Analysis of the Epithelium-Mesenchymal Transition Process on Oral Squamous Cell Carcinomas
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Dental Journal |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402017000500543 |
Resumo: | Abstract The aim of this study is to evaluate the immunohistochemical expression of E-cadherin, N-cadherin and Bmi-1, and their association with clinical parameters and with the degree of histopathological differentiation in oral squamous cell carcinomas. 65 squamous cell carcinoma samples were used for constructing a tissue microarray block, and then immunohistochemistry was performed for different markers. A semi-quantitative analysis of the amount of positive tumor cells was performed by two blind and calibrated observers (Kappa>0.75). The statistical Mann-Whitney and Kruskal-Wallis tests were used to evaluate the data. The correlation between variables was investigated by the Spearman test, and the significance level set at p<0.05. We observed higher expression of Bmi-1 in tumors located in the palate (p<0.0001). In addition, poorly differentiated tumors had a greater amount of Bmi-1 positive cells (p=0.0011). Regarding the other correlations between variables, no significant associations were detected. In conclusion, poorly differentiated squamous cell carcinomas located in the palate have higher immunostaining of Bmi-1, which can characterize activation of the Epithelial-Mesenchymal Transition process in these tumors. |
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Brazilian Dental Journal |
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Analysis of the Epithelium-Mesenchymal Transition Process on Oral Squamous Cell Carcinomasoral cancerprognosisE-cadherinN-cadherinBmi-1.Abstract The aim of this study is to evaluate the immunohistochemical expression of E-cadherin, N-cadherin and Bmi-1, and their association with clinical parameters and with the degree of histopathological differentiation in oral squamous cell carcinomas. 65 squamous cell carcinoma samples were used for constructing a tissue microarray block, and then immunohistochemistry was performed for different markers. A semi-quantitative analysis of the amount of positive tumor cells was performed by two blind and calibrated observers (Kappa>0.75). The statistical Mann-Whitney and Kruskal-Wallis tests were used to evaluate the data. The correlation between variables was investigated by the Spearman test, and the significance level set at p<0.05. We observed higher expression of Bmi-1 in tumors located in the palate (p<0.0001). In addition, poorly differentiated tumors had a greater amount of Bmi-1 positive cells (p=0.0011). Regarding the other correlations between variables, no significant associations were detected. In conclusion, poorly differentiated squamous cell carcinomas located in the palate have higher immunostaining of Bmi-1, which can characterize activation of the Epithelial-Mesenchymal Transition process in these tumors.Fundação Odontológica de Ribeirão Preto2017-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402017000500543Brazilian Dental Journal v.28 n.5 2017reponame:Brazilian Dental Journalinstname:Fundação Odontológica de Ribeirão Preto (FUNORP)instacron:FUNORP10.1590/0103-6440201701484info:eu-repo/semantics/openAccessLima,Taiane Berguemaier deKlein,Isadora PeresOliveira,Márcia Gaiger deRados,Pantelis VarvakiSant’Ana Filho,ManoelVisioli,Fernandaeng2017-11-17T00:00:00Zoai:scielo:S0103-64402017000500543Revistahttps://www.scielo.br/j/bdj/https://old.scielo.br/oai/scielo-oai.phpbdj@forp.usp.br||sergio@fosjc.unesp.br1806-47600103-6440opendoar:2017-11-17T00:00Brazilian Dental Journal - Fundação Odontológica de Ribeirão Preto (FUNORP)false |
dc.title.none.fl_str_mv |
Analysis of the Epithelium-Mesenchymal Transition Process on Oral Squamous Cell Carcinomas |
title |
Analysis of the Epithelium-Mesenchymal Transition Process on Oral Squamous Cell Carcinomas |
spellingShingle |
Analysis of the Epithelium-Mesenchymal Transition Process on Oral Squamous Cell Carcinomas Lima,Taiane Berguemaier de oral cancer prognosis E-cadherin N-cadherin Bmi-1. |
title_short |
Analysis of the Epithelium-Mesenchymal Transition Process on Oral Squamous Cell Carcinomas |
title_full |
Analysis of the Epithelium-Mesenchymal Transition Process on Oral Squamous Cell Carcinomas |
title_fullStr |
Analysis of the Epithelium-Mesenchymal Transition Process on Oral Squamous Cell Carcinomas |
title_full_unstemmed |
Analysis of the Epithelium-Mesenchymal Transition Process on Oral Squamous Cell Carcinomas |
title_sort |
Analysis of the Epithelium-Mesenchymal Transition Process on Oral Squamous Cell Carcinomas |
author |
Lima,Taiane Berguemaier de |
author_facet |
Lima,Taiane Berguemaier de Klein,Isadora Peres Oliveira,Márcia Gaiger de Rados,Pantelis Varvaki Sant’Ana Filho,Manoel Visioli,Fernanda |
author_role |
author |
author2 |
Klein,Isadora Peres Oliveira,Márcia Gaiger de Rados,Pantelis Varvaki Sant’Ana Filho,Manoel Visioli,Fernanda |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Lima,Taiane Berguemaier de Klein,Isadora Peres Oliveira,Márcia Gaiger de Rados,Pantelis Varvaki Sant’Ana Filho,Manoel Visioli,Fernanda |
dc.subject.por.fl_str_mv |
oral cancer prognosis E-cadherin N-cadherin Bmi-1. |
topic |
oral cancer prognosis E-cadherin N-cadherin Bmi-1. |
description |
Abstract The aim of this study is to evaluate the immunohistochemical expression of E-cadherin, N-cadherin and Bmi-1, and their association with clinical parameters and with the degree of histopathological differentiation in oral squamous cell carcinomas. 65 squamous cell carcinoma samples were used for constructing a tissue microarray block, and then immunohistochemistry was performed for different markers. A semi-quantitative analysis of the amount of positive tumor cells was performed by two blind and calibrated observers (Kappa>0.75). The statistical Mann-Whitney and Kruskal-Wallis tests were used to evaluate the data. The correlation between variables was investigated by the Spearman test, and the significance level set at p<0.05. We observed higher expression of Bmi-1 in tumors located in the palate (p<0.0001). In addition, poorly differentiated tumors had a greater amount of Bmi-1 positive cells (p=0.0011). Regarding the other correlations between variables, no significant associations were detected. In conclusion, poorly differentiated squamous cell carcinomas located in the palate have higher immunostaining of Bmi-1, which can characterize activation of the Epithelial-Mesenchymal Transition process in these tumors. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402017000500543 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402017000500543 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0103-6440201701484 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Fundação Odontológica de Ribeirão Preto |
publisher.none.fl_str_mv |
Fundação Odontológica de Ribeirão Preto |
dc.source.none.fl_str_mv |
Brazilian Dental Journal v.28 n.5 2017 reponame:Brazilian Dental Journal instname:Fundação Odontológica de Ribeirão Preto (FUNORP) instacron:FUNORP |
instname_str |
Fundação Odontológica de Ribeirão Preto (FUNORP) |
instacron_str |
FUNORP |
institution |
FUNORP |
reponame_str |
Brazilian Dental Journal |
collection |
Brazilian Dental Journal |
repository.name.fl_str_mv |
Brazilian Dental Journal - Fundação Odontológica de Ribeirão Preto (FUNORP) |
repository.mail.fl_str_mv |
bdj@forp.usp.br||sergio@fosjc.unesp.br |
_version_ |
1754204094844633088 |