Effect of PDGF-BB, IGF-I Growth Factors and their Combination Carried by Liposomes in Tooth Socket Healing
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Dental Journal |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402013000400299 |
Resumo: | This work evaluated the bone-forming potential of the platelet-derived growth factor isoform BB (PDGF-BB), insulin-like growth factor I (IGF-I), and mixed PDGF-BB/IGF-I delivered in liposomes compared with phosphate buffered saline (PBS), in the healing process of rat tooth sockets. One hundred and twelve Wistar rats were randomized into 7 groups of 16 animals each and were evaluated at 3, 7, 14 and 21 days after extraction of the maxillary second molars. The left sockets were treated with PBS (P), empty liposome (L), IGF-I in PBS (IP), IGF-I in liposome (IL), PDGF-BB in PBS (PDP), PDGF-BB in liposome (PDL) and both growth factors (GFs) together within liposomes (PDIL). The right sockets were filled with blood clot (BC). Histological and histomorphometric analyses were used to evaluate the formation of new bone and blood vessels. Immunohistochemistry was performed to evaluate the expression of osteocalcin and vascular endothelial growth factor (VEGF) during bone repair. Data were tested statistically using a Tukey's test according to a Dunn's analysis and Mann-Whitney U test followed by Kruskal-Wallis one-way analysis. Results were considered significant when p<0.05. A significantly higher percentage of bone trabeculae and a higher number of blood vessels were observed in the IL, PDL and PDIL groups (p<0.05). However, these GF-liposome groups had statistically similar results. Immunohistochemical assays first detected osteocalcin and VEGF expression at 3 days followed by a peak at 7 days. Lower immunoreactivity levels were observed in the BC, L, P, IP and PDP groups compared with the IL, PDL and PDIL groups (p<0.05). The results suggest that GFs carried by liposomes, either in isolated or mixed forms, enhanced the healing process in rat tooth sockets. The differential expression of the osteogenic markers VEGF and osteocalcin in the early phases of bone healing support these findings. |
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Brazilian Dental Journal |
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Effect of PDGF-BB, IGF-I Growth Factors and their Combination Carried by Liposomes in Tooth Socket Healinggrowth factorPDGFIGFliposomebone repairThis work evaluated the bone-forming potential of the platelet-derived growth factor isoform BB (PDGF-BB), insulin-like growth factor I (IGF-I), and mixed PDGF-BB/IGF-I delivered in liposomes compared with phosphate buffered saline (PBS), in the healing process of rat tooth sockets. One hundred and twelve Wistar rats were randomized into 7 groups of 16 animals each and were evaluated at 3, 7, 14 and 21 days after extraction of the maxillary second molars. The left sockets were treated with PBS (P), empty liposome (L), IGF-I in PBS (IP), IGF-I in liposome (IL), PDGF-BB in PBS (PDP), PDGF-BB in liposome (PDL) and both growth factors (GFs) together within liposomes (PDIL). The right sockets were filled with blood clot (BC). Histological and histomorphometric analyses were used to evaluate the formation of new bone and blood vessels. Immunohistochemistry was performed to evaluate the expression of osteocalcin and vascular endothelial growth factor (VEGF) during bone repair. Data were tested statistically using a Tukey's test according to a Dunn's analysis and Mann-Whitney U test followed by Kruskal-Wallis one-way analysis. Results were considered significant when p<0.05. A significantly higher percentage of bone trabeculae and a higher number of blood vessels were observed in the IL, PDL and PDIL groups (p<0.05). However, these GF-liposome groups had statistically similar results. Immunohistochemical assays first detected osteocalcin and VEGF expression at 3 days followed by a peak at 7 days. Lower immunoreactivity levels were observed in the BC, L, P, IP and PDP groups compared with the IL, PDL and PDIL groups (p<0.05). The results suggest that GFs carried by liposomes, either in isolated or mixed forms, enhanced the healing process in rat tooth sockets. The differential expression of the osteogenic markers VEGF and osteocalcin in the early phases of bone healing support these findings.Fundação Odontológica de Ribeirão Preto2013-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402013000400299Brazilian Dental Journal v.24 n.4 2013reponame:Brazilian Dental Journalinstname:Fundação Odontológica de Ribeirão Preto (FUNORP)instacron:FUNORP10.1590/0103-6440201302238info:eu-repo/semantics/openAccessAbreu,Fernando Antonio Mauad deFerreira,Cynthia LopesSilva,Gerluza Aparecida BorgesPaulo,Camila de OliveiraMiziara,Melissa NunesSilveira,Frank FerreiraAlves,Jose Bentoeng2013-10-16T00:00:00Zoai:scielo:S0103-64402013000400299Revistahttps://www.scielo.br/j/bdj/https://old.scielo.br/oai/scielo-oai.phpbdj@forp.usp.br||sergio@fosjc.unesp.br1806-47600103-6440opendoar:2013-10-16T00:00Brazilian Dental Journal - Fundação Odontológica de Ribeirão Preto (FUNORP)false |
dc.title.none.fl_str_mv |
Effect of PDGF-BB, IGF-I Growth Factors and their Combination Carried by Liposomes in Tooth Socket Healing |
title |
Effect of PDGF-BB, IGF-I Growth Factors and their Combination Carried by Liposomes in Tooth Socket Healing |
spellingShingle |
Effect of PDGF-BB, IGF-I Growth Factors and their Combination Carried by Liposomes in Tooth Socket Healing Abreu,Fernando Antonio Mauad de growth factor PDGF IGF liposome bone repair |
title_short |
Effect of PDGF-BB, IGF-I Growth Factors and their Combination Carried by Liposomes in Tooth Socket Healing |
title_full |
Effect of PDGF-BB, IGF-I Growth Factors and their Combination Carried by Liposomes in Tooth Socket Healing |
title_fullStr |
Effect of PDGF-BB, IGF-I Growth Factors and their Combination Carried by Liposomes in Tooth Socket Healing |
title_full_unstemmed |
Effect of PDGF-BB, IGF-I Growth Factors and their Combination Carried by Liposomes in Tooth Socket Healing |
title_sort |
Effect of PDGF-BB, IGF-I Growth Factors and their Combination Carried by Liposomes in Tooth Socket Healing |
author |
Abreu,Fernando Antonio Mauad de |
author_facet |
Abreu,Fernando Antonio Mauad de Ferreira,Cynthia Lopes Silva,Gerluza Aparecida Borges Paulo,Camila de Oliveira Miziara,Melissa Nunes Silveira,Frank Ferreira Alves,Jose Bento |
author_role |
author |
author2 |
Ferreira,Cynthia Lopes Silva,Gerluza Aparecida Borges Paulo,Camila de Oliveira Miziara,Melissa Nunes Silveira,Frank Ferreira Alves,Jose Bento |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Abreu,Fernando Antonio Mauad de Ferreira,Cynthia Lopes Silva,Gerluza Aparecida Borges Paulo,Camila de Oliveira Miziara,Melissa Nunes Silveira,Frank Ferreira Alves,Jose Bento |
dc.subject.por.fl_str_mv |
growth factor PDGF IGF liposome bone repair |
topic |
growth factor PDGF IGF liposome bone repair |
description |
This work evaluated the bone-forming potential of the platelet-derived growth factor isoform BB (PDGF-BB), insulin-like growth factor I (IGF-I), and mixed PDGF-BB/IGF-I delivered in liposomes compared with phosphate buffered saline (PBS), in the healing process of rat tooth sockets. One hundred and twelve Wistar rats were randomized into 7 groups of 16 animals each and were evaluated at 3, 7, 14 and 21 days after extraction of the maxillary second molars. The left sockets were treated with PBS (P), empty liposome (L), IGF-I in PBS (IP), IGF-I in liposome (IL), PDGF-BB in PBS (PDP), PDGF-BB in liposome (PDL) and both growth factors (GFs) together within liposomes (PDIL). The right sockets were filled with blood clot (BC). Histological and histomorphometric analyses were used to evaluate the formation of new bone and blood vessels. Immunohistochemistry was performed to evaluate the expression of osteocalcin and vascular endothelial growth factor (VEGF) during bone repair. Data were tested statistically using a Tukey's test according to a Dunn's analysis and Mann-Whitney U test followed by Kruskal-Wallis one-way analysis. Results were considered significant when p<0.05. A significantly higher percentage of bone trabeculae and a higher number of blood vessels were observed in the IL, PDL and PDIL groups (p<0.05). However, these GF-liposome groups had statistically similar results. Immunohistochemical assays first detected osteocalcin and VEGF expression at 3 days followed by a peak at 7 days. Lower immunoreactivity levels were observed in the BC, L, P, IP and PDP groups compared with the IL, PDL and PDIL groups (p<0.05). The results suggest that GFs carried by liposomes, either in isolated or mixed forms, enhanced the healing process in rat tooth sockets. The differential expression of the osteogenic markers VEGF and osteocalcin in the early phases of bone healing support these findings. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-08-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402013000400299 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402013000400299 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0103-6440201302238 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Fundação Odontológica de Ribeirão Preto |
publisher.none.fl_str_mv |
Fundação Odontológica de Ribeirão Preto |
dc.source.none.fl_str_mv |
Brazilian Dental Journal v.24 n.4 2013 reponame:Brazilian Dental Journal instname:Fundação Odontológica de Ribeirão Preto (FUNORP) instacron:FUNORP |
instname_str |
Fundação Odontológica de Ribeirão Preto (FUNORP) |
instacron_str |
FUNORP |
institution |
FUNORP |
reponame_str |
Brazilian Dental Journal |
collection |
Brazilian Dental Journal |
repository.name.fl_str_mv |
Brazilian Dental Journal - Fundação Odontológica de Ribeirão Preto (FUNORP) |
repository.mail.fl_str_mv |
bdj@forp.usp.br||sergio@fosjc.unesp.br |
_version_ |
1754204092660449280 |