Evaluation of the speed-oligo mycobacteria assay for the identification of nontuberculous mycobacteria
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FURG (RI FURG) |
Texto Completo: | http://repositorio.furg.br/handle/1/7022 |
Resumo: | Nontuberculous mycobacteria (NTM) causing human infectious disease have become increasingly common. Rapid and accurate identification to the species level is, therefore, critical. The Speed-Oligo Mycobacteria assay is an oligochromatographic method that was made available recently for the identification and differentiation of mycobacteria. The present study aimed to evaluate the performance of the Speed-Oligo Mycobacteria assay for the identification of NTM. We examined a total of 62 strains (9 type strains, 19 reference strains and 34 clinical isolates) belonging to 13 different species (Mycobacterium intracellulare, M. fortuitum, M. gordonae, M. kansasii, M. marinum, M. peregrinum, M. scrofulaceum, M. abscessus, M. bovis BCG, M. chelonae, M. avium, M. malmoense and M. xenopi). The Speed-Oligo Mycobacteria assay was performed according to the manufacturer’s instructions. Discrepant results between Speed-Oligo Mycobacteria and the original identification were reassessed by the Speed-Oligo Mycobacteria assay and resolved by the GenoType Mycobacterium CM assay and by sequencing of 16S rRNA and protein-encoding genes. We found 93.5 % (58/62) concordance for the identification of NTM as compared with the original identification. Three strains were erroneously identified by Speed-Oligo Mycobacteria: one M. kansasii strain was identified as Mycobacterium tuberculosis complex, and one M. chelonae strain and one M. peregrinum strain were both identified as Mycobacterium abscessus. Moreover, one M. chelonae strain was not identified by Speed-Oligo Mycobacteria since it did not react with any species-specific probe. For these strains, sequencing of the genes hsp65, 16S rRNA and rpoB and the GenoType Mycobacterium CM assay were performed. The Speed-Oligo Mycobacteria assay can be a useful tool for the rapid and easy identification of the most common NTM. If applied in clinical practice it could reduce diagnostic delays and contribute to correct clinical and better management of infections caused by NTM. |
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Evaluation of the speed-oligo mycobacteria assay for the identification of nontuberculous mycobacteriaNontuberculous mycobacteriaNontuberculous mycobacteria (NTM) causing human infectious disease have become increasingly common. Rapid and accurate identification to the species level is, therefore, critical. The Speed-Oligo Mycobacteria assay is an oligochromatographic method that was made available recently for the identification and differentiation of mycobacteria. The present study aimed to evaluate the performance of the Speed-Oligo Mycobacteria assay for the identification of NTM. We examined a total of 62 strains (9 type strains, 19 reference strains and 34 clinical isolates) belonging to 13 different species (Mycobacterium intracellulare, M. fortuitum, M. gordonae, M. kansasii, M. marinum, M. peregrinum, M. scrofulaceum, M. abscessus, M. bovis BCG, M. chelonae, M. avium, M. malmoense and M. xenopi). The Speed-Oligo Mycobacteria assay was performed according to the manufacturer’s instructions. Discrepant results between Speed-Oligo Mycobacteria and the original identification were reassessed by the Speed-Oligo Mycobacteria assay and resolved by the GenoType Mycobacterium CM assay and by sequencing of 16S rRNA and protein-encoding genes. We found 93.5 % (58/62) concordance for the identification of NTM as compared with the original identification. Three strains were erroneously identified by Speed-Oligo Mycobacteria: one M. kansasii strain was identified as Mycobacterium tuberculosis complex, and one M. chelonae strain and one M. peregrinum strain were both identified as Mycobacterium abscessus. Moreover, one M. chelonae strain was not identified by Speed-Oligo Mycobacteria since it did not react with any species-specific probe. For these strains, sequencing of the genes hsp65, 16S rRNA and rpoB and the GenoType Mycobacterium CM assay were performed. The Speed-Oligo Mycobacteria assay can be a useful tool for the rapid and easy identification of the most common NTM. If applied in clinical practice it could reduce diagnostic delays and contribute to correct clinical and better management of infections caused by NTM.2017-01-16T17:40:23Z2017-01-16T17:40:23Z2015info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfRAMIS, Ivy Bastos; et al. Evaluation of the speed-oligo mycobacteria assay for the identification of nontuberculous mycobacteria. Journal of Medical Microbiology. V. 64, p. 283–287, 2015. Disponível em: < http://jmm.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.000025>. Acesso em: 12 jan. 2017.http://repositorio.furg.br/handle/1/7022DOI 10.1099/jmm.0.000025engRamis, Ivy BastosCnockaert, MargoGroll, Andrea VonMathys, VanessaSimon, AnneTortoli, EnricoPalomino, Juan CarlosSilva, Pedro Eduardo Almeida daVandamme, PeterAndre, EmmanuelMartin, Anandiinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FURG (RI FURG)instname:Universidade Federal do Rio Grande (FURG)instacron:FURG2017-01-16T17:40:23Zoai:repositorio.furg.br:1/7022Repositório InstitucionalPUBhttps://repositorio.furg.br/oai/request || http://200.19.254.174/oai/requestopendoar:2017-01-16T17:40:23Repositório Institucional da FURG (RI FURG) - Universidade Federal do Rio Grande (FURG)false |
dc.title.none.fl_str_mv |
Evaluation of the speed-oligo mycobacteria assay for the identification of nontuberculous mycobacteria |
title |
Evaluation of the speed-oligo mycobacteria assay for the identification of nontuberculous mycobacteria |
spellingShingle |
Evaluation of the speed-oligo mycobacteria assay for the identification of nontuberculous mycobacteria Ramis, Ivy Bastos Nontuberculous mycobacteria |
title_short |
Evaluation of the speed-oligo mycobacteria assay for the identification of nontuberculous mycobacteria |
title_full |
Evaluation of the speed-oligo mycobacteria assay for the identification of nontuberculous mycobacteria |
title_fullStr |
Evaluation of the speed-oligo mycobacteria assay for the identification of nontuberculous mycobacteria |
title_full_unstemmed |
Evaluation of the speed-oligo mycobacteria assay for the identification of nontuberculous mycobacteria |
title_sort |
Evaluation of the speed-oligo mycobacteria assay for the identification of nontuberculous mycobacteria |
author |
Ramis, Ivy Bastos |
author_facet |
Ramis, Ivy Bastos Cnockaert, Margo Groll, Andrea Von Mathys, Vanessa Simon, Anne Tortoli, Enrico Palomino, Juan Carlos Silva, Pedro Eduardo Almeida da Vandamme, Peter Andre, Emmanuel Martin, Anandi |
author_role |
author |
author2 |
Cnockaert, Margo Groll, Andrea Von Mathys, Vanessa Simon, Anne Tortoli, Enrico Palomino, Juan Carlos Silva, Pedro Eduardo Almeida da Vandamme, Peter Andre, Emmanuel Martin, Anandi |
author2_role |
author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Ramis, Ivy Bastos Cnockaert, Margo Groll, Andrea Von Mathys, Vanessa Simon, Anne Tortoli, Enrico Palomino, Juan Carlos Silva, Pedro Eduardo Almeida da Vandamme, Peter Andre, Emmanuel Martin, Anandi |
dc.subject.por.fl_str_mv |
Nontuberculous mycobacteria |
topic |
Nontuberculous mycobacteria |
description |
Nontuberculous mycobacteria (NTM) causing human infectious disease have become increasingly common. Rapid and accurate identification to the species level is, therefore, critical. The Speed-Oligo Mycobacteria assay is an oligochromatographic method that was made available recently for the identification and differentiation of mycobacteria. The present study aimed to evaluate the performance of the Speed-Oligo Mycobacteria assay for the identification of NTM. We examined a total of 62 strains (9 type strains, 19 reference strains and 34 clinical isolates) belonging to 13 different species (Mycobacterium intracellulare, M. fortuitum, M. gordonae, M. kansasii, M. marinum, M. peregrinum, M. scrofulaceum, M. abscessus, M. bovis BCG, M. chelonae, M. avium, M. malmoense and M. xenopi). The Speed-Oligo Mycobacteria assay was performed according to the manufacturer’s instructions. Discrepant results between Speed-Oligo Mycobacteria and the original identification were reassessed by the Speed-Oligo Mycobacteria assay and resolved by the GenoType Mycobacterium CM assay and by sequencing of 16S rRNA and protein-encoding genes. We found 93.5 % (58/62) concordance for the identification of NTM as compared with the original identification. Three strains were erroneously identified by Speed-Oligo Mycobacteria: one M. kansasii strain was identified as Mycobacterium tuberculosis complex, and one M. chelonae strain and one M. peregrinum strain were both identified as Mycobacterium abscessus. Moreover, one M. chelonae strain was not identified by Speed-Oligo Mycobacteria since it did not react with any species-specific probe. For these strains, sequencing of the genes hsp65, 16S rRNA and rpoB and the GenoType Mycobacterium CM assay were performed. The Speed-Oligo Mycobacteria assay can be a useful tool for the rapid and easy identification of the most common NTM. If applied in clinical practice it could reduce diagnostic delays and contribute to correct clinical and better management of infections caused by NTM. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015 2017-01-16T17:40:23Z 2017-01-16T17:40:23Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
RAMIS, Ivy Bastos; et al. Evaluation of the speed-oligo mycobacteria assay for the identification of nontuberculous mycobacteria. Journal of Medical Microbiology. V. 64, p. 283–287, 2015. Disponível em: < http://jmm.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.000025>. Acesso em: 12 jan. 2017. http://repositorio.furg.br/handle/1/7022 DOI 10.1099/jmm.0.000025 |
identifier_str_mv |
RAMIS, Ivy Bastos; et al. Evaluation of the speed-oligo mycobacteria assay for the identification of nontuberculous mycobacteria. Journal of Medical Microbiology. V. 64, p. 283–287, 2015. Disponível em: < http://jmm.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.000025>. Acesso em: 12 jan. 2017. DOI 10.1099/jmm.0.000025 |
url |
http://repositorio.furg.br/handle/1/7022 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da FURG (RI FURG) instname:Universidade Federal do Rio Grande (FURG) instacron:FURG |
instname_str |
Universidade Federal do Rio Grande (FURG) |
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FURG |
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FURG |
reponame_str |
Repositório Institucional da FURG (RI FURG) |
collection |
Repositório Institucional da FURG (RI FURG) |
repository.name.fl_str_mv |
Repositório Institucional da FURG (RI FURG) - Universidade Federal do Rio Grande (FURG) |
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1813187278989688832 |