Association between Estrogen, Vitamin D and Microrna17 Gene Polymorphisms and Periapical Lesions

Detalhes bibliográficos
Autor(a) principal: Silva-Sousa,Alice Corrêa
Data de Publicação: 2020
Outros Autores: Mazzi-Chaves,Jardel Francisco, Freitas,Jessica Vavassori, Salles,Alessandro Guimarães, Segato,Raquel Assed Bezerra da Silva, Silva,Léa Assed Bezerra da, Antunes,Lívia Azeredo Alves, Antunes,Leonardo Santos, Baratto-Filho,Flares, Sousa-Neto,Manoel Damião, Küchler,Erika Calvano
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Dental Journal
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402020000100019
Resumo: Abstract This study evaluated the association between polymorphisms in genes encoding estrogen receptors 1 (ESR1) and 2 (ESR2), vitamin D receptor (VDR) and in microRNA17 (which binds to ESR1 and VDR) with persistent apical periodontitis (PAP) after the endodontic treatment. We included 162 patients who completed endodontic treatment at least one year ago and presented apical periodontitis at the beginning of the root canal therapy. Clinical and radiographic exams were performed to evaluate the presence of PAP or healthy periradicular tissues (healed). Saliva samples were collected as a genomic DNA. The genotyping of ESR1 (rs2234693 and rs9340799), ESR2 (rs1256049 and rs4986938), VDR (rs739837 and rs2228570) and miRNA17 (rs4284505) were performed by real-time PCR. Chi-square test was used to the distribution of genotype and allele frequencies. Haplotype analysis was also performed. Eighty-nine patients were included in the “healed” group and 73 in the “PAP” group. No association was found between the allelic and genotypic polymorphisms studied and PAP (p>0.05). Haplotype analysis also did not demonstrated an association (p>0.05). In conclusion, the genetic polymorphisms in ESR1, ESR2, VDR and miRNA17 are not associated with PAP.
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spelling Association between Estrogen, Vitamin D and Microrna17 Gene Polymorphisms and Periapical Lesionspersistent apical periodontitisgenetic polymorphismsvitamin destrogenAbstract This study evaluated the association between polymorphisms in genes encoding estrogen receptors 1 (ESR1) and 2 (ESR2), vitamin D receptor (VDR) and in microRNA17 (which binds to ESR1 and VDR) with persistent apical periodontitis (PAP) after the endodontic treatment. We included 162 patients who completed endodontic treatment at least one year ago and presented apical periodontitis at the beginning of the root canal therapy. Clinical and radiographic exams were performed to evaluate the presence of PAP or healthy periradicular tissues (healed). Saliva samples were collected as a genomic DNA. The genotyping of ESR1 (rs2234693 and rs9340799), ESR2 (rs1256049 and rs4986938), VDR (rs739837 and rs2228570) and miRNA17 (rs4284505) were performed by real-time PCR. Chi-square test was used to the distribution of genotype and allele frequencies. Haplotype analysis was also performed. Eighty-nine patients were included in the “healed” group and 73 in the “PAP” group. No association was found between the allelic and genotypic polymorphisms studied and PAP (p>0.05). Haplotype analysis also did not demonstrated an association (p>0.05). In conclusion, the genetic polymorphisms in ESR1, ESR2, VDR and miRNA17 are not associated with PAP.Fundação Odontológica de Ribeirão Preto2020-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402020000100019Brazilian Dental Journal v.31 n.1 2020reponame:Brazilian Dental Journalinstname:Fundação Odontológica de Ribeirão Preto (FUNORP)instacron:FUNORP10.1590/0103-644020200info:eu-repo/semantics/openAccessSilva-Sousa,Alice CorrêaMazzi-Chaves,Jardel FranciscoFreitas,Jessica VavassoriSalles,Alessandro GuimarãesSegato,Raquel Assed Bezerra da SilvaSilva,Léa Assed Bezerra daAntunes,Lívia Azeredo AlvesAntunes,Leonardo SantosBaratto-Filho,FlaresSousa-Neto,Manoel DamiãoKüchler,Erika Calvanoeng2020-03-02T00:00:00Zoai:scielo:S0103-64402020000100019Revistahttps://www.scielo.br/j/bdj/https://old.scielo.br/oai/scielo-oai.phpbdj@forp.usp.br||sergio@fosjc.unesp.br1806-47600103-6440opendoar:2020-03-02T00:00Brazilian Dental Journal - Fundação Odontológica de Ribeirão Preto (FUNORP)false
dc.title.none.fl_str_mv Association between Estrogen, Vitamin D and Microrna17 Gene Polymorphisms and Periapical Lesions
title Association between Estrogen, Vitamin D and Microrna17 Gene Polymorphisms and Periapical Lesions
spellingShingle Association between Estrogen, Vitamin D and Microrna17 Gene Polymorphisms and Periapical Lesions
Silva-Sousa,Alice Corrêa
persistent apical periodontitis
genetic polymorphisms
vitamin d
estrogen
title_short Association between Estrogen, Vitamin D and Microrna17 Gene Polymorphisms and Periapical Lesions
title_full Association between Estrogen, Vitamin D and Microrna17 Gene Polymorphisms and Periapical Lesions
title_fullStr Association between Estrogen, Vitamin D and Microrna17 Gene Polymorphisms and Periapical Lesions
title_full_unstemmed Association between Estrogen, Vitamin D and Microrna17 Gene Polymorphisms and Periapical Lesions
title_sort Association between Estrogen, Vitamin D and Microrna17 Gene Polymorphisms and Periapical Lesions
author Silva-Sousa,Alice Corrêa
author_facet Silva-Sousa,Alice Corrêa
Mazzi-Chaves,Jardel Francisco
Freitas,Jessica Vavassori
Salles,Alessandro Guimarães
Segato,Raquel Assed Bezerra da Silva
Silva,Léa Assed Bezerra da
Antunes,Lívia Azeredo Alves
Antunes,Leonardo Santos
Baratto-Filho,Flares
Sousa-Neto,Manoel Damião
Küchler,Erika Calvano
author_role author
author2 Mazzi-Chaves,Jardel Francisco
Freitas,Jessica Vavassori
Salles,Alessandro Guimarães
Segato,Raquel Assed Bezerra da Silva
Silva,Léa Assed Bezerra da
Antunes,Lívia Azeredo Alves
Antunes,Leonardo Santos
Baratto-Filho,Flares
Sousa-Neto,Manoel Damião
Küchler,Erika Calvano
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Silva-Sousa,Alice Corrêa
Mazzi-Chaves,Jardel Francisco
Freitas,Jessica Vavassori
Salles,Alessandro Guimarães
Segato,Raquel Assed Bezerra da Silva
Silva,Léa Assed Bezerra da
Antunes,Lívia Azeredo Alves
Antunes,Leonardo Santos
Baratto-Filho,Flares
Sousa-Neto,Manoel Damião
Küchler,Erika Calvano
dc.subject.por.fl_str_mv persistent apical periodontitis
genetic polymorphisms
vitamin d
estrogen
topic persistent apical periodontitis
genetic polymorphisms
vitamin d
estrogen
description Abstract This study evaluated the association between polymorphisms in genes encoding estrogen receptors 1 (ESR1) and 2 (ESR2), vitamin D receptor (VDR) and in microRNA17 (which binds to ESR1 and VDR) with persistent apical periodontitis (PAP) after the endodontic treatment. We included 162 patients who completed endodontic treatment at least one year ago and presented apical periodontitis at the beginning of the root canal therapy. Clinical and radiographic exams were performed to evaluate the presence of PAP or healthy periradicular tissues (healed). Saliva samples were collected as a genomic DNA. The genotyping of ESR1 (rs2234693 and rs9340799), ESR2 (rs1256049 and rs4986938), VDR (rs739837 and rs2228570) and miRNA17 (rs4284505) were performed by real-time PCR. Chi-square test was used to the distribution of genotype and allele frequencies. Haplotype analysis was also performed. Eighty-nine patients were included in the “healed” group and 73 in the “PAP” group. No association was found between the allelic and genotypic polymorphisms studied and PAP (p>0.05). Haplotype analysis also did not demonstrated an association (p>0.05). In conclusion, the genetic polymorphisms in ESR1, ESR2, VDR and miRNA17 are not associated with PAP.
publishDate 2020
dc.date.none.fl_str_mv 2020-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402020000100019
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402020000100019
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0103-644020200
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Fundação Odontológica de Ribeirão Preto
publisher.none.fl_str_mv Fundação Odontológica de Ribeirão Preto
dc.source.none.fl_str_mv Brazilian Dental Journal v.31 n.1 2020
reponame:Brazilian Dental Journal
instname:Fundação Odontológica de Ribeirão Preto (FUNORP)
instacron:FUNORP
instname_str Fundação Odontológica de Ribeirão Preto (FUNORP)
instacron_str FUNORP
institution FUNORP
reponame_str Brazilian Dental Journal
collection Brazilian Dental Journal
repository.name.fl_str_mv Brazilian Dental Journal - Fundação Odontológica de Ribeirão Preto (FUNORP)
repository.mail.fl_str_mv bdj@forp.usp.br||sergio@fosjc.unesp.br
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