In vitro multiplication of Neoregelia johannis (Carriére) L. B. Smith in liquid medium under diferent concentrations of nutrients

Detalhes bibliográficos
Autor(a) principal: Meneghetti, Eveline Calderan
Data de Publicação: 2019
Outros Autores: de Oliveira, Leandro Silva, de Almeida, Marcilio
Tipo de documento: Artigo
Idioma: por
Título da fonte: Iheringia. Série Botânica
Texto Completo: https://isb.emnuvens.com.br/iheringia/article/view/814
Resumo: The importance of micropropagation to Bromeliaceae species is increasing, however few studies report the role of nutrition in in vitro multiplication. In order to correlate micropropagation morphophysiological aspects with regards to availability of nutrients, mainly nitrogen, Neoregelia johannis shoots were subcultured in the media culture MS, ½ MS and WPM, supplemented with the same concentrations of plant growth regulators. The multiplication rate was similar for all the culture media, with the highest multiplication rate, in addition to the highest accumulation of total and protein nitrogen, in the MS culture medium. Even producing the smallest shoots, the means of cultivation ½ MS and WPM represent a viable alternative for the maintenance of germplasm bank and for the maintenance of important genotypes in plant biofactories, considering that they present uniform results. The MS medium culture would be recommended for specific cases, when it is necessary to accelerate the multiplication and especially the growth of the shoots.
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spelling In vitro multiplication of Neoregelia johannis (Carriére) L. B. Smith in liquid medium under diferent concentrations of nutrientsMultiplicação in vitro de Neoregelia johannis (Carriére) L.B. Smith em meio líquido sob diferentes concentrações de nutrientesBromeliaceaemicropropagationnitrogenlandscaping.Bromeliaceaemicropropagaçãonitrogêniopaisagismo.Cultura de tecidosThe importance of micropropagation to Bromeliaceae species is increasing, however few studies report the role of nutrition in in vitro multiplication. In order to correlate micropropagation morphophysiological aspects with regards to availability of nutrients, mainly nitrogen, Neoregelia johannis shoots were subcultured in the media culture MS, ½ MS and WPM, supplemented with the same concentrations of plant growth regulators. The multiplication rate was similar for all the culture media, with the highest multiplication rate, in addition to the highest accumulation of total and protein nitrogen, in the MS culture medium. Even producing the smallest shoots, the means of cultivation ½ MS and WPM represent a viable alternative for the maintenance of germplasm bank and for the maintenance of important genotypes in plant biofactories, considering that they present uniform results. The MS medium culture would be recommended for specific cases, when it is necessary to accelerate the multiplication and especially the growth of the shoots.A importância da micropropagação para espécies da família Bromeliaceae é crescente, porém, poucos trabalhos relatam o papel da nutrição na multiplicação in vitro. Visando relacionar aspectos morfofisiológicos da micropropagação à disponibilidade de nutrientes, principalmente do nitrogênio, brotações de Neoregelia johannis foram subcultivadas nos meios de cultura MS, ½ MS e WPM, suplementados com as mesmas concentrações de reguladores de crescimento. A taxa de multiplicação foi semelhante nos três meios de cultura, sendo que a maior taxa de multiplicação, maior acúmulo de nitrogênio total e proteico ocorreu no meio de cultura MS. Mesmo produzindo brotações menores, o meio de cultura WPM representa uma alternativa viável para a manutenção de um banco de germoplasma e para a manutenção de genótipos de interesse em biofábricas, visto que apresenta desenvolvimento uniforme de brotações. O meio de cultura MS seria recomendado para casos específicos, quando é necessário acelerar a multiplicação e principalmente crescimento das brotações.Iheringia. Série Botânica.Iheringia, Série Botânica.2019-09-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://isb.emnuvens.com.br/iheringia/article/view/81410.21826/2446-82312019v74e2019012Iheringia. Série Botânica.; Vol. 74 (2019)Iheringia, Série Botânica.; v. 74 (2019)2446-82310073-4705reponame:Iheringia. Série Botânicainstname:Fundação Zoobotânica do Rio Grande do Sul (FZB/RS)instacron:FZB/RSporhttps://isb.emnuvens.com.br/iheringia/article/view/814/479Copyright (c) 2019 Iheringia. Série Botânica.info:eu-repo/semantics/openAccessMeneghetti, Eveline Calderande Oliveira, Leandro Silvade Almeida, Marcilio2019-10-16T12:35:13Zoai:ojs.emnuvens.com.br:article/814Revistahttps://isb.emnuvens.com.br/iheringiaONGhttps://isb.emnuvens.com.br/iheringia/oai||iheringia-bot@fzb.rs.gov.br2446-82310073-4705opendoar:2019-10-16T12:35:13Iheringia. Série Botânica - Fundação Zoobotânica do Rio Grande do Sul (FZB/RS)false
dc.title.none.fl_str_mv In vitro multiplication of Neoregelia johannis (Carriére) L. B. Smith in liquid medium under diferent concentrations of nutrients
Multiplicação in vitro de Neoregelia johannis (Carriére) L.B. Smith em meio líquido sob diferentes concentrações de nutrientes
title In vitro multiplication of Neoregelia johannis (Carriére) L. B. Smith in liquid medium under diferent concentrations of nutrients
spellingShingle In vitro multiplication of Neoregelia johannis (Carriére) L. B. Smith in liquid medium under diferent concentrations of nutrients
Meneghetti, Eveline Calderan
Bromeliaceae
micropropagation
nitrogen
landscaping.
Bromeliaceae
micropropagação
nitrogênio
paisagismo.
Cultura de tecidos
title_short In vitro multiplication of Neoregelia johannis (Carriére) L. B. Smith in liquid medium under diferent concentrations of nutrients
title_full In vitro multiplication of Neoregelia johannis (Carriére) L. B. Smith in liquid medium under diferent concentrations of nutrients
title_fullStr In vitro multiplication of Neoregelia johannis (Carriére) L. B. Smith in liquid medium under diferent concentrations of nutrients
title_full_unstemmed In vitro multiplication of Neoregelia johannis (Carriére) L. B. Smith in liquid medium under diferent concentrations of nutrients
title_sort In vitro multiplication of Neoregelia johannis (Carriére) L. B. Smith in liquid medium under diferent concentrations of nutrients
author Meneghetti, Eveline Calderan
author_facet Meneghetti, Eveline Calderan
de Oliveira, Leandro Silva
de Almeida, Marcilio
author_role author
author2 de Oliveira, Leandro Silva
de Almeida, Marcilio
author2_role author
author
dc.contributor.author.fl_str_mv Meneghetti, Eveline Calderan
de Oliveira, Leandro Silva
de Almeida, Marcilio
dc.subject.por.fl_str_mv Bromeliaceae
micropropagation
nitrogen
landscaping.
Bromeliaceae
micropropagação
nitrogênio
paisagismo.
Cultura de tecidos
topic Bromeliaceae
micropropagation
nitrogen
landscaping.
Bromeliaceae
micropropagação
nitrogênio
paisagismo.
Cultura de tecidos
description The importance of micropropagation to Bromeliaceae species is increasing, however few studies report the role of nutrition in in vitro multiplication. In order to correlate micropropagation morphophysiological aspects with regards to availability of nutrients, mainly nitrogen, Neoregelia johannis shoots were subcultured in the media culture MS, ½ MS and WPM, supplemented with the same concentrations of plant growth regulators. The multiplication rate was similar for all the culture media, with the highest multiplication rate, in addition to the highest accumulation of total and protein nitrogen, in the MS culture medium. Even producing the smallest shoots, the means of cultivation ½ MS and WPM represent a viable alternative for the maintenance of germplasm bank and for the maintenance of important genotypes in plant biofactories, considering that they present uniform results. The MS medium culture would be recommended for specific cases, when it is necessary to accelerate the multiplication and especially the growth of the shoots.
publishDate 2019
dc.date.none.fl_str_mv 2019-09-06
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://isb.emnuvens.com.br/iheringia/article/view/814
10.21826/2446-82312019v74e2019012
url https://isb.emnuvens.com.br/iheringia/article/view/814
identifier_str_mv 10.21826/2446-82312019v74e2019012
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://isb.emnuvens.com.br/iheringia/article/view/814/479
dc.rights.driver.fl_str_mv Copyright (c) 2019 Iheringia. Série Botânica.
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2019 Iheringia. Série Botânica.
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Iheringia. Série Botânica.
Iheringia, Série Botânica.
publisher.none.fl_str_mv Iheringia. Série Botânica.
Iheringia, Série Botânica.
dc.source.none.fl_str_mv Iheringia. Série Botânica.; Vol. 74 (2019)
Iheringia, Série Botânica.; v. 74 (2019)
2446-8231
0073-4705
reponame:Iheringia. Série Botânica
instname:Fundação Zoobotânica do Rio Grande do Sul (FZB/RS)
instacron:FZB/RS
instname_str Fundação Zoobotânica do Rio Grande do Sul (FZB/RS)
instacron_str FZB/RS
institution FZB/RS
reponame_str Iheringia. Série Botânica
collection Iheringia. Série Botânica
repository.name.fl_str_mv Iheringia. Série Botânica - Fundação Zoobotânica do Rio Grande do Sul (FZB/RS)
repository.mail.fl_str_mv ||iheringia-bot@fzb.rs.gov.br
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