Matricaria Recutita Extract (Chamomile) to reduce Candida Albicans and Entrobacter Cloacae biofilms: in vitro study
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | RGO - Revista Gaúcha de Odontologia (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1981-86372018000200122 |
Resumo: | ABSTRACT Objective: This research study aimed at evaluating the inhibitory activity of Matricaria recutira (chamomile) hydroalcoholic extract on Candida albicans and Enterobacter cloacae biofilms. Methods: C. albicans and E. cloacae biofilms with thirty-hour formation were submitted, for five minutes, to 100, 200 and 300 mg / mL of M. recutita hydroalcoholic extract, chlorhexidine digluconate 0.12% (Periogard® - inhibition control) or sterile distilled water (growth control). Subsequently, they were washed and divided into two groups to determine the microbial viability: G/UFC - counting of colony forming units (cfu) in agar and G/DNA - quantification of viable DNA with violet crystal dye by spectrophotometry. Results: M. recutita extract at 300 mg/mL reduced significantly (p <0.01) the E. cloacae cfu/mL number in biofilm with results similar to chlorhexidine 0.12%, while extracts at 100 and 200 mg/mL did not have the same effectiveness. The amount of E. cloacae viable DNA was reduced (p <0.05) in all the M. recutita extract concentrations and chlorhexidine. There was no significant difference (p = 0.565) in the cfu/mL number or in the amount of viable DNA (p = 0.8094) in C. albicans biofilm when compared to untreated biofilm (control) or, even, between the extracts when compared to each other or to chlorhexidine 0.12%. Conclusion: 300 mg/mL M. recutita extract reduced significantly the E. cloacae biofilm but not the C. albicans, both with a similar result to chlorhexidine 0.12% (Periogar®). |
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Matricaria Recutita Extract (Chamomile) to reduce Candida Albicans and Entrobacter Cloacae biofilms: in vitro studyBiofilmsCandida AlbicansEnterobacter CloacaeMatricariaPhytotherapyABSTRACT Objective: This research study aimed at evaluating the inhibitory activity of Matricaria recutira (chamomile) hydroalcoholic extract on Candida albicans and Enterobacter cloacae biofilms. Methods: C. albicans and E. cloacae biofilms with thirty-hour formation were submitted, for five minutes, to 100, 200 and 300 mg / mL of M. recutita hydroalcoholic extract, chlorhexidine digluconate 0.12% (Periogard® - inhibition control) or sterile distilled water (growth control). Subsequently, they were washed and divided into two groups to determine the microbial viability: G/UFC - counting of colony forming units (cfu) in agar and G/DNA - quantification of viable DNA with violet crystal dye by spectrophotometry. Results: M. recutita extract at 300 mg/mL reduced significantly (p <0.01) the E. cloacae cfu/mL number in biofilm with results similar to chlorhexidine 0.12%, while extracts at 100 and 200 mg/mL did not have the same effectiveness. The amount of E. cloacae viable DNA was reduced (p <0.05) in all the M. recutita extract concentrations and chlorhexidine. There was no significant difference (p = 0.565) in the cfu/mL number or in the amount of viable DNA (p = 0.8094) in C. albicans biofilm when compared to untreated biofilm (control) or, even, between the extracts when compared to each other or to chlorhexidine 0.12%. Conclusion: 300 mg/mL M. recutita extract reduced significantly the E. cloacae biofilm but not the C. albicans, both with a similar result to chlorhexidine 0.12% (Periogar®).Faculdade São Leopoldo Mandic2018-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1981-86372018000200122RGO - Revista Gaúcha de Odontologia v.66 n.2 2018reponame:RGO - Revista Gaúcha de Odontologia (Online)instname:Faculdade São Leopoldo Mandic (FSLM)instacron:FSLM10.1590/1981-863720180002000033328info:eu-repo/semantics/openAccessPEQUENO,Maitê AlvesSILVESTRE,Marissa RodriguesAMÊNDOLA,IsabelaSILVA,Célia Regina Gonçalves eLEÃO,Mariella Vieira PereiraSANTOS,Silvana Soleo Ferreira doseng2019-08-06T00:00:00Zoai:scielo:S1981-86372018000200122Revistahttp://revodonto.bvsalud.org/scielo.php?script=sci_serial&pid=1981-8637&lng=pt&nrm=isohttps://old.scielo.br/oai/scielo-oai.php||contato@revistargo.com.br1981-86370103-6971opendoar:2019-08-06T00:00RGO - Revista Gaúcha de Odontologia (Online) - Faculdade São Leopoldo Mandic (FSLM)false |
dc.title.none.fl_str_mv |
Matricaria Recutita Extract (Chamomile) to reduce Candida Albicans and Entrobacter Cloacae biofilms: in vitro study |
title |
Matricaria Recutita Extract (Chamomile) to reduce Candida Albicans and Entrobacter Cloacae biofilms: in vitro study |
spellingShingle |
Matricaria Recutita Extract (Chamomile) to reduce Candida Albicans and Entrobacter Cloacae biofilms: in vitro study PEQUENO,Maitê Alves Biofilms Candida Albicans Enterobacter Cloacae Matricaria Phytotherapy |
title_short |
Matricaria Recutita Extract (Chamomile) to reduce Candida Albicans and Entrobacter Cloacae biofilms: in vitro study |
title_full |
Matricaria Recutita Extract (Chamomile) to reduce Candida Albicans and Entrobacter Cloacae biofilms: in vitro study |
title_fullStr |
Matricaria Recutita Extract (Chamomile) to reduce Candida Albicans and Entrobacter Cloacae biofilms: in vitro study |
title_full_unstemmed |
Matricaria Recutita Extract (Chamomile) to reduce Candida Albicans and Entrobacter Cloacae biofilms: in vitro study |
title_sort |
Matricaria Recutita Extract (Chamomile) to reduce Candida Albicans and Entrobacter Cloacae biofilms: in vitro study |
author |
PEQUENO,Maitê Alves |
author_facet |
PEQUENO,Maitê Alves SILVESTRE,Marissa Rodrigues AMÊNDOLA,Isabela SILVA,Célia Regina Gonçalves e LEÃO,Mariella Vieira Pereira SANTOS,Silvana Soleo Ferreira dos |
author_role |
author |
author2 |
SILVESTRE,Marissa Rodrigues AMÊNDOLA,Isabela SILVA,Célia Regina Gonçalves e LEÃO,Mariella Vieira Pereira SANTOS,Silvana Soleo Ferreira dos |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
PEQUENO,Maitê Alves SILVESTRE,Marissa Rodrigues AMÊNDOLA,Isabela SILVA,Célia Regina Gonçalves e LEÃO,Mariella Vieira Pereira SANTOS,Silvana Soleo Ferreira dos |
dc.subject.por.fl_str_mv |
Biofilms Candida Albicans Enterobacter Cloacae Matricaria Phytotherapy |
topic |
Biofilms Candida Albicans Enterobacter Cloacae Matricaria Phytotherapy |
description |
ABSTRACT Objective: This research study aimed at evaluating the inhibitory activity of Matricaria recutira (chamomile) hydroalcoholic extract on Candida albicans and Enterobacter cloacae biofilms. Methods: C. albicans and E. cloacae biofilms with thirty-hour formation were submitted, for five minutes, to 100, 200 and 300 mg / mL of M. recutita hydroalcoholic extract, chlorhexidine digluconate 0.12% (Periogard® - inhibition control) or sterile distilled water (growth control). Subsequently, they were washed and divided into two groups to determine the microbial viability: G/UFC - counting of colony forming units (cfu) in agar and G/DNA - quantification of viable DNA with violet crystal dye by spectrophotometry. Results: M. recutita extract at 300 mg/mL reduced significantly (p <0.01) the E. cloacae cfu/mL number in biofilm with results similar to chlorhexidine 0.12%, while extracts at 100 and 200 mg/mL did not have the same effectiveness. The amount of E. cloacae viable DNA was reduced (p <0.05) in all the M. recutita extract concentrations and chlorhexidine. There was no significant difference (p = 0.565) in the cfu/mL number or in the amount of viable DNA (p = 0.8094) in C. albicans biofilm when compared to untreated biofilm (control) or, even, between the extracts when compared to each other or to chlorhexidine 0.12%. Conclusion: 300 mg/mL M. recutita extract reduced significantly the E. cloacae biofilm but not the C. albicans, both with a similar result to chlorhexidine 0.12% (Periogar®). |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1981-86372018000200122 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1981-86372018000200122 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1981-863720180002000033328 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Faculdade São Leopoldo Mandic |
publisher.none.fl_str_mv |
Faculdade São Leopoldo Mandic |
dc.source.none.fl_str_mv |
RGO - Revista Gaúcha de Odontologia v.66 n.2 2018 reponame:RGO - Revista Gaúcha de Odontologia (Online) instname:Faculdade São Leopoldo Mandic (FSLM) instacron:FSLM |
instname_str |
Faculdade São Leopoldo Mandic (FSLM) |
instacron_str |
FSLM |
institution |
FSLM |
reponame_str |
RGO - Revista Gaúcha de Odontologia (Online) |
collection |
RGO - Revista Gaúcha de Odontologia (Online) |
repository.name.fl_str_mv |
RGO - Revista Gaúcha de Odontologia (Online) - Faculdade São Leopoldo Mandic (FSLM) |
repository.mail.fl_str_mv |
||contato@revistargo.com.br |
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1754204122004848640 |