Enamel Subsurface Caries-Like Lesions Induced in Human Teeth By Different Solutions: A TMR Analysis

Detalhes bibliográficos
Autor(a) principal: Gouvêa,Daiana Back
Data de Publicação: 2020
Outros Autores: Santos,Nicole Marchioro dos, Pessan,Juliano Pelim, Jardim,Juliana Jobim, Rodrigues,Jonas Almeida
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Dental Journal
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402020000200157
Resumo: Abstract This study assessed the effectiveness of models for developing subsurface caries lesions in vitro and verified mineral changes by transverse microradiography (TMR). Enamel blocks from permanent (n=5) and deciduous teeth (n=5) were submitted to lesion induction by immersion in demineralizing solutions during 96 h, followed by pH cycles of demineralization (de) and remineralization (re) for 10 days. Two de-/re solutions were tested. Demineralizing solution “A” was composed by 2.2 mM CaCl2, 2.2 mM KH2PO4, 0.05 M acetic acid, with pH 4.4 adjusted by 1 M KOH. Demineralizing solution “B” was composed by 2.2 mM CaCl2, 2.2 mM NaH2PO4, 0.05 M acetic acid and 0.25 ppmF, with pH 4.5 adjusted by 1M KOH. Solution “A” produced cavitated lesions in permanent teeth, whereas solution “B” led to subsurface lesions in deciduous teeth. Solution “B” was then tested in enamel blocks from permanent teeth (n=5) and subsurface lesions were obtained, so that solution “B” was employed for both substrates, and the blocks were treated with slurries of a fluoride dentifrice (1450 ppm F, as NaF, n=5) or a fluoride-free dentifrice (n=5). Solution “B” produced subsurface lesions in permanent and primary teeth of an average (±SD) depth of 88.4µm (±14.3) and 89.3µm (±15.8), respectively. TMR analysis demonstrated that lesions treated with fluoride-free dentifrice had significantly greater mineral loss. This study concluded that solution “B” developed subsurface lesions after pH cycling, and that mineral changes were successfully assessed by TMR.
id FUNORP-1_6ab4b0d1447e6c37e858cce1e2b9c387
oai_identifier_str oai:scielo:S0103-64402020000200157
network_acronym_str FUNORP-1
network_name_str Brazilian Dental Journal
repository_id_str
spelling Enamel Subsurface Caries-Like Lesions Induced in Human Teeth By Different Solutions: A TMR Analysisdental cariesremineralizationdemineralizationpH cyclingTMRAbstract This study assessed the effectiveness of models for developing subsurface caries lesions in vitro and verified mineral changes by transverse microradiography (TMR). Enamel blocks from permanent (n=5) and deciduous teeth (n=5) were submitted to lesion induction by immersion in demineralizing solutions during 96 h, followed by pH cycles of demineralization (de) and remineralization (re) for 10 days. Two de-/re solutions were tested. Demineralizing solution “A” was composed by 2.2 mM CaCl2, 2.2 mM KH2PO4, 0.05 M acetic acid, with pH 4.4 adjusted by 1 M KOH. Demineralizing solution “B” was composed by 2.2 mM CaCl2, 2.2 mM NaH2PO4, 0.05 M acetic acid and 0.25 ppmF, with pH 4.5 adjusted by 1M KOH. Solution “A” produced cavitated lesions in permanent teeth, whereas solution “B” led to subsurface lesions in deciduous teeth. Solution “B” was then tested in enamel blocks from permanent teeth (n=5) and subsurface lesions were obtained, so that solution “B” was employed for both substrates, and the blocks were treated with slurries of a fluoride dentifrice (1450 ppm F, as NaF, n=5) or a fluoride-free dentifrice (n=5). Solution “B” produced subsurface lesions in permanent and primary teeth of an average (±SD) depth of 88.4µm (±14.3) and 89.3µm (±15.8), respectively. TMR analysis demonstrated that lesions treated with fluoride-free dentifrice had significantly greater mineral loss. This study concluded that solution “B” developed subsurface lesions after pH cycling, and that mineral changes were successfully assessed by TMR.Fundação Odontológica de Ribeirão Preto2020-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402020000200157Brazilian Dental Journal v.31 n.2 2020reponame:Brazilian Dental Journalinstname:Fundação Odontológica de Ribeirão Preto (FUNORP)instacron:FUNORP10.1590/0103-6440202003123info:eu-repo/semantics/openAccessGouvêa,Daiana BackSantos,Nicole Marchioro dosPessan,Juliano PelimJardim,Juliana JobimRodrigues,Jonas Almeidaeng2020-06-10T00:00:00Zoai:scielo:S0103-64402020000200157Revistahttps://www.scielo.br/j/bdj/https://old.scielo.br/oai/scielo-oai.phpbdj@forp.usp.br||sergio@fosjc.unesp.br1806-47600103-6440opendoar:2020-06-10T00:00Brazilian Dental Journal - Fundação Odontológica de Ribeirão Preto (FUNORP)false
dc.title.none.fl_str_mv Enamel Subsurface Caries-Like Lesions Induced in Human Teeth By Different Solutions: A TMR Analysis
title Enamel Subsurface Caries-Like Lesions Induced in Human Teeth By Different Solutions: A TMR Analysis
spellingShingle Enamel Subsurface Caries-Like Lesions Induced in Human Teeth By Different Solutions: A TMR Analysis
Gouvêa,Daiana Back
dental caries
remineralization
demineralization
pH cycling
TMR
title_short Enamel Subsurface Caries-Like Lesions Induced in Human Teeth By Different Solutions: A TMR Analysis
title_full Enamel Subsurface Caries-Like Lesions Induced in Human Teeth By Different Solutions: A TMR Analysis
title_fullStr Enamel Subsurface Caries-Like Lesions Induced in Human Teeth By Different Solutions: A TMR Analysis
title_full_unstemmed Enamel Subsurface Caries-Like Lesions Induced in Human Teeth By Different Solutions: A TMR Analysis
title_sort Enamel Subsurface Caries-Like Lesions Induced in Human Teeth By Different Solutions: A TMR Analysis
author Gouvêa,Daiana Back
author_facet Gouvêa,Daiana Back
Santos,Nicole Marchioro dos
Pessan,Juliano Pelim
Jardim,Juliana Jobim
Rodrigues,Jonas Almeida
author_role author
author2 Santos,Nicole Marchioro dos
Pessan,Juliano Pelim
Jardim,Juliana Jobim
Rodrigues,Jonas Almeida
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Gouvêa,Daiana Back
Santos,Nicole Marchioro dos
Pessan,Juliano Pelim
Jardim,Juliana Jobim
Rodrigues,Jonas Almeida
dc.subject.por.fl_str_mv dental caries
remineralization
demineralization
pH cycling
TMR
topic dental caries
remineralization
demineralization
pH cycling
TMR
description Abstract This study assessed the effectiveness of models for developing subsurface caries lesions in vitro and verified mineral changes by transverse microradiography (TMR). Enamel blocks from permanent (n=5) and deciduous teeth (n=5) were submitted to lesion induction by immersion in demineralizing solutions during 96 h, followed by pH cycles of demineralization (de) and remineralization (re) for 10 days. Two de-/re solutions were tested. Demineralizing solution “A” was composed by 2.2 mM CaCl2, 2.2 mM KH2PO4, 0.05 M acetic acid, with pH 4.4 adjusted by 1 M KOH. Demineralizing solution “B” was composed by 2.2 mM CaCl2, 2.2 mM NaH2PO4, 0.05 M acetic acid and 0.25 ppmF, with pH 4.5 adjusted by 1M KOH. Solution “A” produced cavitated lesions in permanent teeth, whereas solution “B” led to subsurface lesions in deciduous teeth. Solution “B” was then tested in enamel blocks from permanent teeth (n=5) and subsurface lesions were obtained, so that solution “B” was employed for both substrates, and the blocks were treated with slurries of a fluoride dentifrice (1450 ppm F, as NaF, n=5) or a fluoride-free dentifrice (n=5). Solution “B” produced subsurface lesions in permanent and primary teeth of an average (±SD) depth of 88.4µm (±14.3) and 89.3µm (±15.8), respectively. TMR analysis demonstrated that lesions treated with fluoride-free dentifrice had significantly greater mineral loss. This study concluded that solution “B” developed subsurface lesions after pH cycling, and that mineral changes were successfully assessed by TMR.
publishDate 2020
dc.date.none.fl_str_mv 2020-04-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402020000200157
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402020000200157
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0103-6440202003123
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Fundação Odontológica de Ribeirão Preto
publisher.none.fl_str_mv Fundação Odontológica de Ribeirão Preto
dc.source.none.fl_str_mv Brazilian Dental Journal v.31 n.2 2020
reponame:Brazilian Dental Journal
instname:Fundação Odontológica de Ribeirão Preto (FUNORP)
instacron:FUNORP
instname_str Fundação Odontológica de Ribeirão Preto (FUNORP)
instacron_str FUNORP
institution FUNORP
reponame_str Brazilian Dental Journal
collection Brazilian Dental Journal
repository.name.fl_str_mv Brazilian Dental Journal - Fundação Odontológica de Ribeirão Preto (FUNORP)
repository.mail.fl_str_mv bdj@forp.usp.br||sergio@fosjc.unesp.br
_version_ 1754204095805128704