Preparation of colubrine seed for tetrazolium test

• Autor(a) principal: Fernandes Moraes, Pablo Henrique
• Data de Publicação: 2019
• Outros Autores: Cavalcante, Laura Veríssimo, Albuquerque, Asclépio Silva de, Rodrigues, Amanda Maria Carlos, Andrade Melo, Luan Danilo Ferreira de, Melo Junior, João Luciano de Andrade
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Revista Verde de Agroecologia e Desenvolvimento Sustentavel
• Texto Completo: https://www.gvaa.com.br/revista/index.php/RVADS/article/view/7599
• topic: Caatinga; Colubrina glandulosa; Staining periods; Viable seeds; Colubrina glandulosa; Períodos de coloración; Viabilidad de semillas; Caatinga; Colubrina glandulosa; Períodos de coloração; Viabilidade de sementes

The Caatinga ecoregion has a great diversity of species with exploitation potential. Among them, colubrina (Colubrina glandulosa Perkins) stands out for its importance in logging and forestry activities. Its seeds have low, slow and uneven germination. Knowing that the use of the tetrazolium test to estimate viability becomes essential when rapid response on seed quality is desired, this study aimed to establish the best tetrazolium solution concentration and staining period to evaluate the viability of Colubrina glandulosa seeds. Initially, the seeds were previously moistened between sheets of paper for five hours at 30 ºC. Subsequently, the portion of the cotyledon containing the embryo was immersed in four tetrazolium solution concentrations (0.075, 0.1, 0.5, and 1.0%) and three staining periods (2, 4, and 6 hours) in the dark. under the temperature of 30 ºC. The percentage of viable seeds was compared with the results obtained in the germination test performed on the paper substrate at 30 ºC in four replications of 25 seeds. The tetrazolium test was efficient to estimate the viability of C. glandulosa seeds, and the concentration of 0.075% for four hours at 30 ºC was the best condition.