Fitness of Mycobacterium tuberculosis strains of the W-Beijing and Non-W-Beijing genotype
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FURG (RI FURG) |
Texto Completo: | http://repositorio.furg.br/handle/1/3973 |
Resumo: | Background: Multidrug resistant tuberculosis (MDR-TB) is a major threat for global tuberculosis control. The W-Beijing Mycobacterium tuberculosis genotype has been associated with drug resistance. Elucidation of the mechanisms underlying this epidemiological finding may have an important role in the control of MDR-TB. The aim of this study was to evaluate the fitness of drug-susceptible and MDR M. tuberculosis strains of the W-Beijing genotype compared with that of Non-W-Beijing strains. Methodology/Principal Findings: Fitness of M. tuberculosis strains was determined by evaluating the difference in the growth curves obtained in the MGIT960 automated system and assessing the competitive growth capacity between WBeijing and non-W-Beijing strains. The W-Beijing MDR strains had a significant longer lag phase duration compared to the other strains but did not present a significant fitness cost. When grown in competition they had an advantage only in medium containing 0.1% Tween 80. Conclusions/Significance: It was not possible to confirm a selective advantage of W-Beijing strains to grow, except for differences in their resistance to Tween 80. Further studies are needed to elucidate the putative advantage of W-Beijing strains compared to other genotypes. |
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Fitness of Mycobacterium tuberculosis strains of the W-Beijing and Non-W-Beijing genotypeBackground: Multidrug resistant tuberculosis (MDR-TB) is a major threat for global tuberculosis control. The W-Beijing Mycobacterium tuberculosis genotype has been associated with drug resistance. Elucidation of the mechanisms underlying this epidemiological finding may have an important role in the control of MDR-TB. The aim of this study was to evaluate the fitness of drug-susceptible and MDR M. tuberculosis strains of the W-Beijing genotype compared with that of Non-W-Beijing strains. Methodology/Principal Findings: Fitness of M. tuberculosis strains was determined by evaluating the difference in the growth curves obtained in the MGIT960 automated system and assessing the competitive growth capacity between WBeijing and non-W-Beijing strains. The W-Beijing MDR strains had a significant longer lag phase duration compared to the other strains but did not present a significant fitness cost. When grown in competition they had an advantage only in medium containing 0.1% Tween 80. Conclusions/Significance: It was not possible to confirm a selective advantage of W-Beijing strains to grow, except for differences in their resistance to Tween 80. Further studies are needed to elucidate the putative advantage of W-Beijing strains compared to other genotypes.2013-10-12T02:23:36Z2013-10-12T02:23:36Z2010info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfGROLL, Andrea Von et al. Fitness of Mycobacterium tuberculosis strains of the W-Beijing and Non-W-Beijing genotype. Plos One, v. 5, n. 4, p. 1-6, 2010. Disponível em: <http://www.plosone.org/article/fetchObject.action?uri=info%3Adoi%2F10.1371%2Fjournal.pone.0010191&representation=PDF>. Acesso em: 17 set. 2012.http://repositorio.furg.br/handle/1/3973engGroll, Andrea VonMartin, AnandiStehr, MatthiasSingh, MahavirPortaels, FrançoiseSilva, Pedro Eduardo Almeida daPalomino, Juan Carlosinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FURG (RI FURG)instname:Universidade Federal do Rio Grande (FURG)instacron:FURG2013-10-12T02:23:36Zoai:repositorio.furg.br:1/3973Repositório InstitucionalPUBhttps://repositorio.furg.br/oai/request || http://200.19.254.174/oai/requestopendoar:2013-10-12T02:23:36Repositório Institucional da FURG (RI FURG) - Universidade Federal do Rio Grande (FURG)false |
dc.title.none.fl_str_mv |
Fitness of Mycobacterium tuberculosis strains of the W-Beijing and Non-W-Beijing genotype |
title |
Fitness of Mycobacterium tuberculosis strains of the W-Beijing and Non-W-Beijing genotype |
spellingShingle |
Fitness of Mycobacterium tuberculosis strains of the W-Beijing and Non-W-Beijing genotype Groll, Andrea Von |
title_short |
Fitness of Mycobacterium tuberculosis strains of the W-Beijing and Non-W-Beijing genotype |
title_full |
Fitness of Mycobacterium tuberculosis strains of the W-Beijing and Non-W-Beijing genotype |
title_fullStr |
Fitness of Mycobacterium tuberculosis strains of the W-Beijing and Non-W-Beijing genotype |
title_full_unstemmed |
Fitness of Mycobacterium tuberculosis strains of the W-Beijing and Non-W-Beijing genotype |
title_sort |
Fitness of Mycobacterium tuberculosis strains of the W-Beijing and Non-W-Beijing genotype |
author |
Groll, Andrea Von |
author_facet |
Groll, Andrea Von Martin, Anandi Stehr, Matthias Singh, Mahavir Portaels, Françoise Silva, Pedro Eduardo Almeida da Palomino, Juan Carlos |
author_role |
author |
author2 |
Martin, Anandi Stehr, Matthias Singh, Mahavir Portaels, Françoise Silva, Pedro Eduardo Almeida da Palomino, Juan Carlos |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Groll, Andrea Von Martin, Anandi Stehr, Matthias Singh, Mahavir Portaels, Françoise Silva, Pedro Eduardo Almeida da Palomino, Juan Carlos |
description |
Background: Multidrug resistant tuberculosis (MDR-TB) is a major threat for global tuberculosis control. The W-Beijing Mycobacterium tuberculosis genotype has been associated with drug resistance. Elucidation of the mechanisms underlying this epidemiological finding may have an important role in the control of MDR-TB. The aim of this study was to evaluate the fitness of drug-susceptible and MDR M. tuberculosis strains of the W-Beijing genotype compared with that of Non-W-Beijing strains. Methodology/Principal Findings: Fitness of M. tuberculosis strains was determined by evaluating the difference in the growth curves obtained in the MGIT960 automated system and assessing the competitive growth capacity between WBeijing and non-W-Beijing strains. The W-Beijing MDR strains had a significant longer lag phase duration compared to the other strains but did not present a significant fitness cost. When grown in competition they had an advantage only in medium containing 0.1% Tween 80. Conclusions/Significance: It was not possible to confirm a selective advantage of W-Beijing strains to grow, except for differences in their resistance to Tween 80. Further studies are needed to elucidate the putative advantage of W-Beijing strains compared to other genotypes. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010 2013-10-12T02:23:36Z 2013-10-12T02:23:36Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
GROLL, Andrea Von et al. Fitness of Mycobacterium tuberculosis strains of the W-Beijing and Non-W-Beijing genotype. Plos One, v. 5, n. 4, p. 1-6, 2010. Disponível em: <http://www.plosone.org/article/fetchObject.action?uri=info%3Adoi%2F10.1371%2Fjournal.pone.0010191&representation=PDF>. Acesso em: 17 set. 2012. http://repositorio.furg.br/handle/1/3973 |
identifier_str_mv |
GROLL, Andrea Von et al. Fitness of Mycobacterium tuberculosis strains of the W-Beijing and Non-W-Beijing genotype. Plos One, v. 5, n. 4, p. 1-6, 2010. Disponível em: <http://www.plosone.org/article/fetchObject.action?uri=info%3Adoi%2F10.1371%2Fjournal.pone.0010191&representation=PDF>. Acesso em: 17 set. 2012. |
url |
http://repositorio.furg.br/handle/1/3973 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
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reponame:Repositório Institucional da FURG (RI FURG) instname:Universidade Federal do Rio Grande (FURG) instacron:FURG |
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Universidade Federal do Rio Grande (FURG) |
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FURG |
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FURG |
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Repositório Institucional da FURG (RI FURG) |
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Repositório Institucional da FURG (RI FURG) |
repository.name.fl_str_mv |
Repositório Institucional da FURG (RI FURG) - Universidade Federal do Rio Grande (FURG) |
repository.mail.fl_str_mv |
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1813187242394386432 |