Production of a rhamnolipid-type biosurfactant by Pseudomonas aeruginosa LBM10 grown on glycerol

Detalhes bibliográficos
Autor(a) principal: Rosa, Célia Francisca Centeno da
Data de Publicação: 2010
Outros Autores: Michelon, Mariano, Burkert, Janaina Fernandes de Medeiros, Kalil, Susana Juliano, Burkert, Carlos André Veiga
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FURG (RI FURG)
Texto Completo: http://repositorio.furg.br/handle/1/1841
Resumo: The work herewith investigated the effect of the culture medium composition on rhamnolipid production by Pseudomonas aeruginosa LBM10, previously isolated from an estuarine environment in Southern Brazil. Experimental design and surface response methodology were used in order to improve biosurfactant production using glycerol, a renewable carbon source. The assays were carried out in a rotary shaker at 30°C and 180 rpm for 120 h and the parameters studied were glycerol concentration, C/N (carbon/nitrogen) and C/P (carbon/phosphorus) ratios. Low glycerol concentration and a phosphorus-limiting condition were favorable for rhamnolipid production. Contour plots constructed by predictive polynomial equations led to a glycerol concentration of 13.2 g/l, a C/N ratio of 12.8 and a C/P ratio of 40 in order to maximize rhamnolipid concentration (4.15 g/l) associated with a high emulsification index (61%).
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spelling Production of a rhamnolipid-type biosurfactant by Pseudomonas aeruginosa LBM10 grown on glycerolBiosurfactantSurface-active compoundsExperimental designPhosphorus limitationThe work herewith investigated the effect of the culture medium composition on rhamnolipid production by Pseudomonas aeruginosa LBM10, previously isolated from an estuarine environment in Southern Brazil. Experimental design and surface response methodology were used in order to improve biosurfactant production using glycerol, a renewable carbon source. The assays were carried out in a rotary shaker at 30°C and 180 rpm for 120 h and the parameters studied were glycerol concentration, C/N (carbon/nitrogen) and C/P (carbon/phosphorus) ratios. Low glycerol concentration and a phosphorus-limiting condition were favorable for rhamnolipid production. Contour plots constructed by predictive polynomial equations led to a glycerol concentration of 13.2 g/l, a C/N ratio of 12.8 and a C/P ratio of 40 in order to maximize rhamnolipid concentration (4.15 g/l) associated with a high emulsification index (61%).2012-03-06T03:52:58Z2012-03-06T03:52:58Z2010info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfROSA, Célia Francisca Centeno da et al. Production of a rhamnolipid-type biosurfactant by Pseudomonas aeruginosa LBM10 grown on glycerol. African Journal of Biotechnology, v. 9, n. 40, p. 9012-9017, 2010. Disponível em: <http://www.academicjournals.org/AJB/PDF/pdf2010/27Dec/da%20Rosa%20et%20al.pdf>. Acesso em: 20 dez. de 2011.1684–5315http://repositorio.furg.br/handle/1/1841porRosa, Célia Francisca Centeno daMichelon, MarianoBurkert, Janaina Fernandes de MedeirosKalil, Susana JulianoBurkert, Carlos André Veigainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FURG (RI FURG)instname:Universidade Federal do Rio Grande (FURG)instacron:FURG2014-08-29T17:58:17Zoai:repositorio.furg.br:1/1841Repositório InstitucionalPUBhttps://repositorio.furg.br/oai/request || http://200.19.254.174/oai/requestopendoar:2014-08-29T17:58:17Repositório Institucional da FURG (RI FURG) - Universidade Federal do Rio Grande (FURG)false
dc.title.none.fl_str_mv Production of a rhamnolipid-type biosurfactant by Pseudomonas aeruginosa LBM10 grown on glycerol
title Production of a rhamnolipid-type biosurfactant by Pseudomonas aeruginosa LBM10 grown on glycerol
spellingShingle Production of a rhamnolipid-type biosurfactant by Pseudomonas aeruginosa LBM10 grown on glycerol
Rosa, Célia Francisca Centeno da
Biosurfactant
Surface-active compounds
Experimental design
Phosphorus limitation
title_short Production of a rhamnolipid-type biosurfactant by Pseudomonas aeruginosa LBM10 grown on glycerol
title_full Production of a rhamnolipid-type biosurfactant by Pseudomonas aeruginosa LBM10 grown on glycerol
title_fullStr Production of a rhamnolipid-type biosurfactant by Pseudomonas aeruginosa LBM10 grown on glycerol
title_full_unstemmed Production of a rhamnolipid-type biosurfactant by Pseudomonas aeruginosa LBM10 grown on glycerol
title_sort Production of a rhamnolipid-type biosurfactant by Pseudomonas aeruginosa LBM10 grown on glycerol
author Rosa, Célia Francisca Centeno da
author_facet Rosa, Célia Francisca Centeno da
Michelon, Mariano
Burkert, Janaina Fernandes de Medeiros
Kalil, Susana Juliano
Burkert, Carlos André Veiga
author_role author
author2 Michelon, Mariano
Burkert, Janaina Fernandes de Medeiros
Kalil, Susana Juliano
Burkert, Carlos André Veiga
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Rosa, Célia Francisca Centeno da
Michelon, Mariano
Burkert, Janaina Fernandes de Medeiros
Kalil, Susana Juliano
Burkert, Carlos André Veiga
dc.subject.por.fl_str_mv Biosurfactant
Surface-active compounds
Experimental design
Phosphorus limitation
topic Biosurfactant
Surface-active compounds
Experimental design
Phosphorus limitation
description The work herewith investigated the effect of the culture medium composition on rhamnolipid production by Pseudomonas aeruginosa LBM10, previously isolated from an estuarine environment in Southern Brazil. Experimental design and surface response methodology were used in order to improve biosurfactant production using glycerol, a renewable carbon source. The assays were carried out in a rotary shaker at 30°C and 180 rpm for 120 h and the parameters studied were glycerol concentration, C/N (carbon/nitrogen) and C/P (carbon/phosphorus) ratios. Low glycerol concentration and a phosphorus-limiting condition were favorable for rhamnolipid production. Contour plots constructed by predictive polynomial equations led to a glycerol concentration of 13.2 g/l, a C/N ratio of 12.8 and a C/P ratio of 40 in order to maximize rhamnolipid concentration (4.15 g/l) associated with a high emulsification index (61%).
publishDate 2010
dc.date.none.fl_str_mv 2010
2012-03-06T03:52:58Z
2012-03-06T03:52:58Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv ROSA, Célia Francisca Centeno da et al. Production of a rhamnolipid-type biosurfactant by Pseudomonas aeruginosa LBM10 grown on glycerol. African Journal of Biotechnology, v. 9, n. 40, p. 9012-9017, 2010. Disponível em: <http://www.academicjournals.org/AJB/PDF/pdf2010/27Dec/da%20Rosa%20et%20al.pdf>. Acesso em: 20 dez. de 2011.
1684–5315
http://repositorio.furg.br/handle/1/1841
identifier_str_mv ROSA, Célia Francisca Centeno da et al. Production of a rhamnolipid-type biosurfactant by Pseudomonas aeruginosa LBM10 grown on glycerol. African Journal of Biotechnology, v. 9, n. 40, p. 9012-9017, 2010. Disponível em: <http://www.academicjournals.org/AJB/PDF/pdf2010/27Dec/da%20Rosa%20et%20al.pdf>. Acesso em: 20 dez. de 2011.
1684–5315
url http://repositorio.furg.br/handle/1/1841
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Institucional da FURG (RI FURG)
instname:Universidade Federal do Rio Grande (FURG)
instacron:FURG
instname_str Universidade Federal do Rio Grande (FURG)
instacron_str FURG
institution FURG
reponame_str Repositório Institucional da FURG (RI FURG)
collection Repositório Institucional da FURG (RI FURG)
repository.name.fl_str_mv Repositório Institucional da FURG (RI FURG) - Universidade Federal do Rio Grande (FURG)
repository.mail.fl_str_mv
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