A comparative expression analysis of gene transcripts in brain tissue of non-transgenic and GH-transgenic zebrafish (Danio rerio) using a DDRT-PCR approach

Detalhes bibliográficos
Autor(a) principal: Alves-Costa, Fernanda Antunes
Data de Publicação: 2012
Outros Autores: Figueiredo, Marcio de Azevedo, Lanes, Carlos Frederico Ceccon, Almeida, Daniela Volcan, Marins, Luis Fernando Fernandes, Wasko, Adriane Pinto
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FURG (RI FURG)
Texto Completo: http://repositorio.furg.br/handle/1/3198
http://dx.doi.org/10.1590/S0001-37652012005000033
Resumo: The presence of higher level of exogenous growth hormone (GH) in transgenic animals could lead to several physiological alterations. A GH transgenic zebrafish (Danio rerio) line was compared to nontransgenic (NT) samples of the species through a DDRT-PCR approach, with the goal of identifying candidate differentially expressed transcripts in brain tissues that could be involved in GH overexpression. Densitometric analyses of two selected amplification products, p300 and ADCY2, pointed to a significant lower gene expression in the transgenic zebrafish (104.02 ± 57.71; 224.10 ± 91.73) when compared to NT samples (249.75 ± 30.08; 342.95 ± 65.19). The present data indicate that p300 and ADCY2 are involved in a regulation system for GH when high circulating levels of this hormone are found in zebrafishes.
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spelling A comparative expression analysis of gene transcripts in brain tissue of non-transgenic and GH-transgenic zebrafish (Danio rerio) using a DDRT-PCR approachDifferential displayGHGrowth hormoneTransgenicZebrafishDisplay diferencialHormônio de crescimentoTransgênicoPaulistinhaThe presence of higher level of exogenous growth hormone (GH) in transgenic animals could lead to several physiological alterations. A GH transgenic zebrafish (Danio rerio) line was compared to nontransgenic (NT) samples of the species through a DDRT-PCR approach, with the goal of identifying candidate differentially expressed transcripts in brain tissues that could be involved in GH overexpression. Densitometric analyses of two selected amplification products, p300 and ADCY2, pointed to a significant lower gene expression in the transgenic zebrafish (104.02 ± 57.71; 224.10 ± 91.73) when compared to NT samples (249.75 ± 30.08; 342.95 ± 65.19). The present data indicate that p300 and ADCY2 are involved in a regulation system for GH when high circulating levels of this hormone are found in zebrafishes.A presença de níveis mais elevados do hormônio de crescimento (GH) em animais transgênicos poderia levar a várias alterações fisiológicas. Uma linhagem transgênica de paulistinha (Danio rerio) para o GH foi comparada com amostras não transgênicas (NT) desta espécie, através de uma abordagem de DDRT-PCR, com o objetivo de identificar transcritos candidatos diferencialmente expressos em tecido cerebral que poderiam estar envolvidos na superexpressão de GH. Análises densitométricas de dois produtos de amplificação selecionados, p300 e ADCY2, apontaram uma expressão gênica significativamente menor nas amostras transgênicas de paulistinha (104.02 ± 57.71; 224.10 ± 91.73), quando comparadas com as amostras NT (249.75 ± 30.08; 342.95±65.19). Os presentes dados indicam que p300 e ADCY2 estão envolvidos em um sistema de regulação do GH, quando altos níveis circulantes desse hormônio são encontrados em paulistinha.2013-03-24T23:29:05Z2013-03-24T23:29:05Z2012info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfALVES-COSTA, Fernanda Antunes et al. A comparative expression analysis of gene transcripts in brain tissue of non-transgenic and GH-transgenic zebrafish (Danio rerio) using a DDRT-PCR approach. Anais da Academia Brasileira de Ciências, v. 84, n. 2, p. 487-494, 2012. Disponível em: <http://www.scielo.br/scielo.php?pid=S0001-37652012000200017&script=sci_arttext>. Acesso em: 10 nov. 2012.0001-3765http://repositorio.furg.br/handle/1/3198http://dx.doi.org/10.1590/S0001-37652012005000033engAlves-Costa, Fernanda AntunesFigueiredo, Marcio de AzevedoLanes, Carlos Frederico CecconAlmeida, Daniela VolcanMarins, Luis Fernando FernandesWasko, Adriane Pintoinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FURG (RI FURG)instname:Universidade Federal do Rio Grande (FURG)instacron:FURG2019-11-11T19:17:41Zoai:repositorio.furg.br:1/3198Repositório InstitucionalPUBhttps://repositorio.furg.br/oai/request || http://200.19.254.174/oai/requestopendoar:2019-11-11T19:17:41Repositório Institucional da FURG (RI FURG) - Universidade Federal do Rio Grande (FURG)false
dc.title.none.fl_str_mv A comparative expression analysis of gene transcripts in brain tissue of non-transgenic and GH-transgenic zebrafish (Danio rerio) using a DDRT-PCR approach
title A comparative expression analysis of gene transcripts in brain tissue of non-transgenic and GH-transgenic zebrafish (Danio rerio) using a DDRT-PCR approach
spellingShingle A comparative expression analysis of gene transcripts in brain tissue of non-transgenic and GH-transgenic zebrafish (Danio rerio) using a DDRT-PCR approach
Alves-Costa, Fernanda Antunes
Differential display
GH
Growth hormone
Transgenic
Zebrafish
Display diferencial
Hormônio de crescimento
Transgênico
Paulistinha
title_short A comparative expression analysis of gene transcripts in brain tissue of non-transgenic and GH-transgenic zebrafish (Danio rerio) using a DDRT-PCR approach
title_full A comparative expression analysis of gene transcripts in brain tissue of non-transgenic and GH-transgenic zebrafish (Danio rerio) using a DDRT-PCR approach
title_fullStr A comparative expression analysis of gene transcripts in brain tissue of non-transgenic and GH-transgenic zebrafish (Danio rerio) using a DDRT-PCR approach
title_full_unstemmed A comparative expression analysis of gene transcripts in brain tissue of non-transgenic and GH-transgenic zebrafish (Danio rerio) using a DDRT-PCR approach
title_sort A comparative expression analysis of gene transcripts in brain tissue of non-transgenic and GH-transgenic zebrafish (Danio rerio) using a DDRT-PCR approach
author Alves-Costa, Fernanda Antunes
author_facet Alves-Costa, Fernanda Antunes
Figueiredo, Marcio de Azevedo
Lanes, Carlos Frederico Ceccon
Almeida, Daniela Volcan
Marins, Luis Fernando Fernandes
Wasko, Adriane Pinto
author_role author
author2 Figueiredo, Marcio de Azevedo
Lanes, Carlos Frederico Ceccon
Almeida, Daniela Volcan
Marins, Luis Fernando Fernandes
Wasko, Adriane Pinto
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Alves-Costa, Fernanda Antunes
Figueiredo, Marcio de Azevedo
Lanes, Carlos Frederico Ceccon
Almeida, Daniela Volcan
Marins, Luis Fernando Fernandes
Wasko, Adriane Pinto
dc.subject.por.fl_str_mv Differential display
GH
Growth hormone
Transgenic
Zebrafish
Display diferencial
Hormônio de crescimento
Transgênico
Paulistinha
topic Differential display
GH
Growth hormone
Transgenic
Zebrafish
Display diferencial
Hormônio de crescimento
Transgênico
Paulistinha
description The presence of higher level of exogenous growth hormone (GH) in transgenic animals could lead to several physiological alterations. A GH transgenic zebrafish (Danio rerio) line was compared to nontransgenic (NT) samples of the species through a DDRT-PCR approach, with the goal of identifying candidate differentially expressed transcripts in brain tissues that could be involved in GH overexpression. Densitometric analyses of two selected amplification products, p300 and ADCY2, pointed to a significant lower gene expression in the transgenic zebrafish (104.02 ± 57.71; 224.10 ± 91.73) when compared to NT samples (249.75 ± 30.08; 342.95 ± 65.19). The present data indicate that p300 and ADCY2 are involved in a regulation system for GH when high circulating levels of this hormone are found in zebrafishes.
publishDate 2012
dc.date.none.fl_str_mv 2012
2013-03-24T23:29:05Z
2013-03-24T23:29:05Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv ALVES-COSTA, Fernanda Antunes et al. A comparative expression analysis of gene transcripts in brain tissue of non-transgenic and GH-transgenic zebrafish (Danio rerio) using a DDRT-PCR approach. Anais da Academia Brasileira de Ciências, v. 84, n. 2, p. 487-494, 2012. Disponível em: <http://www.scielo.br/scielo.php?pid=S0001-37652012000200017&script=sci_arttext>. Acesso em: 10 nov. 2012.
0001-3765
http://repositorio.furg.br/handle/1/3198
http://dx.doi.org/10.1590/S0001-37652012005000033
identifier_str_mv ALVES-COSTA, Fernanda Antunes et al. A comparative expression analysis of gene transcripts in brain tissue of non-transgenic and GH-transgenic zebrafish (Danio rerio) using a DDRT-PCR approach. Anais da Academia Brasileira de Ciências, v. 84, n. 2, p. 487-494, 2012. Disponível em: <http://www.scielo.br/scielo.php?pid=S0001-37652012000200017&script=sci_arttext>. Acesso em: 10 nov. 2012.
0001-3765
url http://repositorio.furg.br/handle/1/3198
http://dx.doi.org/10.1590/S0001-37652012005000033
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Institucional da FURG (RI FURG)
instname:Universidade Federal do Rio Grande (FURG)
instacron:FURG
instname_str Universidade Federal do Rio Grande (FURG)
instacron_str FURG
institution FURG
reponame_str Repositório Institucional da FURG (RI FURG)
collection Repositório Institucional da FURG (RI FURG)
repository.name.fl_str_mv Repositório Institucional da FURG (RI FURG) - Universidade Federal do Rio Grande (FURG)
repository.mail.fl_str_mv
_version_ 1813187249884364800

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