Long-Term Cytotoxicity, pH and Dissolution Rate of AH Plus and MTA Fillapex
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Dental Journal |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402016000400419 |
Resumo: | Abstract The aim of the present study was to verify the long-term cytotoxic effects of the MTA Fillapex and to compare them with AH Plus. Dissolution rate and pH were also evaluated. Human osteoblast cells were incubated with elutes of fresh specimens from AH Plus and MTA Fillapex, and with elutes of the same specimens for 4 successive weeks. Elute's pH was evaluated at each time point. A multiparametric cell viability assay was performed. For dissolution rate, ISO methodology was used. The results were analyzed by one-way analysis of variance, complemented with the Tukey post-test (p<0.05). No significant difference was found among the materials when fresh mixed (p>0.05). After 1 week, AH Plus became non-cytotoxic on all three evaluated parameters. Conversely, MTA Fillapex remained severely and mildly cytotoxic over the entire experimental period (p<0.05). The dissolution rate of AH Plus was significantly lower than MTA Fillapex at all time points (p>0.05). The pH of AH Plus was significantly lower than MTA Fillapex at the second and third week (p<0.05). In the other tested time points no statistical difference was observed. In conclusion, MTA Fillapex remained cytotoxic after 4 weeks and its cytotoxicity may be related to the high dissolution rate of this material. |
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Brazilian Dental Journal |
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Long-Term Cytotoxicity, pH and Dissolution Rate of AH Plus and MTA FillapexAH Pluscytotoxicitycell cultureMTA FillapexAbstract The aim of the present study was to verify the long-term cytotoxic effects of the MTA Fillapex and to compare them with AH Plus. Dissolution rate and pH were also evaluated. Human osteoblast cells were incubated with elutes of fresh specimens from AH Plus and MTA Fillapex, and with elutes of the same specimens for 4 successive weeks. Elute's pH was evaluated at each time point. A multiparametric cell viability assay was performed. For dissolution rate, ISO methodology was used. The results were analyzed by one-way analysis of variance, complemented with the Tukey post-test (p<0.05). No significant difference was found among the materials when fresh mixed (p>0.05). After 1 week, AH Plus became non-cytotoxic on all three evaluated parameters. Conversely, MTA Fillapex remained severely and mildly cytotoxic over the entire experimental period (p<0.05). The dissolution rate of AH Plus was significantly lower than MTA Fillapex at all time points (p>0.05). The pH of AH Plus was significantly lower than MTA Fillapex at the second and third week (p<0.05). In the other tested time points no statistical difference was observed. In conclusion, MTA Fillapex remained cytotoxic after 4 weeks and its cytotoxicity may be related to the high dissolution rate of this material.Fundação Odontológica de Ribeirão Preto2016-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402016000400419Brazilian Dental Journal v.27 n.4 2016reponame:Brazilian Dental Journalinstname:Fundação Odontológica de Ribeirão Preto (FUNORP)instacron:FUNORP10.1590/0103-6440201600735info:eu-repo/semantics/openAccessSilva,Emmanuel João Nogueira Leal daAccorsi-Mendonça,ThaisPedrosa,Ana CarolinaGranjeiro,José MauroZaia,Alexandre A.eng2016-09-20T00:00:00Zoai:scielo:S0103-64402016000400419Revistahttps://www.scielo.br/j/bdj/https://old.scielo.br/oai/scielo-oai.phpbdj@forp.usp.br||sergio@fosjc.unesp.br1806-47600103-6440opendoar:2016-09-20T00:00Brazilian Dental Journal - Fundação Odontológica de Ribeirão Preto (FUNORP)false |
dc.title.none.fl_str_mv |
Long-Term Cytotoxicity, pH and Dissolution Rate of AH Plus and MTA Fillapex |
title |
Long-Term Cytotoxicity, pH and Dissolution Rate of AH Plus and MTA Fillapex |
spellingShingle |
Long-Term Cytotoxicity, pH and Dissolution Rate of AH Plus and MTA Fillapex Silva,Emmanuel João Nogueira Leal da AH Plus cytotoxicity cell culture MTA Fillapex |
title_short |
Long-Term Cytotoxicity, pH and Dissolution Rate of AH Plus and MTA Fillapex |
title_full |
Long-Term Cytotoxicity, pH and Dissolution Rate of AH Plus and MTA Fillapex |
title_fullStr |
Long-Term Cytotoxicity, pH and Dissolution Rate of AH Plus and MTA Fillapex |
title_full_unstemmed |
Long-Term Cytotoxicity, pH and Dissolution Rate of AH Plus and MTA Fillapex |
title_sort |
Long-Term Cytotoxicity, pH and Dissolution Rate of AH Plus and MTA Fillapex |
author |
Silva,Emmanuel João Nogueira Leal da |
author_facet |
Silva,Emmanuel João Nogueira Leal da Accorsi-Mendonça,Thais Pedrosa,Ana Carolina Granjeiro,José Mauro Zaia,Alexandre A. |
author_role |
author |
author2 |
Accorsi-Mendonça,Thais Pedrosa,Ana Carolina Granjeiro,José Mauro Zaia,Alexandre A. |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Silva,Emmanuel João Nogueira Leal da Accorsi-Mendonça,Thais Pedrosa,Ana Carolina Granjeiro,José Mauro Zaia,Alexandre A. |
dc.subject.por.fl_str_mv |
AH Plus cytotoxicity cell culture MTA Fillapex |
topic |
AH Plus cytotoxicity cell culture MTA Fillapex |
description |
Abstract The aim of the present study was to verify the long-term cytotoxic effects of the MTA Fillapex and to compare them with AH Plus. Dissolution rate and pH were also evaluated. Human osteoblast cells were incubated with elutes of fresh specimens from AH Plus and MTA Fillapex, and with elutes of the same specimens for 4 successive weeks. Elute's pH was evaluated at each time point. A multiparametric cell viability assay was performed. For dissolution rate, ISO methodology was used. The results were analyzed by one-way analysis of variance, complemented with the Tukey post-test (p<0.05). No significant difference was found among the materials when fresh mixed (p>0.05). After 1 week, AH Plus became non-cytotoxic on all three evaluated parameters. Conversely, MTA Fillapex remained severely and mildly cytotoxic over the entire experimental period (p<0.05). The dissolution rate of AH Plus was significantly lower than MTA Fillapex at all time points (p>0.05). The pH of AH Plus was significantly lower than MTA Fillapex at the second and third week (p<0.05). In the other tested time points no statistical difference was observed. In conclusion, MTA Fillapex remained cytotoxic after 4 weeks and its cytotoxicity may be related to the high dissolution rate of this material. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-08-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402016000400419 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402016000400419 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0103-6440201600735 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Fundação Odontológica de Ribeirão Preto |
publisher.none.fl_str_mv |
Fundação Odontológica de Ribeirão Preto |
dc.source.none.fl_str_mv |
Brazilian Dental Journal v.27 n.4 2016 reponame:Brazilian Dental Journal instname:Fundação Odontológica de Ribeirão Preto (FUNORP) instacron:FUNORP |
instname_str |
Fundação Odontológica de Ribeirão Preto (FUNORP) |
instacron_str |
FUNORP |
institution |
FUNORP |
reponame_str |
Brazilian Dental Journal |
collection |
Brazilian Dental Journal |
repository.name.fl_str_mv |
Brazilian Dental Journal - Fundação Odontológica de Ribeirão Preto (FUNORP) |
repository.mail.fl_str_mv |
bdj@forp.usp.br||sergio@fosjc.unesp.br |
_version_ |
1754204094331879424 |