Ondas ultrassônicas e pérolas de vidro: um novo método de extração de b-galactosidase para uso em laboratório

Detalhes bibliográficos
Autor(a) principal: Medeiros, Fabiana Oliveira de
Data de Publicação: 2008
Outros Autores: Alves, Fernanda Germano, Lisboa, Cristiane Reinaldo, Martins, Daniela de Souza, Burkert, Carlos André Veiga, Kalil, Susana Juliano
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FURG (RI FURG)
Texto Completo: http://repositorio.furg.br/handle/1/4649
Resumo: The optimization of a traditional technique of cellular disruption by abrasion was carried out and a process using ultrasonic waves associated with glass pearls to extract β-galactosidase from Kluyveromyces marxianus proposed. In the first case, the effects of the diameter and weight of the pearls in relation to the volume of cellular suspension and amount of time for cellular disruption were evaluated. The efficiency of the new process of cellular disruption was evaluated by varying the length of time of sonification and comparing with the method of abrasion under the same conditions. The proposed method can be efficiently applied to obtain β-galactosidase at laboratory scale.
id FURG_9412a545d1aac44e8438281688ad6f8f
oai_identifier_str oai:repositorio.furg.br:1/4649
network_acronym_str FURG
network_name_str Repositório Institucional da FURG (RI FURG)
repository_id_str
spelling Medeiros, Fabiana Oliveira deAlves, Fernanda GermanoLisboa, Cristiane ReinaldoMartins, Daniela de SouzaBurkert, Carlos André VeigaKalil, Susana Juliano2014-10-25T18:50:57Z2014-10-25T18:50:57Z2008MEDEIROS, Fabiana Oliveira de et al. Ondas ultrassônicas e pérolas de vidro: um novo método de extração de b-galactosidase para uso em laboratório. Química Nova, v. 31, n. 2, 336-339, 2008. Disponível em: <www.scielo.br/pdf/qn/v31n2/a28v31n2>. Acesso em: 09 set. 20141678-7064http://repositorio.furg.br/handle/1/4649The optimization of a traditional technique of cellular disruption by abrasion was carried out and a process using ultrasonic waves associated with glass pearls to extract β-galactosidase from Kluyveromyces marxianus proposed. In the first case, the effects of the diameter and weight of the pearls in relation to the volume of cellular suspension and amount of time for cellular disruption were evaluated. The efficiency of the new process of cellular disruption was evaluated by varying the length of time of sonification and comparing with the method of abrasion under the same conditions. The proposed method can be efficiently applied to obtain β-galactosidase at laboratory scale.porB-galactosidaseCellular disruptionSonificationOndas ultrassônicas e pérolas de vidro: um novo método de extração de b-galactosidase para uso em laboratórioUltrasonic waves and glass pearls: a new method of extraction of β-galactosidase for use in laboratoryinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FURG (RI FURG)instname:Universidade Federal do Rio Grande (FURG)instacron:FURGORIGINAL43 - ONDAS ULTRASSÔNICAS E PÉROLAS DE VIDRO UM NOVO MÉTODO DE EXTRAÇÃO DE b-GALACTOSIDASE.pdf43 - ONDAS ULTRASSÔNICAS E PÉROLAS DE VIDRO UM NOVO MÉTODO DE EXTRAÇÃO DE b-GALACTOSIDASE.pdfapplication/pdf97559https://repositorio.furg.br/bitstream/1/4649/1/43%20-%20ONDAS%20ULTRASS%c3%94NICAS%20E%20P%c3%89ROLAS%20DE%20VIDRO%20UM%20NOVO%20M%c3%89TODO%20DE%20EXTRA%c3%87%c3%83O%20DE%20b-GALACTOSIDASE.pdf1674b8edc2a02c507bd67458eafd14c2MD51open accessLICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://repositorio.furg.br/bitstream/1/4649/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52open access1/46492014-10-25 16:50:57.024open accessoai:repositorio.furg.br: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Repositório InstitucionalPUBhttps://repositorio.furg.br/oai/request || http://200.19.254.174/oai/requestopendoar:2014-10-25T18:50:57Repositório Institucional da FURG (RI FURG) - Universidade Federal do Rio Grande (FURG)false
dc.title.pt_BR.fl_str_mv Ondas ultrassônicas e pérolas de vidro: um novo método de extração de b-galactosidase para uso em laboratório
dc.title.alternative.pt_BR.fl_str_mv Ultrasonic waves and glass pearls: a new method of extraction of β-galactosidase for use in laboratory
title Ondas ultrassônicas e pérolas de vidro: um novo método de extração de b-galactosidase para uso em laboratório
spellingShingle Ondas ultrassônicas e pérolas de vidro: um novo método de extração de b-galactosidase para uso em laboratório
Medeiros, Fabiana Oliveira de
B-galactosidase
Cellular disruption
Sonification
title_short Ondas ultrassônicas e pérolas de vidro: um novo método de extração de b-galactosidase para uso em laboratório
title_full Ondas ultrassônicas e pérolas de vidro: um novo método de extração de b-galactosidase para uso em laboratório
title_fullStr Ondas ultrassônicas e pérolas de vidro: um novo método de extração de b-galactosidase para uso em laboratório
title_full_unstemmed Ondas ultrassônicas e pérolas de vidro: um novo método de extração de b-galactosidase para uso em laboratório
title_sort Ondas ultrassônicas e pérolas de vidro: um novo método de extração de b-galactosidase para uso em laboratório
author Medeiros, Fabiana Oliveira de
author_facet Medeiros, Fabiana Oliveira de
Alves, Fernanda Germano
Lisboa, Cristiane Reinaldo
Martins, Daniela de Souza
Burkert, Carlos André Veiga
Kalil, Susana Juliano
author_role author
author2 Alves, Fernanda Germano
Lisboa, Cristiane Reinaldo
Martins, Daniela de Souza
Burkert, Carlos André Veiga
Kalil, Susana Juliano
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Medeiros, Fabiana Oliveira de
Alves, Fernanda Germano
Lisboa, Cristiane Reinaldo
Martins, Daniela de Souza
Burkert, Carlos André Veiga
Kalil, Susana Juliano
dc.subject.por.fl_str_mv B-galactosidase
Cellular disruption
Sonification
topic B-galactosidase
Cellular disruption
Sonification
description The optimization of a traditional technique of cellular disruption by abrasion was carried out and a process using ultrasonic waves associated with glass pearls to extract β-galactosidase from Kluyveromyces marxianus proposed. In the first case, the effects of the diameter and weight of the pearls in relation to the volume of cellular suspension and amount of time for cellular disruption were evaluated. The efficiency of the new process of cellular disruption was evaluated by varying the length of time of sonification and comparing with the method of abrasion under the same conditions. The proposed method can be efficiently applied to obtain β-galactosidase at laboratory scale.
publishDate 2008
dc.date.issued.fl_str_mv 2008
dc.date.accessioned.fl_str_mv 2014-10-25T18:50:57Z
dc.date.available.fl_str_mv 2014-10-25T18:50:57Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MEDEIROS, Fabiana Oliveira de et al. Ondas ultrassônicas e pérolas de vidro: um novo método de extração de b-galactosidase para uso em laboratório. Química Nova, v. 31, n. 2, 336-339, 2008. Disponível em: <www.scielo.br/pdf/qn/v31n2/a28v31n2>. Acesso em: 09 set. 2014
dc.identifier.uri.fl_str_mv http://repositorio.furg.br/handle/1/4649
dc.identifier.issn.none.fl_str_mv 1678-7064
identifier_str_mv MEDEIROS, Fabiana Oliveira de et al. Ondas ultrassônicas e pérolas de vidro: um novo método de extração de b-galactosidase para uso em laboratório. Química Nova, v. 31, n. 2, 336-339, 2008. Disponível em: <www.scielo.br/pdf/qn/v31n2/a28v31n2>. Acesso em: 09 set. 2014
1678-7064
url http://repositorio.furg.br/handle/1/4649
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da FURG (RI FURG)
instname:Universidade Federal do Rio Grande (FURG)
instacron:FURG
instname_str Universidade Federal do Rio Grande (FURG)
instacron_str FURG
institution FURG
reponame_str Repositório Institucional da FURG (RI FURG)
collection Repositório Institucional da FURG (RI FURG)
bitstream.url.fl_str_mv https://repositorio.furg.br/bitstream/1/4649/1/43%20-%20ONDAS%20ULTRASS%c3%94NICAS%20E%20P%c3%89ROLAS%20DE%20VIDRO%20UM%20NOVO%20M%c3%89TODO%20DE%20EXTRA%c3%87%c3%83O%20DE%20b-GALACTOSIDASE.pdf
https://repositorio.furg.br/bitstream/1/4649/2/license.txt
bitstream.checksum.fl_str_mv 1674b8edc2a02c507bd67458eafd14c2
8a4605be74aa9ea9d79846c1fba20a33
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
repository.name.fl_str_mv Repositório Institucional da FURG (RI FURG) - Universidade Federal do Rio Grande (FURG)
repository.mail.fl_str_mv
_version_ 1798313614755495936

Registros relacionados