Effects of seasonality and moult cycle on the proliferation of nerve cells and on the labelling of ecdysone receptors in an estuarine crab
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FURG (RI FURG) |
Texto Completo: | http://repositorio.furg.br/handle/1/2910 |
Resumo: | Decapod crustaceans show proliferation of the nerve cells in the olfactory lobe throughout their lives. However, the regulation of this process is still poorly understood, since it may vary with endogenous and exogenous factors. The objective of the present investigation was to quantify the proliferation of nerve cells and number of nerve cells with ecdysone receptors in the clusters of the central olfactory system in Neohelice granulata, according to moult stages and in different seasons (summer and winter). Three injections of bromodeoxyuridine (BrdU) were administered to the crabs. Brains were sectioned by microtome and fixed on slides for immunohistochemistry with anti-BrdU and anti-EcR antibodies. The proliferation of nerve cells was higher in winter than in summer, probably because in winter the crabs do not breed and the premoult and postmoult periods are longer. Crabs in postmoult exhibited more BrdU-labelled cells than crabs in premoult or intermoult in winter, because of a greater number of mitoses related to an increase in body size and addition of olfactory receptor neurons. The number of EcRlabelled cells was higher in premoult than in postmoult or intermoult in winter. The proliferation of nerve cells is regulated seasonally and according to moult stages. |
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Effects of seasonality and moult cycle on the proliferation of nerve cells and on the labelling of ecdysone receptors in an estuarine crabProliferation of nerve cellsEcdysone receptorNeohelice granulataSeasonalityMoultDecapod crustaceans show proliferation of the nerve cells in the olfactory lobe throughout their lives. However, the regulation of this process is still poorly understood, since it may vary with endogenous and exogenous factors. The objective of the present investigation was to quantify the proliferation of nerve cells and number of nerve cells with ecdysone receptors in the clusters of the central olfactory system in Neohelice granulata, according to moult stages and in different seasons (summer and winter). Three injections of bromodeoxyuridine (BrdU) were administered to the crabs. Brains were sectioned by microtome and fixed on slides for immunohistochemistry with anti-BrdU and anti-EcR antibodies. The proliferation of nerve cells was higher in winter than in summer, probably because in winter the crabs do not breed and the premoult and postmoult periods are longer. Crabs in postmoult exhibited more BrdU-labelled cells than crabs in premoult or intermoult in winter, because of a greater number of mitoses related to an increase in body size and addition of olfactory receptor neurons. The number of EcRlabelled cells was higher in premoult than in postmoult or intermoult in winter. The proliferation of nerve cells is regulated seasonally and according to moult stages.2012-12-01T01:27:37Z2012-12-01T01:27:37Z2011info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfHOLLMANN, Gabriela et al. Effects of seasonality and moult cycle on the proliferation of nerve cells and on the labelling of ecdysone receptors in an estuarine crab. Journal of comparative physiology, v. 197, p. 293-300, 2011. Disponível em: <http://download.springer.com/static/pdf/857/art%253A10.1007%252Fs00359-010-0611-8.pdf?auth66=1354061243_6efef69c861c60c3b23240976f332ce4&ext=.pdf>. Acesso em: 27 nov. 2012.1432-1351http://repositorio.furg.br/handle/1/291010.1007/s00359-010-0611-8engHollmann, GabrielaFonseca, Duane Barros daAllodi, SilvanaMartínez, Pablo ElíasNery, Luiz Eduardo Maiainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FURG (RI FURG)instname:Universidade Federal do Rio Grande (FURG)instacron:FURG2012-12-01T01:27:37Zoai:repositorio.furg.br:1/2910Repositório InstitucionalPUBhttps://repositorio.furg.br/oai/request || http://200.19.254.174/oai/requestopendoar:2012-12-01T01:27:37Repositório Institucional da FURG (RI FURG) - Universidade Federal do Rio Grande (FURG)false |
dc.title.none.fl_str_mv |
Effects of seasonality and moult cycle on the proliferation of nerve cells and on the labelling of ecdysone receptors in an estuarine crab |
title |
Effects of seasonality and moult cycle on the proliferation of nerve cells and on the labelling of ecdysone receptors in an estuarine crab |
spellingShingle |
Effects of seasonality and moult cycle on the proliferation of nerve cells and on the labelling of ecdysone receptors in an estuarine crab Hollmann, Gabriela Proliferation of nerve cells Ecdysone receptor Neohelice granulata Seasonality Moult |
title_short |
Effects of seasonality and moult cycle on the proliferation of nerve cells and on the labelling of ecdysone receptors in an estuarine crab |
title_full |
Effects of seasonality and moult cycle on the proliferation of nerve cells and on the labelling of ecdysone receptors in an estuarine crab |
title_fullStr |
Effects of seasonality and moult cycle on the proliferation of nerve cells and on the labelling of ecdysone receptors in an estuarine crab |
title_full_unstemmed |
Effects of seasonality and moult cycle on the proliferation of nerve cells and on the labelling of ecdysone receptors in an estuarine crab |
title_sort |
Effects of seasonality and moult cycle on the proliferation of nerve cells and on the labelling of ecdysone receptors in an estuarine crab |
author |
Hollmann, Gabriela |
author_facet |
Hollmann, Gabriela Fonseca, Duane Barros da Allodi, Silvana Martínez, Pablo Elías Nery, Luiz Eduardo Maia |
author_role |
author |
author2 |
Fonseca, Duane Barros da Allodi, Silvana Martínez, Pablo Elías Nery, Luiz Eduardo Maia |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Hollmann, Gabriela Fonseca, Duane Barros da Allodi, Silvana Martínez, Pablo Elías Nery, Luiz Eduardo Maia |
dc.subject.por.fl_str_mv |
Proliferation of nerve cells Ecdysone receptor Neohelice granulata Seasonality Moult |
topic |
Proliferation of nerve cells Ecdysone receptor Neohelice granulata Seasonality Moult |
description |
Decapod crustaceans show proliferation of the nerve cells in the olfactory lobe throughout their lives. However, the regulation of this process is still poorly understood, since it may vary with endogenous and exogenous factors. The objective of the present investigation was to quantify the proliferation of nerve cells and number of nerve cells with ecdysone receptors in the clusters of the central olfactory system in Neohelice granulata, according to moult stages and in different seasons (summer and winter). Three injections of bromodeoxyuridine (BrdU) were administered to the crabs. Brains were sectioned by microtome and fixed on slides for immunohistochemistry with anti-BrdU and anti-EcR antibodies. The proliferation of nerve cells was higher in winter than in summer, probably because in winter the crabs do not breed and the premoult and postmoult periods are longer. Crabs in postmoult exhibited more BrdU-labelled cells than crabs in premoult or intermoult in winter, because of a greater number of mitoses related to an increase in body size and addition of olfactory receptor neurons. The number of EcRlabelled cells was higher in premoult than in postmoult or intermoult in winter. The proliferation of nerve cells is regulated seasonally and according to moult stages. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011 2012-12-01T01:27:37Z 2012-12-01T01:27:37Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
HOLLMANN, Gabriela et al. Effects of seasonality and moult cycle on the proliferation of nerve cells and on the labelling of ecdysone receptors in an estuarine crab. Journal of comparative physiology, v. 197, p. 293-300, 2011. Disponível em: <http://download.springer.com/static/pdf/857/art%253A10.1007%252Fs00359-010-0611-8.pdf?auth66=1354061243_6efef69c861c60c3b23240976f332ce4&ext=.pdf>. Acesso em: 27 nov. 2012. 1432-1351 http://repositorio.furg.br/handle/1/2910 10.1007/s00359-010-0611-8 |
identifier_str_mv |
HOLLMANN, Gabriela et al. Effects of seasonality and moult cycle on the proliferation of nerve cells and on the labelling of ecdysone receptors in an estuarine crab. Journal of comparative physiology, v. 197, p. 293-300, 2011. Disponível em: <http://download.springer.com/static/pdf/857/art%253A10.1007%252Fs00359-010-0611-8.pdf?auth66=1354061243_6efef69c861c60c3b23240976f332ce4&ext=.pdf>. Acesso em: 27 nov. 2012. 1432-1351 10.1007/s00359-010-0611-8 |
url |
http://repositorio.furg.br/handle/1/2910 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da FURG (RI FURG) instname:Universidade Federal do Rio Grande (FURG) instacron:FURG |
instname_str |
Universidade Federal do Rio Grande (FURG) |
instacron_str |
FURG |
institution |
FURG |
reponame_str |
Repositório Institucional da FURG (RI FURG) |
collection |
Repositório Institucional da FURG (RI FURG) |
repository.name.fl_str_mv |
Repositório Institucional da FURG (RI FURG) - Universidade Federal do Rio Grande (FURG) |
repository.mail.fl_str_mv |
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1807384362497343488 |