Expression of the of STRO-1 and HSP-25 markers during odontogenesis
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | RGO - Revista Gaúcha de Odontologia (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1981-86372021000100322 |
Resumo: | ABSTRACT Objective: Therefore, the purpose of the currently study was to analyze the expression of the STRO-1 stem cell marker and the marker related to Hsp25 differentiation and dental development during odontogenesis in rats. Methods: Eighteen-day Wistar rat embryos and 7-day-old animals were analyzed morphologically via Hematoxylin and Eosin (HE) staining and via immunohistochemical marking for Stro-1 and Hsp25 in 5uM serial sections. Results: In the HE morphological analysis in 18-day embryos, the stage of tooth development was in the cap phase, while in the 7-day old animals it was in the bell phase. Conclusion: The expression of STRO-1 in the embryos was not found in the region of the dental papilla or enamel organ, however, in the 7-day-old animals, STRO-1-positive cells were found in the region of the dental follicle and dental papilla. As for the Hsp25, this exhibited weak marking in the cap phase while in the bell phase, there was a reaction, mainly in the odontoblasts. The expressions in the site of these markers vary according to the stage of tooth development. |
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RGO - Revista Gaúcha de Odontologia (Online) |
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Expression of the of STRO-1 and HSP-25 markers during odontogenesisImmunohistochemistryOdontogenesisStem cellsToothABSTRACT Objective: Therefore, the purpose of the currently study was to analyze the expression of the STRO-1 stem cell marker and the marker related to Hsp25 differentiation and dental development during odontogenesis in rats. Methods: Eighteen-day Wistar rat embryos and 7-day-old animals were analyzed morphologically via Hematoxylin and Eosin (HE) staining and via immunohistochemical marking for Stro-1 and Hsp25 in 5uM serial sections. Results: In the HE morphological analysis in 18-day embryos, the stage of tooth development was in the cap phase, while in the 7-day old animals it was in the bell phase. Conclusion: The expression of STRO-1 in the embryos was not found in the region of the dental papilla or enamel organ, however, in the 7-day-old animals, STRO-1-positive cells were found in the region of the dental follicle and dental papilla. As for the Hsp25, this exhibited weak marking in the cap phase while in the bell phase, there was a reaction, mainly in the odontoblasts. The expressions in the site of these markers vary according to the stage of tooth development.Faculdade São Leopoldo Mandic2021-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1981-86372021000100322RGO - Revista Gaúcha de Odontologia v.69 2021reponame:RGO - Revista Gaúcha de Odontologia (Online)instname:Faculdade São Leopoldo Mandic (FSLM)instacron:FSLM10.1590/1981-863720210005420200017info:eu-repo/semantics/openAccessREZENDE,Karla MayraZUIM,Juliana PereiraBÖNECKER,Marceloeng2021-11-05T00:00:00Zoai:scielo:S1981-86372021000100322Revistahttp://revodonto.bvsalud.org/scielo.php?script=sci_serial&pid=1981-8637&lng=pt&nrm=isohttps://old.scielo.br/oai/scielo-oai.php||contato@revistargo.com.br1981-86370103-6971opendoar:2021-11-05T00:00RGO - Revista Gaúcha de Odontologia (Online) - Faculdade São Leopoldo Mandic (FSLM)false |
dc.title.none.fl_str_mv |
Expression of the of STRO-1 and HSP-25 markers during odontogenesis |
title |
Expression of the of STRO-1 and HSP-25 markers during odontogenesis |
spellingShingle |
Expression of the of STRO-1 and HSP-25 markers during odontogenesis REZENDE,Karla Mayra Immunohistochemistry Odontogenesis Stem cells Tooth |
title_short |
Expression of the of STRO-1 and HSP-25 markers during odontogenesis |
title_full |
Expression of the of STRO-1 and HSP-25 markers during odontogenesis |
title_fullStr |
Expression of the of STRO-1 and HSP-25 markers during odontogenesis |
title_full_unstemmed |
Expression of the of STRO-1 and HSP-25 markers during odontogenesis |
title_sort |
Expression of the of STRO-1 and HSP-25 markers during odontogenesis |
author |
REZENDE,Karla Mayra |
author_facet |
REZENDE,Karla Mayra ZUIM,Juliana Pereira BÖNECKER,Marcelo |
author_role |
author |
author2 |
ZUIM,Juliana Pereira BÖNECKER,Marcelo |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
REZENDE,Karla Mayra ZUIM,Juliana Pereira BÖNECKER,Marcelo |
dc.subject.por.fl_str_mv |
Immunohistochemistry Odontogenesis Stem cells Tooth |
topic |
Immunohistochemistry Odontogenesis Stem cells Tooth |
description |
ABSTRACT Objective: Therefore, the purpose of the currently study was to analyze the expression of the STRO-1 stem cell marker and the marker related to Hsp25 differentiation and dental development during odontogenesis in rats. Methods: Eighteen-day Wistar rat embryos and 7-day-old animals were analyzed morphologically via Hematoxylin and Eosin (HE) staining and via immunohistochemical marking for Stro-1 and Hsp25 in 5uM serial sections. Results: In the HE morphological analysis in 18-day embryos, the stage of tooth development was in the cap phase, while in the 7-day old animals it was in the bell phase. Conclusion: The expression of STRO-1 in the embryos was not found in the region of the dental papilla or enamel organ, however, in the 7-day-old animals, STRO-1-positive cells were found in the region of the dental follicle and dental papilla. As for the Hsp25, this exhibited weak marking in the cap phase while in the bell phase, there was a reaction, mainly in the odontoblasts. The expressions in the site of these markers vary according to the stage of tooth development. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1981-86372021000100322 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1981-86372021000100322 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1981-863720210005420200017 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Faculdade São Leopoldo Mandic |
publisher.none.fl_str_mv |
Faculdade São Leopoldo Mandic |
dc.source.none.fl_str_mv |
RGO - Revista Gaúcha de Odontologia v.69 2021 reponame:RGO - Revista Gaúcha de Odontologia (Online) instname:Faculdade São Leopoldo Mandic (FSLM) instacron:FSLM |
instname_str |
Faculdade São Leopoldo Mandic (FSLM) |
instacron_str |
FSLM |
institution |
FSLM |
reponame_str |
RGO - Revista Gaúcha de Odontologia (Online) |
collection |
RGO - Revista Gaúcha de Odontologia (Online) |
repository.name.fl_str_mv |
RGO - Revista Gaúcha de Odontologia (Online) - Faculdade São Leopoldo Mandic (FSLM) |
repository.mail.fl_str_mv |
||contato@revistargo.com.br |
_version_ |
1754204122815397888 |