Genetic diversity, population structure and AFLP markers associated with maize reaction to southern rust

Detalhes bibliográficos
Autor(a) principal: Giordani,Willian
Data de Publicação: 2019
Outros Autores: Scapim,Carlos Alberto, Ruas,Paulo Maurício, Ruas,Claudete de Fátima, Contreras-Soto,Rodrigo, Coan,Marlon, Fonseca,Inês Cristina de Batista, Gonçalves,Leandro Simões Azeredo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Bragantia
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052019000200183
Resumo: ABSTRACT Maize is one of the species with greater genetic diversity among cereals and possibly the most diverse crop species known. Accessing this variability is essential for maize breeding, allowing breeders to achieve progress of yield increasing, overcome environmental challenges or deal with pests and diseases. Among the maize diseases, southern rust is one of the most important, causing significant losses in yield and presenting severe epidemics worldwide. In the present study, the AFLP technique was applied to analyze population structure and genetic diversity among 145 tropical maize inbred lines, and to test for preliminary evidence of association between AFLP markers and the reaction to southern rust. Disease severity was evaluated in two crop seasons and the accessions were genotyped through AFLP using four primer combinations. The clusters obtained based on the Jaccard genetic distance and Ward’s hierarchical clustering and those achieved by structure simulations had high concordance and were capable of establish two big clusters, one predominantly of common maize and another of popcorn. The association analysis was performed using four different statistical models. The more complete model containing both population structure and genetic relatedness narrowed the number of significant associations, demonstrating its importance to control false associations. A total of 19 significant marker associations were identified from which three (EactMctg18, EactMctg205, EactMctg169) are interesting candidates for further investigations.
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spelling Genetic diversity, population structure and AFLP markers associated with maize reaction to southern rustZea maysgwasassociation studiescluster analysisgenetic distancePuccinia polysoraABSTRACT Maize is one of the species with greater genetic diversity among cereals and possibly the most diverse crop species known. Accessing this variability is essential for maize breeding, allowing breeders to achieve progress of yield increasing, overcome environmental challenges or deal with pests and diseases. Among the maize diseases, southern rust is one of the most important, causing significant losses in yield and presenting severe epidemics worldwide. In the present study, the AFLP technique was applied to analyze population structure and genetic diversity among 145 tropical maize inbred lines, and to test for preliminary evidence of association between AFLP markers and the reaction to southern rust. Disease severity was evaluated in two crop seasons and the accessions were genotyped through AFLP using four primer combinations. The clusters obtained based on the Jaccard genetic distance and Ward’s hierarchical clustering and those achieved by structure simulations had high concordance and were capable of establish two big clusters, one predominantly of common maize and another of popcorn. The association analysis was performed using four different statistical models. The more complete model containing both population structure and genetic relatedness narrowed the number of significant associations, demonstrating its importance to control false associations. A total of 19 significant marker associations were identified from which three (EactMctg18, EactMctg205, EactMctg169) are interesting candidates for further investigations.Instituto Agronômico de Campinas2019-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052019000200183Bragantia v.78 n.2 2019reponame:Bragantiainstname:Instituto Agronômico de Campinas (IAC)instacron:IAC10.1590/1678-4499.20180180info:eu-repo/semantics/openAccessGiordani,WillianScapim,Carlos AlbertoRuas,Paulo MaurícioRuas,Claudete de FátimaContreras-Soto,RodrigoCoan,MarlonFonseca,Inês Cristina de BatistaGonçalves,Leandro Simões Azeredoeng2019-06-27T00:00:00Zoai:scielo:S0006-87052019000200183Revistahttps://www.scielo.br/j/brag/https://old.scielo.br/oai/scielo-oai.phpbragantia@iac.sp.gov.br||bragantia@iac.sp.gov.br1678-44990006-8705opendoar:2019-06-27T00:00Bragantia - Instituto Agronômico de Campinas (IAC)false
dc.title.none.fl_str_mv Genetic diversity, population structure and AFLP markers associated with maize reaction to southern rust
title Genetic diversity, population structure and AFLP markers associated with maize reaction to southern rust
spellingShingle Genetic diversity, population structure and AFLP markers associated with maize reaction to southern rust
Giordani,Willian
Zea mays
gwas
association studies
cluster analysis
genetic distance
Puccinia polysora
title_short Genetic diversity, population structure and AFLP markers associated with maize reaction to southern rust
title_full Genetic diversity, population structure and AFLP markers associated with maize reaction to southern rust
title_fullStr Genetic diversity, population structure and AFLP markers associated with maize reaction to southern rust
title_full_unstemmed Genetic diversity, population structure and AFLP markers associated with maize reaction to southern rust
title_sort Genetic diversity, population structure and AFLP markers associated with maize reaction to southern rust
author Giordani,Willian
author_facet Giordani,Willian
Scapim,Carlos Alberto
Ruas,Paulo Maurício
Ruas,Claudete de Fátima
Contreras-Soto,Rodrigo
Coan,Marlon
Fonseca,Inês Cristina de Batista
Gonçalves,Leandro Simões Azeredo
author_role author
author2 Scapim,Carlos Alberto
Ruas,Paulo Maurício
Ruas,Claudete de Fátima
Contreras-Soto,Rodrigo
Coan,Marlon
Fonseca,Inês Cristina de Batista
Gonçalves,Leandro Simões Azeredo
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Giordani,Willian
Scapim,Carlos Alberto
Ruas,Paulo Maurício
Ruas,Claudete de Fátima
Contreras-Soto,Rodrigo
Coan,Marlon
Fonseca,Inês Cristina de Batista
Gonçalves,Leandro Simões Azeredo
dc.subject.por.fl_str_mv Zea mays
gwas
association studies
cluster analysis
genetic distance
Puccinia polysora
topic Zea mays
gwas
association studies
cluster analysis
genetic distance
Puccinia polysora
description ABSTRACT Maize is one of the species with greater genetic diversity among cereals and possibly the most diverse crop species known. Accessing this variability is essential for maize breeding, allowing breeders to achieve progress of yield increasing, overcome environmental challenges or deal with pests and diseases. Among the maize diseases, southern rust is one of the most important, causing significant losses in yield and presenting severe epidemics worldwide. In the present study, the AFLP technique was applied to analyze population structure and genetic diversity among 145 tropical maize inbred lines, and to test for preliminary evidence of association between AFLP markers and the reaction to southern rust. Disease severity was evaluated in two crop seasons and the accessions were genotyped through AFLP using four primer combinations. The clusters obtained based on the Jaccard genetic distance and Ward’s hierarchical clustering and those achieved by structure simulations had high concordance and were capable of establish two big clusters, one predominantly of common maize and another of popcorn. The association analysis was performed using four different statistical models. The more complete model containing both population structure and genetic relatedness narrowed the number of significant associations, demonstrating its importance to control false associations. A total of 19 significant marker associations were identified from which three (EactMctg18, EactMctg205, EactMctg169) are interesting candidates for further investigations.
publishDate 2019
dc.date.none.fl_str_mv 2019-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052019000200183
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052019000200183
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1678-4499.20180180
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Agronômico de Campinas
publisher.none.fl_str_mv Instituto Agronômico de Campinas
dc.source.none.fl_str_mv Bragantia v.78 n.2 2019
reponame:Bragantia
instname:Instituto Agronômico de Campinas (IAC)
instacron:IAC
instname_str Instituto Agronômico de Campinas (IAC)
instacron_str IAC
institution IAC
reponame_str Bragantia
collection Bragantia
repository.name.fl_str_mv Bragantia - Instituto Agronômico de Campinas (IAC)
repository.mail.fl_str_mv bragantia@iac.sp.gov.br||bragantia@iac.sp.gov.br
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