Physicochemical and Biological Evaluation of Endodontic Filling Materials for Primary Teeth

Detalhes bibliográficos
Autor(a) principal: Pilownic,Katerine Jahnecke
Data de Publicação: 2017
Outros Autores: Gomes,Ana Paula Neutzling, Wang,Zhe Jun, Almeida,Luiza Helena Silva, Romano,Ana Regina, Shen,Ya, Felix,Anelize de Oliveira Campello, Haapasalo,Markus, Pappen,Fernanda Geraldo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Dental Journal
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402017000500578
Resumo: Abstract This study assessed the pH, radiopacity, antimicrobial effect, cytotoxicity and biocompatibility of endodontic filling materials for primary teeth. Zinc oxide eugenol (ZOE), Vitapex and Calen paste thickened with zinc oxide (ZO) were evaluated in comparison to an experimental MTA-based material. Radiopacity was tested using a graduated aluminum stepwedge with a digital sensor (n=5). The materials pH was recorded at 1, 4, 12 h; 1, 3, 7, 15 and 30 days (n=5). Direct contact test was used to assess the antimicrobial efficacy against Enterococcus faecalis after 1, 4, 12, 24 h (n=5). Cytotoxicity assay used MTT test for cell viability after incubation for 1, 3 and 7 days (n=5). For biocompatibility test, Wistar rats had received implants containing each material (n=5). The biopsied tissues were histologically analyzed after 15, 30 and 60 days. The results of radiopacity, pH, antimicrobial capacity and cytotoxicity were analyzed using ANOVA and Tukey tests. The histological data were submitted to Kruskal-Wallis test. The experimental material presented the lowest radiopacity (3.28 mm Al) and had a pH>12.0 throughout the test period. The experimental material showed the highest antibacterial effect, killing over 99.97% bacteria in 4 h. Vitapex presented the highest cell viability. Initially, biocompatibility test showed moderate to severe inflammation in all groups. After 60 days, Calen+ZO group showed moderate inflammation, while the others showed predominantly mild inflammatory reaction. The present results demonstrated that the experimental MTA-based material exhibited satisfactory behavior regarding the studied properties. Additional in vivo studies are necessary for a better evaluation of the material.
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spelling Physicochemical and Biological Evaluation of Endodontic Filling Materials for Primary Teethendodonticsdeciduous toothroot canal filling materials.Abstract This study assessed the pH, radiopacity, antimicrobial effect, cytotoxicity and biocompatibility of endodontic filling materials for primary teeth. Zinc oxide eugenol (ZOE), Vitapex and Calen paste thickened with zinc oxide (ZO) were evaluated in comparison to an experimental MTA-based material. Radiopacity was tested using a graduated aluminum stepwedge with a digital sensor (n=5). The materials pH was recorded at 1, 4, 12 h; 1, 3, 7, 15 and 30 days (n=5). Direct contact test was used to assess the antimicrobial efficacy against Enterococcus faecalis after 1, 4, 12, 24 h (n=5). Cytotoxicity assay used MTT test for cell viability after incubation for 1, 3 and 7 days (n=5). For biocompatibility test, Wistar rats had received implants containing each material (n=5). The biopsied tissues were histologically analyzed after 15, 30 and 60 days. The results of radiopacity, pH, antimicrobial capacity and cytotoxicity were analyzed using ANOVA and Tukey tests. The histological data were submitted to Kruskal-Wallis test. The experimental material presented the lowest radiopacity (3.28 mm Al) and had a pH>12.0 throughout the test period. The experimental material showed the highest antibacterial effect, killing over 99.97% bacteria in 4 h. Vitapex presented the highest cell viability. Initially, biocompatibility test showed moderate to severe inflammation in all groups. After 60 days, Calen+ZO group showed moderate inflammation, while the others showed predominantly mild inflammatory reaction. The present results demonstrated that the experimental MTA-based material exhibited satisfactory behavior regarding the studied properties. Additional in vivo studies are necessary for a better evaluation of the material.Fundação Odontológica de Ribeirão Preto2017-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402017000500578Brazilian Dental Journal v.28 n.5 2017reponame:Brazilian Dental Journalinstname:Fundação Odontológica de Ribeirão Preto (FUNORP)instacron:FUNORP10.1590/0103-6440201701573info:eu-repo/semantics/openAccessPilownic,Katerine JahneckeGomes,Ana Paula NeutzlingWang,Zhe JunAlmeida,Luiza Helena SilvaRomano,Ana ReginaShen,YaFelix,Anelize de Oliveira CampelloHaapasalo,MarkusPappen,Fernanda Geraldoeng2017-11-17T00:00:00Zoai:scielo:S0103-64402017000500578Revistahttps://www.scielo.br/j/bdj/https://old.scielo.br/oai/scielo-oai.phpbdj@forp.usp.br||sergio@fosjc.unesp.br1806-47600103-6440opendoar:2017-11-17T00:00Brazilian Dental Journal - Fundação Odontológica de Ribeirão Preto (FUNORP)false
dc.title.none.fl_str_mv Physicochemical and Biological Evaluation of Endodontic Filling Materials for Primary Teeth
title Physicochemical and Biological Evaluation of Endodontic Filling Materials for Primary Teeth
spellingShingle Physicochemical and Biological Evaluation of Endodontic Filling Materials for Primary Teeth
Pilownic,Katerine Jahnecke
endodontics
deciduous tooth
root canal filling materials.
title_short Physicochemical and Biological Evaluation of Endodontic Filling Materials for Primary Teeth
title_full Physicochemical and Biological Evaluation of Endodontic Filling Materials for Primary Teeth
title_fullStr Physicochemical and Biological Evaluation of Endodontic Filling Materials for Primary Teeth
title_full_unstemmed Physicochemical and Biological Evaluation of Endodontic Filling Materials for Primary Teeth
title_sort Physicochemical and Biological Evaluation of Endodontic Filling Materials for Primary Teeth
author Pilownic,Katerine Jahnecke
author_facet Pilownic,Katerine Jahnecke
Gomes,Ana Paula Neutzling
Wang,Zhe Jun
Almeida,Luiza Helena Silva
Romano,Ana Regina
Shen,Ya
Felix,Anelize de Oliveira Campello
Haapasalo,Markus
Pappen,Fernanda Geraldo
author_role author
author2 Gomes,Ana Paula Neutzling
Wang,Zhe Jun
Almeida,Luiza Helena Silva
Romano,Ana Regina
Shen,Ya
Felix,Anelize de Oliveira Campello
Haapasalo,Markus
Pappen,Fernanda Geraldo
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Pilownic,Katerine Jahnecke
Gomes,Ana Paula Neutzling
Wang,Zhe Jun
Almeida,Luiza Helena Silva
Romano,Ana Regina
Shen,Ya
Felix,Anelize de Oliveira Campello
Haapasalo,Markus
Pappen,Fernanda Geraldo
dc.subject.por.fl_str_mv endodontics
deciduous tooth
root canal filling materials.
topic endodontics
deciduous tooth
root canal filling materials.
description Abstract This study assessed the pH, radiopacity, antimicrobial effect, cytotoxicity and biocompatibility of endodontic filling materials for primary teeth. Zinc oxide eugenol (ZOE), Vitapex and Calen paste thickened with zinc oxide (ZO) were evaluated in comparison to an experimental MTA-based material. Radiopacity was tested using a graduated aluminum stepwedge with a digital sensor (n=5). The materials pH was recorded at 1, 4, 12 h; 1, 3, 7, 15 and 30 days (n=5). Direct contact test was used to assess the antimicrobial efficacy against Enterococcus faecalis after 1, 4, 12, 24 h (n=5). Cytotoxicity assay used MTT test for cell viability after incubation for 1, 3 and 7 days (n=5). For biocompatibility test, Wistar rats had received implants containing each material (n=5). The biopsied tissues were histologically analyzed after 15, 30 and 60 days. The results of radiopacity, pH, antimicrobial capacity and cytotoxicity were analyzed using ANOVA and Tukey tests. The histological data were submitted to Kruskal-Wallis test. The experimental material presented the lowest radiopacity (3.28 mm Al) and had a pH>12.0 throughout the test period. The experimental material showed the highest antibacterial effect, killing over 99.97% bacteria in 4 h. Vitapex presented the highest cell viability. Initially, biocompatibility test showed moderate to severe inflammation in all groups. After 60 days, Calen+ZO group showed moderate inflammation, while the others showed predominantly mild inflammatory reaction. The present results demonstrated that the experimental MTA-based material exhibited satisfactory behavior regarding the studied properties. Additional in vivo studies are necessary for a better evaluation of the material.
publishDate 2017
dc.date.none.fl_str_mv 2017-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402017000500578
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402017000500578
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0103-6440201701573
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Fundação Odontológica de Ribeirão Preto
publisher.none.fl_str_mv Fundação Odontológica de Ribeirão Preto
dc.source.none.fl_str_mv Brazilian Dental Journal v.28 n.5 2017
reponame:Brazilian Dental Journal
instname:Fundação Odontológica de Ribeirão Preto (FUNORP)
instacron:FUNORP
instname_str Fundação Odontológica de Ribeirão Preto (FUNORP)
instacron_str FUNORP
institution FUNORP
reponame_str Brazilian Dental Journal
collection Brazilian Dental Journal
repository.name.fl_str_mv Brazilian Dental Journal - Fundação Odontológica de Ribeirão Preto (FUNORP)
repository.mail.fl_str_mv bdj@forp.usp.br||sergio@fosjc.unesp.br
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