Sodium uptake in different life stages of crustaceans: the water flea Daphnia magna Strauss
Autor(a) principal: | |
---|---|
Data de Publicação: | 2008 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FURG (RI FURG) |
Texto Completo: | http://repositorio.furg.br/handle/1/721 |
Resumo: | The concentration-dependent kinetics and main mechanisms of whole-body Na+ uptake were assessed in neonate and adult water flea Daphnia magna Strauss acclimated to moderately hard water (0.6mmoll–1 NaCl, 1.0•mmol•l–1 CaCO3 and 0.15•mmol•l–1 MgSO4•7H2O; pH•8.2). Whole-body Na+ uptake is independent of the presence of Cl– in the external medium and kinetic parameters are dependent on the life stage. Adults have a lower maximum capacity of Na+ transport on a mass-specific basis but a higher affinity for Na+ when compared to neonates. Based on pharmacological analyses, mechanisms involved in whole-body Na+ uptake differ according to the life stage considered. In neonates, a proton pump-coupled Na+ channel appears to play an important role in the whole-body Na+ uptake at the apical membrane. However, they do not appear to contribute to whole-body Na+ uptake in adults, where only the Na+ channel seems to be present, associated with the Na+/H+ exchanger. In both cases, carbonic anhydrase contributes by providing H+ for the transporters. At the basolateral membrane of the salt-transporting epithelia of neonates, Na+ is pumped from the cells to the extracellular fluid by a Na+,K+-ATPase and a Na+/Cl– exchanger whereas K+ and Cl– move through specific channels. In adults, a Na+/K+/2Cl– cotransporter replaces the Na+/Cl– exchanger. Differential sensitivity of neonates and adults to iono- and osmoregulatory toxicants, such as metals, are discussed with respect to differences in whole-body Na+ uptake kinetics, as well as in the mechanisms of Na+ transport involved in the whole-body Na+ uptake in the two life stages. |
id |
FURG_f7f80accdc3f75292c98b13e0121b826 |
---|---|
oai_identifier_str |
oai:repositorio.furg.br:1/721 |
network_acronym_str |
FURG |
network_name_str |
Repositório Institucional da FURG (RI FURG) |
repository_id_str |
|
spelling |
Sodium uptake in different life stages of crustaceans: the water flea Daphnia magna StraussCrustaceanDaphnia magnaIon transportLife stageNa+ uptakeThe concentration-dependent kinetics and main mechanisms of whole-body Na+ uptake were assessed in neonate and adult water flea Daphnia magna Strauss acclimated to moderately hard water (0.6mmoll–1 NaCl, 1.0•mmol•l–1 CaCO3 and 0.15•mmol•l–1 MgSO4•7H2O; pH•8.2). Whole-body Na+ uptake is independent of the presence of Cl– in the external medium and kinetic parameters are dependent on the life stage. Adults have a lower maximum capacity of Na+ transport on a mass-specific basis but a higher affinity for Na+ when compared to neonates. Based on pharmacological analyses, mechanisms involved in whole-body Na+ uptake differ according to the life stage considered. In neonates, a proton pump-coupled Na+ channel appears to play an important role in the whole-body Na+ uptake at the apical membrane. However, they do not appear to contribute to whole-body Na+ uptake in adults, where only the Na+ channel seems to be present, associated with the Na+/H+ exchanger. In both cases, carbonic anhydrase contributes by providing H+ for the transporters. At the basolateral membrane of the salt-transporting epithelia of neonates, Na+ is pumped from the cells to the extracellular fluid by a Na+,K+-ATPase and a Na+/Cl– exchanger whereas K+ and Cl– move through specific channels. In adults, a Na+/K+/2Cl– cotransporter replaces the Na+/Cl– exchanger. Differential sensitivity of neonates and adults to iono- and osmoregulatory toxicants, such as metals, are discussed with respect to differences in whole-body Na+ uptake kinetics, as well as in the mechanisms of Na+ transport involved in the whole-body Na+ uptake in the two life stages.2011-06-17T04:13:51Z2011-06-17T04:13:51Z2008info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfBIANCHINI, Adalto; WOOD, Chris. Sodium uptake in different life stages of crustaceans: the water flea Daphnia magna StraussJournal of Experimental Biology, v. 211, p. 539-547, 2008. Disponível em:<http://jeb.biologists.org/content/211/4/539.full.pdf+html>. Acesso em: 13 jun. 2011.1477-9145http://repositorio.furg.br/handle/1/721engBianchini, AdaltoWood, Chrisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FURG (RI FURG)instname:Universidade Federal do Rio Grande (FURG)instacron:FURG2011-08-04T03:45:46Zoai:repositorio.furg.br:1/721Repositório InstitucionalPUBhttps://repositorio.furg.br/oai/request || http://200.19.254.174/oai/requestopendoar:2011-08-04T03:45:46Repositório Institucional da FURG (RI FURG) - Universidade Federal do Rio Grande (FURG)false |
dc.title.none.fl_str_mv |
Sodium uptake in different life stages of crustaceans: the water flea Daphnia magna Strauss |
title |
Sodium uptake in different life stages of crustaceans: the water flea Daphnia magna Strauss |
spellingShingle |
Sodium uptake in different life stages of crustaceans: the water flea Daphnia magna Strauss Bianchini, Adalto Crustacean Daphnia magna Ion transport Life stage Na+ uptake |
title_short |
Sodium uptake in different life stages of crustaceans: the water flea Daphnia magna Strauss |
title_full |
Sodium uptake in different life stages of crustaceans: the water flea Daphnia magna Strauss |
title_fullStr |
Sodium uptake in different life stages of crustaceans: the water flea Daphnia magna Strauss |
title_full_unstemmed |
Sodium uptake in different life stages of crustaceans: the water flea Daphnia magna Strauss |
title_sort |
Sodium uptake in different life stages of crustaceans: the water flea Daphnia magna Strauss |
author |
Bianchini, Adalto |
author_facet |
Bianchini, Adalto Wood, Chris |
author_role |
author |
author2 |
Wood, Chris |
author2_role |
author |
dc.contributor.author.fl_str_mv |
Bianchini, Adalto Wood, Chris |
dc.subject.por.fl_str_mv |
Crustacean Daphnia magna Ion transport Life stage Na+ uptake |
topic |
Crustacean Daphnia magna Ion transport Life stage Na+ uptake |
description |
The concentration-dependent kinetics and main mechanisms of whole-body Na+ uptake were assessed in neonate and adult water flea Daphnia magna Strauss acclimated to moderately hard water (0.6mmoll–1 NaCl, 1.0•mmol•l–1 CaCO3 and 0.15•mmol•l–1 MgSO4•7H2O; pH•8.2). Whole-body Na+ uptake is independent of the presence of Cl– in the external medium and kinetic parameters are dependent on the life stage. Adults have a lower maximum capacity of Na+ transport on a mass-specific basis but a higher affinity for Na+ when compared to neonates. Based on pharmacological analyses, mechanisms involved in whole-body Na+ uptake differ according to the life stage considered. In neonates, a proton pump-coupled Na+ channel appears to play an important role in the whole-body Na+ uptake at the apical membrane. However, they do not appear to contribute to whole-body Na+ uptake in adults, where only the Na+ channel seems to be present, associated with the Na+/H+ exchanger. In both cases, carbonic anhydrase contributes by providing H+ for the transporters. At the basolateral membrane of the salt-transporting epithelia of neonates, Na+ is pumped from the cells to the extracellular fluid by a Na+,K+-ATPase and a Na+/Cl– exchanger whereas K+ and Cl– move through specific channels. In adults, a Na+/K+/2Cl– cotransporter replaces the Na+/Cl– exchanger. Differential sensitivity of neonates and adults to iono- and osmoregulatory toxicants, such as metals, are discussed with respect to differences in whole-body Na+ uptake kinetics, as well as in the mechanisms of Na+ transport involved in the whole-body Na+ uptake in the two life stages. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008 2011-06-17T04:13:51Z 2011-06-17T04:13:51Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
BIANCHINI, Adalto; WOOD, Chris. Sodium uptake in different life stages of crustaceans: the water flea Daphnia magna StraussJournal of Experimental Biology, v. 211, p. 539-547, 2008. Disponível em:<http://jeb.biologists.org/content/211/4/539.full.pdf+html>. Acesso em: 13 jun. 2011. 1477-9145 http://repositorio.furg.br/handle/1/721 |
identifier_str_mv |
BIANCHINI, Adalto; WOOD, Chris. Sodium uptake in different life stages of crustaceans: the water flea Daphnia magna StraussJournal of Experimental Biology, v. 211, p. 539-547, 2008. Disponível em:<http://jeb.biologists.org/content/211/4/539.full.pdf+html>. Acesso em: 13 jun. 2011. 1477-9145 |
url |
http://repositorio.furg.br/handle/1/721 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da FURG (RI FURG) instname:Universidade Federal do Rio Grande (FURG) instacron:FURG |
instname_str |
Universidade Federal do Rio Grande (FURG) |
instacron_str |
FURG |
institution |
FURG |
reponame_str |
Repositório Institucional da FURG (RI FURG) |
collection |
Repositório Institucional da FURG (RI FURG) |
repository.name.fl_str_mv |
Repositório Institucional da FURG (RI FURG) - Universidade Federal do Rio Grande (FURG) |
repository.mail.fl_str_mv |
|
_version_ |
1807384417196310528 |