Micropropagation of Dyckia agudensis Irgang & Sobral – an extinction threatened bromeliad

• Autor(a) principal: Silva, André Luís Lopes da
• Data de Publicação: 2007
• Outros Autores: Dornelles, Eduardo Bortoluzzi, Bisognin, Dilson Antônio, Franco, Elci Terezinha Henz, Horbach, Micheli Angélica
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Iheringia. Série Botânica
• Texto Completo: https://isb.emnuvens.com.br/iheringia/article/view/171
• topic: in vitro culture; bromeliaceae; direct organogenesis; hydroponics.; cultivo in vitro; bromeliaceae; organogênese direta; hidroponia.

Dyckia agudensis is an endemic species, that occurs on the Agudo hill, Agudo County (RS), it is considered threatened of extinction. The aim of this study was to develop a micropropagation protocol for this species to aid its conservation. The seeds were disinfected and germinated in vitro. Seedlings were submitted to the treatments of 0; 0.5; 1.0 or 2.0 mg.L-1 of BAP and IBA (same concentration) at 15 days of germination. The MS medium was supplemented with 2 ml.L-1 of Fuji vitamins, 30 g.L-1 of sucrose, and solidified with 6 g.L-1 of agar. After 60 days of cultivation, plantlets were transferred to a multiplication and regeneration medium. The medium was MS with 2 ml.L-1 of Fuji vitamins, 30 g.L-1 of sucrose, 0.5 mg.L-1 of BAP, and 7 g.L-1 of agar. The rooting medium was MS without growth regulators. The disinfection process obtained 85% of success. The same concentrations of BAP and IBA on the seedlings followed a quadratic regression, increasing shoot and bud numbers and swelling percentage, but reduced the percentage of rooting. The maximum efficacy was obtained close to 1.0 mg.L-1 of BAP and IBA in the culture medium. The m ultiplication phase proceeded together with the regeneration. The isolated shoots rooted without growth regulators. The acclimatization process was carried out in a hydroponic system for 15 days before transferring to the substrate.