Aggregate formation in axenic and microbial co-inoculated batch cultures of aulacoseira granulata (bacillariophyceae)
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FURG (RI FURG) |
Texto Completo: | http://repositorio.furg.br/handle/1/2837 |
Resumo: | Aggregate formation in axenic and microbial co-inoculated batch cultures of Aulacoseira granulata (Bacillariophyceae). Aggregate formation involving diatoms is a well-known process, especially in the marine environment. The aim of this paper was to observe natural aggregates formation in axenic and microbial co-inoculated cultures of the freshwater diatom Aulacoseira granulata. The growth of bacteria and A. granulata were also monitored, as were qualitative alterations in the composition of the extracellular polysaccharide (EPS) produced by A. granulata in both axenic and microbial co-inoculated cultures. Aggregates were naturally formed in both conditions and our results suggested that the aggregation process increased significantly in the presence of a microbial community from Barra Bonita Reservoir. A. granulata biomass formation also significantly increased along bacterial growth and the EPS composition found in the microbial co-inoculated cultures was different to that of the axenic cultures. Rhamnose proportion increased around 50 % in microbial co-inoculated cultures, probably due to bacterial activity, increasing the hydrophobic properties of the EPS. The aggregation increase in the microbial co-inoculated cultures was correlated to the enhancement of the EPS hydrophobic nature, as well as to the biomass increase of A. granulata. |
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Aggregate formation in axenic and microbial co-inoculated batch cultures of aulacoseira granulata (bacillariophyceae)Formação de agregados em culturas de Aulacoseira granulata (Bacillariophyceae) axênicas e inoculadas com bactériasAulacoseira granulataAggregatesExtracellular polysaccharideMicrobial degradationBacterial growthAulacoseira granulataAgregadosPolissacarídeo extracelularDegradação microbianaCrescimento bacterianoAggregate formation in axenic and microbial co-inoculated batch cultures of Aulacoseira granulata (Bacillariophyceae). Aggregate formation involving diatoms is a well-known process, especially in the marine environment. The aim of this paper was to observe natural aggregates formation in axenic and microbial co-inoculated cultures of the freshwater diatom Aulacoseira granulata. The growth of bacteria and A. granulata were also monitored, as were qualitative alterations in the composition of the extracellular polysaccharide (EPS) produced by A. granulata in both axenic and microbial co-inoculated cultures. Aggregates were naturally formed in both conditions and our results suggested that the aggregation process increased significantly in the presence of a microbial community from Barra Bonita Reservoir. A. granulata biomass formation also significantly increased along bacterial growth and the EPS composition found in the microbial co-inoculated cultures was different to that of the axenic cultures. Rhamnose proportion increased around 50 % in microbial co-inoculated cultures, probably due to bacterial activity, increasing the hydrophobic properties of the EPS. The aggregation increase in the microbial co-inoculated cultures was correlated to the enhancement of the EPS hydrophobic nature, as well as to the biomass increase of A. granulata.A formação de agregados envolvendo diatomáceas é já um processo bem conhecido, especialmente em ambientes marinhos. O objetivo deste trabalho foi observar a formação espontânea de agregados em culturas de Aulacoseira granulata axênicas e inoculadas com bactérias. O crescimento de bactérias e de A. granulata foi monitorado bem como as alterações qualitativas, causadas pela atividade bacteriana, na composição do polissacarídeo extracelular (EPS) liberado pela diatomácea em ambas as culturas. A formação de agregados ocorreu em ambas as culturas, mas os resultados sugerem que a agregação aumenta significantemente na presença de bactérias de populações oriundas do reservatório de Barra Bonita, também o local de origem da diatomácea. A biomassa de A. granulata aumentou significantemente na presença de bactérias e a composição do EPS nas culturas contaminadas foi diferente daquele das culturas axênicas. A atividade bacteriana aumentou a porcentagem do monossacarídeo Ramnose em cerca de 50 % nas culturas contaminadas, aumentando as propriedades hidrofóbicas do EPS. Assumimos que o aumento da hidrofobicidade bem como o aumento da biomassa de A. granulata nas culturas contaminadas foram os fatores responsáveis pelo aumento da agregação dos filamentos de A. granulata nas culturas contaminadas.2012-11-27T23:38:52Z2012-11-27T23:38:52Z2006info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfVIEIRA, Armando Augusto Henriques; GIROLDO, Danilo; ORTOLANO, Pedro Ivo Coelho. Aggregate formation in axenic and microbial co-inoculated batch cultures of Aulacoseira granulata (Bacillariophyceae). Acta Limnologica Brasiliensia, v. 18, n.1, p. 1-7, 2006. Disponível em: <http://ablimno.org.br/acta/pdf/acta_limnologica_contents1801E_files/Art01_18(1).pdf>. Acesso em: 22 nov. 2012.http://repositorio.furg.br/handle/1/2837engVieira, Armando Augusto HenriquesGiroldo, DaniloOrtolano, Pedro Ivo Coelhoinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FURG (RI FURG)instname:Universidade Federal do Rio Grande (FURG)instacron:FURG2012-11-27T23:38:52Zoai:repositorio.furg.br:1/2837Repositório InstitucionalPUBhttps://repositorio.furg.br/oai/request || http://200.19.254.174/oai/requestopendoar:2012-11-27T23:38:52Repositório Institucional da FURG (RI FURG) - Universidade Federal do Rio Grande (FURG)false |
dc.title.none.fl_str_mv |
Aggregate formation in axenic and microbial co-inoculated batch cultures of aulacoseira granulata (bacillariophyceae) Formação de agregados em culturas de Aulacoseira granulata (Bacillariophyceae) axênicas e inoculadas com bactérias |
title |
Aggregate formation in axenic and microbial co-inoculated batch cultures of aulacoseira granulata (bacillariophyceae) |
spellingShingle |
Aggregate formation in axenic and microbial co-inoculated batch cultures of aulacoseira granulata (bacillariophyceae) Vieira, Armando Augusto Henriques Aulacoseira granulata Aggregates Extracellular polysaccharide Microbial degradation Bacterial growth Aulacoseira granulata Agregados Polissacarídeo extracelular Degradação microbiana Crescimento bacteriano |
title_short |
Aggregate formation in axenic and microbial co-inoculated batch cultures of aulacoseira granulata (bacillariophyceae) |
title_full |
Aggregate formation in axenic and microbial co-inoculated batch cultures of aulacoseira granulata (bacillariophyceae) |
title_fullStr |
Aggregate formation in axenic and microbial co-inoculated batch cultures of aulacoseira granulata (bacillariophyceae) |
title_full_unstemmed |
Aggregate formation in axenic and microbial co-inoculated batch cultures of aulacoseira granulata (bacillariophyceae) |
title_sort |
Aggregate formation in axenic and microbial co-inoculated batch cultures of aulacoseira granulata (bacillariophyceae) |
author |
Vieira, Armando Augusto Henriques |
author_facet |
Vieira, Armando Augusto Henriques Giroldo, Danilo Ortolano, Pedro Ivo Coelho |
author_role |
author |
author2 |
Giroldo, Danilo Ortolano, Pedro Ivo Coelho |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Vieira, Armando Augusto Henriques Giroldo, Danilo Ortolano, Pedro Ivo Coelho |
dc.subject.por.fl_str_mv |
Aulacoseira granulata Aggregates Extracellular polysaccharide Microbial degradation Bacterial growth Aulacoseira granulata Agregados Polissacarídeo extracelular Degradação microbiana Crescimento bacteriano |
topic |
Aulacoseira granulata Aggregates Extracellular polysaccharide Microbial degradation Bacterial growth Aulacoseira granulata Agregados Polissacarídeo extracelular Degradação microbiana Crescimento bacteriano |
description |
Aggregate formation in axenic and microbial co-inoculated batch cultures of Aulacoseira granulata (Bacillariophyceae). Aggregate formation involving diatoms is a well-known process, especially in the marine environment. The aim of this paper was to observe natural aggregates formation in axenic and microbial co-inoculated cultures of the freshwater diatom Aulacoseira granulata. The growth of bacteria and A. granulata were also monitored, as were qualitative alterations in the composition of the extracellular polysaccharide (EPS) produced by A. granulata in both axenic and microbial co-inoculated cultures. Aggregates were naturally formed in both conditions and our results suggested that the aggregation process increased significantly in the presence of a microbial community from Barra Bonita Reservoir. A. granulata biomass formation also significantly increased along bacterial growth and the EPS composition found in the microbial co-inoculated cultures was different to that of the axenic cultures. Rhamnose proportion increased around 50 % in microbial co-inoculated cultures, probably due to bacterial activity, increasing the hydrophobic properties of the EPS. The aggregation increase in the microbial co-inoculated cultures was correlated to the enhancement of the EPS hydrophobic nature, as well as to the biomass increase of A. granulata. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006 2012-11-27T23:38:52Z 2012-11-27T23:38:52Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
VIEIRA, Armando Augusto Henriques; GIROLDO, Danilo; ORTOLANO, Pedro Ivo Coelho. Aggregate formation in axenic and microbial co-inoculated batch cultures of Aulacoseira granulata (Bacillariophyceae). Acta Limnologica Brasiliensia, v. 18, n.1, p. 1-7, 2006. Disponível em: <http://ablimno.org.br/acta/pdf/acta_limnologica_contents1801E_files/Art01_18(1).pdf>. Acesso em: 22 nov. 2012. http://repositorio.furg.br/handle/1/2837 |
identifier_str_mv |
VIEIRA, Armando Augusto Henriques; GIROLDO, Danilo; ORTOLANO, Pedro Ivo Coelho. Aggregate formation in axenic and microbial co-inoculated batch cultures of Aulacoseira granulata (Bacillariophyceae). Acta Limnologica Brasiliensia, v. 18, n.1, p. 1-7, 2006. Disponível em: <http://ablimno.org.br/acta/pdf/acta_limnologica_contents1801E_files/Art01_18(1).pdf>. Acesso em: 22 nov. 2012. |
url |
http://repositorio.furg.br/handle/1/2837 |
dc.language.iso.fl_str_mv |
eng |
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eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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reponame:Repositório Institucional da FURG (RI FURG) instname:Universidade Federal do Rio Grande (FURG) instacron:FURG |
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Universidade Federal do Rio Grande (FURG) |
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FURG |
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FURG |
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Repositório Institucional da FURG (RI FURG) |
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Repositório Institucional da FURG (RI FURG) |
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Repositório Institucional da FURG (RI FURG) - Universidade Federal do Rio Grande (FURG) |
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