Genotypic diversity of S. mutans in dental biofilm formed in situ under sugar stress exposure
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Dental Journal |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402007000300002 |
Resumo: | In situ dental biofilm composition under sugar exposure is well known, but sugar effect on the genotypic diversity of S. mutans in dental biofilm has not been explored. This study evaluated S. mutans genotypic diversity in dental biofilm formed in situ under frequent exposure to sucrose and its monosaccharide constituents (glucose and fructose). Saliva of 7 volunteers was collected for isolation of S. mutans and the same volunteers wore intraoral palatal appliances, containing enamel slabs, which were submitted to the following treatments: distilled and deionized water (negative control), 10% glucose + 10% fructose (fermentable carbohydrates) solution or 20% sucrose (fermentable and EPS inductor) solution, 8x/day. After 3, 7 and 14 days, the biofilms were colleted and S. mutans colonies were isolated. Arbitrarily primed polymerase chain reaction (AP-PCR) of S. mutans showed that salivary genotypes were also detected in almost all biofilm samples, independently of the treatment, and seemed to reflect those genotypes present at higher proportion in biofilms. In addition to the salivary genotypes, others were found in biofilms but in lower proportions and were distinct among treatment. The data suggest that the in situ model seems to be useful to evaluate genotypic diversity of S. mutans, but, under the tested conditions, it was not possible to clearly show that specific genotypes were selected in the biofilm due to the stress induced by sucrose metabolism or simple fermentation of its monosaccharides. |
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Brazilian Dental Journal |
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Genotypic diversity of S. mutans in dental biofilm formed in situ under sugar stress exposuresucrosegenotypesS. mutansarbitrarily primed polymerase chain reactionin situ studyIn situ dental biofilm composition under sugar exposure is well known, but sugar effect on the genotypic diversity of S. mutans in dental biofilm has not been explored. This study evaluated S. mutans genotypic diversity in dental biofilm formed in situ under frequent exposure to sucrose and its monosaccharide constituents (glucose and fructose). Saliva of 7 volunteers was collected for isolation of S. mutans and the same volunteers wore intraoral palatal appliances, containing enamel slabs, which were submitted to the following treatments: distilled and deionized water (negative control), 10% glucose + 10% fructose (fermentable carbohydrates) solution or 20% sucrose (fermentable and EPS inductor) solution, 8x/day. After 3, 7 and 14 days, the biofilms were colleted and S. mutans colonies were isolated. Arbitrarily primed polymerase chain reaction (AP-PCR) of S. mutans showed that salivary genotypes were also detected in almost all biofilm samples, independently of the treatment, and seemed to reflect those genotypes present at higher proportion in biofilms. In addition to the salivary genotypes, others were found in biofilms but in lower proportions and were distinct among treatment. The data suggest that the in situ model seems to be useful to evaluate genotypic diversity of S. mutans, but, under the tested conditions, it was not possible to clearly show that specific genotypes were selected in the biofilm due to the stress induced by sucrose metabolism or simple fermentation of its monosaccharides.Fundação Odontológica de Ribeirão Preto2007-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402007000300002Brazilian Dental Journal v.18 n.3 2007reponame:Brazilian Dental Journalinstname:Fundação Odontológica de Ribeirão Preto (FUNORP)instacron:FUNORP10.1590/S0103-64402007000300002info:eu-repo/semantics/openAccessArthur,Rodrigo AlexTabchoury,Cínthia Pereira MachadoMattos-Graner,Renata de OliveiraDel Bel Cury,Altair A.Leme,Adriana Franco PaesVale,Gláuber CamposCury,Jaime Aparecidoeng2008-01-02T00:00:00Zoai:scielo:S0103-64402007000300002Revistahttps://www.scielo.br/j/bdj/https://old.scielo.br/oai/scielo-oai.phpbdj@forp.usp.br||sergio@fosjc.unesp.br1806-47600103-6440opendoar:2008-01-02T00:00Brazilian Dental Journal - Fundação Odontológica de Ribeirão Preto (FUNORP)false |
dc.title.none.fl_str_mv |
Genotypic diversity of S. mutans in dental biofilm formed in situ under sugar stress exposure |
title |
Genotypic diversity of S. mutans in dental biofilm formed in situ under sugar stress exposure |
spellingShingle |
Genotypic diversity of S. mutans in dental biofilm formed in situ under sugar stress exposure Arthur,Rodrigo Alex sucrose genotypes S. mutans arbitrarily primed polymerase chain reaction in situ study |
title_short |
Genotypic diversity of S. mutans in dental biofilm formed in situ under sugar stress exposure |
title_full |
Genotypic diversity of S. mutans in dental biofilm formed in situ under sugar stress exposure |
title_fullStr |
Genotypic diversity of S. mutans in dental biofilm formed in situ under sugar stress exposure |
title_full_unstemmed |
Genotypic diversity of S. mutans in dental biofilm formed in situ under sugar stress exposure |
title_sort |
Genotypic diversity of S. mutans in dental biofilm formed in situ under sugar stress exposure |
author |
Arthur,Rodrigo Alex |
author_facet |
Arthur,Rodrigo Alex Tabchoury,Cínthia Pereira Machado Mattos-Graner,Renata de Oliveira Del Bel Cury,Altair A. Leme,Adriana Franco Paes Vale,Gláuber Campos Cury,Jaime Aparecido |
author_role |
author |
author2 |
Tabchoury,Cínthia Pereira Machado Mattos-Graner,Renata de Oliveira Del Bel Cury,Altair A. Leme,Adriana Franco Paes Vale,Gláuber Campos Cury,Jaime Aparecido |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Arthur,Rodrigo Alex Tabchoury,Cínthia Pereira Machado Mattos-Graner,Renata de Oliveira Del Bel Cury,Altair A. Leme,Adriana Franco Paes Vale,Gláuber Campos Cury,Jaime Aparecido |
dc.subject.por.fl_str_mv |
sucrose genotypes S. mutans arbitrarily primed polymerase chain reaction in situ study |
topic |
sucrose genotypes S. mutans arbitrarily primed polymerase chain reaction in situ study |
description |
In situ dental biofilm composition under sugar exposure is well known, but sugar effect on the genotypic diversity of S. mutans in dental biofilm has not been explored. This study evaluated S. mutans genotypic diversity in dental biofilm formed in situ under frequent exposure to sucrose and its monosaccharide constituents (glucose and fructose). Saliva of 7 volunteers was collected for isolation of S. mutans and the same volunteers wore intraoral palatal appliances, containing enamel slabs, which were submitted to the following treatments: distilled and deionized water (negative control), 10% glucose + 10% fructose (fermentable carbohydrates) solution or 20% sucrose (fermentable and EPS inductor) solution, 8x/day. After 3, 7 and 14 days, the biofilms were colleted and S. mutans colonies were isolated. Arbitrarily primed polymerase chain reaction (AP-PCR) of S. mutans showed that salivary genotypes were also detected in almost all biofilm samples, independently of the treatment, and seemed to reflect those genotypes present at higher proportion in biofilms. In addition to the salivary genotypes, others were found in biofilms but in lower proportions and were distinct among treatment. The data suggest that the in situ model seems to be useful to evaluate genotypic diversity of S. mutans, but, under the tested conditions, it was not possible to clearly show that specific genotypes were selected in the biofilm due to the stress induced by sucrose metabolism or simple fermentation of its monosaccharides. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402007000300002 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402007000300002 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0103-64402007000300002 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Fundação Odontológica de Ribeirão Preto |
publisher.none.fl_str_mv |
Fundação Odontológica de Ribeirão Preto |
dc.source.none.fl_str_mv |
Brazilian Dental Journal v.18 n.3 2007 reponame:Brazilian Dental Journal instname:Fundação Odontológica de Ribeirão Preto (FUNORP) instacron:FUNORP |
instname_str |
Fundação Odontológica de Ribeirão Preto (FUNORP) |
instacron_str |
FUNORP |
institution |
FUNORP |
reponame_str |
Brazilian Dental Journal |
collection |
Brazilian Dental Journal |
repository.name.fl_str_mv |
Brazilian Dental Journal - Fundação Odontológica de Ribeirão Preto (FUNORP) |
repository.mail.fl_str_mv |
bdj@forp.usp.br||sergio@fosjc.unesp.br |
_version_ |
1754204090522402816 |