsEsthetics dentalToxicity tests Objective: To investigate the biological effect of a new method to camouflage the cobalt-chromium (CoCr) metal structure of an RPD, onto which an electrostatic paint was applied. Methods: In vitro cytotoxicity of epoxy Politherm NOBAC30C (Weg Industries SA, Santa Catarina, Brazil) in combination with polished CoCr was tested by placing it in contact with cultured human fibroblasts and comparing it with polystyrene (control surface). The cells were cultured in the presence of the test surfaces for 24, 48, 72, 94 and 120 hours. The number of viable and non-viable cells was established by manual counting. The Tukey test was used to statistically analyze cell counts between the groups. Results: The results showed that cell proliferation was similar between the groups (p =0.2174). It was observed that at 24, 48 and 72 h, there was no significant increase in cell proliferation in all groups. From 96 to 120 h, an increase in cell proliferation was observed in all groups, with no significant difference between them (p>0.05). Conclusion: The epoxy paint studied showed no cytotoxicity in vitro.Faculdade São Leopoldo Mandic2015-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1981-86372015000300257RGO - Revista Gaúcha de Odontologia v.63 n.3 2015reponame:RGO - Revista Gaúcha de Odontologia (Online)instname:Faculdade São Leopoldo Mandic (FSLM)instacron:FSLM10.1590/1981-863720150003000012838info:eu-repo/semantics/openAccessRAGONHA,Êrica WehmuthMARTINEZ,Elizabeth FerreiraMUZILLI,Carlos AlbertoMIRANDA,Milton EdsonOLIVIERI,Karina Andrea Novaeseng2019-08-06T00:00:00Zoai:scielo:S1981-86372015000300257Revistahttp://revodonto.bvsalud.org/scielo.php?script=sci_serial&pid=1981-8637&lng=pt&nrm=isohttps://old.scielo.br/oai/scielo-oai.php||contato@revistargo.com.br1981-86370103-6971opendoar:2019-08-06T00:00RGO - Revista Gaúcha de Odontologia (Online) - Faculdade São Leopoldo Mandic (FSLM)false |
dc.title.none.fl_str_mv |
Cytotoxicity analysis of electrostatically applied epoxy coating onto CO-CR alloy |
title |
Cytotoxicity analysis of electrostatically applied epoxy coating onto CO-CR alloy |
spellingShingle |
Cytotoxicity analysis of electrostatically applied epoxy coating onto CO-CR alloy RAGONHA,Êrica Wehmuth Denture partial removable Epoxy compounds Esthetics dental Toxicity tests |
title_short |
Cytotoxicity analysis of electrostatically applied epoxy coating onto CO-CR alloy |
title_full |
Cytotoxicity analysis of electrostatically applied epoxy coating onto CO-CR alloy |
title_fullStr |
Cytotoxicity analysis of electrostatically applied epoxy coating onto CO-CR alloy |
title_full_unstemmed |
Cytotoxicity analysis of electrostatically applied epoxy coating onto CO-CR alloy |
title_sort |
Cytotoxicity analysis of electrostatically applied epoxy coating onto CO-CR alloy |
author |
RAGONHA,Êrica Wehmuth |
author_facet |
RAGONHA,Êrica Wehmuth MARTINEZ,Elizabeth Ferreira MUZILLI,Carlos Alberto MIRANDA,Milton Edson OLIVIERI,Karina Andrea Novaes |
author_role |
author |
author2 |
MARTINEZ,Elizabeth Ferreira MUZILLI,Carlos Alberto MIRANDA,Milton Edson OLIVIERI,Karina Andrea Novaes |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
RAGONHA,Êrica Wehmuth MARTINEZ,Elizabeth Ferreira MUZILLI,Carlos Alberto MIRANDA,Milton Edson OLIVIERI,Karina Andrea Novaes |
dc.subject.por.fl_str_mv |
Denture partial removable Epoxy compounds Esthetics dental Toxicity tests |
topic |
Denture partial removable Epoxy compounds Esthetics dental Toxicity tests |
description |
Objective: To investigate the biological effect of a new method to camouflage the cobalt-chromium (CoCr) metal structure of an RPD, onto which an electrostatic paint was applied. Methods: In vitro cytotoxicity of epoxy Politherm NOBAC30C (Weg Industries SA, Santa Catarina, Brazil) in combination with polished CoCr was tested by placing it in contact with cultured human fibroblasts and comparing it with polystyrene (control surface). The cells were cultured in the presence of the test surfaces for 24, 48, 72, 94 and 120 hours. The number of viable and non-viable cells was established by manual counting. The Tukey test was used to statistically analyze cell counts between the groups. Results: The results showed that cell proliferation was similar between the groups (p =0.2174). It was observed that at 24, 48 and 72 h, there was no significant increase in cell proliferation in all groups. From 96 to 120 h, an increase in cell proliferation was observed in all groups, with no significant difference between them (p>0.05). Conclusion: The epoxy paint studied showed no cytotoxicity in vitro. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1981-86372015000300257 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1981-86372015000300257 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1981-863720150003000012838 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Faculdade São Leopoldo Mandic |
publisher.none.fl_str_mv |
Faculdade São Leopoldo Mandic |
dc.source.none.fl_str_mv |
RGO - Revista Gaúcha de Odontologia v.63 n.3 2015 reponame:RGO - Revista Gaúcha de Odontologia (Online) instname:Faculdade São Leopoldo Mandic (FSLM) instacron:FSLM |
instname_str |
Faculdade São Leopoldo Mandic (FSLM) |
instacron_str |
FSLM |
institution |
FSLM |
reponame_str |
RGO - Revista Gaúcha de Odontologia (Online) |
collection |
RGO - Revista Gaúcha de Odontologia (Online) |
repository.name.fl_str_mv |
RGO - Revista Gaúcha de Odontologia (Online) - Faculdade São Leopoldo Mandic (FSLM) |
repository.mail.fl_str_mv |
||contato@revistargo.com.br |
_version_ |
1754204121224708096 |