Effect of low density lipoprotein on the quality of cryopreserved dog semen

Detalhes bibliográficos
Autor(a) principal: Varela Junior, Antonio Sergio
Data de Publicação: 2008
Outros Autores: Corcini, Carine Dahl, Ulguim, Rafael da Rosa, Alvarenga, Marcus Vinicius, Bianchi, Ivan, Corrêa, Marcio Nunes, Lucia Junior, Thomaz, Deschamps, João Carlos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FURG (RI FURG)
Texto Completo: http://repositorio.furg.br/handle/1/84
Resumo: Egg yolk is included in extenders for semen cryopreservation due to its protective effect against cold shock,which is attributed to the presence of low density lipoprotein (LDL). This study evaluates how semen quality is affected by using LDL as a replacement for egg yolk in extenders for cooled and frozen dog semen. In Experiment 1 semen was extended in TRIS–glucoseat5ºC, in four treatments: 20% egg yolk (T1); 6% (T2); 8% (T3); and 10% LDL (T4). Spermmotility and membrane integrity after 24, 48, 72 and 96 hand the 50% conservation rate of motil espermatozoa (50M) were evaluated. The 50 M was less for T1 than for the other treatments (P <0.01), but T2–T4 did not differ (P >0.05). In Experiment 2, glycerol at 10% was included in the freezing extender, in treatments similar to those from Experiment 1. Spermmotility and membrane integrity did not differ for T2, T3 and T4 at any period in Experiment 1 and after thawing in Experiment 2 (P >0.05), but were greater for all LDL treatments than for T1 (P <0.01), in both experiments. Thus, LDL can replace egg yolk in the composition of the TRIS–glucose extender for cooled or frozen dog semen.
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spelling Effect of low density lipoprotein on the quality of cryopreserved dog semenLow density lipoproteinSemen extendersCryopreservationDog semenEgg yolk is included in extenders for semen cryopreservation due to its protective effect against cold shock,which is attributed to the presence of low density lipoprotein (LDL). This study evaluates how semen quality is affected by using LDL as a replacement for egg yolk in extenders for cooled and frozen dog semen. In Experiment 1 semen was extended in TRIS–glucoseat5ºC, in four treatments: 20% egg yolk (T1); 6% (T2); 8% (T3); and 10% LDL (T4). Spermmotility and membrane integrity after 24, 48, 72 and 96 hand the 50% conservation rate of motil espermatozoa (50M) were evaluated. The 50 M was less for T1 than for the other treatments (P <0.01), but T2–T4 did not differ (P >0.05). In Experiment 2, glycerol at 10% was included in the freezing extender, in treatments similar to those from Experiment 1. Spermmotility and membrane integrity did not differ for T2, T3 and T4 at any period in Experiment 1 and after thawing in Experiment 2 (P >0.05), but were greater for all LDL treatments than for T1 (P <0.01), in both experiments. Thus, LDL can replace egg yolk in the composition of the TRIS–glucose extender for cooled or frozen dog semen.2010-10-02T17:28:23Z2010-10-02T17:28:23Z2008info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfVARELA JUNIOR, A. S. et al. Effect of low density lipoprotein on the quality of cryopreserved dog semen. Animal Reproduction Science , v. 115, p. 323-327, 2009.0378-4320http://repositorio.furg.br/handle/1/84engVarela Junior, Antonio SergioCorcini, Carine DahlUlguim, Rafael da RosaAlvarenga, Marcus ViniciusBianchi, IvanCorrêa, Marcio NunesLucia Junior, ThomazDeschamps, João Carlosinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FURG (RI FURG)instname:Universidade Federal do Rio Grande (FURG)instacron:FURG2011-08-12T19:01:07Zoai:repositorio.furg.br:1/84Repositório InstitucionalPUBhttps://repositorio.furg.br/oai/request || http://200.19.254.174/oai/requestopendoar:2011-08-12T19:01:07Repositório Institucional da FURG (RI FURG) - Universidade Federal do Rio Grande (FURG)false
dc.title.none.fl_str_mv Effect of low density lipoprotein on the quality of cryopreserved dog semen
title Effect of low density lipoprotein on the quality of cryopreserved dog semen
spellingShingle Effect of low density lipoprotein on the quality of cryopreserved dog semen
Varela Junior, Antonio Sergio
Low density lipoprotein
Semen extenders
Cryopreservation
Dog semen
title_short Effect of low density lipoprotein on the quality of cryopreserved dog semen
title_full Effect of low density lipoprotein on the quality of cryopreserved dog semen
title_fullStr Effect of low density lipoprotein on the quality of cryopreserved dog semen
title_full_unstemmed Effect of low density lipoprotein on the quality of cryopreserved dog semen
title_sort Effect of low density lipoprotein on the quality of cryopreserved dog semen
author Varela Junior, Antonio Sergio
author_facet Varela Junior, Antonio Sergio
Corcini, Carine Dahl
Ulguim, Rafael da Rosa
Alvarenga, Marcus Vinicius
Bianchi, Ivan
Corrêa, Marcio Nunes
Lucia Junior, Thomaz
Deschamps, João Carlos
author_role author
author2 Corcini, Carine Dahl
Ulguim, Rafael da Rosa
Alvarenga, Marcus Vinicius
Bianchi, Ivan
Corrêa, Marcio Nunes
Lucia Junior, Thomaz
Deschamps, João Carlos
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Varela Junior, Antonio Sergio
Corcini, Carine Dahl
Ulguim, Rafael da Rosa
Alvarenga, Marcus Vinicius
Bianchi, Ivan
Corrêa, Marcio Nunes
Lucia Junior, Thomaz
Deschamps, João Carlos
dc.subject.por.fl_str_mv Low density lipoprotein
Semen extenders
Cryopreservation
Dog semen
topic Low density lipoprotein
Semen extenders
Cryopreservation
Dog semen
description Egg yolk is included in extenders for semen cryopreservation due to its protective effect against cold shock,which is attributed to the presence of low density lipoprotein (LDL). This study evaluates how semen quality is affected by using LDL as a replacement for egg yolk in extenders for cooled and frozen dog semen. In Experiment 1 semen was extended in TRIS–glucoseat5ºC, in four treatments: 20% egg yolk (T1); 6% (T2); 8% (T3); and 10% LDL (T4). Spermmotility and membrane integrity after 24, 48, 72 and 96 hand the 50% conservation rate of motil espermatozoa (50M) were evaluated. The 50 M was less for T1 than for the other treatments (P <0.01), but T2–T4 did not differ (P >0.05). In Experiment 2, glycerol at 10% was included in the freezing extender, in treatments similar to those from Experiment 1. Spermmotility and membrane integrity did not differ for T2, T3 and T4 at any period in Experiment 1 and after thawing in Experiment 2 (P >0.05), but were greater for all LDL treatments than for T1 (P <0.01), in both experiments. Thus, LDL can replace egg yolk in the composition of the TRIS–glucose extender for cooled or frozen dog semen.
publishDate 2008
dc.date.none.fl_str_mv 2008
2010-10-02T17:28:23Z
2010-10-02T17:28:23Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv VARELA JUNIOR, A. S. et al. Effect of low density lipoprotein on the quality of cryopreserved dog semen. Animal Reproduction Science , v. 115, p. 323-327, 2009.
0378-4320
http://repositorio.furg.br/handle/1/84
identifier_str_mv VARELA JUNIOR, A. S. et al. Effect of low density lipoprotein on the quality of cryopreserved dog semen. Animal Reproduction Science , v. 115, p. 323-327, 2009.
0378-4320
url http://repositorio.furg.br/handle/1/84
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Institucional da FURG (RI FURG)
instname:Universidade Federal do Rio Grande (FURG)
instacron:FURG
instname_str Universidade Federal do Rio Grande (FURG)
instacron_str FURG
institution FURG
reponame_str Repositório Institucional da FURG (RI FURG)
collection Repositório Institucional da FURG (RI FURG)
repository.name.fl_str_mv Repositório Institucional da FURG (RI FURG) - Universidade Federal do Rio Grande (FURG)
repository.mail.fl_str_mv
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