The multidrug transporters belonging to major facilitator superfamily (MFS) in mycobacterium tuberculosis
Autor(a) principal: | |
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Data de Publicação: | 2002 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FURG (RI FURG) |
Texto Completo: | http://repositorio.furg.br/handle/1/3103 |
Resumo: | Background: Both intrinsic and acquired multidrug resistance play an important role in the insurgence of tuberculosis. Detailed knowledge of the molecular basis of drug recognition and transport by multidrug transport systems is required for the development of new antibiotics that are not extruded or of inhibitors that block the multidrug transporter and allow traditional antibiotics to be effective. Materials and Methods: We have undertaken the inventory of the drug transporters subfamily, included in the major facilitator superfamily (MFS), encoded by the complete genome of Mycobacterium tuberculosis (MTB). These proteins were identified on the basis of their characteristic stretches of amino acids and transmembrane segments (TMS) number. Conclusions: Genome analysis and searches of homology between the identified transporters and proteins characterized in other organisms revealed 16 open reading frames encoding putative drug efflux pumps belonging to MFS. In the case of two of them, we also have demonstrated that they function as drug efflux proteins. |
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Rossi, Edda deArrigo, PatrizioBellinzoni, MarcoSilva, Pedro Eduardo Almeida daMartín, CarlosAínsa, JoséGuglierame, PaolaRiccardi, Giovanna2013-01-17T20:10:44Z2013-01-17T20:10:44Z2002ROSSI, Edda de et al. The multidrug transporters belonging to major facilitator superfamily (MFS) in mycobacterium tuberculosis. Molecular Medicine, v. 8, n.11, p. 714-724, 2002. Disponível em: <http://130.203.133.150/viewdoc/summary;jsessionid=13E00EC2F38B4742D96AE349BE46AF22?doi=10.1.1.222.9957>. Acesso em: 18 set. 2012.http://repositorio.furg.br/handle/1/3103Background: Both intrinsic and acquired multidrug resistance play an important role in the insurgence of tuberculosis. Detailed knowledge of the molecular basis of drug recognition and transport by multidrug transport systems is required for the development of new antibiotics that are not extruded or of inhibitors that block the multidrug transporter and allow traditional antibiotics to be effective. Materials and Methods: We have undertaken the inventory of the drug transporters subfamily, included in the major facilitator superfamily (MFS), encoded by the complete genome of Mycobacterium tuberculosis (MTB). These proteins were identified on the basis of their characteristic stretches of amino acids and transmembrane segments (TMS) number. Conclusions: Genome analysis and searches of homology between the identified transporters and proteins characterized in other organisms revealed 16 open reading frames encoding putative drug efflux pumps belonging to MFS. In the case of two of them, we also have demonstrated that they function as drug efflux proteins.engThe multidrug transporters belonging to major facilitator superfamily (MFS) in mycobacterium tuberculosisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FURG (RI FURG)instname:Universidade Federal do Rio Grande (FURG)instacron:FURGORIGINALThe multidrug transporters belonging to Major Facilitator Superfamily (MFS) in Mycobacterium tuberculosis.pdfThe multidrug transporters belonging to Major Facilitator Superfamily (MFS) in Mycobacterium tuberculosis.pdfapplication/pdf195487https://repositorio.furg.br/bitstream/1/3103/1/The%20multidrug%20transporters%20belonging%20to%20Major%20Facilitator%20Superfamily%20%28MFS%29%20in%20Mycobacterium%20tuberculosis.pdfac47eb86a5476491ac854555f50df8ceMD51open accessLICENSElicense.txtlicense.txttext/plain; charset=utf-81724https://repositorio.furg.br/bitstream/1/3103/2/license.txt5b92b9704b4f13242d70e45ddef35a68MD52open access1/31032013-01-17 18:10:44.319open accessoai:repositorio.furg.br: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Repositório InstitucionalPUBhttps://repositorio.furg.br/oai/request || http://200.19.254.174/oai/requestopendoar:2013-01-17T20:10:44Repositório Institucional da FURG (RI FURG) - Universidade Federal do Rio Grande (FURG)false |
dc.title.pt_BR.fl_str_mv |
The multidrug transporters belonging to major facilitator superfamily (MFS) in mycobacterium tuberculosis |
title |
The multidrug transporters belonging to major facilitator superfamily (MFS) in mycobacterium tuberculosis |
spellingShingle |
The multidrug transporters belonging to major facilitator superfamily (MFS) in mycobacterium tuberculosis Rossi, Edda de |
title_short |
The multidrug transporters belonging to major facilitator superfamily (MFS) in mycobacterium tuberculosis |
title_full |
The multidrug transporters belonging to major facilitator superfamily (MFS) in mycobacterium tuberculosis |
title_fullStr |
The multidrug transporters belonging to major facilitator superfamily (MFS) in mycobacterium tuberculosis |
title_full_unstemmed |
The multidrug transporters belonging to major facilitator superfamily (MFS) in mycobacterium tuberculosis |
title_sort |
The multidrug transporters belonging to major facilitator superfamily (MFS) in mycobacterium tuberculosis |
author |
Rossi, Edda de |
author_facet |
Rossi, Edda de Arrigo, Patrizio Bellinzoni, Marco Silva, Pedro Eduardo Almeida da Martín, Carlos Aínsa, José Guglierame, Paola Riccardi, Giovanna |
author_role |
author |
author2 |
Arrigo, Patrizio Bellinzoni, Marco Silva, Pedro Eduardo Almeida da Martín, Carlos Aínsa, José Guglierame, Paola Riccardi, Giovanna |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Rossi, Edda de Arrigo, Patrizio Bellinzoni, Marco Silva, Pedro Eduardo Almeida da Martín, Carlos Aínsa, José Guglierame, Paola Riccardi, Giovanna |
description |
Background: Both intrinsic and acquired multidrug resistance play an important role in the insurgence of tuberculosis. Detailed knowledge of the molecular basis of drug recognition and transport by multidrug transport systems is required for the development of new antibiotics that are not extruded or of inhibitors that block the multidrug transporter and allow traditional antibiotics to be effective. Materials and Methods: We have undertaken the inventory of the drug transporters subfamily, included in the major facilitator superfamily (MFS), encoded by the complete genome of Mycobacterium tuberculosis (MTB). These proteins were identified on the basis of their characteristic stretches of amino acids and transmembrane segments (TMS) number. Conclusions: Genome analysis and searches of homology between the identified transporters and proteins characterized in other organisms revealed 16 open reading frames encoding putative drug efflux pumps belonging to MFS. In the case of two of them, we also have demonstrated that they function as drug efflux proteins. |
publishDate |
2002 |
dc.date.issued.fl_str_mv |
2002 |
dc.date.accessioned.fl_str_mv |
2013-01-17T20:10:44Z |
dc.date.available.fl_str_mv |
2013-01-17T20:10:44Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
ROSSI, Edda de et al. The multidrug transporters belonging to major facilitator superfamily (MFS) in mycobacterium tuberculosis. Molecular Medicine, v. 8, n.11, p. 714-724, 2002. Disponível em: <http://130.203.133.150/viewdoc/summary;jsessionid=13E00EC2F38B4742D96AE349BE46AF22?doi=10.1.1.222.9957>. Acesso em: 18 set. 2012. |
dc.identifier.uri.fl_str_mv |
http://repositorio.furg.br/handle/1/3103 |
identifier_str_mv |
ROSSI, Edda de et al. The multidrug transporters belonging to major facilitator superfamily (MFS) in mycobacterium tuberculosis. Molecular Medicine, v. 8, n.11, p. 714-724, 2002. Disponível em: <http://130.203.133.150/viewdoc/summary;jsessionid=13E00EC2F38B4742D96AE349BE46AF22?doi=10.1.1.222.9957>. Acesso em: 18 set. 2012. |
url |
http://repositorio.furg.br/handle/1/3103 |
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eng |
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eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
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