Chronic Periodontitis and RANKL/OPG Ratio in Peri-Implant Mucosae Inflammation

Detalhes bibliográficos
Autor(a) principal: Costa,Lucas Carneiro
Data de Publicação: 2018
Outros Autores: Fonseca,Marcos Alexandre da, Pinheiro,Aristides da Rosa, Aguiar,Telma Regina da Silva, Machado,Aldir Nascimento, Quinelato,Valquiria, Bonato,Leticia Ladeira, Aguiar,Diego Pinheiro, Vieira,Thays, Almeida,Fernando Luiz Duarte de, Lobo,Julie Calixto, Jordão,Miriam, Lomardo,Priscilla Gonçalves, Granjeiro,José Mauro, Casado,Priscila Ladeira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Dental Journal
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402018000100014
Resumo: Abstract tHistory of chronic periodontitis (CP) is a risk factor for oseointegration failure. The osteoclastogenesis system (RANK, RANKL and OPG) is critical for bone homeostatic control. We investigated the levels of OPG and RANKL in peri-implant tissues from volunteers with and without a history of CP and their association with mucosae inflammation. This is a single-blind case-contro study. Diagnosis of a history of CP and peri-implant examination was performed on 46 volunteers, divided into control (without history of CP, n=26) and CP group (with history of CP, n=20). Gingival biopsies were harvested during implant exposure. Quantitative PCR evaluated OPG/RANKL mRNA expressions. OPG and RANKL proteins were analyzed by western blot and immunohistochemistry assay. The chi-square test analyzed the significance of nominal variables between groups while continuous variables were analyzed by T-test or Mann-Whitney test, after Shapiro-Wilk test evaluation. The 2-ΔΔCT Livak method calculation evaluated the gene expression. Values of p<0.05 were considered statistically significant. Volunteers with CP history had 23 times higher chance of developing mucosae inflammation. High mucosae levels of RANKL (p=0.04) and RANKL/OPG (p=0.001) mRNA expressions were observed in CP group. CP volunteers showed increased RANKL protein levels in opposition to decreased OPG expression. Even without active periodontitis, volunteers with a history of CP had elevated gingival levels of RANKL/OPG and higher correlation with peri-implant mucosae inflammation and implant loss.
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spelling Chronic Periodontitis and RANKL/OPG Ratio in Peri-Implant Mucosae InflammationosteoprotegerinRANK ligandchronic periodontitismucositisosseointegrationAbstract tHistory of chronic periodontitis (CP) is a risk factor for oseointegration failure. The osteoclastogenesis system (RANK, RANKL and OPG) is critical for bone homeostatic control. We investigated the levels of OPG and RANKL in peri-implant tissues from volunteers with and without a history of CP and their association with mucosae inflammation. This is a single-blind case-contro study. Diagnosis of a history of CP and peri-implant examination was performed on 46 volunteers, divided into control (without history of CP, n=26) and CP group (with history of CP, n=20). Gingival biopsies were harvested during implant exposure. Quantitative PCR evaluated OPG/RANKL mRNA expressions. OPG and RANKL proteins were analyzed by western blot and immunohistochemistry assay. The chi-square test analyzed the significance of nominal variables between groups while continuous variables were analyzed by T-test or Mann-Whitney test, after Shapiro-Wilk test evaluation. The 2-ΔΔCT Livak method calculation evaluated the gene expression. Values of p<0.05 were considered statistically significant. Volunteers with CP history had 23 times higher chance of developing mucosae inflammation. High mucosae levels of RANKL (p=0.04) and RANKL/OPG (p=0.001) mRNA expressions were observed in CP group. CP volunteers showed increased RANKL protein levels in opposition to decreased OPG expression. Even without active periodontitis, volunteers with a history of CP had elevated gingival levels of RANKL/OPG and higher correlation with peri-implant mucosae inflammation and implant loss.Fundação Odontológica de Ribeirão Preto2018-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402018000100014Brazilian Dental Journal v.29 n.1 2018reponame:Brazilian Dental Journalinstname:Fundação Odontológica de Ribeirão Preto (FUNORP)instacron:FUNORP10.1590/0103-6440201801241info:eu-repo/semantics/openAccessCosta,Lucas CarneiroFonseca,Marcos Alexandre daPinheiro,Aristides da RosaAguiar,Telma Regina da SilvaMachado,Aldir NascimentoQuinelato,ValquiriaBonato,Leticia LadeiraAguiar,Diego PinheiroVieira,ThaysAlmeida,Fernando Luiz Duarte deLobo,Julie CalixtoJordão,MiriamLomardo,Priscilla GonçalvesGranjeiro,José MauroCasado,Priscila Ladeiraeng2018-03-14T00:00:00Zoai:scielo:S0103-64402018000100014Revistahttps://www.scielo.br/j/bdj/https://old.scielo.br/oai/scielo-oai.phpbdj@forp.usp.br||sergio@fosjc.unesp.br1806-47600103-6440opendoar:2018-03-14T00:00Brazilian Dental Journal - Fundação Odontológica de Ribeirão Preto (FUNORP)false
dc.title.none.fl_str_mv Chronic Periodontitis and RANKL/OPG Ratio in Peri-Implant Mucosae Inflammation
title Chronic Periodontitis and RANKL/OPG Ratio in Peri-Implant Mucosae Inflammation
spellingShingle Chronic Periodontitis and RANKL/OPG Ratio in Peri-Implant Mucosae Inflammation
Costa,Lucas Carneiro
osteoprotegerin
RANK ligand
chronic periodontitis
mucositis
osseointegration
title_short Chronic Periodontitis and RANKL/OPG Ratio in Peri-Implant Mucosae Inflammation
title_full Chronic Periodontitis and RANKL/OPG Ratio in Peri-Implant Mucosae Inflammation
title_fullStr Chronic Periodontitis and RANKL/OPG Ratio in Peri-Implant Mucosae Inflammation
title_full_unstemmed Chronic Periodontitis and RANKL/OPG Ratio in Peri-Implant Mucosae Inflammation
title_sort Chronic Periodontitis and RANKL/OPG Ratio in Peri-Implant Mucosae Inflammation
author Costa,Lucas Carneiro
author_facet Costa,Lucas Carneiro
Fonseca,Marcos Alexandre da
Pinheiro,Aristides da Rosa
Aguiar,Telma Regina da Silva
Machado,Aldir Nascimento
Quinelato,Valquiria
Bonato,Leticia Ladeira
Aguiar,Diego Pinheiro
Vieira,Thays
Almeida,Fernando Luiz Duarte de
Lobo,Julie Calixto
Jordão,Miriam
Lomardo,Priscilla Gonçalves
Granjeiro,José Mauro
Casado,Priscila Ladeira
author_role author
author2 Fonseca,Marcos Alexandre da
Pinheiro,Aristides da Rosa
Aguiar,Telma Regina da Silva
Machado,Aldir Nascimento
Quinelato,Valquiria
Bonato,Leticia Ladeira
Aguiar,Diego Pinheiro
Vieira,Thays
Almeida,Fernando Luiz Duarte de
Lobo,Julie Calixto
Jordão,Miriam
Lomardo,Priscilla Gonçalves
Granjeiro,José Mauro
Casado,Priscila Ladeira
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Costa,Lucas Carneiro
Fonseca,Marcos Alexandre da
Pinheiro,Aristides da Rosa
Aguiar,Telma Regina da Silva
Machado,Aldir Nascimento
Quinelato,Valquiria
Bonato,Leticia Ladeira
Aguiar,Diego Pinheiro
Vieira,Thays
Almeida,Fernando Luiz Duarte de
Lobo,Julie Calixto
Jordão,Miriam
Lomardo,Priscilla Gonçalves
Granjeiro,José Mauro
Casado,Priscila Ladeira
dc.subject.por.fl_str_mv osteoprotegerin
RANK ligand
chronic periodontitis
mucositis
osseointegration
topic osteoprotegerin
RANK ligand
chronic periodontitis
mucositis
osseointegration
description Abstract tHistory of chronic periodontitis (CP) is a risk factor for oseointegration failure. The osteoclastogenesis system (RANK, RANKL and OPG) is critical for bone homeostatic control. We investigated the levels of OPG and RANKL in peri-implant tissues from volunteers with and without a history of CP and their association with mucosae inflammation. This is a single-blind case-contro study. Diagnosis of a history of CP and peri-implant examination was performed on 46 volunteers, divided into control (without history of CP, n=26) and CP group (with history of CP, n=20). Gingival biopsies were harvested during implant exposure. Quantitative PCR evaluated OPG/RANKL mRNA expressions. OPG and RANKL proteins were analyzed by western blot and immunohistochemistry assay. The chi-square test analyzed the significance of nominal variables between groups while continuous variables were analyzed by T-test or Mann-Whitney test, after Shapiro-Wilk test evaluation. The 2-ΔΔCT Livak method calculation evaluated the gene expression. Values of p<0.05 were considered statistically significant. Volunteers with CP history had 23 times higher chance of developing mucosae inflammation. High mucosae levels of RANKL (p=0.04) and RANKL/OPG (p=0.001) mRNA expressions were observed in CP group. CP volunteers showed increased RANKL protein levels in opposition to decreased OPG expression. Even without active periodontitis, volunteers with a history of CP had elevated gingival levels of RANKL/OPG and higher correlation with peri-implant mucosae inflammation and implant loss.
publishDate 2018
dc.date.none.fl_str_mv 2018-02-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402018000100014
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402018000100014
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0103-6440201801241
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Fundação Odontológica de Ribeirão Preto
publisher.none.fl_str_mv Fundação Odontológica de Ribeirão Preto
dc.source.none.fl_str_mv Brazilian Dental Journal v.29 n.1 2018
reponame:Brazilian Dental Journal
instname:Fundação Odontológica de Ribeirão Preto (FUNORP)
instacron:FUNORP
instname_str Fundação Odontológica de Ribeirão Preto (FUNORP)
instacron_str FUNORP
institution FUNORP
reponame_str Brazilian Dental Journal
collection Brazilian Dental Journal
repository.name.fl_str_mv Brazilian Dental Journal - Fundação Odontológica de Ribeirão Preto (FUNORP)
repository.mail.fl_str_mv bdj@forp.usp.br||sergio@fosjc.unesp.br
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