Simultaneous extraction of phycobiliproteins and carbonic Anhydrase from Spirulina Platensis Leb-52

Detalhes bibliográficos
Autor(a) principal: Sala, Luisa
Data de Publicação: 2020
Outros Autores: Moraes, Caroline Costa, Kalil, Susana Juliano
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Vetor (Online)
Texto Completo: https://periodicos.furg.br/vetor/article/view/7055
Resumo: C-phycocyanin (C-PC), allophycocyanin (APC) and carbonic anhydrase (CA) are intracellular bioproducts produced by Spirulina platensis LEB-52. In order to extract them, a cell rupture step is required. The influence of extraction time on ultrasound-assisted extraction (UAE) was evaluated at two biomass concentrations (0.2 and 23 g/L). At biomass concentration of 0.2 g/L, 12-min UAE was sufficient to simultaneously obtain phycobiliproteins (C-PC and APC) and esterase-expressed CA. Enzyme activity of hydratase-expressed CA in cyanobacteria is very low. Therefore, to determine the activity of hydratase-expressed CA, biomass concentration of 23 g/L and 8-min extraction time were needed. UAE is an efficient method of rapid determination of phycobiliproteins in wet biomass, even though it is not a selective method which extracts an array of contaminants from biomass.
id FURG-7_8f03bbd04b7454911c3cfc12130645f0
oai_identifier_str oai:periodicos.furg.br:article/7055
network_acronym_str FURG-7
network_name_str Vetor (Online)
repository_id_str
spelling Simultaneous extraction of phycobiliproteins and carbonic Anhydrase from Spirulina Platensis Leb-52C-phycocyaninAllophycocyaninMicroalgaeUltrasound-assisted extractionHydratase.C-phycocyanin (C-PC), allophycocyanin (APC) and carbonic anhydrase (CA) are intracellular bioproducts produced by Spirulina platensis LEB-52. In order to extract them, a cell rupture step is required. The influence of extraction time on ultrasound-assisted extraction (UAE) was evaluated at two biomass concentrations (0.2 and 23 g/L). At biomass concentration of 0.2 g/L, 12-min UAE was sufficient to simultaneously obtain phycobiliproteins (C-PC and APC) and esterase-expressed CA. Enzyme activity of hydratase-expressed CA in cyanobacteria is very low. Therefore, to determine the activity of hydratase-expressed CA, biomass concentration of 23 g/L and 8-min extraction time were needed. UAE is an efficient method of rapid determination of phycobiliproteins in wet biomass, even though it is not a selective method which extracts an array of contaminants from biomass.Universidade Federal do Rio Grande2020-06-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicos.furg.br/vetor/article/view/7055VETOR - Journal of Exact Sciences and Engineering; Vol. 27 No. 1 (2017); 57-74VETOR - Revista de Ciências Exatas e Engenharias; v. 27 n. 1 (2017); 57-742358-34520102-7352reponame:Vetor (Online)instname:Universidade Federal do Rio Grande (FURG)instacron:FURGenghttps://periodicos.furg.br/vetor/article/view/7055/7415Copyright (c) 2020 VETOR - Revista de Ciências Exatas e Engenhariasinfo:eu-repo/semantics/openAccessSala, LuisaMoraes, Caroline CostaKalil, Susana Juliano2020-06-09T00:33:47Zoai:periodicos.furg.br:article/7055Revistahttps://periodicos.furg.br/vetorPUBhttps://periodicos.furg.br/vetor/oaigmplatt@furg.br2358-34520102-7352opendoar:2020-06-09T00:33:47Vetor (Online) - Universidade Federal do Rio Grande (FURG)false
dc.title.none.fl_str_mv Simultaneous extraction of phycobiliproteins and carbonic Anhydrase from Spirulina Platensis Leb-52
title Simultaneous extraction of phycobiliproteins and carbonic Anhydrase from Spirulina Platensis Leb-52
spellingShingle Simultaneous extraction of phycobiliproteins and carbonic Anhydrase from Spirulina Platensis Leb-52
Sala, Luisa
C-phycocyanin
Allophycocyanin
Microalgae
Ultrasound-assisted extraction
Hydratase.
title_short Simultaneous extraction of phycobiliproteins and carbonic Anhydrase from Spirulina Platensis Leb-52
title_full Simultaneous extraction of phycobiliproteins and carbonic Anhydrase from Spirulina Platensis Leb-52
title_fullStr Simultaneous extraction of phycobiliproteins and carbonic Anhydrase from Spirulina Platensis Leb-52
title_full_unstemmed Simultaneous extraction of phycobiliproteins and carbonic Anhydrase from Spirulina Platensis Leb-52
title_sort Simultaneous extraction of phycobiliproteins and carbonic Anhydrase from Spirulina Platensis Leb-52
author Sala, Luisa
author_facet Sala, Luisa
Moraes, Caroline Costa
Kalil, Susana Juliano
author_role author
author2 Moraes, Caroline Costa
Kalil, Susana Juliano
author2_role author
author
dc.contributor.author.fl_str_mv Sala, Luisa
Moraes, Caroline Costa
Kalil, Susana Juliano
dc.subject.por.fl_str_mv C-phycocyanin
Allophycocyanin
Microalgae
Ultrasound-assisted extraction
Hydratase.
topic C-phycocyanin
Allophycocyanin
Microalgae
Ultrasound-assisted extraction
Hydratase.
description C-phycocyanin (C-PC), allophycocyanin (APC) and carbonic anhydrase (CA) are intracellular bioproducts produced by Spirulina platensis LEB-52. In order to extract them, a cell rupture step is required. The influence of extraction time on ultrasound-assisted extraction (UAE) was evaluated at two biomass concentrations (0.2 and 23 g/L). At biomass concentration of 0.2 g/L, 12-min UAE was sufficient to simultaneously obtain phycobiliproteins (C-PC and APC) and esterase-expressed CA. Enzyme activity of hydratase-expressed CA in cyanobacteria is very low. Therefore, to determine the activity of hydratase-expressed CA, biomass concentration of 23 g/L and 8-min extraction time were needed. UAE is an efficient method of rapid determination of phycobiliproteins in wet biomass, even though it is not a selective method which extracts an array of contaminants from biomass.
publishDate 2020
dc.date.none.fl_str_mv 2020-06-08
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://periodicos.furg.br/vetor/article/view/7055
url https://periodicos.furg.br/vetor/article/view/7055
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://periodicos.furg.br/vetor/article/view/7055/7415
dc.rights.driver.fl_str_mv Copyright (c) 2020 VETOR - Revista de Ciências Exatas e Engenharias
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2020 VETOR - Revista de Ciências Exatas e Engenharias
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal do Rio Grande
publisher.none.fl_str_mv Universidade Federal do Rio Grande
dc.source.none.fl_str_mv VETOR - Journal of Exact Sciences and Engineering; Vol. 27 No. 1 (2017); 57-74
VETOR - Revista de Ciências Exatas e Engenharias; v. 27 n. 1 (2017); 57-74
2358-3452
0102-7352
reponame:Vetor (Online)
instname:Universidade Federal do Rio Grande (FURG)
instacron:FURG
instname_str Universidade Federal do Rio Grande (FURG)
instacron_str FURG
institution FURG
reponame_str Vetor (Online)
collection Vetor (Online)
repository.name.fl_str_mv Vetor (Online) - Universidade Federal do Rio Grande (FURG)
repository.mail.fl_str_mv gmplatt@furg.br
_version_ 1797041761747468288

Registros relacionados