Effects of Papain-Based Gel Used For Caries Removal on Macrophages and Dental Pulp Cells
Autor(a) principal: | |
---|---|
Data de Publicação: | 2019 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Dental Journal |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402019000500484 |
Resumo: | Abstract Papain-based gel is used for chemical-mechanical caries removal and present antimicrobial and anti-inflammatory activities. However, its effects on dental pulp cells and on macrophages remains largely unknown. Therefore, the aim of this study was to investigate whether the papain-based gel Papacárie Duo® acts as an immunomodulator in lipopolysaccharide (LPS)-activated macrophages and its effects on dental pulp cells . J774.1 macrophage and OD-21 dental pulp cells were stimulated with 0.5% and 5% of Papacárie Duo®, following pre-treatment or not with LPS. After 24 h, a lactate dehydrogenase assay was used to measure cytotoxicity, a tetrazolium-based colorimetric assay (MTT) was used to measure cell viability, and qRT-PCR was used to analyze relative gene expression of Ptgs2, Il10, Tnf, Mmp9, Runx2, Ibsp and Spp1. Papacárie Duo® was cytotoxic and reduced cell viability at 5% but not at 0.5% in both cultures. In macrophages, Papacárie Duo® increased the expression Il10 and LPS-induced Ptgs2, but it did not affect Tnf or Mmp9. In OD-21 cells, Papacárie Duo® inhibited Runx2 and Ibsp expression, but stimulated Spp1 expression. Papain-based gel presented a concentration dependent cytotoxicity, without affecting cell viability, for dental pulp cells and macrophages. Interestingly, the gel presented an inhibitory effect on pulp cell differentiation but modulated the activation of macrophages stimulated with LPS. We speculate that in dental pulp tissue, Papacárie Duo® would impair reparative dentinogenesis but could activate macrophages to perform their role in defense and inflammation. |
id |
FUNORP-1_9dfea413d2ab1f4473ba68be60103f63 |
---|---|
oai_identifier_str |
oai:scielo:S0103-64402019000500484 |
network_acronym_str |
FUNORP-1 |
network_name_str |
Brazilian Dental Journal |
repository_id_str |
|
spelling |
Effects of Papain-Based Gel Used For Caries Removal on Macrophages and Dental Pulp Cellspapain-based gelmacrophagesdental pulp cellschemo-mechanical caries removalAbstract Papain-based gel is used for chemical-mechanical caries removal and present antimicrobial and anti-inflammatory activities. However, its effects on dental pulp cells and on macrophages remains largely unknown. Therefore, the aim of this study was to investigate whether the papain-based gel Papacárie Duo® acts as an immunomodulator in lipopolysaccharide (LPS)-activated macrophages and its effects on dental pulp cells . J774.1 macrophage and OD-21 dental pulp cells were stimulated with 0.5% and 5% of Papacárie Duo®, following pre-treatment or not with LPS. After 24 h, a lactate dehydrogenase assay was used to measure cytotoxicity, a tetrazolium-based colorimetric assay (MTT) was used to measure cell viability, and qRT-PCR was used to analyze relative gene expression of Ptgs2, Il10, Tnf, Mmp9, Runx2, Ibsp and Spp1. Papacárie Duo® was cytotoxic and reduced cell viability at 5% but not at 0.5% in both cultures. In macrophages, Papacárie Duo® increased the expression Il10 and LPS-induced Ptgs2, but it did not affect Tnf or Mmp9. In OD-21 cells, Papacárie Duo® inhibited Runx2 and Ibsp expression, but stimulated Spp1 expression. Papain-based gel presented a concentration dependent cytotoxicity, without affecting cell viability, for dental pulp cells and macrophages. Interestingly, the gel presented an inhibitory effect on pulp cell differentiation but modulated the activation of macrophages stimulated with LPS. We speculate that in dental pulp tissue, Papacárie Duo® would impair reparative dentinogenesis but could activate macrophages to perform their role in defense and inflammation.Fundação Odontológica de Ribeirão Preto2019-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402019000500484Brazilian Dental Journal v.30 n.5 2019reponame:Brazilian Dental Journalinstname:Fundação Odontológica de Ribeirão Preto (FUNORP)instacron:FUNORP10.1590/0103-6440201902560info:eu-repo/semantics/openAccessBastos,Laura AlvesSilva,Francine LorencettiThomé,João Pedro de QueirozArnez,Maya Fernanda ManfrinFaccioli,Lúcia HelenaPaula-Silva,Francisco Wanderley Garciaeng2019-10-23T00:00:00Zoai:scielo:S0103-64402019000500484Revistahttps://www.scielo.br/j/bdj/https://old.scielo.br/oai/scielo-oai.phpbdj@forp.usp.br||sergio@fosjc.unesp.br1806-47600103-6440opendoar:2019-10-23T00:00Brazilian Dental Journal - Fundação Odontológica de Ribeirão Preto (FUNORP)false |
dc.title.none.fl_str_mv |
Effects of Papain-Based Gel Used For Caries Removal on Macrophages and Dental Pulp Cells |
title |
Effects of Papain-Based Gel Used For Caries Removal on Macrophages and Dental Pulp Cells |
spellingShingle |
Effects of Papain-Based Gel Used For Caries Removal on Macrophages and Dental Pulp Cells Bastos,Laura Alves papain-based gel macrophages dental pulp cells chemo-mechanical caries removal |
title_short |
Effects of Papain-Based Gel Used For Caries Removal on Macrophages and Dental Pulp Cells |
title_full |
Effects of Papain-Based Gel Used For Caries Removal on Macrophages and Dental Pulp Cells |
title_fullStr |
Effects of Papain-Based Gel Used For Caries Removal on Macrophages and Dental Pulp Cells |
title_full_unstemmed |
Effects of Papain-Based Gel Used For Caries Removal on Macrophages and Dental Pulp Cells |
title_sort |
Effects of Papain-Based Gel Used For Caries Removal on Macrophages and Dental Pulp Cells |
author |
Bastos,Laura Alves |
author_facet |
Bastos,Laura Alves Silva,Francine Lorencetti Thomé,João Pedro de Queiroz Arnez,Maya Fernanda Manfrin Faccioli,Lúcia Helena Paula-Silva,Francisco Wanderley Garcia |
author_role |
author |
author2 |
Silva,Francine Lorencetti Thomé,João Pedro de Queiroz Arnez,Maya Fernanda Manfrin Faccioli,Lúcia Helena Paula-Silva,Francisco Wanderley Garcia |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Bastos,Laura Alves Silva,Francine Lorencetti Thomé,João Pedro de Queiroz Arnez,Maya Fernanda Manfrin Faccioli,Lúcia Helena Paula-Silva,Francisco Wanderley Garcia |
dc.subject.por.fl_str_mv |
papain-based gel macrophages dental pulp cells chemo-mechanical caries removal |
topic |
papain-based gel macrophages dental pulp cells chemo-mechanical caries removal |
description |
Abstract Papain-based gel is used for chemical-mechanical caries removal and present antimicrobial and anti-inflammatory activities. However, its effects on dental pulp cells and on macrophages remains largely unknown. Therefore, the aim of this study was to investigate whether the papain-based gel Papacárie Duo® acts as an immunomodulator in lipopolysaccharide (LPS)-activated macrophages and its effects on dental pulp cells . J774.1 macrophage and OD-21 dental pulp cells were stimulated with 0.5% and 5% of Papacárie Duo®, following pre-treatment or not with LPS. After 24 h, a lactate dehydrogenase assay was used to measure cytotoxicity, a tetrazolium-based colorimetric assay (MTT) was used to measure cell viability, and qRT-PCR was used to analyze relative gene expression of Ptgs2, Il10, Tnf, Mmp9, Runx2, Ibsp and Spp1. Papacárie Duo® was cytotoxic and reduced cell viability at 5% but not at 0.5% in both cultures. In macrophages, Papacárie Duo® increased the expression Il10 and LPS-induced Ptgs2, but it did not affect Tnf or Mmp9. In OD-21 cells, Papacárie Duo® inhibited Runx2 and Ibsp expression, but stimulated Spp1 expression. Papain-based gel presented a concentration dependent cytotoxicity, without affecting cell viability, for dental pulp cells and macrophages. Interestingly, the gel presented an inhibitory effect on pulp cell differentiation but modulated the activation of macrophages stimulated with LPS. We speculate that in dental pulp tissue, Papacárie Duo® would impair reparative dentinogenesis but could activate macrophages to perform their role in defense and inflammation. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402019000500484 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402019000500484 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0103-6440201902560 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Fundação Odontológica de Ribeirão Preto |
publisher.none.fl_str_mv |
Fundação Odontológica de Ribeirão Preto |
dc.source.none.fl_str_mv |
Brazilian Dental Journal v.30 n.5 2019 reponame:Brazilian Dental Journal instname:Fundação Odontológica de Ribeirão Preto (FUNORP) instacron:FUNORP |
instname_str |
Fundação Odontológica de Ribeirão Preto (FUNORP) |
instacron_str |
FUNORP |
institution |
FUNORP |
reponame_str |
Brazilian Dental Journal |
collection |
Brazilian Dental Journal |
repository.name.fl_str_mv |
Brazilian Dental Journal - Fundação Odontológica de Ribeirão Preto (FUNORP) |
repository.mail.fl_str_mv |
bdj@forp.usp.br||sergio@fosjc.unesp.br |
_version_ |
1754204095725436928 |