Optimizing in vitro factors for improvement of shoot proliferation of ‘Flordaguard’ peach rootstock

Detalhes bibliográficos
Autor(a) principal: Bianchi, Valmor João
Data de Publicação: 2021
Outros Autores: Ritterbusch, Cristina Weiser, Lucho, Simone Ribeiro, Radmann, Elizete Beatriz
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Iheringia. Série Botânica
Texto Completo: https://isb.emnuvens.com.br/iheringia/article/view/952
Resumo: ‘Flordaguard’ has shown great potential as a standard rootstock for peach. The aim of this study was to examine the influence of Murashige & Skoog (MS) medium and modified MS-½N medium (half Nitrogen content), explant type and angle orientation on the culture medium, as well as 6-benzylaminopurine (BAP) concentrations on in vitro shoot proliferation in ‘Flordaguard’ rootstock. Explants cultured in MS-½N medium showed higher averages for number of lateral shoots and elongated shoots. The maximum average length of lateral shoots was obtained using doublephase medium. The 3.0 mg L-1 BAP induced the highest number of lateral shoots per explant (3.38 ±0.57). Whereas the higher number of lateral shoots elongated (1.75 ±0.10 cm) occurred at concentrations until 2.0 mg L-1 BAP. The study corroborated the importance of a 50% reduction in both NH4NO3 and KNO3 content in the cultured medium to improvein vitro propagation of ‘Flordaguard’ peach rootstock.
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spelling Optimizing in vitro factors for improvement of shoot proliferation of ‘Flordaguard’ peach rootstockBAPexplant orientation medium compositionPrunusCultivo in vitroBAP composição do meioPrunustipo do explante‘Flordaguard’ has shown great potential as a standard rootstock for peach. The aim of this study was to examine the influence of Murashige & Skoog (MS) medium and modified MS-½N medium (half Nitrogen content), explant type and angle orientation on the culture medium, as well as 6-benzylaminopurine (BAP) concentrations on in vitro shoot proliferation in ‘Flordaguard’ rootstock. Explants cultured in MS-½N medium showed higher averages for number of lateral shoots and elongated shoots. The maximum average length of lateral shoots was obtained using doublephase medium. The 3.0 mg L-1 BAP induced the highest number of lateral shoots per explant (3.38 ±0.57). Whereas the higher number of lateral shoots elongated (1.75 ±0.10 cm) occurred at concentrations until 2.0 mg L-1 BAP. The study corroborated the importance of a 50% reduction in both NH4NO3 and KNO3 content in the cultured medium to improvein vitro propagation of ‘Flordaguard’ peach rootstock.‘Flordaguard’ mostrou grande potencial para ser usada como porta-enxerto padrão para pessegueiro. O objetivo deste estudo foi examinar a influência da concentração do meio Murashige & Skoog (MS) e meio MS modificado (MS-½N - contendo metade da concentração de Nitrogênio), tipo de explante e ângulo de orientação do explante no meio de cultura, bem como de 6-benzilaminopurina (BAP) na proliferação de brotações in vitro no porta-enxerto ‘Flordaguard’. Explantes cultivados em meio MS-½N apresentaram as maiores médias para número de brotações laterais e de brotações alongadas. O maior comprimento médio de brotações laterais foi obtido usando meio dupla-fase. A concentração de 3,0 mg L-1 de BAP induziu o maior número médio de brotações laterais por explante (3,38 ± 0,57). Enquanto que o maior número de brotações alongadas (1,75 ± 0,10 cm) ocorreu em até 2,0 mg L-1 BAP. O estudo comprova a importância de reduzir em 50% as concentrações de NH4NO3 e KNO3 para a propagação in vitro do porta-enxerto ‘Flordaguard’.Iheringia. Série Botânica.Iheringia, Série Botânica.2021-06-21info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://isb.emnuvens.com.br/iheringia/article/view/95210.21826/2446-82312021v76e2021011Iheringia. Série Botânica.; Vol. 76 (2021)Iheringia, Série Botânica.; v. 76 (2021)2446-82310073-4705reponame:Iheringia. Série Botânicainstname:Fundação Zoobotânica do Rio Grande do Sul (FZB/RS)instacron:FZB/RSenghttps://isb.emnuvens.com.br/iheringia/article/view/952/525Copyright (c) 2021 Iheringia. Série Botânica.info:eu-repo/semantics/openAccessBianchi, Valmor JoãoRitterbusch, Cristina WeiserLucho, Simone RibeiroRadmann, Elizete Beatriz2021-06-21T13:45:46Zoai:ojs.emnuvens.com.br:article/952Revistahttps://isb.emnuvens.com.br/iheringiaONGhttps://isb.emnuvens.com.br/iheringia/oai||iheringia-bot@fzb.rs.gov.br2446-82310073-4705opendoar:2021-06-21T13:45:46Iheringia. Série Botânica - Fundação Zoobotânica do Rio Grande do Sul (FZB/RS)false
dc.title.none.fl_str_mv Optimizing in vitro factors for improvement of shoot proliferation of ‘Flordaguard’ peach rootstock
title Optimizing in vitro factors for improvement of shoot proliferation of ‘Flordaguard’ peach rootstock
spellingShingle Optimizing in vitro factors for improvement of shoot proliferation of ‘Flordaguard’ peach rootstock
Bianchi, Valmor João
BAP
explant orientation
medium composition
Prunus
Cultivo in vitro
BAP
composição do meio
Prunus
tipo do explante
title_short Optimizing in vitro factors for improvement of shoot proliferation of ‘Flordaguard’ peach rootstock
title_full Optimizing in vitro factors for improvement of shoot proliferation of ‘Flordaguard’ peach rootstock
title_fullStr Optimizing in vitro factors for improvement of shoot proliferation of ‘Flordaguard’ peach rootstock
title_full_unstemmed Optimizing in vitro factors for improvement of shoot proliferation of ‘Flordaguard’ peach rootstock
title_sort Optimizing in vitro factors for improvement of shoot proliferation of ‘Flordaguard’ peach rootstock
author Bianchi, Valmor João
author_facet Bianchi, Valmor João
Ritterbusch, Cristina Weiser
Lucho, Simone Ribeiro
Radmann, Elizete Beatriz
author_role author
author2 Ritterbusch, Cristina Weiser
Lucho, Simone Ribeiro
Radmann, Elizete Beatriz
author2_role author
author
author
dc.contributor.author.fl_str_mv Bianchi, Valmor João
Ritterbusch, Cristina Weiser
Lucho, Simone Ribeiro
Radmann, Elizete Beatriz
dc.subject.por.fl_str_mv BAP
explant orientation
medium composition
Prunus
Cultivo in vitro
BAP
composição do meio
Prunus
tipo do explante
topic BAP
explant orientation
medium composition
Prunus
Cultivo in vitro
BAP
composição do meio
Prunus
tipo do explante
description ‘Flordaguard’ has shown great potential as a standard rootstock for peach. The aim of this study was to examine the influence of Murashige & Skoog (MS) medium and modified MS-½N medium (half Nitrogen content), explant type and angle orientation on the culture medium, as well as 6-benzylaminopurine (BAP) concentrations on in vitro shoot proliferation in ‘Flordaguard’ rootstock. Explants cultured in MS-½N medium showed higher averages for number of lateral shoots and elongated shoots. The maximum average length of lateral shoots was obtained using doublephase medium. The 3.0 mg L-1 BAP induced the highest number of lateral shoots per explant (3.38 ±0.57). Whereas the higher number of lateral shoots elongated (1.75 ±0.10 cm) occurred at concentrations until 2.0 mg L-1 BAP. The study corroborated the importance of a 50% reduction in both NH4NO3 and KNO3 content in the cultured medium to improvein vitro propagation of ‘Flordaguard’ peach rootstock.
publishDate 2021
dc.date.none.fl_str_mv 2021-06-21
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://isb.emnuvens.com.br/iheringia/article/view/952
10.21826/2446-82312021v76e2021011
url https://isb.emnuvens.com.br/iheringia/article/view/952
identifier_str_mv 10.21826/2446-82312021v76e2021011
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://isb.emnuvens.com.br/iheringia/article/view/952/525
dc.rights.driver.fl_str_mv Copyright (c) 2021 Iheringia. Série Botânica.
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2021 Iheringia. Série Botânica.
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Iheringia. Série Botânica.
Iheringia, Série Botânica.
publisher.none.fl_str_mv Iheringia. Série Botânica.
Iheringia, Série Botânica.
dc.source.none.fl_str_mv Iheringia. Série Botânica.; Vol. 76 (2021)
Iheringia, Série Botânica.; v. 76 (2021)
2446-8231
0073-4705
reponame:Iheringia. Série Botânica
instname:Fundação Zoobotânica do Rio Grande do Sul (FZB/RS)
instacron:FZB/RS
instname_str Fundação Zoobotânica do Rio Grande do Sul (FZB/RS)
instacron_str FZB/RS
institution FZB/RS
reponame_str Iheringia. Série Botânica
collection Iheringia. Série Botânica
repository.name.fl_str_mv Iheringia. Série Botânica - Fundação Zoobotânica do Rio Grande do Sul (FZB/RS)
repository.mail.fl_str_mv ||iheringia-bot@fzb.rs.gov.br
_version_ 1798948136451833856

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