Effects of a 10% carbamide peroxide bleaching agent on rat oral epithelium proliferation

Detalhes bibliográficos
Autor(a) principal: Albuquerque,Rodrigo de Castro
Data de Publicação: 2002
Outros Autores: Gomez,Ricardo Santiago, Dutra,Rodrigo Aliprandi, Vasconcellos,Wallison Arthuso, Gomez,Renato Santiago, Gomez,Marcus Vinícius
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Dental Journal
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402002000300003
Resumo: The purpose of the present study was to evaluate the influence of short course topical application of carbamide peroxide on proliferating cell nuclear antigen (PCNA) immunohistochemical expression in the oral tongue mucosa of rats. Twelve male Wistar rats were submitted to topical application of 10% carbamide peroxide on one side of the dorsal tongue once a week for three consecutive weeks. Only distilled water was applied on the control side. The animals were killed on days 0, 10, and 20 after the last application. The tongue was fixed in buffered formalin for 24 h and embedded in paraffin. Tissue blocks (3 µm) were subjected to the biotin-streptavidin amplified system for identification of PCNA. The percentage of epithelial-positive basal cells in each side of the tongue mucosa was calculated. The results demonstrated that topical application of 10% carbamide peroxide increases PCNA immunohistochemical expression on the basal layer of the oral mucosa epithelium of rats on day 0 after treatment. In conclusion, short-course use of carbamide peroxide induces transient epithelial cell proliferation of the oral mucosa of rats.
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spelling Effects of a 10% carbamide peroxide bleaching agent on rat oral epithelium proliferationcarbamide peroxideimmunocytochemistryoral mucosaproliferating cell nuclear antigencarcinogenesisThe purpose of the present study was to evaluate the influence of short course topical application of carbamide peroxide on proliferating cell nuclear antigen (PCNA) immunohistochemical expression in the oral tongue mucosa of rats. Twelve male Wistar rats were submitted to topical application of 10% carbamide peroxide on one side of the dorsal tongue once a week for three consecutive weeks. Only distilled water was applied on the control side. The animals were killed on days 0, 10, and 20 after the last application. The tongue was fixed in buffered formalin for 24 h and embedded in paraffin. Tissue blocks (3 µm) were subjected to the biotin-streptavidin amplified system for identification of PCNA. The percentage of epithelial-positive basal cells in each side of the tongue mucosa was calculated. The results demonstrated that topical application of 10% carbamide peroxide increases PCNA immunohistochemical expression on the basal layer of the oral mucosa epithelium of rats on day 0 after treatment. In conclusion, short-course use of carbamide peroxide induces transient epithelial cell proliferation of the oral mucosa of rats.Fundação Odontológica de Ribeirão Preto2002-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402002000300003Brazilian Dental Journal v.13 n.3 2002reponame:Brazilian Dental Journalinstname:Fundação Odontológica de Ribeirão Preto (FUNORP)instacron:FUNORP10.1590/S0103-64402002000300003info:eu-repo/semantics/openAccessAlbuquerque,Rodrigo de CastroGomez,Ricardo SantiagoDutra,Rodrigo AliprandiVasconcellos,Wallison ArthusoGomez,Renato SantiagoGomez,Marcus Viníciuseng2003-04-07T00:00:00Zoai:scielo:S0103-64402002000300003Revistahttps://www.scielo.br/j/bdj/https://old.scielo.br/oai/scielo-oai.phpbdj@forp.usp.br||sergio@fosjc.unesp.br1806-47600103-6440opendoar:2003-04-07T00:00Brazilian Dental Journal - Fundação Odontológica de Ribeirão Preto (FUNORP)false
dc.title.none.fl_str_mv Effects of a 10% carbamide peroxide bleaching agent on rat oral epithelium proliferation
title Effects of a 10% carbamide peroxide bleaching agent on rat oral epithelium proliferation
spellingShingle Effects of a 10% carbamide peroxide bleaching agent on rat oral epithelium proliferation
Albuquerque,Rodrigo de Castro
carbamide peroxide
immunocytochemistry
oral mucosa
proliferating cell nuclear antigen
carcinogenesis
title_short Effects of a 10% carbamide peroxide bleaching agent on rat oral epithelium proliferation
title_full Effects of a 10% carbamide peroxide bleaching agent on rat oral epithelium proliferation
title_fullStr Effects of a 10% carbamide peroxide bleaching agent on rat oral epithelium proliferation
title_full_unstemmed Effects of a 10% carbamide peroxide bleaching agent on rat oral epithelium proliferation
title_sort Effects of a 10% carbamide peroxide bleaching agent on rat oral epithelium proliferation
author Albuquerque,Rodrigo de Castro
author_facet Albuquerque,Rodrigo de Castro
Gomez,Ricardo Santiago
Dutra,Rodrigo Aliprandi
Vasconcellos,Wallison Arthuso
Gomez,Renato Santiago
Gomez,Marcus Vinícius
author_role author
author2 Gomez,Ricardo Santiago
Dutra,Rodrigo Aliprandi
Vasconcellos,Wallison Arthuso
Gomez,Renato Santiago
Gomez,Marcus Vinícius
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Albuquerque,Rodrigo de Castro
Gomez,Ricardo Santiago
Dutra,Rodrigo Aliprandi
Vasconcellos,Wallison Arthuso
Gomez,Renato Santiago
Gomez,Marcus Vinícius
dc.subject.por.fl_str_mv carbamide peroxide
immunocytochemistry
oral mucosa
proliferating cell nuclear antigen
carcinogenesis
topic carbamide peroxide
immunocytochemistry
oral mucosa
proliferating cell nuclear antigen
carcinogenesis
description The purpose of the present study was to evaluate the influence of short course topical application of carbamide peroxide on proliferating cell nuclear antigen (PCNA) immunohistochemical expression in the oral tongue mucosa of rats. Twelve male Wistar rats were submitted to topical application of 10% carbamide peroxide on one side of the dorsal tongue once a week for three consecutive weeks. Only distilled water was applied on the control side. The animals were killed on days 0, 10, and 20 after the last application. The tongue was fixed in buffered formalin for 24 h and embedded in paraffin. Tissue blocks (3 µm) were subjected to the biotin-streptavidin amplified system for identification of PCNA. The percentage of epithelial-positive basal cells in each side of the tongue mucosa was calculated. The results demonstrated that topical application of 10% carbamide peroxide increases PCNA immunohistochemical expression on the basal layer of the oral mucosa epithelium of rats on day 0 after treatment. In conclusion, short-course use of carbamide peroxide induces transient epithelial cell proliferation of the oral mucosa of rats.
publishDate 2002
dc.date.none.fl_str_mv 2002-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402002000300003
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402002000300003
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0103-64402002000300003
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Fundação Odontológica de Ribeirão Preto
publisher.none.fl_str_mv Fundação Odontológica de Ribeirão Preto
dc.source.none.fl_str_mv Brazilian Dental Journal v.13 n.3 2002
reponame:Brazilian Dental Journal
instname:Fundação Odontológica de Ribeirão Preto (FUNORP)
instacron:FUNORP
instname_str Fundação Odontológica de Ribeirão Preto (FUNORP)
instacron_str FUNORP
institution FUNORP
reponame_str Brazilian Dental Journal
collection Brazilian Dental Journal
repository.name.fl_str_mv Brazilian Dental Journal - Fundação Odontológica de Ribeirão Preto (FUNORP)
repository.mail.fl_str_mv bdj@forp.usp.br||sergio@fosjc.unesp.br
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