Symbiotic and agronomic efficiency of new cowpea rhizobia from Brazilian Semi-Arid

Detalhes bibliográficos
Autor(a) principal: Marinho,Rita de Cássia Nunes
Data de Publicação: 2017
Outros Autores: Ferreira,Linnajara de Vasconcelos Martins, Silva,Aleksandro Ferreira da, Martins,Lindete Míria Vieira, Nóbrega,Rafaela Simão Abrahão, Fernandes-Júnior,Paulo Ivan
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Bragantia
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052017000200273
Resumo: ABSTRACT Cowpea is a very important crop to Brazilian Semi-Arid mainly small family-based farmers. Rhizobia inoculation is a practice, easy to use, and cheap technology that increases cowpea productivity. The aim of this study was to evaluate the efficiency of two new rhizobia isolates in greenhouse and field as well as classify them taxonomically. To bacterial identification the 16S rRNA gene of ESA 17 and ESA 18 isolates were sequenced. The greenhouse test was conducted with pots containing 3 L of soil and the bacterial isolates evaluated were ESA 17, ESA 18, BR 3267 or BR 3262 strains. A field experiment was implemented on a Vertisol in Juazeiro, Bahia State, to evaluate the cowpea growth and productivity. In this experiment, the peat-based inoculants with ESA 17, ESA 18, BR 3267 or UFLA 3-84 were used in 2 cowpea cultivars. Both bacteria were identified as Bradyrhizobium, but related to different species. ESA 17 was related to B. japonicum and ESA 18 was closer to B. pachyrhizi. At greenhouse, both isolates increased cowpea nitrogen content in the shoots due to the presence of very efficient nodules. In the field, the isolate ESA 18 inoculated at BRS Pujante cultivar induced higher production than observed for the absolute control, and for BR 17 Gurguéia cultivar, the ESA 17 and BR 3267 stood out both by inducing high production and grain protein content. The results indicate that both isolates can be evaluated in network experiments aiming at official recommendation for new bacteria to cowpea inoculant in Brazil.
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spelling Symbiotic and agronomic efficiency of new cowpea rhizobia from Brazilian Semi-AridBradyrhizobiuminoculantbiological nitrogen fixationstrain selectionSão Francisco River ValleyABSTRACT Cowpea is a very important crop to Brazilian Semi-Arid mainly small family-based farmers. Rhizobia inoculation is a practice, easy to use, and cheap technology that increases cowpea productivity. The aim of this study was to evaluate the efficiency of two new rhizobia isolates in greenhouse and field as well as classify them taxonomically. To bacterial identification the 16S rRNA gene of ESA 17 and ESA 18 isolates were sequenced. The greenhouse test was conducted with pots containing 3 L of soil and the bacterial isolates evaluated were ESA 17, ESA 18, BR 3267 or BR 3262 strains. A field experiment was implemented on a Vertisol in Juazeiro, Bahia State, to evaluate the cowpea growth and productivity. In this experiment, the peat-based inoculants with ESA 17, ESA 18, BR 3267 or UFLA 3-84 were used in 2 cowpea cultivars. Both bacteria were identified as Bradyrhizobium, but related to different species. ESA 17 was related to B. japonicum and ESA 18 was closer to B. pachyrhizi. At greenhouse, both isolates increased cowpea nitrogen content in the shoots due to the presence of very efficient nodules. In the field, the isolate ESA 18 inoculated at BRS Pujante cultivar induced higher production than observed for the absolute control, and for BR 17 Gurguéia cultivar, the ESA 17 and BR 3267 stood out both by inducing high production and grain protein content. The results indicate that both isolates can be evaluated in network experiments aiming at official recommendation for new bacteria to cowpea inoculant in Brazil.Instituto Agronômico de Campinas2017-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052017000200273Bragantia v.76 n.2 2017reponame:Bragantiainstname:Instituto Agronômico de Campinas (IAC)instacron:IAC10.1590/1678-4499.003info:eu-repo/semantics/openAccessMarinho,Rita de Cássia NunesFerreira,Linnajara de Vasconcelos MartinsSilva,Aleksandro Ferreira daMartins,Lindete Míria VieiraNóbrega,Rafaela Simão AbrahãoFernandes-Júnior,Paulo Ivaneng2017-05-11T00:00:00Zoai:scielo:S0006-87052017000200273Revistahttps://www.scielo.br/j/brag/https://old.scielo.br/oai/scielo-oai.phpbragantia@iac.sp.gov.br||bragantia@iac.sp.gov.br1678-44990006-8705opendoar:2017-05-11T00:00Bragantia - Instituto Agronômico de Campinas (IAC)false
dc.title.none.fl_str_mv Symbiotic and agronomic efficiency of new cowpea rhizobia from Brazilian Semi-Arid
title Symbiotic and agronomic efficiency of new cowpea rhizobia from Brazilian Semi-Arid
spellingShingle Symbiotic and agronomic efficiency of new cowpea rhizobia from Brazilian Semi-Arid
Marinho,Rita de Cássia Nunes
Bradyrhizobium
inoculant
biological nitrogen fixation
strain selection
São Francisco River Valley
title_short Symbiotic and agronomic efficiency of new cowpea rhizobia from Brazilian Semi-Arid
title_full Symbiotic and agronomic efficiency of new cowpea rhizobia from Brazilian Semi-Arid
title_fullStr Symbiotic and agronomic efficiency of new cowpea rhizobia from Brazilian Semi-Arid
title_full_unstemmed Symbiotic and agronomic efficiency of new cowpea rhizobia from Brazilian Semi-Arid
title_sort Symbiotic and agronomic efficiency of new cowpea rhizobia from Brazilian Semi-Arid
author Marinho,Rita de Cássia Nunes
author_facet Marinho,Rita de Cássia Nunes
Ferreira,Linnajara de Vasconcelos Martins
Silva,Aleksandro Ferreira da
Martins,Lindete Míria Vieira
Nóbrega,Rafaela Simão Abrahão
Fernandes-Júnior,Paulo Ivan
author_role author
author2 Ferreira,Linnajara de Vasconcelos Martins
Silva,Aleksandro Ferreira da
Martins,Lindete Míria Vieira
Nóbrega,Rafaela Simão Abrahão
Fernandes-Júnior,Paulo Ivan
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Marinho,Rita de Cássia Nunes
Ferreira,Linnajara de Vasconcelos Martins
Silva,Aleksandro Ferreira da
Martins,Lindete Míria Vieira
Nóbrega,Rafaela Simão Abrahão
Fernandes-Júnior,Paulo Ivan
dc.subject.por.fl_str_mv Bradyrhizobium
inoculant
biological nitrogen fixation
strain selection
São Francisco River Valley
topic Bradyrhizobium
inoculant
biological nitrogen fixation
strain selection
São Francisco River Valley
description ABSTRACT Cowpea is a very important crop to Brazilian Semi-Arid mainly small family-based farmers. Rhizobia inoculation is a practice, easy to use, and cheap technology that increases cowpea productivity. The aim of this study was to evaluate the efficiency of two new rhizobia isolates in greenhouse and field as well as classify them taxonomically. To bacterial identification the 16S rRNA gene of ESA 17 and ESA 18 isolates were sequenced. The greenhouse test was conducted with pots containing 3 L of soil and the bacterial isolates evaluated were ESA 17, ESA 18, BR 3267 or BR 3262 strains. A field experiment was implemented on a Vertisol in Juazeiro, Bahia State, to evaluate the cowpea growth and productivity. In this experiment, the peat-based inoculants with ESA 17, ESA 18, BR 3267 or UFLA 3-84 were used in 2 cowpea cultivars. Both bacteria were identified as Bradyrhizobium, but related to different species. ESA 17 was related to B. japonicum and ESA 18 was closer to B. pachyrhizi. At greenhouse, both isolates increased cowpea nitrogen content in the shoots due to the presence of very efficient nodules. In the field, the isolate ESA 18 inoculated at BRS Pujante cultivar induced higher production than observed for the absolute control, and for BR 17 Gurguéia cultivar, the ESA 17 and BR 3267 stood out both by inducing high production and grain protein content. The results indicate that both isolates can be evaluated in network experiments aiming at official recommendation for new bacteria to cowpea inoculant in Brazil.
publishDate 2017
dc.date.none.fl_str_mv 2017-04-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052017000200273
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052017000200273
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1678-4499.003
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Agronômico de Campinas
publisher.none.fl_str_mv Instituto Agronômico de Campinas
dc.source.none.fl_str_mv Bragantia v.76 n.2 2017
reponame:Bragantia
instname:Instituto Agronômico de Campinas (IAC)
instacron:IAC
instname_str Instituto Agronômico de Campinas (IAC)
instacron_str IAC
institution IAC
reponame_str Bragantia
collection Bragantia
repository.name.fl_str_mv Bragantia - Instituto Agronômico de Campinas (IAC)
repository.mail.fl_str_mv bragantia@iac.sp.gov.br||bragantia@iac.sp.gov.br
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