Effects of aluminum on the elongation and external morphology of root tips in two maize genotypes
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Bragantia |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052016000100019 |
Resumo: | ABSTRACT The objective of this study was to evaluate the effects of toxic levels of aluminum (Al) on the growth and external morphology of root tips in two maize genotypes with differential Al tolerance. The maize genotypes UFVM-100 (Al-sensitive) and UFVM-200 (Al-tolerant) were treated with 0 and 50 mM Al in a 0.5 mM CaCl2 solution at pH 4.5; root elongation, Al content and the external morphology of the root tips were evaluated. Chemical analysis, hematoxylin staining and energy dispersive X-ray spectrometry (EDS) showed greater Al accumulation in the root tips of the UFVM-200 genotype. The inhibition of root elongation in the UFVM-100 genotype, however, was much stronger than in the UFVM-200 genotype. Both maize genotypes exhibited visible and intense alterations in external micromorphology of the root tip, especially the Al-sensitive UFVM-100 genotype. Scanning electron micrographs showed intense cell disorganization and transverse ruptures of the protodermic and outer cortex layers of the cells in both genotypes. The ruptures were deeper and wider and reached the inner cortex layers in the UFVM-100 genotype. The EDS analysis showed that, in addition to Al accumulation, there was a proportional increase in the P concentration in the root tips of the UFVM-200 genotype. This is indicative of possible precipitation and/or immobilization of Al in the root tip apoplast of this genotype, which contributes to symplastic detoxification. |
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Effects of aluminum on the elongation and external morphology of root tips in two maize genotypesaluminum toleranceroot growthroot morphologyZea maysABSTRACT The objective of this study was to evaluate the effects of toxic levels of aluminum (Al) on the growth and external morphology of root tips in two maize genotypes with differential Al tolerance. The maize genotypes UFVM-100 (Al-sensitive) and UFVM-200 (Al-tolerant) were treated with 0 and 50 mM Al in a 0.5 mM CaCl2 solution at pH 4.5; root elongation, Al content and the external morphology of the root tips were evaluated. Chemical analysis, hematoxylin staining and energy dispersive X-ray spectrometry (EDS) showed greater Al accumulation in the root tips of the UFVM-200 genotype. The inhibition of root elongation in the UFVM-100 genotype, however, was much stronger than in the UFVM-200 genotype. Both maize genotypes exhibited visible and intense alterations in external micromorphology of the root tip, especially the Al-sensitive UFVM-100 genotype. Scanning electron micrographs showed intense cell disorganization and transverse ruptures of the protodermic and outer cortex layers of the cells in both genotypes. The ruptures were deeper and wider and reached the inner cortex layers in the UFVM-100 genotype. The EDS analysis showed that, in addition to Al accumulation, there was a proportional increase in the P concentration in the root tips of the UFVM-200 genotype. This is indicative of possible precipitation and/or immobilization of Al in the root tip apoplast of this genotype, which contributes to symplastic detoxification.Instituto Agronômico de Campinas2016-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052016000100019Bragantia v.75 n.1 2016reponame:Bragantiainstname:Instituto Agronômico de Campinas (IAC)instacron:IAC10.1590/1678-4499.142info:eu-repo/semantics/openAccessSouza,Leandro Torres deCambraia,JoséRibeiro,ClebersonOliveira,Juraci Alves deSilva,Luzimar Campos daeng2016-03-04T00:00:00Zoai:scielo:S0006-87052016000100019Revistahttps://www.scielo.br/j/brag/https://old.scielo.br/oai/scielo-oai.phpbragantia@iac.sp.gov.br||bragantia@iac.sp.gov.br1678-44990006-8705opendoar:2016-03-04T00:00Bragantia - Instituto Agronômico de Campinas (IAC)false |
dc.title.none.fl_str_mv |
Effects of aluminum on the elongation and external morphology of root tips in two maize genotypes |
title |
Effects of aluminum on the elongation and external morphology of root tips in two maize genotypes |
spellingShingle |
Effects of aluminum on the elongation and external morphology of root tips in two maize genotypes Souza,Leandro Torres de aluminum tolerance root growth root morphology Zea mays |
title_short |
Effects of aluminum on the elongation and external morphology of root tips in two maize genotypes |
title_full |
Effects of aluminum on the elongation and external morphology of root tips in two maize genotypes |
title_fullStr |
Effects of aluminum on the elongation and external morphology of root tips in two maize genotypes |
title_full_unstemmed |
Effects of aluminum on the elongation and external morphology of root tips in two maize genotypes |
title_sort |
Effects of aluminum on the elongation and external morphology of root tips in two maize genotypes |
author |
Souza,Leandro Torres de |
author_facet |
Souza,Leandro Torres de Cambraia,José Ribeiro,Cleberson Oliveira,Juraci Alves de Silva,Luzimar Campos da |
author_role |
author |
author2 |
Cambraia,José Ribeiro,Cleberson Oliveira,Juraci Alves de Silva,Luzimar Campos da |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Souza,Leandro Torres de Cambraia,José Ribeiro,Cleberson Oliveira,Juraci Alves de Silva,Luzimar Campos da |
dc.subject.por.fl_str_mv |
aluminum tolerance root growth root morphology Zea mays |
topic |
aluminum tolerance root growth root morphology Zea mays |
description |
ABSTRACT The objective of this study was to evaluate the effects of toxic levels of aluminum (Al) on the growth and external morphology of root tips in two maize genotypes with differential Al tolerance. The maize genotypes UFVM-100 (Al-sensitive) and UFVM-200 (Al-tolerant) were treated with 0 and 50 mM Al in a 0.5 mM CaCl2 solution at pH 4.5; root elongation, Al content and the external morphology of the root tips were evaluated. Chemical analysis, hematoxylin staining and energy dispersive X-ray spectrometry (EDS) showed greater Al accumulation in the root tips of the UFVM-200 genotype. The inhibition of root elongation in the UFVM-100 genotype, however, was much stronger than in the UFVM-200 genotype. Both maize genotypes exhibited visible and intense alterations in external micromorphology of the root tip, especially the Al-sensitive UFVM-100 genotype. Scanning electron micrographs showed intense cell disorganization and transverse ruptures of the protodermic and outer cortex layers of the cells in both genotypes. The ruptures were deeper and wider and reached the inner cortex layers in the UFVM-100 genotype. The EDS analysis showed that, in addition to Al accumulation, there was a proportional increase in the P concentration in the root tips of the UFVM-200 genotype. This is indicative of possible precipitation and/or immobilization of Al in the root tip apoplast of this genotype, which contributes to symplastic detoxification. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-03-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052016000100019 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052016000100019 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1678-4499.142 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto Agronômico de Campinas |
publisher.none.fl_str_mv |
Instituto Agronômico de Campinas |
dc.source.none.fl_str_mv |
Bragantia v.75 n.1 2016 reponame:Bragantia instname:Instituto Agronômico de Campinas (IAC) instacron:IAC |
instname_str |
Instituto Agronômico de Campinas (IAC) |
instacron_str |
IAC |
institution |
IAC |
reponame_str |
Bragantia |
collection |
Bragantia |
repository.name.fl_str_mv |
Bragantia - Instituto Agronômico de Campinas (IAC) |
repository.mail.fl_str_mv |
bragantia@iac.sp.gov.br||bragantia@iac.sp.gov.br |
_version_ |
1754193304572919808 |