Fitness of Mycobacterium tuberculosis strains of the W-Beijing and Non-W-Beijing genotype

Detalhes bibliográficos
Autor(a) principal: Groll, Andrea Von
Data de Publicação: 2010
Outros Autores: Martin, Anandi, Stehr, Matthias, Singh, Mahavir, Portaels, Françoise, Silva, Pedro Eduardo Almeida da, Palomino, Juan Carlos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FURG (RI FURG)
Texto Completo: http://repositorio.furg.br/handle/1/3973
Resumo: Background: Multidrug resistant tuberculosis (MDR-TB) is a major threat for global tuberculosis control. The W-Beijing Mycobacterium tuberculosis genotype has been associated with drug resistance. Elucidation of the mechanisms underlying this epidemiological finding may have an important role in the control of MDR-TB. The aim of this study was to evaluate the fitness of drug-susceptible and MDR M. tuberculosis strains of the W-Beijing genotype compared with that of Non-W-Beijing strains. Methodology/Principal Findings: Fitness of M. tuberculosis strains was determined by evaluating the difference in the growth curves obtained in the MGIT960 automated system and assessing the competitive growth capacity between WBeijing and non-W-Beijing strains. The W-Beijing MDR strains had a significant longer lag phase duration compared to the other strains but did not present a significant fitness cost. When grown in competition they had an advantage only in medium containing 0.1% Tween 80. Conclusions/Significance: It was not possible to confirm a selective advantage of W-Beijing strains to grow, except for differences in their resistance to Tween 80. Further studies are needed to elucidate the putative advantage of W-Beijing strains compared to other genotypes.
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spelling Fitness of Mycobacterium tuberculosis strains of the W-Beijing and Non-W-Beijing genotypeBackground: Multidrug resistant tuberculosis (MDR-TB) is a major threat for global tuberculosis control. The W-Beijing Mycobacterium tuberculosis genotype has been associated with drug resistance. Elucidation of the mechanisms underlying this epidemiological finding may have an important role in the control of MDR-TB. The aim of this study was to evaluate the fitness of drug-susceptible and MDR M. tuberculosis strains of the W-Beijing genotype compared with that of Non-W-Beijing strains. Methodology/Principal Findings: Fitness of M. tuberculosis strains was determined by evaluating the difference in the growth curves obtained in the MGIT960 automated system and assessing the competitive growth capacity between WBeijing and non-W-Beijing strains. The W-Beijing MDR strains had a significant longer lag phase duration compared to the other strains but did not present a significant fitness cost. When grown in competition they had an advantage only in medium containing 0.1% Tween 80. Conclusions/Significance: It was not possible to confirm a selective advantage of W-Beijing strains to grow, except for differences in their resistance to Tween 80. Further studies are needed to elucidate the putative advantage of W-Beijing strains compared to other genotypes.2013-10-12T02:23:36Z2013-10-12T02:23:36Z2010info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfGROLL, Andrea Von et al. Fitness of Mycobacterium tuberculosis strains of the W-Beijing and Non-W-Beijing genotype. Plos One, v. 5, n. 4, p. 1-6, 2010. Disponível em: <http://www.plosone.org/article/fetchObject.action?uri=info%3Adoi%2F10.1371%2Fjournal.pone.0010191&representation=PDF>. Acesso em: 17 set. 2012.http://repositorio.furg.br/handle/1/3973engGroll, Andrea VonMartin, AnandiStehr, MatthiasSingh, MahavirPortaels, FrançoiseSilva, Pedro Eduardo Almeida daPalomino, Juan Carlosinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FURG (RI FURG)instname:Universidade Federal do Rio Grande (FURG)instacron:FURG2013-10-12T02:23:36Zoai:repositorio.furg.br:1/3973Repositório InstitucionalPUBhttps://repositorio.furg.br/oai/request || http://200.19.254.174/oai/requestopendoar:2013-10-12T02:23:36Repositório Institucional da FURG (RI FURG) - Universidade Federal do Rio Grande (FURG)false
dc.title.none.fl_str_mv Fitness of Mycobacterium tuberculosis strains of the W-Beijing and Non-W-Beijing genotype
title Fitness of Mycobacterium tuberculosis strains of the W-Beijing and Non-W-Beijing genotype
spellingShingle Fitness of Mycobacterium tuberculosis strains of the W-Beijing and Non-W-Beijing genotype
Groll, Andrea Von
title_short Fitness of Mycobacterium tuberculosis strains of the W-Beijing and Non-W-Beijing genotype
title_full Fitness of Mycobacterium tuberculosis strains of the W-Beijing and Non-W-Beijing genotype
title_fullStr Fitness of Mycobacterium tuberculosis strains of the W-Beijing and Non-W-Beijing genotype
title_full_unstemmed Fitness of Mycobacterium tuberculosis strains of the W-Beijing and Non-W-Beijing genotype
title_sort Fitness of Mycobacterium tuberculosis strains of the W-Beijing and Non-W-Beijing genotype
author Groll, Andrea Von
author_facet Groll, Andrea Von
Martin, Anandi
Stehr, Matthias
Singh, Mahavir
Portaels, Françoise
Silva, Pedro Eduardo Almeida da
Palomino, Juan Carlos
author_role author
author2 Martin, Anandi
Stehr, Matthias
Singh, Mahavir
Portaels, Françoise
Silva, Pedro Eduardo Almeida da
Palomino, Juan Carlos
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Groll, Andrea Von
Martin, Anandi
Stehr, Matthias
Singh, Mahavir
Portaels, Françoise
Silva, Pedro Eduardo Almeida da
Palomino, Juan Carlos
description Background: Multidrug resistant tuberculosis (MDR-TB) is a major threat for global tuberculosis control. The W-Beijing Mycobacterium tuberculosis genotype has been associated with drug resistance. Elucidation of the mechanisms underlying this epidemiological finding may have an important role in the control of MDR-TB. The aim of this study was to evaluate the fitness of drug-susceptible and MDR M. tuberculosis strains of the W-Beijing genotype compared with that of Non-W-Beijing strains. Methodology/Principal Findings: Fitness of M. tuberculosis strains was determined by evaluating the difference in the growth curves obtained in the MGIT960 automated system and assessing the competitive growth capacity between WBeijing and non-W-Beijing strains. The W-Beijing MDR strains had a significant longer lag phase duration compared to the other strains but did not present a significant fitness cost. When grown in competition they had an advantage only in medium containing 0.1% Tween 80. Conclusions/Significance: It was not possible to confirm a selective advantage of W-Beijing strains to grow, except for differences in their resistance to Tween 80. Further studies are needed to elucidate the putative advantage of W-Beijing strains compared to other genotypes.
publishDate 2010
dc.date.none.fl_str_mv 2010
2013-10-12T02:23:36Z
2013-10-12T02:23:36Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.uri.fl_str_mv GROLL, Andrea Von et al. Fitness of Mycobacterium tuberculosis strains of the W-Beijing and Non-W-Beijing genotype. Plos One, v. 5, n. 4, p. 1-6, 2010. Disponível em: <http://www.plosone.org/article/fetchObject.action?uri=info%3Adoi%2F10.1371%2Fjournal.pone.0010191&representation=PDF>. Acesso em: 17 set. 2012.
http://repositorio.furg.br/handle/1/3973
identifier_str_mv GROLL, Andrea Von et al. Fitness of Mycobacterium tuberculosis strains of the W-Beijing and Non-W-Beijing genotype. Plos One, v. 5, n. 4, p. 1-6, 2010. Disponível em: <http://www.plosone.org/article/fetchObject.action?uri=info%3Adoi%2F10.1371%2Fjournal.pone.0010191&representation=PDF>. Acesso em: 17 set. 2012.
url http://repositorio.furg.br/handle/1/3973
dc.language.iso.fl_str_mv eng
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instname_str Universidade Federal do Rio Grande (FURG)
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