Antibiofilm Activity of an Experimental Ricinus Communis Dentifrice on Soft Denture Liners

Detalhes bibliográficos
Autor(a) principal: Badaró,Maurício Malheiros
Data de Publicação: 2019
Outros Autores: Leite-Fernandes,Vanessa Maria Fagundes, Martin,Luciano Trevisan, Oliveira,Viviane de Cássia, Watanabe,Evandro, Paranhos,Helena de Freitas de Oliveira, Silva-Lovato,Cláudia Helena
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Dental Journal
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402019000300252
Resumo: Abstract The disadvantage of liners materials is the difficulty of biofilm control. It was compared an experimental dentifrice contained Ricinus communis, with commercials dentifrices as antibiofilm activity against microorganisms on denture liner. Six hundred specimens were distributed in 5 groups (n=18/ microorganism): water; experimental dentifrice; specific dentifrice for denture and two conventional dentifrices against C. albicans; C. glabrata; S. mutans; S. aureus; E. coli. Each group had a negative (n=5; without contamination) and positive control (n=15/ microorganism; without cleaning). The antibiofilm activity was evaluated by the method of biofilm formation in triplicate. The specimens were contaminated in a standard way and incubated. After that, manual brushing was performed (60 s), washed with PBS, immersed in liquid culture medium for resuspension and sowing in solid medium. The results (mean of triplicates) were expressed in CFU/mL. The data was submitted to Shapiro-Wilk, ANOVA and Tukey test (p<0.05). The specific dentifrice (1.27±1.20) was the most effective against S. mutans, followed by conventional (Trihydral, 3.13±0.88; Colgate, 2.16±2.02) and experimental (3.81±1.37) dentifrices, which were similar to each other (p=0.008). All of them were different from water (4.79±1.42). The specific (0.21±0.21) and experimental (0.36±0.25) dentifrices were similar against S. aureus, with a higher mean of CFU when compared to conventional (Colgate, 0.06±0.13), which was more efficient (p=0.000). For C. albicans, C. glabrata and E. coli, all dentifrices were similar to water (p=0.186). It was concluded, that the experimental dentifrice was effective against S. aureus and had not efficacy against Candida spp.; S. mutans; E. coli, as occurred with the commercials dentifrices.
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spelling Antibiofilm Activity of an Experimental Ricinus Communis Dentifrice on Soft Denture Linersdenture linersdenture cleansersdenturesdentifricescastor beanAbstract The disadvantage of liners materials is the difficulty of biofilm control. It was compared an experimental dentifrice contained Ricinus communis, with commercials dentifrices as antibiofilm activity against microorganisms on denture liner. Six hundred specimens were distributed in 5 groups (n=18/ microorganism): water; experimental dentifrice; specific dentifrice for denture and two conventional dentifrices against C. albicans; C. glabrata; S. mutans; S. aureus; E. coli. Each group had a negative (n=5; without contamination) and positive control (n=15/ microorganism; without cleaning). The antibiofilm activity was evaluated by the method of biofilm formation in triplicate. The specimens were contaminated in a standard way and incubated. After that, manual brushing was performed (60 s), washed with PBS, immersed in liquid culture medium for resuspension and sowing in solid medium. The results (mean of triplicates) were expressed in CFU/mL. The data was submitted to Shapiro-Wilk, ANOVA and Tukey test (p<0.05). The specific dentifrice (1.27±1.20) was the most effective against S. mutans, followed by conventional (Trihydral, 3.13±0.88; Colgate, 2.16±2.02) and experimental (3.81±1.37) dentifrices, which were similar to each other (p=0.008). All of them were different from water (4.79±1.42). The specific (0.21±0.21) and experimental (0.36±0.25) dentifrices were similar against S. aureus, with a higher mean of CFU when compared to conventional (Colgate, 0.06±0.13), which was more efficient (p=0.000). For C. albicans, C. glabrata and E. coli, all dentifrices were similar to water (p=0.186). It was concluded, that the experimental dentifrice was effective against S. aureus and had not efficacy against Candida spp.; S. mutans; E. coli, as occurred with the commercials dentifrices.Fundação Odontológica de Ribeirão Preto2019-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402019000300252Brazilian Dental Journal v.30 n.3 2019reponame:Brazilian Dental Journalinstname:Fundação Odontológica de Ribeirão Preto (FUNORP)instacron:FUNORP10.1590/0103-6440201902326info:eu-repo/semantics/openAccessBadaró,Maurício MalheirosLeite-Fernandes,Vanessa Maria FagundesMartin,Luciano TrevisanOliveira,Viviane de CássiaWatanabe,EvandroParanhos,Helena de Freitas de OliveiraSilva-Lovato,Cláudia Helenaeng2019-06-06T00:00:00Zoai:scielo:S0103-64402019000300252Revistahttps://www.scielo.br/j/bdj/https://old.scielo.br/oai/scielo-oai.phpbdj@forp.usp.br||sergio@fosjc.unesp.br1806-47600103-6440opendoar:2019-06-06T00:00Brazilian Dental Journal - Fundação Odontológica de Ribeirão Preto (FUNORP)false
dc.title.none.fl_str_mv Antibiofilm Activity of an Experimental Ricinus Communis Dentifrice on Soft Denture Liners
title Antibiofilm Activity of an Experimental Ricinus Communis Dentifrice on Soft Denture Liners
spellingShingle Antibiofilm Activity of an Experimental Ricinus Communis Dentifrice on Soft Denture Liners
Badaró,Maurício Malheiros
denture liners
denture cleansers
dentures
dentifrices
castor bean
title_short Antibiofilm Activity of an Experimental Ricinus Communis Dentifrice on Soft Denture Liners
title_full Antibiofilm Activity of an Experimental Ricinus Communis Dentifrice on Soft Denture Liners
title_fullStr Antibiofilm Activity of an Experimental Ricinus Communis Dentifrice on Soft Denture Liners
title_full_unstemmed Antibiofilm Activity of an Experimental Ricinus Communis Dentifrice on Soft Denture Liners
title_sort Antibiofilm Activity of an Experimental Ricinus Communis Dentifrice on Soft Denture Liners
author Badaró,Maurício Malheiros
author_facet Badaró,Maurício Malheiros
Leite-Fernandes,Vanessa Maria Fagundes
Martin,Luciano Trevisan
Oliveira,Viviane de Cássia
Watanabe,Evandro
Paranhos,Helena de Freitas de Oliveira
Silva-Lovato,Cláudia Helena
author_role author
author2 Leite-Fernandes,Vanessa Maria Fagundes
Martin,Luciano Trevisan
Oliveira,Viviane de Cássia
Watanabe,Evandro
Paranhos,Helena de Freitas de Oliveira
Silva-Lovato,Cláudia Helena
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Badaró,Maurício Malheiros
Leite-Fernandes,Vanessa Maria Fagundes
Martin,Luciano Trevisan
Oliveira,Viviane de Cássia
Watanabe,Evandro
Paranhos,Helena de Freitas de Oliveira
Silva-Lovato,Cláudia Helena
dc.subject.por.fl_str_mv denture liners
denture cleansers
dentures
dentifrices
castor bean
topic denture liners
denture cleansers
dentures
dentifrices
castor bean
description Abstract The disadvantage of liners materials is the difficulty of biofilm control. It was compared an experimental dentifrice contained Ricinus communis, with commercials dentifrices as antibiofilm activity against microorganisms on denture liner. Six hundred specimens were distributed in 5 groups (n=18/ microorganism): water; experimental dentifrice; specific dentifrice for denture and two conventional dentifrices against C. albicans; C. glabrata; S. mutans; S. aureus; E. coli. Each group had a negative (n=5; without contamination) and positive control (n=15/ microorganism; without cleaning). The antibiofilm activity was evaluated by the method of biofilm formation in triplicate. The specimens were contaminated in a standard way and incubated. After that, manual brushing was performed (60 s), washed with PBS, immersed in liquid culture medium for resuspension and sowing in solid medium. The results (mean of triplicates) were expressed in CFU/mL. The data was submitted to Shapiro-Wilk, ANOVA and Tukey test (p<0.05). The specific dentifrice (1.27±1.20) was the most effective against S. mutans, followed by conventional (Trihydral, 3.13±0.88; Colgate, 2.16±2.02) and experimental (3.81±1.37) dentifrices, which were similar to each other (p=0.008). All of them were different from water (4.79±1.42). The specific (0.21±0.21) and experimental (0.36±0.25) dentifrices were similar against S. aureus, with a higher mean of CFU when compared to conventional (Colgate, 0.06±0.13), which was more efficient (p=0.000). For C. albicans, C. glabrata and E. coli, all dentifrices were similar to water (p=0.186). It was concluded, that the experimental dentifrice was effective against S. aureus and had not efficacy against Candida spp.; S. mutans; E. coli, as occurred with the commercials dentifrices.
publishDate 2019
dc.date.none.fl_str_mv 2019-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402019000300252
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402019000300252
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0103-6440201902326
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Fundação Odontológica de Ribeirão Preto
publisher.none.fl_str_mv Fundação Odontológica de Ribeirão Preto
dc.source.none.fl_str_mv Brazilian Dental Journal v.30 n.3 2019
reponame:Brazilian Dental Journal
instname:Fundação Odontológica de Ribeirão Preto (FUNORP)
instacron:FUNORP
instname_str Fundação Odontológica de Ribeirão Preto (FUNORP)
instacron_str FUNORP
institution FUNORP
reponame_str Brazilian Dental Journal
collection Brazilian Dental Journal
repository.name.fl_str_mv Brazilian Dental Journal - Fundação Odontológica de Ribeirão Preto (FUNORP)
repository.mail.fl_str_mv bdj@forp.usp.br||sergio@fosjc.unesp.br
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