Relative increase of deoxy sugars during microbial degradation of an extracellular polysaccharide released by a tropical freshwater thalassiosira sp, (bacillariophyceae)
Autor(a) principal: | |
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Data de Publicação: | 2003 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FURG (RI FURG) |
Texto Completo: | http://repositorio.furg.br/handle/1/2834 |
Resumo: | The aim of this study was to characterize the extracellular polysaccharides (EPS) released by a freshwater Thalassiosira sp. (Bacillariophyceae) and evaluate their degradation by heterotrophic microbial populations from the same habitat of Thalassiosira sp., a tropical eutrophic reservoir. The EPS were purified by anion exchange column chromatography, the monosaccharide composition was determined by GC, and the linkages of the monosaccharides by GC-MS. The EPS is a mannose-rich heteropolysaccharide composed of two different acidic fractions. Both of these fractions are composed of mannose, rhamnose, fucose, xylose, galactose, glucose, glucuronic acid, and N-acetyl glucosamine but with different proportions. N-acetyl galactosamine occurs only in fraction 1 and galacturonic acid only in fraction 2. We monitored the concentrations of the monosaccharides in the EPS during its degradation using pulse amperometric detection in an HPLC. The decay patterns of the monosaccharides were varied and the deoxy sugars, fucose and rhamnose, were degraded at a slower rate than the other components, increasing their relative concentrations and the hydrophobic feature of the EPS. The possibility of a selective degradation, which enhances the stickiness of the EPS, promoting transparent exopolymeric particles and aggregate formation, is discussed based on the literature data. |
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Relative increase of deoxy sugars during microbial degradation of an extracellular polysaccharide released by a tropical freshwater thalassiosira sp, (bacillariophyceae)Carbohydrate releaseDiatomEPSMicrobial degradationThalassiosiraThe aim of this study was to characterize the extracellular polysaccharides (EPS) released by a freshwater Thalassiosira sp. (Bacillariophyceae) and evaluate their degradation by heterotrophic microbial populations from the same habitat of Thalassiosira sp., a tropical eutrophic reservoir. The EPS were purified by anion exchange column chromatography, the monosaccharide composition was determined by GC, and the linkages of the monosaccharides by GC-MS. The EPS is a mannose-rich heteropolysaccharide composed of two different acidic fractions. Both of these fractions are composed of mannose, rhamnose, fucose, xylose, galactose, glucose, glucuronic acid, and N-acetyl glucosamine but with different proportions. N-acetyl galactosamine occurs only in fraction 1 and galacturonic acid only in fraction 2. We monitored the concentrations of the monosaccharides in the EPS during its degradation using pulse amperometric detection in an HPLC. The decay patterns of the monosaccharides were varied and the deoxy sugars, fucose and rhamnose, were degraded at a slower rate than the other components, increasing their relative concentrations and the hydrophobic feature of the EPS. The possibility of a selective degradation, which enhances the stickiness of the EPS, promoting transparent exopolymeric particles and aggregate formation, is discussed based on the literature data.2012-11-27T20:14:15Z2012-11-27T20:14:15Z2003info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfGIROLDO, Danilo; VIEIRA, Armando Augusto Henriques; PAULSEN, Berit Smestad. Relative increase of deoxy sugars during microbial degradation of an extracellular polysaccharide released by a tropical freshwater thalassiosira sp, (bacillariophyceae). Journal of Phycology, v. 39, p. 1109-1115, 2003. Disponível em:<http://content.ebscohost.com/pdf9/pdf/2003/FR6/01Dec03/11522640.pdf?T=P&P=AN&K=11522640&S=R&D=aph&EbscoContent=dGJyMNLr40SeqLE4zdnyOLCmr0qeprZSr6q4TLSWxWXS&ContentCustomer=dGJyMPGssk2xqLJNuePfgeyx44Hy>. Acesso em: 22 nov. 2012.http://repositorio.furg.br/handle/1/2834engGiroldo, DaniloVieira, Armando Augusto HenriquesPaulsen, Berit Smestadinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FURG (RI FURG)instname:Universidade Federal do Rio Grande (FURG)instacron:FURG2012-11-27T20:14:15Zoai:repositorio.furg.br:1/2834Repositório InstitucionalPUBhttps://repositorio.furg.br/oai/request || http://200.19.254.174/oai/requestopendoar:2012-11-27T20:14:15Repositório Institucional da FURG (RI FURG) - Universidade Federal do Rio Grande (FURG)false |
dc.title.none.fl_str_mv |
Relative increase of deoxy sugars during microbial degradation of an extracellular polysaccharide released by a tropical freshwater thalassiosira sp, (bacillariophyceae) |
title |
Relative increase of deoxy sugars during microbial degradation of an extracellular polysaccharide released by a tropical freshwater thalassiosira sp, (bacillariophyceae) |
spellingShingle |
Relative increase of deoxy sugars during microbial degradation of an extracellular polysaccharide released by a tropical freshwater thalassiosira sp, (bacillariophyceae) Giroldo, Danilo Carbohydrate release Diatom EPS Microbial degradation Thalassiosira |
title_short |
Relative increase of deoxy sugars during microbial degradation of an extracellular polysaccharide released by a tropical freshwater thalassiosira sp, (bacillariophyceae) |
title_full |
Relative increase of deoxy sugars during microbial degradation of an extracellular polysaccharide released by a tropical freshwater thalassiosira sp, (bacillariophyceae) |
title_fullStr |
Relative increase of deoxy sugars during microbial degradation of an extracellular polysaccharide released by a tropical freshwater thalassiosira sp, (bacillariophyceae) |
title_full_unstemmed |
Relative increase of deoxy sugars during microbial degradation of an extracellular polysaccharide released by a tropical freshwater thalassiosira sp, (bacillariophyceae) |
title_sort |
Relative increase of deoxy sugars during microbial degradation of an extracellular polysaccharide released by a tropical freshwater thalassiosira sp, (bacillariophyceae) |
author |
Giroldo, Danilo |
author_facet |
Giroldo, Danilo Vieira, Armando Augusto Henriques Paulsen, Berit Smestad |
author_role |
author |
author2 |
Vieira, Armando Augusto Henriques Paulsen, Berit Smestad |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Giroldo, Danilo Vieira, Armando Augusto Henriques Paulsen, Berit Smestad |
dc.subject.por.fl_str_mv |
Carbohydrate release Diatom EPS Microbial degradation Thalassiosira |
topic |
Carbohydrate release Diatom EPS Microbial degradation Thalassiosira |
description |
The aim of this study was to characterize the extracellular polysaccharides (EPS) released by a freshwater Thalassiosira sp. (Bacillariophyceae) and evaluate their degradation by heterotrophic microbial populations from the same habitat of Thalassiosira sp., a tropical eutrophic reservoir. The EPS were purified by anion exchange column chromatography, the monosaccharide composition was determined by GC, and the linkages of the monosaccharides by GC-MS. The EPS is a mannose-rich heteropolysaccharide composed of two different acidic fractions. Both of these fractions are composed of mannose, rhamnose, fucose, xylose, galactose, glucose, glucuronic acid, and N-acetyl glucosamine but with different proportions. N-acetyl galactosamine occurs only in fraction 1 and galacturonic acid only in fraction 2. We monitored the concentrations of the monosaccharides in the EPS during its degradation using pulse amperometric detection in an HPLC. The decay patterns of the monosaccharides were varied and the deoxy sugars, fucose and rhamnose, were degraded at a slower rate than the other components, increasing their relative concentrations and the hydrophobic feature of the EPS. The possibility of a selective degradation, which enhances the stickiness of the EPS, promoting transparent exopolymeric particles and aggregate formation, is discussed based on the literature data. |
publishDate |
2003 |
dc.date.none.fl_str_mv |
2003 2012-11-27T20:14:15Z 2012-11-27T20:14:15Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
GIROLDO, Danilo; VIEIRA, Armando Augusto Henriques; PAULSEN, Berit Smestad. Relative increase of deoxy sugars during microbial degradation of an extracellular polysaccharide released by a tropical freshwater thalassiosira sp, (bacillariophyceae). Journal of Phycology, v. 39, p. 1109-1115, 2003. Disponível em:<http://content.ebscohost.com/pdf9/pdf/2003/FR6/01Dec03/11522640.pdf?T=P&P=AN&K=11522640&S=R&D=aph&EbscoContent=dGJyMNLr40SeqLE4zdnyOLCmr0qeprZSr6q4TLSWxWXS&ContentCustomer=dGJyMPGssk2xqLJNuePfgeyx44Hy>. Acesso em: 22 nov. 2012. http://repositorio.furg.br/handle/1/2834 |
identifier_str_mv |
GIROLDO, Danilo; VIEIRA, Armando Augusto Henriques; PAULSEN, Berit Smestad. Relative increase of deoxy sugars during microbial degradation of an extracellular polysaccharide released by a tropical freshwater thalassiosira sp, (bacillariophyceae). Journal of Phycology, v. 39, p. 1109-1115, 2003. Disponível em:<http://content.ebscohost.com/pdf9/pdf/2003/FR6/01Dec03/11522640.pdf?T=P&P=AN&K=11522640&S=R&D=aph&EbscoContent=dGJyMNLr40SeqLE4zdnyOLCmr0qeprZSr6q4TLSWxWXS&ContentCustomer=dGJyMPGssk2xqLJNuePfgeyx44Hy>. Acesso em: 22 nov. 2012. |
url |
http://repositorio.furg.br/handle/1/2834 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da FURG (RI FURG) instname:Universidade Federal do Rio Grande (FURG) instacron:FURG |
instname_str |
Universidade Federal do Rio Grande (FURG) |
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FURG |
institution |
FURG |
reponame_str |
Repositório Institucional da FURG (RI FURG) |
collection |
Repositório Institucional da FURG (RI FURG) |
repository.name.fl_str_mv |
Repositório Institucional da FURG (RI FURG) - Universidade Federal do Rio Grande (FURG) |
repository.mail.fl_str_mv |
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1807384403751469056 |