What is the influence of oxygen in high concentration in the hyperbaric chamber on bone metabolism?
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2020 |
| Outros Autores: | , |
| Tipo de documento: | Artigo |
| Idioma: | por |
| Título da fonte: | Brazilian Journal of Implantology and Health Sciences |
| Texto Completo: | https://bjihs.emnuvens.com.br/bjihs/article/view/127 |
Resumo: | Objectives: To know the actions of oxygen in high concentration in the hyperbaric chamber (HC) in the expression of genes related to bone metabolism in osteoblastic cell lines and human trabecular bone. Material and methods: The differential expression of several genes related to bone metabolism (SOST, RUNX2, MMP14, OPG, HIF-1α and SIRT1) was analyzed in two cell lines of human osteoblasts (Saos and Super-Saos) and in bone fragments human trabecular submitted to one, three or five sessions of CH (90 minutes, 100% oxygen; 2.3 atmospheres). A control that did not receive CH was used in each experiment. Results: We found no significant differences after CH in the expression of the studied genes, neither in cells nor in trabecular bone. Only in the Super-Saos strain did OPG expression decrease after 5 CH sessions 6 times compared to the control group (2 -ΔCt of 72; p = 0.01). Conclusions: Oxygen in high concentrations in the hyperbaric chamber does not appear to influence the expression of genes related to bone metabolism. |
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Brazilian Journal of Implantology and Health Sciences |
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What is the influence of oxygen in high concentration in the hyperbaric chamber on bone metabolism?Qual a influência do oxigênio em alta concentração na câmara hiperbárica no metabolismo ósseo? genesossoCâmara hiperbáricaOxigênioOxygen; Hyperbaric chamber; bone; genes.Objectives: To know the actions of oxygen in high concentration in the hyperbaric chamber (HC) in the expression of genes related to bone metabolism in osteoblastic cell lines and human trabecular bone. Material and methods: The differential expression of several genes related to bone metabolism (SOST, RUNX2, MMP14, OPG, HIF-1α and SIRT1) was analyzed in two cell lines of human osteoblasts (Saos and Super-Saos) and in bone fragments human trabecular submitted to one, three or five sessions of CH (90 minutes, 100% oxygen; 2.3 atmospheres). A control that did not receive CH was used in each experiment. Results: We found no significant differences after CH in the expression of the studied genes, neither in cells nor in trabecular bone. Only in the Super-Saos strain did OPG expression decrease after 5 CH sessions 6 times compared to the control group (2 -ΔCt of 72; p = 0.01). Conclusions: Oxygen in high concentrations in the hyperbaric chamber does not appear to influence the expression of genes related to bone metabolism.Objetivos: Conhecer as ações do oxigênio em alta concentração na câmara hiperbárica (HC) na expressão de genes relacionados ao metabolismo ósseo em linhagens de células osteoblásticas e osso trabecular humano. Material e métodos: A expressão diferencial de vários genes relacionados ao metabolismo ósseo (SOST, RUNX2, MMP14, OPG, HIF-1α e SIRT1) foi analisada em duas linhagens celulares de osteoblastos humanos (Saos e Super-Saos) e em fragmentos de osso trabecular humano submetidos a uma, três ou cinco sessões de CH (90 minutos, oxigênio a 100%; 2,3 atmosferas). Um controle que não recebeu CH foi usado em cada experimento. Resultados: Não encontramos diferenças significativas após o CH na expressão dos genes estudados, nem nas células nem no osso trabecular. Apenas na linhagem Super-Saos a expressão de OPG após 5 sessões de CH diminuiu 6 vezes em relação ao grupo controle (2 -ΔCt de 72; p = 0,01). Conclusões: O oxigênio em altas concentrações na câmara hiperbárica não parece influenciar a expressão de genes relacionados ao metabolismo ósseo.Specialized Dentistry Group2020-10-29info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://bjihs.emnuvens.com.br/bjihs/article/view/12710.36557/2674-8169.2020v2n11p24-34Brazilian Journal of Implantology and Health Sciences ; Vol. 2 No. 11 (2020): October; 24-34Brazilian Journal of Implantology and Health Sciences ; Vol. 2 Núm. 11 (2020): Outubro; 24-34Brazilian Journal of Implantology and Health Sciences ; v. 2 n. 11 (2020): Outubro; 24-342674-8169reponame:Brazilian Journal of Implantology and Health Sciencesinstname:Grupo de Odontologia Especializada (GOE)instacron:GOEporhttps://bjihs.emnuvens.com.br/bjihs/article/view/127/178Copyright (c) 2020 João Cassio Cunha , Antonio Marcos Realis Rossi , Maicon Ribeiro de Maraishttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessCunha , João Cassio Realis Rossi , Antonio Marcos Ribeiro de Marais, Maicon 2020-11-22T21:31:09Zoai:ojs.bjihs.emnuvens.com.br:article/127Revistahttps://bjihs.emnuvens.com.br/bjihsONGhttps://bjihs.emnuvens.com.br/bjihs/oaijournal.bjihs@periodicosbrasil.com.br2674-81692674-8169opendoar:2020-11-22T21:31:09Brazilian Journal of Implantology and Health Sciences - Grupo de Odontologia Especializada (GOE)false |
| dc.title.none.fl_str_mv |
What is the influence of oxygen in high concentration in the hyperbaric chamber on bone metabolism? Qual a influência do oxigênio em alta concentração na câmara hiperbárica no metabolismo ósseo? |
| title |
What is the influence of oxygen in high concentration in the hyperbaric chamber on bone metabolism? |
| spellingShingle |
What is the influence of oxygen in high concentration in the hyperbaric chamber on bone metabolism? Cunha , João Cassio genes osso Câmara hiperbárica Oxigênio Oxygen; Hyperbaric chamber; bone; genes. |
| title_short |
What is the influence of oxygen in high concentration in the hyperbaric chamber on bone metabolism? |
| title_full |
What is the influence of oxygen in high concentration in the hyperbaric chamber on bone metabolism? |
| title_fullStr |
What is the influence of oxygen in high concentration in the hyperbaric chamber on bone metabolism? |
| title_full_unstemmed |
What is the influence of oxygen in high concentration in the hyperbaric chamber on bone metabolism? |
| title_sort |
What is the influence of oxygen in high concentration in the hyperbaric chamber on bone metabolism? |
| author |
Cunha , João Cassio |
| author_facet |
Cunha , João Cassio Realis Rossi , Antonio Marcos Ribeiro de Marais, Maicon |
| author_role |
author |
| author2 |
Realis Rossi , Antonio Marcos Ribeiro de Marais, Maicon |
| author2_role |
author author |
| dc.contributor.author.fl_str_mv |
Cunha , João Cassio Realis Rossi , Antonio Marcos Ribeiro de Marais, Maicon |
| dc.subject.por.fl_str_mv |
genes osso Câmara hiperbárica Oxigênio Oxygen; Hyperbaric chamber; bone; genes. |
| topic |
genes osso Câmara hiperbárica Oxigênio Oxygen; Hyperbaric chamber; bone; genes. |
| description |
Objectives: To know the actions of oxygen in high concentration in the hyperbaric chamber (HC) in the expression of genes related to bone metabolism in osteoblastic cell lines and human trabecular bone. Material and methods: The differential expression of several genes related to bone metabolism (SOST, RUNX2, MMP14, OPG, HIF-1α and SIRT1) was analyzed in two cell lines of human osteoblasts (Saos and Super-Saos) and in bone fragments human trabecular submitted to one, three or five sessions of CH (90 minutes, 100% oxygen; 2.3 atmospheres). A control that did not receive CH was used in each experiment. Results: We found no significant differences after CH in the expression of the studied genes, neither in cells nor in trabecular bone. Only in the Super-Saos strain did OPG expression decrease after 5 CH sessions 6 times compared to the control group (2 -ΔCt of 72; p = 0.01). Conclusions: Oxygen in high concentrations in the hyperbaric chamber does not appear to influence the expression of genes related to bone metabolism. |
| publishDate |
2020 |
| dc.date.none.fl_str_mv |
2020-10-29 |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
https://bjihs.emnuvens.com.br/bjihs/article/view/127 10.36557/2674-8169.2020v2n11p24-34 |
| url |
https://bjihs.emnuvens.com.br/bjihs/article/view/127 |
| identifier_str_mv |
10.36557/2674-8169.2020v2n11p24-34 |
| dc.language.iso.fl_str_mv |
por |
| language |
por |
| dc.relation.none.fl_str_mv |
https://bjihs.emnuvens.com.br/bjihs/article/view/127/178 |
| dc.rights.driver.fl_str_mv |
Copyright (c) 2020 João Cassio Cunha , Antonio Marcos Realis Rossi , Maicon Ribeiro de Marais https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
Copyright (c) 2020 João Cassio Cunha , Antonio Marcos Realis Rossi , Maicon Ribeiro de Marais https://creativecommons.org/licenses/by/4.0 |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
Specialized Dentistry Group |
| publisher.none.fl_str_mv |
Specialized Dentistry Group |
| dc.source.none.fl_str_mv |
Brazilian Journal of Implantology and Health Sciences ; Vol. 2 No. 11 (2020): October; 24-34 Brazilian Journal of Implantology and Health Sciences ; Vol. 2 Núm. 11 (2020): Outubro; 24-34 Brazilian Journal of Implantology and Health Sciences ; v. 2 n. 11 (2020): Outubro; 24-34 2674-8169 reponame:Brazilian Journal of Implantology and Health Sciences instname:Grupo de Odontologia Especializada (GOE) instacron:GOE |
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Grupo de Odontologia Especializada (GOE) |
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GOE |
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GOE |
| reponame_str |
Brazilian Journal of Implantology and Health Sciences |
| collection |
Brazilian Journal of Implantology and Health Sciences |
| repository.name.fl_str_mv |
Brazilian Journal of Implantology and Health Sciences - Grupo de Odontologia Especializada (GOE) |
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journal.bjihs@periodicosbrasil.com.br |
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1796798448312254464 |