Reaction of detached leaves from different varieties of sweet orange to inoculation with Xanthomonas citri subsp. citri
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Summa phytopathologica (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-54052016000200125 |
Resumo: | ABSTRACT Citrus genotypes show a broad range of resistance to infection with Xanthomonas citri subsp citri (Xcc), the causal agent of Asiatic citrus canker (ACC). The aim of this study was to evaluate a detached leaf inoculation method to compare nine different citrus genotypes that exhibit varied resistance levels to ACC, and to evaluate infection, colonization and symptom development of the varieties by using Scanning Electron Microscopy (SEM). Inoculation of Xcc in the leaves was carried out by producing injuries with needles. Lesion diameters were measured at 7, 10 and 13 days after inoculation (DAI). The variety Pera IAC was most resistant to the pathogen and had lesions with smaller diameters, while the variety Washington was most susceptible and had the largest lesions, indicating low resistance to Xcc. SEM images were taken from the lesion development, and sections were obtained from the disease lesions in the tested varieties. The most resistant variety, Pera IAC, had smaller stomatal openings and produced greater quantity of foliar exudates, compared with the variety Washington. We hypothesize that the exudates may contain Xcc toxic compounds and thus may be a defense mechanism against infection by Xcc, but further research is required to establish any antibiotic effects of the exudates against citrus canker. |
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Reaction of detached leaves from different varieties of sweet orange to inoculation with Xanthomonas citri subsp. citriCitrus cankerInoculationScanning Electron Microscopy (SEM)resistanceABSTRACT Citrus genotypes show a broad range of resistance to infection with Xanthomonas citri subsp citri (Xcc), the causal agent of Asiatic citrus canker (ACC). The aim of this study was to evaluate a detached leaf inoculation method to compare nine different citrus genotypes that exhibit varied resistance levels to ACC, and to evaluate infection, colonization and symptom development of the varieties by using Scanning Electron Microscopy (SEM). Inoculation of Xcc in the leaves was carried out by producing injuries with needles. Lesion diameters were measured at 7, 10 and 13 days after inoculation (DAI). The variety Pera IAC was most resistant to the pathogen and had lesions with smaller diameters, while the variety Washington was most susceptible and had the largest lesions, indicating low resistance to Xcc. SEM images were taken from the lesion development, and sections were obtained from the disease lesions in the tested varieties. The most resistant variety, Pera IAC, had smaller stomatal openings and produced greater quantity of foliar exudates, compared with the variety Washington. We hypothesize that the exudates may contain Xcc toxic compounds and thus may be a defense mechanism against infection by Xcc, but further research is required to establish any antibiotic effects of the exudates against citrus canker.Grupo Paulista de Fitopatologia2016-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-54052016000200125Summa Phytopathologica v.42 n.2 2016reponame:Summa phytopathologica (Online)instname:Grupo Paulista de Fitopatologiainstacron:GPF10.1590/0100-5405/2143info:eu-repo/semantics/openAccessGonçalves-Zuliani,Aline Maria OrbolatoCardoso,Kátia Aparecida KernBelasque Junior,JoséZanutto,Carlos AlexandreHashiguti,Heraldo TakaoBock,Clive HowardNakamura,Celso VataruNunes,William Mário de Carvalhoeng2016-06-21T00:00:00Zoai:scielo:S0100-54052016000200125Revistahttp://www.scielo.br/sphttps://old.scielo.br/oai/scielo-oai.phpsumma@fca.unesp.br1980-54540100-5405opendoar:2016-06-21T00:00Summa phytopathologica (Online) - Grupo Paulista de Fitopatologiafalse |
dc.title.none.fl_str_mv |
Reaction of detached leaves from different varieties of sweet orange to inoculation with Xanthomonas citri subsp. citri |
title |
Reaction of detached leaves from different varieties of sweet orange to inoculation with Xanthomonas citri subsp. citri |
spellingShingle |
Reaction of detached leaves from different varieties of sweet orange to inoculation with Xanthomonas citri subsp. citri Gonçalves-Zuliani,Aline Maria Orbolato Citrus canker Inoculation Scanning Electron Microscopy (SEM) resistance |
title_short |
Reaction of detached leaves from different varieties of sweet orange to inoculation with Xanthomonas citri subsp. citri |
title_full |
Reaction of detached leaves from different varieties of sweet orange to inoculation with Xanthomonas citri subsp. citri |
title_fullStr |
Reaction of detached leaves from different varieties of sweet orange to inoculation with Xanthomonas citri subsp. citri |
title_full_unstemmed |
Reaction of detached leaves from different varieties of sweet orange to inoculation with Xanthomonas citri subsp. citri |
title_sort |
Reaction of detached leaves from different varieties of sweet orange to inoculation with Xanthomonas citri subsp. citri |
author |
Gonçalves-Zuliani,Aline Maria Orbolato |
author_facet |
Gonçalves-Zuliani,Aline Maria Orbolato Cardoso,Kátia Aparecida Kern Belasque Junior,José Zanutto,Carlos Alexandre Hashiguti,Heraldo Takao Bock,Clive Howard Nakamura,Celso Vataru Nunes,William Mário de Carvalho |
author_role |
author |
author2 |
Cardoso,Kátia Aparecida Kern Belasque Junior,José Zanutto,Carlos Alexandre Hashiguti,Heraldo Takao Bock,Clive Howard Nakamura,Celso Vataru Nunes,William Mário de Carvalho |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Gonçalves-Zuliani,Aline Maria Orbolato Cardoso,Kátia Aparecida Kern Belasque Junior,José Zanutto,Carlos Alexandre Hashiguti,Heraldo Takao Bock,Clive Howard Nakamura,Celso Vataru Nunes,William Mário de Carvalho |
dc.subject.por.fl_str_mv |
Citrus canker Inoculation Scanning Electron Microscopy (SEM) resistance |
topic |
Citrus canker Inoculation Scanning Electron Microscopy (SEM) resistance |
description |
ABSTRACT Citrus genotypes show a broad range of resistance to infection with Xanthomonas citri subsp citri (Xcc), the causal agent of Asiatic citrus canker (ACC). The aim of this study was to evaluate a detached leaf inoculation method to compare nine different citrus genotypes that exhibit varied resistance levels to ACC, and to evaluate infection, colonization and symptom development of the varieties by using Scanning Electron Microscopy (SEM). Inoculation of Xcc in the leaves was carried out by producing injuries with needles. Lesion diameters were measured at 7, 10 and 13 days after inoculation (DAI). The variety Pera IAC was most resistant to the pathogen and had lesions with smaller diameters, while the variety Washington was most susceptible and had the largest lesions, indicating low resistance to Xcc. SEM images were taken from the lesion development, and sections were obtained from the disease lesions in the tested varieties. The most resistant variety, Pera IAC, had smaller stomatal openings and produced greater quantity of foliar exudates, compared with the variety Washington. We hypothesize that the exudates may contain Xcc toxic compounds and thus may be a defense mechanism against infection by Xcc, but further research is required to establish any antibiotic effects of the exudates against citrus canker. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-54052016000200125 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-54052016000200125 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0100-5405/2143 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Grupo Paulista de Fitopatologia |
publisher.none.fl_str_mv |
Grupo Paulista de Fitopatologia |
dc.source.none.fl_str_mv |
Summa Phytopathologica v.42 n.2 2016 reponame:Summa phytopathologica (Online) instname:Grupo Paulista de Fitopatologia instacron:GPF |
instname_str |
Grupo Paulista de Fitopatologia |
instacron_str |
GPF |
institution |
GPF |
reponame_str |
Summa phytopathologica (Online) |
collection |
Summa phytopathologica (Online) |
repository.name.fl_str_mv |
Summa phytopathologica (Online) - Grupo Paulista de Fitopatologia |
repository.mail.fl_str_mv |
summa@fca.unesp.br |
_version_ |
1754193418466099200 |