Oyster Mushrooms Species Differentation Through Molecular Markers RAPD

Detalhes bibliográficos
Autor(a) principal: Fonseca, Gustavo Graciano
Data de Publicação: 2008
Outros Autores: Gandra, Eliezer Avila, Sclowitz, Liana Fossati, Corrêa, Ana Paula Antunes, Costa, Jorge Alberto Vieira, Levy, Jose Alberto
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FURG (RI FURG)
Texto Completo: http://repositorio.furg.br/handle/1/4475
Resumo: A RAPD methodology for differentiation and characterization of the fungal edible species Pleurotus ostreatus and Pleurotus sajor-caju was developed. Two of the most usual methods of DNA extraction were studied. The first was based in the simultaneous precipitation of proteins and polysaccharides by the presence of SDS (sodium dodecyl sulphate) and sodium acetate and the second by the use of cetyltrimethylammonium bromide (CTAB). Both methods presented satisfactory results and the best extraction products were obtained by the one that used SDS and sodium acetate. The study with RAPDs produced consistent DNA fragments, reproducible in different gels, serving as good markers for characterization and genetic differentiation of the studied species of mushrooms. The PCR conditions for obtaining RAPD markers were optimized with good interpretation
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spelling Oyster Mushrooms Species Differentation Through Molecular Markers RAPDDNA extractionPCRRAPDPleurotus ostreatusPleurotus sajor-cajuA RAPD methodology for differentiation and characterization of the fungal edible species Pleurotus ostreatus and Pleurotus sajor-caju was developed. Two of the most usual methods of DNA extraction were studied. The first was based in the simultaneous precipitation of proteins and polysaccharides by the presence of SDS (sodium dodecyl sulphate) and sodium acetate and the second by the use of cetyltrimethylammonium bromide (CTAB). Both methods presented satisfactory results and the best extraction products were obtained by the one that used SDS and sodium acetate. The study with RAPDs produced consistent DNA fragments, reproducible in different gels, serving as good markers for characterization and genetic differentiation of the studied species of mushrooms. The PCR conditions for obtaining RAPD markers were optimized with good interpretation2014-08-20T21:23:37Z2014-08-20T21:23:37Z2008info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfFONSECA, Gustavo Graciano. et al. Oyster Mushrooms Species Differentation Through Molecular Markers RAPD. International Journal of Plant Breeding and Genetics, v. 2, n. 1, p. 13-18, 2008. Disponível em: <http://scialert.net/qredirect.php?doi=ijpbg.2008.13.18&linkid=pdf>. Acesso em: 12 jul. 2014.1819-3595http://repositorio.furg.br/handle/1/4475engFonseca, Gustavo GracianoGandra, Eliezer AvilaSclowitz, Liana FossatiCorrêa, Ana Paula AntunesCosta, Jorge Alberto VieiraLevy, Jose Albertoinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FURG (RI FURG)instname:Universidade Federal do Rio Grande (FURG)instacron:FURG2014-08-20T21:23:37Zoai:repositorio.furg.br:1/4475Repositório InstitucionalPUBhttps://repositorio.furg.br/oai/request || http://200.19.254.174/oai/requestopendoar:2014-08-20T21:23:37Repositório Institucional da FURG (RI FURG) - Universidade Federal do Rio Grande (FURG)false
dc.title.none.fl_str_mv Oyster Mushrooms Species Differentation Through Molecular Markers RAPD
title Oyster Mushrooms Species Differentation Through Molecular Markers RAPD
spellingShingle Oyster Mushrooms Species Differentation Through Molecular Markers RAPD
Fonseca, Gustavo Graciano
DNA extraction
PCR
RAPD
Pleurotus ostreatus
Pleurotus sajor-caju
title_short Oyster Mushrooms Species Differentation Through Molecular Markers RAPD
title_full Oyster Mushrooms Species Differentation Through Molecular Markers RAPD
title_fullStr Oyster Mushrooms Species Differentation Through Molecular Markers RAPD
title_full_unstemmed Oyster Mushrooms Species Differentation Through Molecular Markers RAPD
title_sort Oyster Mushrooms Species Differentation Through Molecular Markers RAPD
author Fonseca, Gustavo Graciano
author_facet Fonseca, Gustavo Graciano
Gandra, Eliezer Avila
Sclowitz, Liana Fossati
Corrêa, Ana Paula Antunes
Costa, Jorge Alberto Vieira
Levy, Jose Alberto
author_role author
author2 Gandra, Eliezer Avila
Sclowitz, Liana Fossati
Corrêa, Ana Paula Antunes
Costa, Jorge Alberto Vieira
Levy, Jose Alberto
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Fonseca, Gustavo Graciano
Gandra, Eliezer Avila
Sclowitz, Liana Fossati
Corrêa, Ana Paula Antunes
Costa, Jorge Alberto Vieira
Levy, Jose Alberto
dc.subject.por.fl_str_mv DNA extraction
PCR
RAPD
Pleurotus ostreatus
Pleurotus sajor-caju
topic DNA extraction
PCR
RAPD
Pleurotus ostreatus
Pleurotus sajor-caju
description A RAPD methodology for differentiation and characterization of the fungal edible species Pleurotus ostreatus and Pleurotus sajor-caju was developed. Two of the most usual methods of DNA extraction were studied. The first was based in the simultaneous precipitation of proteins and polysaccharides by the presence of SDS (sodium dodecyl sulphate) and sodium acetate and the second by the use of cetyltrimethylammonium bromide (CTAB). Both methods presented satisfactory results and the best extraction products were obtained by the one that used SDS and sodium acetate. The study with RAPDs produced consistent DNA fragments, reproducible in different gels, serving as good markers for characterization and genetic differentiation of the studied species of mushrooms. The PCR conditions for obtaining RAPD markers were optimized with good interpretation
publishDate 2008
dc.date.none.fl_str_mv 2008
2014-08-20T21:23:37Z
2014-08-20T21:23:37Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv FONSECA, Gustavo Graciano. et al. Oyster Mushrooms Species Differentation Through Molecular Markers RAPD. International Journal of Plant Breeding and Genetics, v. 2, n. 1, p. 13-18, 2008. Disponível em: <http://scialert.net/qredirect.php?doi=ijpbg.2008.13.18&linkid=pdf>. Acesso em: 12 jul. 2014.
1819-3595
http://repositorio.furg.br/handle/1/4475
identifier_str_mv FONSECA, Gustavo Graciano. et al. Oyster Mushrooms Species Differentation Through Molecular Markers RAPD. International Journal of Plant Breeding and Genetics, v. 2, n. 1, p. 13-18, 2008. Disponível em: <http://scialert.net/qredirect.php?doi=ijpbg.2008.13.18&linkid=pdf>. Acesso em: 12 jul. 2014.
1819-3595
url http://repositorio.furg.br/handle/1/4475
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Institucional da FURG (RI FURG)
instname:Universidade Federal do Rio Grande (FURG)
instacron:FURG
instname_str Universidade Federal do Rio Grande (FURG)
instacron_str FURG
institution FURG
reponame_str Repositório Institucional da FURG (RI FURG)
collection Repositório Institucional da FURG (RI FURG)
repository.name.fl_str_mv Repositório Institucional da FURG (RI FURG) - Universidade Federal do Rio Grande (FURG)
repository.mail.fl_str_mv
_version_ 1813187275817746432

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