Development of Intracanal Formulation Containing Silver Nanoparticles
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Dental Journal |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402014000400302 |
Resumo: | This study aimed to synthetize, characterize and evaluate the antimicrobial properties of silver nanoparticles to be used in the development of a root intracanal formulation. Silver nanoparticles (AgNPs) were obtained by reduction of silver nitrate with sodium borohydride and characterized by UV-Visible spectrophotometry, scanning electron microscopy (SEM) and dynamic light scattering (DLS). The antimicrobial activity of nanoparticle formulation was evaluated by determinations of the minimum inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) against different bacterial species by the microdilution method, according to recommendations of the Clinical and Laboratory Standards Institute (CLSI). Three potential vehicles, hydroxyethylcellulose, Carbomer and polyethylene glycol were tested as carriers for formulations containing AgNPs. The efficiency of the synthesis method chosen to produce AgNPs was demonstrated by four characterization techniques. The nanoparticles showed antibacterial activity against all species tested. Incorporation of AgNPs into all experimental vehicles produced stable formulations but the one in hydroxyethylcellulose presented better physical proprieties. The results indicate that silver nanoparticles are potential antiseptic agents to be used in root canals and incorporation in adequate vehicles may favor a broader application. |
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Brazilian Dental Journal |
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Development of Intracanal Formulation Containing Silver NanoparticlesEndodonticsdental pulp diseasesnanoparticlessilverantibacterial agentsThis study aimed to synthetize, characterize and evaluate the antimicrobial properties of silver nanoparticles to be used in the development of a root intracanal formulation. Silver nanoparticles (AgNPs) were obtained by reduction of silver nitrate with sodium borohydride and characterized by UV-Visible spectrophotometry, scanning electron microscopy (SEM) and dynamic light scattering (DLS). The antimicrobial activity of nanoparticle formulation was evaluated by determinations of the minimum inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) against different bacterial species by the microdilution method, according to recommendations of the Clinical and Laboratory Standards Institute (CLSI). Three potential vehicles, hydroxyethylcellulose, Carbomer and polyethylene glycol were tested as carriers for formulations containing AgNPs. The efficiency of the synthesis method chosen to produce AgNPs was demonstrated by four characterization techniques. The nanoparticles showed antibacterial activity against all species tested. Incorporation of AgNPs into all experimental vehicles produced stable formulations but the one in hydroxyethylcellulose presented better physical proprieties. The results indicate that silver nanoparticles are potential antiseptic agents to be used in root canals and incorporation in adequate vehicles may favor a broader application.Fundação Odontológica de Ribeirão Preto2014-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402014000400302Brazilian Dental Journal v.25 n.4 2014reponame:Brazilian Dental Journalinstname:Fundação Odontológica de Ribeirão Preto (FUNORP)instacron:FUNORP10.1590/0103-6440201302431info:eu-repo/semantics/openAccessBruniera,João Felipe BonattoSilva-Sousa,Yara Teresinha CorrêaLara,Marilisa GuimarãesPitondo-Silva,AndréMarcaccini,Andrea MarciaMiranda,Carlos Eduardo Saraivaeng2014-09-18T00:00:00Zoai:scielo:S0103-64402014000400302Revistahttps://www.scielo.br/j/bdj/https://old.scielo.br/oai/scielo-oai.phpbdj@forp.usp.br||sergio@fosjc.unesp.br1806-47600103-6440opendoar:2014-09-18T00:00Brazilian Dental Journal - Fundação Odontológica de Ribeirão Preto (FUNORP)false |
dc.title.none.fl_str_mv |
Development of Intracanal Formulation Containing Silver Nanoparticles |
title |
Development of Intracanal Formulation Containing Silver Nanoparticles |
spellingShingle |
Development of Intracanal Formulation Containing Silver Nanoparticles Bruniera,João Felipe Bonatto Endodontics dental pulp diseases nanoparticles silver antibacterial agents |
title_short |
Development of Intracanal Formulation Containing Silver Nanoparticles |
title_full |
Development of Intracanal Formulation Containing Silver Nanoparticles |
title_fullStr |
Development of Intracanal Formulation Containing Silver Nanoparticles |
title_full_unstemmed |
Development of Intracanal Formulation Containing Silver Nanoparticles |
title_sort |
Development of Intracanal Formulation Containing Silver Nanoparticles |
author |
Bruniera,João Felipe Bonatto |
author_facet |
Bruniera,João Felipe Bonatto Silva-Sousa,Yara Teresinha Corrêa Lara,Marilisa Guimarães Pitondo-Silva,André Marcaccini,Andrea Marcia Miranda,Carlos Eduardo Saraiva |
author_role |
author |
author2 |
Silva-Sousa,Yara Teresinha Corrêa Lara,Marilisa Guimarães Pitondo-Silva,André Marcaccini,Andrea Marcia Miranda,Carlos Eduardo Saraiva |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Bruniera,João Felipe Bonatto Silva-Sousa,Yara Teresinha Corrêa Lara,Marilisa Guimarães Pitondo-Silva,André Marcaccini,Andrea Marcia Miranda,Carlos Eduardo Saraiva |
dc.subject.por.fl_str_mv |
Endodontics dental pulp diseases nanoparticles silver antibacterial agents |
topic |
Endodontics dental pulp diseases nanoparticles silver antibacterial agents |
description |
This study aimed to synthetize, characterize and evaluate the antimicrobial properties of silver nanoparticles to be used in the development of a root intracanal formulation. Silver nanoparticles (AgNPs) were obtained by reduction of silver nitrate with sodium borohydride and characterized by UV-Visible spectrophotometry, scanning electron microscopy (SEM) and dynamic light scattering (DLS). The antimicrobial activity of nanoparticle formulation was evaluated by determinations of the minimum inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) against different bacterial species by the microdilution method, according to recommendations of the Clinical and Laboratory Standards Institute (CLSI). Three potential vehicles, hydroxyethylcellulose, Carbomer and polyethylene glycol were tested as carriers for formulations containing AgNPs. The efficiency of the synthesis method chosen to produce AgNPs was demonstrated by four characterization techniques. The nanoparticles showed antibacterial activity against all species tested. Incorporation of AgNPs into all experimental vehicles produced stable formulations but the one in hydroxyethylcellulose presented better physical proprieties. The results indicate that silver nanoparticles are potential antiseptic agents to be used in root canals and incorporation in adequate vehicles may favor a broader application. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402014000400302 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402014000400302 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0103-6440201302431 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Fundação Odontológica de Ribeirão Preto |
publisher.none.fl_str_mv |
Fundação Odontológica de Ribeirão Preto |
dc.source.none.fl_str_mv |
Brazilian Dental Journal v.25 n.4 2014 reponame:Brazilian Dental Journal instname:Fundação Odontológica de Ribeirão Preto (FUNORP) instacron:FUNORP |
instname_str |
Fundação Odontológica de Ribeirão Preto (FUNORP) |
instacron_str |
FUNORP |
institution |
FUNORP |
reponame_str |
Brazilian Dental Journal |
collection |
Brazilian Dental Journal |
repository.name.fl_str_mv |
Brazilian Dental Journal - Fundação Odontológica de Ribeirão Preto (FUNORP) |
repository.mail.fl_str_mv |
bdj@forp.usp.br||sergio@fosjc.unesp.br |
_version_ |
1754204093116579840 |