Antioxidant responses of laeonereis acuta (polychaeta) after exposure to hydrogen peroxide
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FURG (RI FURG) |
Texto Completo: | http://repositorio.furg.br/handle/1/4844 http://dx.doi.org/10.1590/S0100-879X2006005000201 |
Resumo: | The effects of H2O2 were evaluated in the estuarine worm Laeonereis acuta (Polychaeta, Nereididae) collected at the Patos Lagoon estuary (Southern Brazil) and maintained in the laboratory under controlled salinity (10 psu diluted seawater) and temperature (20°C). The worms were exposed to H2O2 (10 and 50 μM) for 4, 7, and 10 days and the following variables were determined: oxygen consumption, catalase (CAT) and glutathione peroxidase activity in both the supernatant and pellet fractions of whole body homogenates. The concentrations of non-protein sulfhydryl and lipid peroxides (LPO) were also measured. The oxygen consumption response was biphasic, decreasing after 4 days and increasing after 7 and 10 days of exposure to 50 μM H2O2 (P < 0.05). At the same H2O2 concentration, CAT activity was lower (P < 0.05) in the pellet fraction of worms exposed for 10 days compared to control. Non-protein sulfhydryl concentration and glutathione peroxidase activity were not affected by H2O2 exposure. After 10 days, LPO levels were higher (P < 0.05) in worms exposed to 50 μM H2O2 compared to control. The reduction in the antioxidant defense was paralleled by oxidative stress as indicated by higher LPO values (441% compared to control). The reduction of CAT activity in the pellet fraction may be related to protein oxidation. These results, taken together with previous findings, suggest that the worms were not able to cope with this H2O2 concentration. |
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Antioxidant responses of laeonereis acuta (polychaeta) after exposure to hydrogen peroxideWormsLaeonereis acutaHydrogen peroxideOxidative stressAntioxidant enzymesOxygen consumptionThe effects of H2O2 were evaluated in the estuarine worm Laeonereis acuta (Polychaeta, Nereididae) collected at the Patos Lagoon estuary (Southern Brazil) and maintained in the laboratory under controlled salinity (10 psu diluted seawater) and temperature (20°C). The worms were exposed to H2O2 (10 and 50 μM) for 4, 7, and 10 days and the following variables were determined: oxygen consumption, catalase (CAT) and glutathione peroxidase activity in both the supernatant and pellet fractions of whole body homogenates. The concentrations of non-protein sulfhydryl and lipid peroxides (LPO) were also measured. The oxygen consumption response was biphasic, decreasing after 4 days and increasing after 7 and 10 days of exposure to 50 μM H2O2 (P < 0.05). At the same H2O2 concentration, CAT activity was lower (P < 0.05) in the pellet fraction of worms exposed for 10 days compared to control. Non-protein sulfhydryl concentration and glutathione peroxidase activity were not affected by H2O2 exposure. After 10 days, LPO levels were higher (P < 0.05) in worms exposed to 50 μM H2O2 compared to control. The reduction in the antioxidant defense was paralleled by oxidative stress as indicated by higher LPO values (441% compared to control). The reduction of CAT activity in the pellet fraction may be related to protein oxidation. These results, taken together with previous findings, suggest that the worms were not able to cope with this H2O2 concentration.2015-05-14T20:52:15Z2015-05-14T20:52:15Z2008info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfROSA, C.E. da; BIANCHINI, A.; MONSERRAT, J.M.. Antioxidant responses of Laeonereis acuta (Polychaeta) after exposure to hydrogen peroxide. Brazilian Journal of Medical and Biological Research, v. 41, n. 2, p. 117-121, 2008. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000200007&lng=en&nrm=iso>. Acesso em: 05 maio de 2015.0100-879X1414-431Xhttp://repositorio.furg.br/handle/1/4844http://dx.doi.org/10.1590/S0100-879X2006005000201engRosa, Carlos Eduardo daBianchini, AdaltoMonserrat, José Maríainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FURG (RI FURG)instname:Universidade Federal do Rio Grande (FURG)instacron:FURG2015-05-14T20:52:15Zoai:repositorio.furg.br:1/4844Repositório InstitucionalPUBhttps://repositorio.furg.br/oai/request || http://200.19.254.174/oai/requestopendoar:2015-05-14T20:52:15Repositório Institucional da FURG (RI FURG) - Universidade Federal do Rio Grande (FURG)false |
dc.title.none.fl_str_mv |
Antioxidant responses of laeonereis acuta (polychaeta) after exposure to hydrogen peroxide |
title |
Antioxidant responses of laeonereis acuta (polychaeta) after exposure to hydrogen peroxide |
spellingShingle |
Antioxidant responses of laeonereis acuta (polychaeta) after exposure to hydrogen peroxide Rosa, Carlos Eduardo da Worms Laeonereis acuta Hydrogen peroxide Oxidative stress Antioxidant enzymes Oxygen consumption |
title_short |
Antioxidant responses of laeonereis acuta (polychaeta) after exposure to hydrogen peroxide |
title_full |
Antioxidant responses of laeonereis acuta (polychaeta) after exposure to hydrogen peroxide |
title_fullStr |
Antioxidant responses of laeonereis acuta (polychaeta) after exposure to hydrogen peroxide |
title_full_unstemmed |
Antioxidant responses of laeonereis acuta (polychaeta) after exposure to hydrogen peroxide |
title_sort |
Antioxidant responses of laeonereis acuta (polychaeta) after exposure to hydrogen peroxide |
author |
Rosa, Carlos Eduardo da |
author_facet |
Rosa, Carlos Eduardo da Bianchini, Adalto Monserrat, José María |
author_role |
author |
author2 |
Bianchini, Adalto Monserrat, José María |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Rosa, Carlos Eduardo da Bianchini, Adalto Monserrat, José María |
dc.subject.por.fl_str_mv |
Worms Laeonereis acuta Hydrogen peroxide Oxidative stress Antioxidant enzymes Oxygen consumption |
topic |
Worms Laeonereis acuta Hydrogen peroxide Oxidative stress Antioxidant enzymes Oxygen consumption |
description |
The effects of H2O2 were evaluated in the estuarine worm Laeonereis acuta (Polychaeta, Nereididae) collected at the Patos Lagoon estuary (Southern Brazil) and maintained in the laboratory under controlled salinity (10 psu diluted seawater) and temperature (20°C). The worms were exposed to H2O2 (10 and 50 μM) for 4, 7, and 10 days and the following variables were determined: oxygen consumption, catalase (CAT) and glutathione peroxidase activity in both the supernatant and pellet fractions of whole body homogenates. The concentrations of non-protein sulfhydryl and lipid peroxides (LPO) were also measured. The oxygen consumption response was biphasic, decreasing after 4 days and increasing after 7 and 10 days of exposure to 50 μM H2O2 (P < 0.05). At the same H2O2 concentration, CAT activity was lower (P < 0.05) in the pellet fraction of worms exposed for 10 days compared to control. Non-protein sulfhydryl concentration and glutathione peroxidase activity were not affected by H2O2 exposure. After 10 days, LPO levels were higher (P < 0.05) in worms exposed to 50 μM H2O2 compared to control. The reduction in the antioxidant defense was paralleled by oxidative stress as indicated by higher LPO values (441% compared to control). The reduction of CAT activity in the pellet fraction may be related to protein oxidation. These results, taken together with previous findings, suggest that the worms were not able to cope with this H2O2 concentration. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008 2015-05-14T20:52:15Z 2015-05-14T20:52:15Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
ROSA, C.E. da; BIANCHINI, A.; MONSERRAT, J.M.. Antioxidant responses of Laeonereis acuta (Polychaeta) after exposure to hydrogen peroxide. Brazilian Journal of Medical and Biological Research, v. 41, n. 2, p. 117-121, 2008. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000200007&lng=en&nrm=iso>. Acesso em: 05 maio de 2015. 0100-879X 1414-431X http://repositorio.furg.br/handle/1/4844 http://dx.doi.org/10.1590/S0100-879X2006005000201 |
identifier_str_mv |
ROSA, C.E. da; BIANCHINI, A.; MONSERRAT, J.M.. Antioxidant responses of Laeonereis acuta (Polychaeta) after exposure to hydrogen peroxide. Brazilian Journal of Medical and Biological Research, v. 41, n. 2, p. 117-121, 2008. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000200007&lng=en&nrm=iso>. Acesso em: 05 maio de 2015. 0100-879X 1414-431X |
url |
http://repositorio.furg.br/handle/1/4844 http://dx.doi.org/10.1590/S0100-879X2006005000201 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da FURG (RI FURG) instname:Universidade Federal do Rio Grande (FURG) instacron:FURG |
instname_str |
Universidade Federal do Rio Grande (FURG) |
instacron_str |
FURG |
institution |
FURG |
reponame_str |
Repositório Institucional da FURG (RI FURG) |
collection |
Repositório Institucional da FURG (RI FURG) |
repository.name.fl_str_mv |
Repositório Institucional da FURG (RI FURG) - Universidade Federal do Rio Grande (FURG) |
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