Phosphoric acid containing proanthocyanidin enhances bond stability of resin/dentin interface
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Dental Journal |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402022000400062 |
Resumo: | Abstract Proanthocyanidin (PA) is a promising dentin biomodifier due to its ability to stabilize collagen fibrils against degradation by matrix metalloproteinases (MMPs); however, the most effective protocol to incorporate PA into bonding procedures is still unclear. This study evaluated the effect of dentin biomodification with a PA acid etchant on MMP activity, adhesive interface morphology and resin-dentin microtensile bond strength. Sound extracted human molars were flattened to expose dentin and acid-etched for 15 s according to the groups: EXP - experimental phosphoric acid; EXP+PA - experimental phosphoric acid 10% PA; TE - total-etching system; SE - self-etching system. Samples were restored with composite resin and stored in distilled water (37ºC). MMP activity and interface morphology were analyzed after 24 h by in situ zymography (n=6) and scanning electron microscopy (n=3), respectively. The resin-dentin microtensile bond strength (μTBS) was evaluated after 24 h and 6 months storage (n=6). Significantly higher MMP activity was detected in etched dentin compared with untreated dentin (p<0.05), but no difference among acid groups was found. Resin tags and microtags, indicative of proper adhesive system penetration in dentinal tubules and microtubules, were observed along the hybrid layer in all groups. There was no difference in μTBS between 24 h and 6 months for EXP+PA; moreover, it showed higher long-term μTBS compared with TE and EXP (p<0.05). The results suggest that 15 s of biomodification was not sufficient to significantly reduce MMP activity; nonetheless, EXP+PA was still able to improve resin-dentin bond stability compared with total- and self-etching commercial systems. |
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Brazilian Dental Journal |
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Phosphoric acid containing proanthocyanidin enhances bond stability of resin/dentin interfaceDentin biomodificationmatrix metalloproteinasesin situ zymographymicrotensile bond strengthadhesive interfaceAbstract Proanthocyanidin (PA) is a promising dentin biomodifier due to its ability to stabilize collagen fibrils against degradation by matrix metalloproteinases (MMPs); however, the most effective protocol to incorporate PA into bonding procedures is still unclear. This study evaluated the effect of dentin biomodification with a PA acid etchant on MMP activity, adhesive interface morphology and resin-dentin microtensile bond strength. Sound extracted human molars were flattened to expose dentin and acid-etched for 15 s according to the groups: EXP - experimental phosphoric acid; EXP+PA - experimental phosphoric acid 10% PA; TE - total-etching system; SE - self-etching system. Samples were restored with composite resin and stored in distilled water (37ºC). MMP activity and interface morphology were analyzed after 24 h by in situ zymography (n=6) and scanning electron microscopy (n=3), respectively. The resin-dentin microtensile bond strength (μTBS) was evaluated after 24 h and 6 months storage (n=6). Significantly higher MMP activity was detected in etched dentin compared with untreated dentin (p<0.05), but no difference among acid groups was found. Resin tags and microtags, indicative of proper adhesive system penetration in dentinal tubules and microtubules, were observed along the hybrid layer in all groups. There was no difference in μTBS between 24 h and 6 months for EXP+PA; moreover, it showed higher long-term μTBS compared with TE and EXP (p<0.05). The results suggest that 15 s of biomodification was not sufficient to significantly reduce MMP activity; nonetheless, EXP+PA was still able to improve resin-dentin bond stability compared with total- and self-etching commercial systems.Fundação Odontológica de Ribeirão Preto2022-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402022000400062Brazilian Dental Journal v.33 n.4 2022reponame:Brazilian Dental Journalinstname:Fundação Odontológica de Ribeirão Preto (FUNORP)instacron:FUNORP10.1590/0103-6440202203941info:eu-repo/semantics/openAccessRey,Yumi C. DelPalma-Dibb,Regina G.França,RodrigoPaula-Silva,Francisco W.G.Guedes,Débora F.C.Fiuza,CristinaFernandes,Ana C.B.C.J.Faraoni,Juliana J.Roselino,Lourenço M.R.eng2022-08-23T00:00:00Zoai:scielo:S0103-64402022000400062Revistahttps://www.scielo.br/j/bdj/https://old.scielo.br/oai/scielo-oai.phpbdj@forp.usp.br||sergio@fosjc.unesp.br1806-47600103-6440opendoar:2022-08-23T00:00Brazilian Dental Journal - Fundação Odontológica de Ribeirão Preto (FUNORP)false |
dc.title.none.fl_str_mv |
Phosphoric acid containing proanthocyanidin enhances bond stability of resin/dentin interface |
title |
Phosphoric acid containing proanthocyanidin enhances bond stability of resin/dentin interface |
spellingShingle |
Phosphoric acid containing proanthocyanidin enhances bond stability of resin/dentin interface Rey,Yumi C. Del Dentin biomodification matrix metalloproteinases in situ zymography microtensile bond strength adhesive interface |
title_short |
Phosphoric acid containing proanthocyanidin enhances bond stability of resin/dentin interface |
title_full |
Phosphoric acid containing proanthocyanidin enhances bond stability of resin/dentin interface |
title_fullStr |
Phosphoric acid containing proanthocyanidin enhances bond stability of resin/dentin interface |
title_full_unstemmed |
Phosphoric acid containing proanthocyanidin enhances bond stability of resin/dentin interface |
title_sort |
Phosphoric acid containing proanthocyanidin enhances bond stability of resin/dentin interface |
author |
Rey,Yumi C. Del |
author_facet |
Rey,Yumi C. Del Palma-Dibb,Regina G. França,Rodrigo Paula-Silva,Francisco W.G. Guedes,Débora F.C. Fiuza,Cristina Fernandes,Ana C.B.C.J. Faraoni,Juliana J. Roselino,Lourenço M.R. |
author_role |
author |
author2 |
Palma-Dibb,Regina G. França,Rodrigo Paula-Silva,Francisco W.G. Guedes,Débora F.C. Fiuza,Cristina Fernandes,Ana C.B.C.J. Faraoni,Juliana J. Roselino,Lourenço M.R. |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Rey,Yumi C. Del Palma-Dibb,Regina G. França,Rodrigo Paula-Silva,Francisco W.G. Guedes,Débora F.C. Fiuza,Cristina Fernandes,Ana C.B.C.J. Faraoni,Juliana J. Roselino,Lourenço M.R. |
dc.subject.por.fl_str_mv |
Dentin biomodification matrix metalloproteinases in situ zymography microtensile bond strength adhesive interface |
topic |
Dentin biomodification matrix metalloproteinases in situ zymography microtensile bond strength adhesive interface |
description |
Abstract Proanthocyanidin (PA) is a promising dentin biomodifier due to its ability to stabilize collagen fibrils against degradation by matrix metalloproteinases (MMPs); however, the most effective protocol to incorporate PA into bonding procedures is still unclear. This study evaluated the effect of dentin biomodification with a PA acid etchant on MMP activity, adhesive interface morphology and resin-dentin microtensile bond strength. Sound extracted human molars were flattened to expose dentin and acid-etched for 15 s according to the groups: EXP - experimental phosphoric acid; EXP+PA - experimental phosphoric acid 10% PA; TE - total-etching system; SE - self-etching system. Samples were restored with composite resin and stored in distilled water (37ºC). MMP activity and interface morphology were analyzed after 24 h by in situ zymography (n=6) and scanning electron microscopy (n=3), respectively. The resin-dentin microtensile bond strength (μTBS) was evaluated after 24 h and 6 months storage (n=6). Significantly higher MMP activity was detected in etched dentin compared with untreated dentin (p<0.05), but no difference among acid groups was found. Resin tags and microtags, indicative of proper adhesive system penetration in dentinal tubules and microtubules, were observed along the hybrid layer in all groups. There was no difference in μTBS between 24 h and 6 months for EXP+PA; moreover, it showed higher long-term μTBS compared with TE and EXP (p<0.05). The results suggest that 15 s of biomodification was not sufficient to significantly reduce MMP activity; nonetheless, EXP+PA was still able to improve resin-dentin bond stability compared with total- and self-etching commercial systems. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-08-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402022000400062 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402022000400062 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0103-6440202203941 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Fundação Odontológica de Ribeirão Preto |
publisher.none.fl_str_mv |
Fundação Odontológica de Ribeirão Preto |
dc.source.none.fl_str_mv |
Brazilian Dental Journal v.33 n.4 2022 reponame:Brazilian Dental Journal instname:Fundação Odontológica de Ribeirão Preto (FUNORP) instacron:FUNORP |
instname_str |
Fundação Odontológica de Ribeirão Preto (FUNORP) |
instacron_str |
FUNORP |
institution |
FUNORP |
reponame_str |
Brazilian Dental Journal |
collection |
Brazilian Dental Journal |
repository.name.fl_str_mv |
Brazilian Dental Journal - Fundação Odontológica de Ribeirão Preto (FUNORP) |
repository.mail.fl_str_mv |
bdj@forp.usp.br||sergio@fosjc.unesp.br |
_version_ |
1754204096671252480 |