Effect of low density lipoprotein on the quality of cryopreserved dog semen
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FURG (RI FURG) |
Texto Completo: | http://repositorio.furg.br/handle/1/84 |
Resumo: | Egg yolk is included in extenders for semen cryopreservation due to its protective effect against cold shock,which is attributed to the presence of low density lipoprotein (LDL). This study evaluates how semen quality is affected by using LDL as a replacement for egg yolk in extenders for cooled and frozen dog semen. In Experiment 1 semen was extended in TRIS–glucoseat5ºC, in four treatments: 20% egg yolk (T1); 6% (T2); 8% (T3); and 10% LDL (T4). Spermmotility and membrane integrity after 24, 48, 72 and 96 hand the 50% conservation rate of motil espermatozoa (50M) were evaluated. The 50 M was less for T1 than for the other treatments (P <0.01), but T2–T4 did not differ (P >0.05). In Experiment 2, glycerol at 10% was included in the freezing extender, in treatments similar to those from Experiment 1. Spermmotility and membrane integrity did not differ for T2, T3 and T4 at any period in Experiment 1 and after thawing in Experiment 2 (P >0.05), but were greater for all LDL treatments than for T1 (P <0.01), in both experiments. Thus, LDL can replace egg yolk in the composition of the TRIS–glucose extender for cooled or frozen dog semen. |
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Effect of low density lipoprotein on the quality of cryopreserved dog semenLow density lipoproteinSemen extendersCryopreservationDog semenEgg yolk is included in extenders for semen cryopreservation due to its protective effect against cold shock,which is attributed to the presence of low density lipoprotein (LDL). This study evaluates how semen quality is affected by using LDL as a replacement for egg yolk in extenders for cooled and frozen dog semen. In Experiment 1 semen was extended in TRIS–glucoseat5ºC, in four treatments: 20% egg yolk (T1); 6% (T2); 8% (T3); and 10% LDL (T4). Spermmotility and membrane integrity after 24, 48, 72 and 96 hand the 50% conservation rate of motil espermatozoa (50M) were evaluated. The 50 M was less for T1 than for the other treatments (P <0.01), but T2–T4 did not differ (P >0.05). In Experiment 2, glycerol at 10% was included in the freezing extender, in treatments similar to those from Experiment 1. Spermmotility and membrane integrity did not differ for T2, T3 and T4 at any period in Experiment 1 and after thawing in Experiment 2 (P >0.05), but were greater for all LDL treatments than for T1 (P <0.01), in both experiments. Thus, LDL can replace egg yolk in the composition of the TRIS–glucose extender for cooled or frozen dog semen.2010-10-02T17:28:23Z2010-10-02T17:28:23Z2008info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfVARELA JUNIOR, A. S. et al. Effect of low density lipoprotein on the quality of cryopreserved dog semen. Animal Reproduction Science , v. 115, p. 323-327, 2009.0378-4320http://repositorio.furg.br/handle/1/84engVarela Junior, Antonio SergioCorcini, Carine DahlUlguim, Rafael da RosaAlvarenga, Marcus ViniciusBianchi, IvanCorrêa, Marcio NunesLucia Junior, ThomazDeschamps, João Carlosinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FURG (RI FURG)instname:Universidade Federal do Rio Grande (FURG)instacron:FURG2011-08-12T19:01:07Zoai:repositorio.furg.br:1/84Repositório InstitucionalPUBhttps://repositorio.furg.br/oai/request || http://200.19.254.174/oai/requestopendoar:2011-08-12T19:01:07Repositório Institucional da FURG (RI FURG) - Universidade Federal do Rio Grande (FURG)false |
dc.title.none.fl_str_mv |
Effect of low density lipoprotein on the quality of cryopreserved dog semen |
title |
Effect of low density lipoprotein on the quality of cryopreserved dog semen |
spellingShingle |
Effect of low density lipoprotein on the quality of cryopreserved dog semen Varela Junior, Antonio Sergio Low density lipoprotein Semen extenders Cryopreservation Dog semen |
title_short |
Effect of low density lipoprotein on the quality of cryopreserved dog semen |
title_full |
Effect of low density lipoprotein on the quality of cryopreserved dog semen |
title_fullStr |
Effect of low density lipoprotein on the quality of cryopreserved dog semen |
title_full_unstemmed |
Effect of low density lipoprotein on the quality of cryopreserved dog semen |
title_sort |
Effect of low density lipoprotein on the quality of cryopreserved dog semen |
author |
Varela Junior, Antonio Sergio |
author_facet |
Varela Junior, Antonio Sergio Corcini, Carine Dahl Ulguim, Rafael da Rosa Alvarenga, Marcus Vinicius Bianchi, Ivan Corrêa, Marcio Nunes Lucia Junior, Thomaz Deschamps, João Carlos |
author_role |
author |
author2 |
Corcini, Carine Dahl Ulguim, Rafael da Rosa Alvarenga, Marcus Vinicius Bianchi, Ivan Corrêa, Marcio Nunes Lucia Junior, Thomaz Deschamps, João Carlos |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Varela Junior, Antonio Sergio Corcini, Carine Dahl Ulguim, Rafael da Rosa Alvarenga, Marcus Vinicius Bianchi, Ivan Corrêa, Marcio Nunes Lucia Junior, Thomaz Deschamps, João Carlos |
dc.subject.por.fl_str_mv |
Low density lipoprotein Semen extenders Cryopreservation Dog semen |
topic |
Low density lipoprotein Semen extenders Cryopreservation Dog semen |
description |
Egg yolk is included in extenders for semen cryopreservation due to its protective effect against cold shock,which is attributed to the presence of low density lipoprotein (LDL). This study evaluates how semen quality is affected by using LDL as a replacement for egg yolk in extenders for cooled and frozen dog semen. In Experiment 1 semen was extended in TRIS–glucoseat5ºC, in four treatments: 20% egg yolk (T1); 6% (T2); 8% (T3); and 10% LDL (T4). Spermmotility and membrane integrity after 24, 48, 72 and 96 hand the 50% conservation rate of motil espermatozoa (50M) were evaluated. The 50 M was less for T1 than for the other treatments (P <0.01), but T2–T4 did not differ (P >0.05). In Experiment 2, glycerol at 10% was included in the freezing extender, in treatments similar to those from Experiment 1. Spermmotility and membrane integrity did not differ for T2, T3 and T4 at any period in Experiment 1 and after thawing in Experiment 2 (P >0.05), but were greater for all LDL treatments than for T1 (P <0.01), in both experiments. Thus, LDL can replace egg yolk in the composition of the TRIS–glucose extender for cooled or frozen dog semen. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008 2010-10-02T17:28:23Z 2010-10-02T17:28:23Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
VARELA JUNIOR, A. S. et al. Effect of low density lipoprotein on the quality of cryopreserved dog semen. Animal Reproduction Science , v. 115, p. 323-327, 2009. 0378-4320 http://repositorio.furg.br/handle/1/84 |
identifier_str_mv |
VARELA JUNIOR, A. S. et al. Effect of low density lipoprotein on the quality of cryopreserved dog semen. Animal Reproduction Science , v. 115, p. 323-327, 2009. 0378-4320 |
url |
http://repositorio.furg.br/handle/1/84 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da FURG (RI FURG) instname:Universidade Federal do Rio Grande (FURG) instacron:FURG |
instname_str |
Universidade Federal do Rio Grande (FURG) |
instacron_str |
FURG |
institution |
FURG |
reponame_str |
Repositório Institucional da FURG (RI FURG) |
collection |
Repositório Institucional da FURG (RI FURG) |
repository.name.fl_str_mv |
Repositório Institucional da FURG (RI FURG) - Universidade Federal do Rio Grande (FURG) |
repository.mail.fl_str_mv |
|
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1813187242367123456 |