Impact of smoking on protein levels of beta-defensins in periodontal disease

Detalhes bibliográficos
Autor(a) principal: Soldati,Kahena Rodrigues
Data de Publicação: 2022
Outros Autores: Gutierrez,Lorena Silva, Anovazzi,Giovana, Scarel-Caminaga,Raquel Mantuaneli, Zandim-Barcelos,Daniela Leal
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Dental Journal
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402022000400079
Resumo: Abstract Antimicrobial peptides (AMPs) are important components of the host response against invading pathogens. In addition to their direct antimicrobial activity, they can also participate in the immune system modulation. However, the role of AMPs in the etiopathogenesis of periodontal disease and the risk factors that may influence their expression in the oral cavity are not fully understood. The aim of this study was to determine the impact of smoking on beta-defensin (hBD) 1 and 2 levels analyzing samples from periodontitis patients. Fifty patients with periodontitis, 25 smokers and 25 non-smokers, and 20 periodontally healthy patients were recruited. After periodontal clinical evaluation, gingival crevicular fluid (GCF) samples were collected from healthy sites of patients without periodontal disease and from healthy and diseased sites of patients with periodontitis. Peptides quantification was performed by sandwich ELISA technique. Smokers showed reduced GCF hBD 1 levels and increased hBD 2 levels compared to non-smokers in diseased sites (p <0.05). Higher levels of hBD 1 were observed in healthy sites of patients without periodontal disease than in healthy sites of patients with periodontitis (p<0.0001). Diseased sites of non-smokers presented higher levels of hBD 2 than healthy sites (p <0.05). These results reveal that protein levels of hBDs 1 and 2 can be impaired by cigarette smoking in the presence of periodontal disease.
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spelling Impact of smoking on protein levels of beta-defensins in periodontal diseasebeta-defensinsperiodontitissmokingAbstract Antimicrobial peptides (AMPs) are important components of the host response against invading pathogens. In addition to their direct antimicrobial activity, they can also participate in the immune system modulation. However, the role of AMPs in the etiopathogenesis of periodontal disease and the risk factors that may influence their expression in the oral cavity are not fully understood. The aim of this study was to determine the impact of smoking on beta-defensin (hBD) 1 and 2 levels analyzing samples from periodontitis patients. Fifty patients with periodontitis, 25 smokers and 25 non-smokers, and 20 periodontally healthy patients were recruited. After periodontal clinical evaluation, gingival crevicular fluid (GCF) samples were collected from healthy sites of patients without periodontal disease and from healthy and diseased sites of patients with periodontitis. Peptides quantification was performed by sandwich ELISA technique. Smokers showed reduced GCF hBD 1 levels and increased hBD 2 levels compared to non-smokers in diseased sites (p <0.05). Higher levels of hBD 1 were observed in healthy sites of patients without periodontal disease than in healthy sites of patients with periodontitis (p<0.0001). Diseased sites of non-smokers presented higher levels of hBD 2 than healthy sites (p <0.05). These results reveal that protein levels of hBDs 1 and 2 can be impaired by cigarette smoking in the presence of periodontal disease.Fundação Odontológica de Ribeirão Preto2022-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402022000400079Brazilian Dental Journal v.33 n.4 2022reponame:Brazilian Dental Journalinstname:Fundação Odontológica de Ribeirão Preto (FUNORP)instacron:FUNORP10.1590/0103-6440202204685info:eu-repo/semantics/openAccessSoldati,Kahena RodriguesGutierrez,Lorena SilvaAnovazzi,GiovanaScarel-Caminaga,Raquel MantuaneliZandim-Barcelos,Daniela Lealeng2022-08-23T00:00:00Zoai:scielo:S0103-64402022000400079Revistahttps://www.scielo.br/j/bdj/https://old.scielo.br/oai/scielo-oai.phpbdj@forp.usp.br||sergio@fosjc.unesp.br1806-47600103-6440opendoar:2022-08-23T00:00Brazilian Dental Journal - Fundação Odontológica de Ribeirão Preto (FUNORP)false
dc.title.none.fl_str_mv Impact of smoking on protein levels of beta-defensins in periodontal disease
title Impact of smoking on protein levels of beta-defensins in periodontal disease
spellingShingle Impact of smoking on protein levels of beta-defensins in periodontal disease
Soldati,Kahena Rodrigues
beta-defensins
periodontitis
smoking
title_short Impact of smoking on protein levels of beta-defensins in periodontal disease
title_full Impact of smoking on protein levels of beta-defensins in periodontal disease
title_fullStr Impact of smoking on protein levels of beta-defensins in periodontal disease
title_full_unstemmed Impact of smoking on protein levels of beta-defensins in periodontal disease
title_sort Impact of smoking on protein levels of beta-defensins in periodontal disease
author Soldati,Kahena Rodrigues
author_facet Soldati,Kahena Rodrigues
Gutierrez,Lorena Silva
Anovazzi,Giovana
Scarel-Caminaga,Raquel Mantuaneli
Zandim-Barcelos,Daniela Leal
author_role author
author2 Gutierrez,Lorena Silva
Anovazzi,Giovana
Scarel-Caminaga,Raquel Mantuaneli
Zandim-Barcelos,Daniela Leal
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Soldati,Kahena Rodrigues
Gutierrez,Lorena Silva
Anovazzi,Giovana
Scarel-Caminaga,Raquel Mantuaneli
Zandim-Barcelos,Daniela Leal
dc.subject.por.fl_str_mv beta-defensins
periodontitis
smoking
topic beta-defensins
periodontitis
smoking
description Abstract Antimicrobial peptides (AMPs) are important components of the host response against invading pathogens. In addition to their direct antimicrobial activity, they can also participate in the immune system modulation. However, the role of AMPs in the etiopathogenesis of periodontal disease and the risk factors that may influence their expression in the oral cavity are not fully understood. The aim of this study was to determine the impact of smoking on beta-defensin (hBD) 1 and 2 levels analyzing samples from periodontitis patients. Fifty patients with periodontitis, 25 smokers and 25 non-smokers, and 20 periodontally healthy patients were recruited. After periodontal clinical evaluation, gingival crevicular fluid (GCF) samples were collected from healthy sites of patients without periodontal disease and from healthy and diseased sites of patients with periodontitis. Peptides quantification was performed by sandwich ELISA technique. Smokers showed reduced GCF hBD 1 levels and increased hBD 2 levels compared to non-smokers in diseased sites (p <0.05). Higher levels of hBD 1 were observed in healthy sites of patients without periodontal disease than in healthy sites of patients with periodontitis (p<0.0001). Diseased sites of non-smokers presented higher levels of hBD 2 than healthy sites (p <0.05). These results reveal that protein levels of hBDs 1 and 2 can be impaired by cigarette smoking in the presence of periodontal disease.
publishDate 2022
dc.date.none.fl_str_mv 2022-08-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402022000400079
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402022000400079
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0103-6440202204685
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Fundação Odontológica de Ribeirão Preto
publisher.none.fl_str_mv Fundação Odontológica de Ribeirão Preto
dc.source.none.fl_str_mv Brazilian Dental Journal v.33 n.4 2022
reponame:Brazilian Dental Journal
instname:Fundação Odontológica de Ribeirão Preto (FUNORP)
instacron:FUNORP
instname_str Fundação Odontológica de Ribeirão Preto (FUNORP)
instacron_str FUNORP
institution FUNORP
reponame_str Brazilian Dental Journal
collection Brazilian Dental Journal
repository.name.fl_str_mv Brazilian Dental Journal - Fundação Odontológica de Ribeirão Preto (FUNORP)
repository.mail.fl_str_mv bdj@forp.usp.br||sergio@fosjc.unesp.br
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