Mitochondrial control region haplotypes of the South American sea lion Otaria flavescens (Shaw, 1800)
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FURG (RI FURG) |
Texto Completo: | http://repositorio.furg.br/handle/1/716 |
Resumo: | The South American sea lion, Otaria flavescens, is widely distributed along the Pacific and Atlantic coasts of South America. However, along the Brazilian coast, there are only two nonbreeding sites for the species (Refúgio de Vida Silvestre da Ilha dos Lobos and Refúgio de Vida Silvestre do Molhe Leste da Barra do Rio Grande), both in Southern Brazil. In this region, the species is continuously under the effect of anthropic activities, mainly those related to environmental contamination with organic and inorganic chemicals and fishery interactions. This paper reports, for the first time, the genetic diversity of O. flavescens found along the Southern Brazilian coast. A 287-bp fragment of the mitochondrial DNA control region (D-loop) was analyzed. Seven novel haplotypes were found in 56 individuals (OFA1-OFA7), with OFA1 being the most frequent (47.54%). Nucleotide diversity was moderate (π = 0.62%) and haplotype diversity was relatively low (67%). Furthermore, the median joining network analysis indicated that Brazilian haplotypes formed a reciprocal monophyletic clade when compared to the haplotypes from the Peruvian population on the Pacific coast. These two populations do not share haplotypes and may have become isolated some time back. Further genetic studies covering the entire species distribution are necessary to better understand the biological implications of the results reported here for the management and conservation of South American sea lions. |
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Artico, Liane de OliveiraBianchini, AdaltoSilva, Kleber Grübel daMonteiro, Danielle da SilveiraEstima, SérgioOliveira, Larissa Rosa deBonatto, Sandro LuisMarins, Luis Fernando Fernandes2011-06-17T03:59:30Z2011-06-17T03:59:30Z2010ARTICO, L. O. et al. Mitochondrial control region haplotypes of the South American sea lion Otaria flavescens (Shaw, 1800). Brazilian journal of medical and biological research, v. 43, p. 816-820, 2010. Disponível em: <http://www.scielo.br/pdf/bjmbr/v43n9/400.pdf>. Acesso em: 13 jun. 2011.0100-879Xhttp://repositorio.furg.br/handle/1/716The South American sea lion, Otaria flavescens, is widely distributed along the Pacific and Atlantic coasts of South America. However, along the Brazilian coast, there are only two nonbreeding sites for the species (Refúgio de Vida Silvestre da Ilha dos Lobos and Refúgio de Vida Silvestre do Molhe Leste da Barra do Rio Grande), both in Southern Brazil. In this region, the species is continuously under the effect of anthropic activities, mainly those related to environmental contamination with organic and inorganic chemicals and fishery interactions. This paper reports, for the first time, the genetic diversity of O. flavescens found along the Southern Brazilian coast. A 287-bp fragment of the mitochondrial DNA control region (D-loop) was analyzed. Seven novel haplotypes were found in 56 individuals (OFA1-OFA7), with OFA1 being the most frequent (47.54%). Nucleotide diversity was moderate (π = 0.62%) and haplotype diversity was relatively low (67%). Furthermore, the median joining network analysis indicated that Brazilian haplotypes formed a reciprocal monophyletic clade when compared to the haplotypes from the Peruvian population on the Pacific coast. These two populations do not share haplotypes and may have become isolated some time back. Further genetic studies covering the entire species distribution are necessary to better understand the biological implications of the results reported here for the management and conservation of South American sea lions.engOtaria flavescensmtDNAD-loopGenetic variabilityNew haplotypesMitochondrial control region haplotypes of the South American sea lion Otaria flavescens (Shaw, 1800)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FURG (RI FURG)instname:Universidade Federal do Rio Grande (FURG)instacron:FURGORIGINALMitochondrial control region haplotypes of the south american sea lion otaria flavescens (Shaw, 1800).pdfMitochondrial control region haplotypes of the south american sea lion otaria flavescens (Shaw, 1800).pdfapplication/pdf642130https://repositorio.furg.br/bitstream/1/716/1/Mitochondrial%20control%20region%20haplotypes%20of%20the%20south%20american%20sea%20lion%20otaria%20flavescens%20%28Shaw%2c%201800%29.pdf37d936d4dd2450db06bca305cdc2b02eMD51open accessLICENSElicense.txtlicense.txttext/plain; charset=utf-81724https://repositorio.furg.br/bitstream/1/716/2/license.txt5b92b9704b4f13242d70e45ddef35a68MD52open access1/7162011-08-08 16:20:22.026open accessoai:repositorio.furg.br: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Repositório InstitucionalPUBhttps://repositorio.furg.br/oai/request || http://200.19.254.174/oai/requestopendoar:2011-08-08T19:20:22Repositório Institucional da FURG (RI FURG) - Universidade Federal do Rio Grande (FURG)false |
dc.title.pt_BR.fl_str_mv |
Mitochondrial control region haplotypes of the South American sea lion Otaria flavescens (Shaw, 1800) |
title |
Mitochondrial control region haplotypes of the South American sea lion Otaria flavescens (Shaw, 1800) |
spellingShingle |
Mitochondrial control region haplotypes of the South American sea lion Otaria flavescens (Shaw, 1800) Artico, Liane de Oliveira Otaria flavescens mtDNA D-loop Genetic variability New haplotypes |
title_short |
Mitochondrial control region haplotypes of the South American sea lion Otaria flavescens (Shaw, 1800) |
title_full |
Mitochondrial control region haplotypes of the South American sea lion Otaria flavescens (Shaw, 1800) |
title_fullStr |
Mitochondrial control region haplotypes of the South American sea lion Otaria flavescens (Shaw, 1800) |
title_full_unstemmed |
Mitochondrial control region haplotypes of the South American sea lion Otaria flavescens (Shaw, 1800) |
title_sort |
Mitochondrial control region haplotypes of the South American sea lion Otaria flavescens (Shaw, 1800) |
author |
Artico, Liane de Oliveira |
author_facet |
Artico, Liane de Oliveira Bianchini, Adalto Silva, Kleber Grübel da Monteiro, Danielle da Silveira Estima, Sérgio Oliveira, Larissa Rosa de Bonatto, Sandro Luis Marins, Luis Fernando Fernandes |
author_role |
author |
author2 |
Bianchini, Adalto Silva, Kleber Grübel da Monteiro, Danielle da Silveira Estima, Sérgio Oliveira, Larissa Rosa de Bonatto, Sandro Luis Marins, Luis Fernando Fernandes |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Artico, Liane de Oliveira Bianchini, Adalto Silva, Kleber Grübel da Monteiro, Danielle da Silveira Estima, Sérgio Oliveira, Larissa Rosa de Bonatto, Sandro Luis Marins, Luis Fernando Fernandes |
dc.subject.por.fl_str_mv |
Otaria flavescens mtDNA D-loop Genetic variability New haplotypes |
topic |
Otaria flavescens mtDNA D-loop Genetic variability New haplotypes |
description |
The South American sea lion, Otaria flavescens, is widely distributed along the Pacific and Atlantic coasts of South America. However, along the Brazilian coast, there are only two nonbreeding sites for the species (Refúgio de Vida Silvestre da Ilha dos Lobos and Refúgio de Vida Silvestre do Molhe Leste da Barra do Rio Grande), both in Southern Brazil. In this region, the species is continuously under the effect of anthropic activities, mainly those related to environmental contamination with organic and inorganic chemicals and fishery interactions. This paper reports, for the first time, the genetic diversity of O. flavescens found along the Southern Brazilian coast. A 287-bp fragment of the mitochondrial DNA control region (D-loop) was analyzed. Seven novel haplotypes were found in 56 individuals (OFA1-OFA7), with OFA1 being the most frequent (47.54%). Nucleotide diversity was moderate (π = 0.62%) and haplotype diversity was relatively low (67%). Furthermore, the median joining network analysis indicated that Brazilian haplotypes formed a reciprocal monophyletic clade when compared to the haplotypes from the Peruvian population on the Pacific coast. These two populations do not share haplotypes and may have become isolated some time back. Further genetic studies covering the entire species distribution are necessary to better understand the biological implications of the results reported here for the management and conservation of South American sea lions. |
publishDate |
2010 |
dc.date.issued.fl_str_mv |
2010 |
dc.date.accessioned.fl_str_mv |
2011-06-17T03:59:30Z |
dc.date.available.fl_str_mv |
2011-06-17T03:59:30Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
ARTICO, L. O. et al. Mitochondrial control region haplotypes of the South American sea lion Otaria flavescens (Shaw, 1800). Brazilian journal of medical and biological research, v. 43, p. 816-820, 2010. Disponível em: <http://www.scielo.br/pdf/bjmbr/v43n9/400.pdf>. Acesso em: 13 jun. 2011. |
dc.identifier.uri.fl_str_mv |
http://repositorio.furg.br/handle/1/716 |
dc.identifier.issn.none.fl_str_mv |
0100-879X |
identifier_str_mv |
ARTICO, L. O. et al. Mitochondrial control region haplotypes of the South American sea lion Otaria flavescens (Shaw, 1800). Brazilian journal of medical and biological research, v. 43, p. 816-820, 2010. Disponível em: <http://www.scielo.br/pdf/bjmbr/v43n9/400.pdf>. Acesso em: 13 jun. 2011. 0100-879X |
url |
http://repositorio.furg.br/handle/1/716 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
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FURG |
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Repositório Institucional da FURG (RI FURG) |
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