Raw glycerol as substrate for the production of yeast biomass
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FURG (RI FURG) |
Texto Completo: | http://repositorio.furg.br/handle/1/4702 |
Resumo: | The possibility of using raw glycerol as a substrate for yeast biomass production as a source of proteins was investigated. Biomass concentration, protein content and total protein content of four yeasts (Yarrowia lipolytica NRRL YB-423, Candida lipolytica NRRL Y-1095, Candida utilis NRRL Y-900 and Candida rugosa NRRL Y-95) were determinate, comparing analytical grade glycerol and raw glycerol. Y. lipolytica NRRL YB-423 has been selected as promising for cultivation in a raw glycerol-based medium, mainly due to the higher biomass concentration in relation to the other strains. For this strain, four different culture media were tested. The best results were obtained with 50 g/L glycerol, 5.5 g/L ammonium phosphate, 5.5 potassium dihydrogen phosphate, 1 g/L ammonium sulphate, 0.25 g/L magnesium sulphate, 0.021 g/L calcium chloride dehydrate, 1 g/L yeast extract, 1 g/L peptone, pH adjusted to 5.5. In these conditions, it was possible to obtain 17.8 ± 0.6 g/L maximum biomass concentration, 18.2 ± 1.0% protein content and 3.1 ± 0.1 g/L total protein production. These results represent a 1.2-fold increase in biomass concentration, a 1.5-fold increase in protein content and a 1.9-fold increase in total protein production in relation to the results obtained with the previously medium composition. |
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Raw glycerol as substrate for the production of yeast biomassSingle cell proteinRaw glycerolBiodieselYeast biomassThe possibility of using raw glycerol as a substrate for yeast biomass production as a source of proteins was investigated. Biomass concentration, protein content and total protein content of four yeasts (Yarrowia lipolytica NRRL YB-423, Candida lipolytica NRRL Y-1095, Candida utilis NRRL Y-900 and Candida rugosa NRRL Y-95) were determinate, comparing analytical grade glycerol and raw glycerol. Y. lipolytica NRRL YB-423 has been selected as promising for cultivation in a raw glycerol-based medium, mainly due to the higher biomass concentration in relation to the other strains. For this strain, four different culture media were tested. The best results were obtained with 50 g/L glycerol, 5.5 g/L ammonium phosphate, 5.5 potassium dihydrogen phosphate, 1 g/L ammonium sulphate, 0.25 g/L magnesium sulphate, 0.021 g/L calcium chloride dehydrate, 1 g/L yeast extract, 1 g/L peptone, pH adjusted to 5.5. In these conditions, it was possible to obtain 17.8 ± 0.6 g/L maximum biomass concentration, 18.2 ± 1.0% protein content and 3.1 ± 0.1 g/L total protein production. These results represent a 1.2-fold increase in biomass concentration, a 1.5-fold increase in protein content and a 1.9-fold increase in total protein production in relation to the results obtained with the previously medium composition.De Gruyter2014-12-04T23:49:37Z2014-12-04T23:49:37Z2013info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfSANTOS, Elisane Odriosolla et al. Raw glycerol as substrate for the production of yeast biomass. International Journal of Food Engineering, v. 9, n. 4, p. 413-420, 2013. Disponível em: <http://www.degruyter.com/view/j/ijfe.2013.9.issue-4/ijfe-2012-0248/ijfe-2012-0248.xml>. Acesso em: 14 out. 2014.1556-3758http://repositorio.furg.br/handle/1/470210.1515/ijfe-2012-0248engSantos, Elisane Odriosolla dosMichelon, MarianoGallas, Julia AdornesKalil, Susana JulianoBurkert, Carlos André Veigainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FURG (RI FURG)instname:Universidade Federal do Rio Grande (FURG)instacron:FURG2019-12-04T01:19:35Zoai:repositorio.furg.br:1/4702Repositório InstitucionalPUBhttps://repositorio.furg.br/oai/request || http://200.19.254.174/oai/requestopendoar:2019-12-04T01:19:35Repositório Institucional da FURG (RI FURG) - Universidade Federal do Rio Grande (FURG)false |
dc.title.none.fl_str_mv |
Raw glycerol as substrate for the production of yeast biomass |
title |
Raw glycerol as substrate for the production of yeast biomass |
spellingShingle |
Raw glycerol as substrate for the production of yeast biomass Santos, Elisane Odriosolla dos Single cell protein Raw glycerol Biodiesel Yeast biomass |
title_short |
Raw glycerol as substrate for the production of yeast biomass |
title_full |
Raw glycerol as substrate for the production of yeast biomass |
title_fullStr |
Raw glycerol as substrate for the production of yeast biomass |
title_full_unstemmed |
Raw glycerol as substrate for the production of yeast biomass |
title_sort |
Raw glycerol as substrate for the production of yeast biomass |
author |
Santos, Elisane Odriosolla dos |
author_facet |
Santos, Elisane Odriosolla dos Michelon, Mariano Gallas, Julia Adornes Kalil, Susana Juliano Burkert, Carlos André Veiga |
author_role |
author |
author2 |
Michelon, Mariano Gallas, Julia Adornes Kalil, Susana Juliano Burkert, Carlos André Veiga |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Santos, Elisane Odriosolla dos Michelon, Mariano Gallas, Julia Adornes Kalil, Susana Juliano Burkert, Carlos André Veiga |
dc.subject.por.fl_str_mv |
Single cell protein Raw glycerol Biodiesel Yeast biomass |
topic |
Single cell protein Raw glycerol Biodiesel Yeast biomass |
description |
The possibility of using raw glycerol as a substrate for yeast biomass production as a source of proteins was investigated. Biomass concentration, protein content and total protein content of four yeasts (Yarrowia lipolytica NRRL YB-423, Candida lipolytica NRRL Y-1095, Candida utilis NRRL Y-900 and Candida rugosa NRRL Y-95) were determinate, comparing analytical grade glycerol and raw glycerol. Y. lipolytica NRRL YB-423 has been selected as promising for cultivation in a raw glycerol-based medium, mainly due to the higher biomass concentration in relation to the other strains. For this strain, four different culture media were tested. The best results were obtained with 50 g/L glycerol, 5.5 g/L ammonium phosphate, 5.5 potassium dihydrogen phosphate, 1 g/L ammonium sulphate, 0.25 g/L magnesium sulphate, 0.021 g/L calcium chloride dehydrate, 1 g/L yeast extract, 1 g/L peptone, pH adjusted to 5.5. In these conditions, it was possible to obtain 17.8 ± 0.6 g/L maximum biomass concentration, 18.2 ± 1.0% protein content and 3.1 ± 0.1 g/L total protein production. These results represent a 1.2-fold increase in biomass concentration, a 1.5-fold increase in protein content and a 1.9-fold increase in total protein production in relation to the results obtained with the previously medium composition. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013 2014-12-04T23:49:37Z 2014-12-04T23:49:37Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
SANTOS, Elisane Odriosolla et al. Raw glycerol as substrate for the production of yeast biomass. International Journal of Food Engineering, v. 9, n. 4, p. 413-420, 2013. Disponível em: <http://www.degruyter.com/view/j/ijfe.2013.9.issue-4/ijfe-2012-0248/ijfe-2012-0248.xml>. Acesso em: 14 out. 2014. 1556-3758 http://repositorio.furg.br/handle/1/4702 10.1515/ijfe-2012-0248 |
identifier_str_mv |
SANTOS, Elisane Odriosolla et al. Raw glycerol as substrate for the production of yeast biomass. International Journal of Food Engineering, v. 9, n. 4, p. 413-420, 2013. Disponível em: <http://www.degruyter.com/view/j/ijfe.2013.9.issue-4/ijfe-2012-0248/ijfe-2012-0248.xml>. Acesso em: 14 out. 2014. 1556-3758 10.1515/ijfe-2012-0248 |
url |
http://repositorio.furg.br/handle/1/4702 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
De Gruyter |
publisher.none.fl_str_mv |
De Gruyter |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da FURG (RI FURG) instname:Universidade Federal do Rio Grande (FURG) instacron:FURG |
instname_str |
Universidade Federal do Rio Grande (FURG) |
instacron_str |
FURG |
institution |
FURG |
reponame_str |
Repositório Institucional da FURG (RI FURG) |
collection |
Repositório Institucional da FURG (RI FURG) |
repository.name.fl_str_mv |
Repositório Institucional da FURG (RI FURG) - Universidade Federal do Rio Grande (FURG) |
repository.mail.fl_str_mv |
|
_version_ |
1807384369919164416 |