Parameters optimization for enzymatic assays using experimental design
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FURG (RI FURG) |
Texto Completo: | http://repositorio.furg.br/handle/1/4575 |
Resumo: | The conditions for maximization enzymatic activity were determined using experimental design and inulinase from Kluyveromyces marxianus ATCC 16045. The effects of substrate concentration (sucrose and inulin), pH and temperature on inulinase activity were verified using four factorial design and surface response analysis. Using sucrose as substrate. It has bean shown that the effects sucrose on enzymatic activity is not statistically significant and the best condition for the highest activity (110 U/mL) was achieved with temperature between 60°C and 68°C and pH between 4.5 and 5.0. Using inulin as substrate it was verified that temperature is the only variable statistically significant and the maximum activity was 7.3 U/mL at temperature between 50°C and 51°C. |
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Parameters optimization for enzymatic assays using experimental designInulinaseKluyveromyces marxianusActivityFactorial designResponse surface analysisThe conditions for maximization enzymatic activity were determined using experimental design and inulinase from Kluyveromyces marxianus ATCC 16045. The effects of substrate concentration (sucrose and inulin), pH and temperature on inulinase activity were verified using four factorial design and surface response analysis. Using sucrose as substrate. It has bean shown that the effects sucrose on enzymatic activity is not statistically significant and the best condition for the highest activity (110 U/mL) was achieved with temperature between 60°C and 68°C and pH between 4.5 and 5.0. Using inulin as substrate it was verified that temperature is the only variable statistically significant and the maximum activity was 7.3 U/mL at temperature between 50°C and 51°C.2014-09-18T11:47:10Z2014-09-18T11:47:10Z2006info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfBURKERT, Janaína Fernandes de Medeiros et al. Parameters optimization for enzymatic assays using experimental design. Brazilian Journal of Chemical Engineering, v. 23, n. 2, p. 163-170, 2006. Disponível em: <http://www.scielo.br/pdf/bjce/v23n2/29920.pdf>. Acesso em: 16 set. 2014.0104-6632http://repositorio.furg.br/handle/1/4575engBurkert, Janaina Fernandes de MedeirosKalil, Susana JulianoMaugeri Filho, FranciscoRodrigues, Maria Isabelinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FURG (RI FURG)instname:Universidade Federal do Rio Grande (FURG)instacron:FURG2019-11-13T23:27:34Zoai:repositorio.furg.br:1/4575Repositório InstitucionalPUBhttps://repositorio.furg.br/oai/request || http://200.19.254.174/oai/requestopendoar:2019-11-13T23:27:34Repositório Institucional da FURG (RI FURG) - Universidade Federal do Rio Grande (FURG)false |
dc.title.none.fl_str_mv |
Parameters optimization for enzymatic assays using experimental design |
title |
Parameters optimization for enzymatic assays using experimental design |
spellingShingle |
Parameters optimization for enzymatic assays using experimental design Burkert, Janaina Fernandes de Medeiros Inulinase Kluyveromyces marxianus Activity Factorial design Response surface analysis |
title_short |
Parameters optimization for enzymatic assays using experimental design |
title_full |
Parameters optimization for enzymatic assays using experimental design |
title_fullStr |
Parameters optimization for enzymatic assays using experimental design |
title_full_unstemmed |
Parameters optimization for enzymatic assays using experimental design |
title_sort |
Parameters optimization for enzymatic assays using experimental design |
author |
Burkert, Janaina Fernandes de Medeiros |
author_facet |
Burkert, Janaina Fernandes de Medeiros Kalil, Susana Juliano Maugeri Filho, Francisco Rodrigues, Maria Isabel |
author_role |
author |
author2 |
Kalil, Susana Juliano Maugeri Filho, Francisco Rodrigues, Maria Isabel |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Burkert, Janaina Fernandes de Medeiros Kalil, Susana Juliano Maugeri Filho, Francisco Rodrigues, Maria Isabel |
dc.subject.por.fl_str_mv |
Inulinase Kluyveromyces marxianus Activity Factorial design Response surface analysis |
topic |
Inulinase Kluyveromyces marxianus Activity Factorial design Response surface analysis |
description |
The conditions for maximization enzymatic activity were determined using experimental design and inulinase from Kluyveromyces marxianus ATCC 16045. The effects of substrate concentration (sucrose and inulin), pH and temperature on inulinase activity were verified using four factorial design and surface response analysis. Using sucrose as substrate. It has bean shown that the effects sucrose on enzymatic activity is not statistically significant and the best condition for the highest activity (110 U/mL) was achieved with temperature between 60°C and 68°C and pH between 4.5 and 5.0. Using inulin as substrate it was verified that temperature is the only variable statistically significant and the maximum activity was 7.3 U/mL at temperature between 50°C and 51°C. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006 2014-09-18T11:47:10Z 2014-09-18T11:47:10Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
BURKERT, Janaína Fernandes de Medeiros et al. Parameters optimization for enzymatic assays using experimental design. Brazilian Journal of Chemical Engineering, v. 23, n. 2, p. 163-170, 2006. Disponível em: <http://www.scielo.br/pdf/bjce/v23n2/29920.pdf>. Acesso em: 16 set. 2014. 0104-6632 http://repositorio.furg.br/handle/1/4575 |
identifier_str_mv |
BURKERT, Janaína Fernandes de Medeiros et al. Parameters optimization for enzymatic assays using experimental design. Brazilian Journal of Chemical Engineering, v. 23, n. 2, p. 163-170, 2006. Disponível em: <http://www.scielo.br/pdf/bjce/v23n2/29920.pdf>. Acesso em: 16 set. 2014. 0104-6632 |
url |
http://repositorio.furg.br/handle/1/4575 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da FURG (RI FURG) instname:Universidade Federal do Rio Grande (FURG) instacron:FURG |
instname_str |
Universidade Federal do Rio Grande (FURG) |
instacron_str |
FURG |
institution |
FURG |
reponame_str |
Repositório Institucional da FURG (RI FURG) |
collection |
Repositório Institucional da FURG (RI FURG) |
repository.name.fl_str_mv |
Repositório Institucional da FURG (RI FURG) - Universidade Federal do Rio Grande (FURG) |
repository.mail.fl_str_mv |
|
_version_ |
1807384413702455296 |