Chronic Periodontitis and RANKL/OPG Ratio in Peri-Implant Mucosae Inflammation
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Dental Journal |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402018000100014 |
Resumo: | Abstract tHistory of chronic periodontitis (CP) is a risk factor for oseointegration failure. The osteoclastogenesis system (RANK, RANKL and OPG) is critical for bone homeostatic control. We investigated the levels of OPG and RANKL in peri-implant tissues from volunteers with and without a history of CP and their association with mucosae inflammation. This is a single-blind case-contro study. Diagnosis of a history of CP and peri-implant examination was performed on 46 volunteers, divided into control (without history of CP, n=26) and CP group (with history of CP, n=20). Gingival biopsies were harvested during implant exposure. Quantitative PCR evaluated OPG/RANKL mRNA expressions. OPG and RANKL proteins were analyzed by western blot and immunohistochemistry assay. The chi-square test analyzed the significance of nominal variables between groups while continuous variables were analyzed by T-test or Mann-Whitney test, after Shapiro-Wilk test evaluation. The 2-ΔΔCT Livak method calculation evaluated the gene expression. Values of p<0.05 were considered statistically significant. Volunteers with CP history had 23 times higher chance of developing mucosae inflammation. High mucosae levels of RANKL (p=0.04) and RANKL/OPG (p=0.001) mRNA expressions were observed in CP group. CP volunteers showed increased RANKL protein levels in opposition to decreased OPG expression. Even without active periodontitis, volunteers with a history of CP had elevated gingival levels of RANKL/OPG and higher correlation with peri-implant mucosae inflammation and implant loss. |
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Chronic Periodontitis and RANKL/OPG Ratio in Peri-Implant Mucosae InflammationosteoprotegerinRANK ligandchronic periodontitismucositisosseointegrationAbstract tHistory of chronic periodontitis (CP) is a risk factor for oseointegration failure. The osteoclastogenesis system (RANK, RANKL and OPG) is critical for bone homeostatic control. We investigated the levels of OPG and RANKL in peri-implant tissues from volunteers with and without a history of CP and their association with mucosae inflammation. This is a single-blind case-contro study. Diagnosis of a history of CP and peri-implant examination was performed on 46 volunteers, divided into control (without history of CP, n=26) and CP group (with history of CP, n=20). Gingival biopsies were harvested during implant exposure. Quantitative PCR evaluated OPG/RANKL mRNA expressions. OPG and RANKL proteins were analyzed by western blot and immunohistochemistry assay. The chi-square test analyzed the significance of nominal variables between groups while continuous variables were analyzed by T-test or Mann-Whitney test, after Shapiro-Wilk test evaluation. The 2-ΔΔCT Livak method calculation evaluated the gene expression. Values of p<0.05 were considered statistically significant. Volunteers with CP history had 23 times higher chance of developing mucosae inflammation. High mucosae levels of RANKL (p=0.04) and RANKL/OPG (p=0.001) mRNA expressions were observed in CP group. CP volunteers showed increased RANKL protein levels in opposition to decreased OPG expression. Even without active periodontitis, volunteers with a history of CP had elevated gingival levels of RANKL/OPG and higher correlation with peri-implant mucosae inflammation and implant loss.Fundação Odontológica de Ribeirão Preto2018-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402018000100014Brazilian Dental Journal v.29 n.1 2018reponame:Brazilian Dental Journalinstname:Fundação Odontológica de Ribeirão Preto (FUNORP)instacron:FUNORP10.1590/0103-6440201801241info:eu-repo/semantics/openAccessCosta,Lucas CarneiroFonseca,Marcos Alexandre daPinheiro,Aristides da RosaAguiar,Telma Regina da SilvaMachado,Aldir NascimentoQuinelato,ValquiriaBonato,Leticia LadeiraAguiar,Diego PinheiroVieira,ThaysAlmeida,Fernando Luiz Duarte deLobo,Julie CalixtoJordão,MiriamLomardo,Priscilla GonçalvesGranjeiro,José MauroCasado,Priscila Ladeiraeng2018-03-14T00:00:00Zoai:scielo:S0103-64402018000100014Revistahttps://www.scielo.br/j/bdj/https://old.scielo.br/oai/scielo-oai.phpbdj@forp.usp.br||sergio@fosjc.unesp.br1806-47600103-6440opendoar:2018-03-14T00:00Brazilian Dental Journal - Fundação Odontológica de Ribeirão Preto (FUNORP)false |
dc.title.none.fl_str_mv |
Chronic Periodontitis and RANKL/OPG Ratio in Peri-Implant Mucosae Inflammation |
title |
Chronic Periodontitis and RANKL/OPG Ratio in Peri-Implant Mucosae Inflammation |
spellingShingle |
Chronic Periodontitis and RANKL/OPG Ratio in Peri-Implant Mucosae Inflammation Costa,Lucas Carneiro osteoprotegerin RANK ligand chronic periodontitis mucositis osseointegration |
title_short |
Chronic Periodontitis and RANKL/OPG Ratio in Peri-Implant Mucosae Inflammation |
title_full |
Chronic Periodontitis and RANKL/OPG Ratio in Peri-Implant Mucosae Inflammation |
title_fullStr |
Chronic Periodontitis and RANKL/OPG Ratio in Peri-Implant Mucosae Inflammation |
title_full_unstemmed |
Chronic Periodontitis and RANKL/OPG Ratio in Peri-Implant Mucosae Inflammation |
title_sort |
Chronic Periodontitis and RANKL/OPG Ratio in Peri-Implant Mucosae Inflammation |
author |
Costa,Lucas Carneiro |
author_facet |
Costa,Lucas Carneiro Fonseca,Marcos Alexandre da Pinheiro,Aristides da Rosa Aguiar,Telma Regina da Silva Machado,Aldir Nascimento Quinelato,Valquiria Bonato,Leticia Ladeira Aguiar,Diego Pinheiro Vieira,Thays Almeida,Fernando Luiz Duarte de Lobo,Julie Calixto Jordão,Miriam Lomardo,Priscilla Gonçalves Granjeiro,José Mauro Casado,Priscila Ladeira |
author_role |
author |
author2 |
Fonseca,Marcos Alexandre da Pinheiro,Aristides da Rosa Aguiar,Telma Regina da Silva Machado,Aldir Nascimento Quinelato,Valquiria Bonato,Leticia Ladeira Aguiar,Diego Pinheiro Vieira,Thays Almeida,Fernando Luiz Duarte de Lobo,Julie Calixto Jordão,Miriam Lomardo,Priscilla Gonçalves Granjeiro,José Mauro Casado,Priscila Ladeira |
author2_role |
author author author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Costa,Lucas Carneiro Fonseca,Marcos Alexandre da Pinheiro,Aristides da Rosa Aguiar,Telma Regina da Silva Machado,Aldir Nascimento Quinelato,Valquiria Bonato,Leticia Ladeira Aguiar,Diego Pinheiro Vieira,Thays Almeida,Fernando Luiz Duarte de Lobo,Julie Calixto Jordão,Miriam Lomardo,Priscilla Gonçalves Granjeiro,José Mauro Casado,Priscila Ladeira |
dc.subject.por.fl_str_mv |
osteoprotegerin RANK ligand chronic periodontitis mucositis osseointegration |
topic |
osteoprotegerin RANK ligand chronic periodontitis mucositis osseointegration |
description |
Abstract tHistory of chronic periodontitis (CP) is a risk factor for oseointegration failure. The osteoclastogenesis system (RANK, RANKL and OPG) is critical for bone homeostatic control. We investigated the levels of OPG and RANKL in peri-implant tissues from volunteers with and without a history of CP and their association with mucosae inflammation. This is a single-blind case-contro study. Diagnosis of a history of CP and peri-implant examination was performed on 46 volunteers, divided into control (without history of CP, n=26) and CP group (with history of CP, n=20). Gingival biopsies were harvested during implant exposure. Quantitative PCR evaluated OPG/RANKL mRNA expressions. OPG and RANKL proteins were analyzed by western blot and immunohistochemistry assay. The chi-square test analyzed the significance of nominal variables between groups while continuous variables were analyzed by T-test or Mann-Whitney test, after Shapiro-Wilk test evaluation. The 2-ΔΔCT Livak method calculation evaluated the gene expression. Values of p<0.05 were considered statistically significant. Volunteers with CP history had 23 times higher chance of developing mucosae inflammation. High mucosae levels of RANKL (p=0.04) and RANKL/OPG (p=0.001) mRNA expressions were observed in CP group. CP volunteers showed increased RANKL protein levels in opposition to decreased OPG expression. Even without active periodontitis, volunteers with a history of CP had elevated gingival levels of RANKL/OPG and higher correlation with peri-implant mucosae inflammation and implant loss. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-02-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402018000100014 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402018000100014 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0103-6440201801241 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Fundação Odontológica de Ribeirão Preto |
publisher.none.fl_str_mv |
Fundação Odontológica de Ribeirão Preto |
dc.source.none.fl_str_mv |
Brazilian Dental Journal v.29 n.1 2018 reponame:Brazilian Dental Journal instname:Fundação Odontológica de Ribeirão Preto (FUNORP) instacron:FUNORP |
instname_str |
Fundação Odontológica de Ribeirão Preto (FUNORP) |
instacron_str |
FUNORP |
institution |
FUNORP |
reponame_str |
Brazilian Dental Journal |
collection |
Brazilian Dental Journal |
repository.name.fl_str_mv |
Brazilian Dental Journal - Fundação Odontológica de Ribeirão Preto (FUNORP) |
repository.mail.fl_str_mv |
bdj@forp.usp.br||sergio@fosjc.unesp.br |
_version_ |
1754204094909644800 |